• Clinical and Experimental Reproductive Medicine
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• 제26권2호
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• pp.257-263
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• 1999

It is well known that discard of seminal plasma from the semen and separation of motile sperm should be preceded before insemination for IUI or IVF. Till now, more than ten kinds of semen treatment methods have been developed. Of those, swim-up and Percoll methods have been used widely in ART laboratories as a routine semen treatment methods because of its advantages. However, there are reports that Percoll can make a genetic trouble because of its chemical structure and therefore the necessity has been arisen to substitute Percoll for other equivalent materials. This study was performed to evaluate the effects of three different sperm preparation methods (swim-up, Percoll and Sil-Select) on sperm motility, sperm recovery rate and fertilization rate. Also, the feasibility of using Sil-Select instead of Percoll in ART was evaluated. Each semen samples were divided into three fractions and motile sperm were recovered by swim-up, Percoll and Sil-Select gradient centrifugation methods. Normal and sub-normal criteria of fifteen semen samples and seventeen IVF cycles were included in these study. As results, no significant difference was found in sperm recovery rate in normal semen treated by a Swim-up, Percoll and Sil-Select method ($13.2{\times}10^6,\;17.5{\times}10^6\;and\;17.7{\times}10^6$ respectively). The initial sperm motility was 61.9% and this increased to 87.1%, 92.6% and 89.5% through Swim-up, Percoll and Sil-Select treatment, respectively. Higher motility was observed in Percoll and Sil-Select treated groups (81.5%, 79.2%, respectively) than swim-up group (66.8%) after incubation for 24hrs. In sub-normal group, sperm recovery rates were higher in Sil-Select group $(2.9{\times}10^6)$ than Percoll gradients group $(1.8{\times}10^6)$. In IVF cycles, the outcomes of fertilization using sperm treated by swim-up and Sil-Select group were similar (82.2%, 79.7% respectively). In conclusion, our results indicate that Sil-Select can be used as a substitute material for sperm preparation instead of Percoll.

• Clinical and Experimental Reproductive Medicine
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• 제27권4호
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• pp.339-344
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• 2000

Objective: To evaluate silane-coated silica particles (Sil-select) as an alternative to polyvinylpyrrolidone-coated particles (Percoll) for gradient separation of spermatozoa, for use in assisted reproduction. Methods: 20 normal semen based on WHO criteria were included in this study. Recovery of motile and morphologically normal spermatozoa after using two-layer Percoll and Sil-select gradient respectively was recorded. Motility, HOST (hypoosmotic swelling test) and the detection of malondialdehyde for LPO (lipid peroxidation) after 24 h of incubation at $37^{\circ}C$ in a 5% $CO_2$ incubator were compared. Results: Percoll (78.5%) and Sil-select (79.1%) showed a significant increase in the motility compared to ejaculate (60.9%) but no difference between Percoll and Sil-select. Normal sperm morphology significantly increased after Percoll (57.6%) and Sil-select (53.7%) compared to ejaculate (35.8%) but no difference between Percoll and Sil-select. No differences in the recovery of motile spermatozoa and motility, HOST and the production of malondialdehyde after 24 h incubation were found when comparing the use of Percoll and Sil-select. Conclusion: Sil-select seems to be an attractive alternative to Percoll for sperm separation in assisted reproduction.

• 대한전기학회:학술대회논문집
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• 대한전기학회 1997년도 하계학술대회 논문집 D
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• pp.948-950
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• 1997

This paper presents a basic interposing logic program to control nuclear power plant. In this paper we select a target control loop among the whole interposing modules, develop logic algorithm and functional software to compose target control loop. After that we carry out V&V(Verification and Validation) into the developed logic program to improve quality and reliability.

