• Title/Summary/Keyword: Sil-Select

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Comparison of Sperm Motility, Recovery Rate, and Fertilization Rate using Three Different Sperm Preparation Methods: Swim-up, Percoll, Sit-Select (Swim-up, Percoll, Sil-Select를 이용한 정자처리법에 의한 정자회수율, 운동성 및 체외수정율의 비교분석)

  • Ha, J.H.;Oum, K.B.;Chung, H.M.;Chung, M.K.;Kim, H.K.;Ko, J.J.;Yoon, T.K.;Cha, K.Y.
    • Clinical and Experimental Reproductive Medicine
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    • v.26 no.2
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    • pp.257-263
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    • 1999
  • It is well known that discard of seminal plasma from the semen and separation of motile sperm should be preceded before insemination for IUI or IVF. Till now, more than ten kinds of semen treatment methods have been developed. Of those, swim-up and Percoll methods have been used widely in ART laboratories as a routine semen treatment methods because of its advantages. However, there are reports that Percoll can make a genetic trouble because of its chemical structure and therefore the necessity has been arisen to substitute Percoll for other equivalent materials. This study was performed to evaluate the effects of three different sperm preparation methods (swim-up, Percoll and Sil-Select) on sperm motility, sperm recovery rate and fertilization rate. Also, the feasibility of using Sil-Select instead of Percoll in ART was evaluated. Each semen samples were divided into three fractions and motile sperm were recovered by swim-up, Percoll and Sil-Select gradient centrifugation methods. Normal and sub-normal criteria of fifteen semen samples and seventeen IVF cycles were included in these study. As results, no significant difference was found in sperm recovery rate in normal semen treated by a Swim-up, Percoll and Sil-Select method ($13.2{\times}10^6,\;17.5{\times}10^6\;and\;17.7{\times}10^6$ respectively). The initial sperm motility was 61.9% and this increased to 87.1%, 92.6% and 89.5% through Swim-up, Percoll and Sil-Select treatment, respectively. Higher motility was observed in Percoll and Sil-Select treated groups (81.5%, 79.2%, respectively) than swim-up group (66.8%) after incubation for 24hrs. In sub-normal group, sperm recovery rates were higher in Sil-Select group $(2.9{\times}10^6)$ than Percoll gradients group $(1.8{\times}10^6)$. In IVF cycles, the outcomes of fertilization using sperm treated by swim-up and Sil-Select group were similar (82.2%, 79.7% respectively). In conclusion, our results indicate that Sil-Select can be used as a substitute material for sperm preparation instead of Percoll.

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Comparison between Percoll and Sil-Select Methods on the Human Spermatozoa Treatment (인간정자의 처리에 있어서 Percoll과 Sil-Select 방법의 비교)

  • Moon, Shin-Yong;Ryu, Buom-Yong;Shin, Hyun-Ah;Oh, Sun-Kyung;Suh, Chang-Suk;Kim, Seok-Hyun;Choi, Young-Min;Kim, Jung-Gu;Choi, Kyu-Hong;Lee, Jin-Yong
    • Clinical and Experimental Reproductive Medicine
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    • v.27 no.4
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    • pp.339-344
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    • 2000
  • Objective: To evaluate silane-coated silica particles (Sil-select) as an alternative to polyvinylpyrrolidone-coated particles (Percoll) for gradient separation of spermatozoa, for use in assisted reproduction. Methods: 20 normal semen based on WHO criteria were included in this study. Recovery of motile and morphologically normal spermatozoa after using two-layer Percoll and Sil-select gradient respectively was recorded. Motility, HOST (hypoosmotic swelling test) and the detection of malondialdehyde for LPO (lipid peroxidation) after 24 h of incubation at $37^{\circ}C$ in a 5% $CO_2$ incubator were compared. Results: Percoll (78.5%) and Sil-select (79.1%) showed a significant increase in the motility compared to ejaculate (60.9%) but no difference between Percoll and Sil-select. Normal sperm morphology significantly increased after Percoll (57.6%) and Sil-select (53.7%) compared to ejaculate (35.8%) but no difference between Percoll and Sil-select. No differences in the recovery of motile spermatozoa and motility, HOST and the production of malondialdehyde after 24 h incubation were found when comparing the use of Percoll and Sil-select. Conclusion: Sil-select seems to be an attractive alternative to Percoll for sperm separation in assisted reproduction.

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Development of Logic Program for Nuclear Power Plants Control (원자력 발전용 플랜트 제어를 위한 로직 프로그램의 개발)

  • Kim, Young-Chun;Yoon, Yong-Han;Kim, Jae-Chul;Hwang, Sun-Ju;Lee, Yong-Gil;Park, Chang-Du
    • Proceedings of the KIEE Conference
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    • 1997.07c
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    • pp.948-950
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    • 1997
  • This paper presents a basic interposing logic program to control nuclear power plant. In this paper we select a target control loop among the whole interposing modules, develop logic algorithm and functional software to compose target control loop. After that we carry out V&V(Verification and Validation) into the developed logic program to improve quality and reliability.