• 조명전기설비학회논문지
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• 제29권7호
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• pp.47-53
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• 2015

As the industrial development in Korea, the power demand is rapidly increased. The power system has been extended and complicated to meet the power demand. However, the fault current increases because the structure of power system reduces the equivalent impedance. One of the solutions to reduce the fault current is Superconducting Fault Current Limiter(SFCL) and the researches of SFCL application have much conducted. In this paper, we used the Circuit Breker Burden Index(CBBI) to select application location of SFCL because the CBBI may consider a fault current of each line. In addition, this paper divided the weight factor used to calculate the CBBI on a case by case basis and analyzed this characteristics through experiment.

• 한국컴퓨터정보학회논문지
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• 제17권11호
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• pp.149-161
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• 2012

인터넷 및 소셜 네트워크 등이 적극적으로 활용되는 사용자 참여 사회에서 사용자의 요구사항을 올바르게 시스템에 반영하는 것은 매우 중요하다. 본 연구에서는 C 사의1,800 여건의사용자 요구사항을 정형화하기 위한 절차와 기법을 제안하고, 정형화된 요구사항이 시스템 설계와 구현에 반영될 수 있도록 상대적 기여도를 측정하여 선정하는 기법을 제시한다. 다양한 사용자 요구사항을 요구사항 수준에 적합하도록 정형화하기 위하여, 요구사항 명세 표준에 따라 작성하고 키워드를 추출하고 상호 관계를 분석하여, 요구사항 계층 그래프를 작성하였다. 요구사항 계층 그래프는 시스템 설계와 구현을 위한 세부 목표를 정의하며, 계층별 요구사항의 상대적인 중요도를 표현한다. 요구사항의 상대적 중요도를 이용하여 요구사항별 상대적 기여도를 계산하고, 이를 이용한 선정 평가 기법을 제안한다. 제안한 기법의 타당성은 C 사의 사례 연구를 통하여 그 가능성을 입증하였다.

• 한국산학기술학회논문지
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• 제20권6호
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• pp.541-547
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• 2019

최근 자동화 되는 해킹 및 분석 기술의 발전으로 인하여 수많은 소프트웨어 보안 취약점이 빠르게 발표되고 있다. 대표적인 취약점 데이터베이스인 NVD(National Vulnerability Database)에는 2010년부터 2015년까지 보안 취약점(CVE: Common Vulnerability Enumeration) 약 8만 건이 등록되었으며, 최근에도 점차 증가하고 있는 추세이다. 보안 취약점은 빠른 속도로 증가하고 있는 반면, 보안 취약점을 분석하고 대응하는 방법은 전문가의 수동 분석에 의존하고 있어 대응 속도가 느리다. 이런 문제점을 해결하기 위해 자동화된 방법으로 보안 취약점을 탐색하고, 패치하여 악의적인 공격자에게 공격 기회를 줄 수 있는 보안 취약점을 사전에 대응 할 수 있는 기술이 필요하다. 본 논문에서는 복잡도 분석을 통해 취약점 탐색 대상 바이너리의 특징을 추출하고, 특징에 적합한 취약점 탐색 전략을 선정하여 취약점을 자동으로 탐색하는 기술을 제안한다. 제안 기술은 AFL, ANGR, Driller 도구와 비교 검증 하였으며 코드 커버리지는 약 6% 향상, 크래시 개수는 약 2.4배 증가, 크래시 발생율 약 11% 향상 효과를 볼 수 있었다.

• Journal of Plant Biotechnology
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• 제36권3호
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• pp.219-223
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• 2009

Oxidative stress derived from excess reactive oxygen species (ROS) is a major damaging factor for plants exposed to environmental stresses. Sweetpotato [Ipomoea batatas (L.) Lam] has a relatively broad adaptability to harsh environmental conditions compared to other staple crops. In this study, to select stress-tolerant sweetpotato cultivars for sources of molecular breeding on marginal lands, we evaluated the ion leakage values in 10 different cultivars after treatment of methyl viologen (MV), an ROS-generating nonselective herbicide, to leaf discs. DPPH radical scavenging activity and the contents of total phenolics were also investigated. The ion leakage of each cultivar showed a diverse value, which is well correlated with DPPH radical scavenging activity of each cultivar. DPPH radical scavenging activity also showed a high corelation with the contents of total phenolic contents. Three cultivars of Yanshu 8, Shinhwangmi and Shinzami showed high antioxidant activity. Our results suggest that a simple and efficient DPPH radical scavenging activity would be a suitable method to select potential cultivars with enhanced tolerance to multiple environmental stress.

• 대한전자공학회:학술대회논문집
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• 대한전자공학회 2004년도 학술대회지
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• pp.743-746
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• 2004

This study is generally purposed to develop a mobile augmentative and alternative communication system (hereinafter referred to as 'a mobile AAC system'). Also the device is aimed as a mobile AAC system for helping communication of persons who suffer from speech disorders in a free and convenient manner. One thing firstly considered for a successful AAC system is selection of vocabularies. For this purpose, the above researchers collected utterances in actual situations to select vocabularies of higher frequencies. For generation of sentences by maximizing the use of limited space in the AAC system, this study specifically presents a method of predicting predictions. This method includes selecting vocabulary and classifying it by domains so as to meet the characteristics of the mobile AAC communication, using a noun thesaurus for semantic analysis, and building a sub-category dictionary. Predicting predicates by selecting symbols in accordance with this method is tested and the utility thereof is confirmed.

• 사상체질의학회지
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• 제13권3호
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• pp.8-14
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• 2001

1. Purpose A branch of acupuncture is to be tender at the sasang contitutional medicine. So studying 『HwangJeNaiKyung』, we find the commencement of sasangin-acupuncture that Dong-Mu was not discovered. 2. Method We select the constitutional acupuncture of 『HwangJeNaiKyung』, and analysis that. 3. Result We can find the constitutional acupuncture of 『HwangJeNaiKyung』 as follows. The first, Acu-point of each constitution is not mentioned. The second, Acupuncture comparing with HyungJiGoRak(形志苦樂) of 『HwangJeNaiKyung』 is effective at mind-treatment. The third, the response of acupuncture supply the commencement that can devide YangIn(陽人) and EumIn(陰人). The forth, according to the fatness and the thinness it must be different with the depth of acupuncture and the time of acupuncture. The fifth, according to the HanYeulHeuSil(寒熱虛實) of constitution, there can be different the depth of acupuncture, the time of acupuncture and the count of acupuncture. But throuth 『HwangJeNaiKyung』 we can not find the commencement of the theory of Dong-Mu's SeungGangWanSok(升降緩束).

• Toxicological Research
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• 제32권3호
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• pp.195-205
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• 2016

This study was performed to select single nucleotide polymorphisms (SNPs) related to the body burden of heavy metals in Koreans, to provide Korean allele frequencies of selected SNPs, and to assess the difference in allele frequencies with other ethnicities. The candidate-gene approach method and genome-wide association screening were used to select SNPs related to the body burden of heavy metals. Genotyping analysis of the final 192 SNPs selected was performed on 1,483 subjects using the VeraCode Goldengate assay. Allele frequencies differences and genetic differentiations between the Korean population and Chinese (CHB), Japanese (JPT), Caucasian (CEU), and African (YIR) populations were tested by Fisher's exact test and fixation index ($F_{ST}$), respectively. The Korean population was genetically similar to the CHB and JPT populations ($F_{ST}$ < 0.05, for all SNPs in both populations). However, a significant difference in the allele frequencies between the Korean and CEU and YIR populations were observed in 99 SNPs (60.7%) and 120 SNPs (73.6%), respectively. Ten (6.1%) and 26 (16.0%) SNPs had genetic differentiation ($F_{ST}$ > 0.05) among the Korean-CEU and Korean-YIR comparisons, respectively. The SNP with the largest $F_{ST}$ value between the Korean and African populations was cystathionine-${\beta}$-synthase rs234709 ($F_{ST}$: KOR-YIR, 0.309; KOR-CEU, 0.064). Our study suggests that interethnic differences exist in SNPs associated with heavy metals of Koreans, and it should be considered in future studies that address ethnic differences in heavy-metal concentrations in the body and genetic susceptibility to the body burden of heavy metals.