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Analysis on Application Location of SFCL according to Circuit Breaker Burden Index with Weight Factor Estimation (차단기 부담 평가지표의 가중치 산정에 따른 초전도 한류기 적용위치 분석)

  • Park, Han-Min;Lee, Hyeong-Jin;Kim, Jin-Seok;Lim, Sung-Hun;Kim, Jae-Chul
    • Journal of the Korean Institute of Illuminating and Electrical Installation Engineers
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    • v.29 no.7
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    • pp.47-53
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    • 2015
  • As the industrial development in Korea, the power demand is rapidly increased. The power system has been extended and complicated to meet the power demand. However, the fault current increases because the structure of power system reduces the equivalent impedance. One of the solutions to reduce the fault current is Superconducting Fault Current Limiter(SFCL) and the researches of SFCL application have much conducted. In this paper, we used the Circuit Breker Burden Index(CBBI) to select application location of SFCL because the CBBI may consider a fault current of each line. In addition, this paper divided the weight factor used to calculate the CBBI on a case by case basis and analyzed this characteristics through experiment.

A Technique for Requirements Normalization and Selection based on Practical Approach (사례 기반의 요구사항 정형화 및 선정 평가 기법)

  • Byun, Jung-Won;Rhew, Sung-Yul;Kim, Jin-Su
    • Journal of the Korea Society of Computer and Information
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    • v.17 no.11
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    • pp.149-161
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    • 2012
  • In the customer-centered world, which is used internet and social network services actively, it is important that their needs should be embraced into systems. Our study proposes a technique to normalize and select 1,800 customer's needs at C company. In order to normalize the requirements, we have specified requirements by considering a standard for requirements specification, have identified a set of keywords of requirements and have constructed a relation-graph of requirements. The graph presents objectives to design and build a system, and shows a degree of relative importance for each requirement. And then, we propose a techniques for requirements selection according to their contribution points, which are calculated using relative degree. We demonstrates our techniques by a case study of C company.

A Study on Hybrid Fuzzing using Dynamic Analysis for Automatic Binary Vulnerability Detection (바이너리 취약점의 자동 탐색을 위한 동적분석 정보 기반 하이브리드 퍼징 연구)

  • Kim, Taeeun;Jurn, Jeesoo;Jung, Yong Hoon;Jun, Moon-Seog
    • Journal of the Korea Academia-Industrial cooperation Society
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    • v.20 no.6
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    • pp.541-547
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    • 2019
  • Recent developments in hacking technology are continuing to increase the number of new security vulnerabilities. Approximately 80,000 new vulnerabilities have been registered in the Common Vulnerability Enumeration (CVE) database, which is a representative vulnerability database, from 2010 to 2015, and the trend is gradually increasing in recent years. While security vulnerabilities are growing at a rapid pace, responses to security vulnerabilities are slow to respond because they rely on manual analysis. To solve this problem, there is a need for a technology that can automatically detect and patch security vulnerabilities and respond to security vulnerabilities in advance. In this paper, we propose the technology to extract the features of the vulnerability-discovery target binary through complexity analysis, and select a vulnerability-discovery strategy suitable for the feature and automatically explore the vulnerability. The proposed technology was compared to the AFL, ANGR, and Driller tools, with about 6% improvement in code coverage, about 2.4 times increase in crash count, and about 11% improvement in crash incidence.

Selection of oxidative stress-tolerant sweetpotato cultivars for cultivation on marginal lands (조건불리지역 재배를 위한 산화스트레스 내성 고구마 품종의 선발)

  • Kim, Yun-Hee;Park, Sung-Chul;Yang, Kyoung-Sil;Zhou, Zhilin;Zhao, Donglan;Ma, Daifu;Jeong, Jae-Cheol;Lee, Haeng-Soon;Kwak, Sang-Soo
    • Journal of Plant Biotechnology
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    • v.36 no.3
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    • pp.219-223
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    • 2009
  • Oxidative stress derived from excess reactive oxygen species (ROS) is a major damaging factor for plants exposed to environmental stresses. Sweetpotato [Ipomoea batatas (L.) Lam] has a relatively broad adaptability to harsh environmental conditions compared to other staple crops. In this study, to select stress-tolerant sweetpotato cultivars for sources of molecular breeding on marginal lands, we evaluated the ion leakage values in 10 different cultivars after treatment of methyl viologen (MV), an ROS-generating nonselective herbicide, to leaf discs. DPPH radical scavenging activity and the contents of total phenolics were also investigated. The ion leakage of each cultivar showed a diverse value, which is well correlated with DPPH radical scavenging activity of each cultivar. DPPH radical scavenging activity also showed a high corelation with the contents of total phenolic contents. Three cultivars of Yanshu 8, Shinhwangmi and Shinzami showed high antioxidant activity. Our results suggest that a simple and efficient DPPH radical scavenging activity would be a suitable method to select potential cultivars with enhanced tolerance to multiple environmental stress.

Implementation of AAC System for Persons Suffering from Speech Disorders

  • Lee Eun Sil;Hur Tai Sung;Park Young Bae;Woo Yo Seop;Min Hong Ki
    • Proceedings of the IEEK Conference
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    • 2004.08c
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    • pp.743-746
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    • 2004
  • This study is generally purposed to develop a mobile augmentative and alternative communication system (hereinafter referred to as 'a mobile AAC system'). Also the device is aimed as a mobile AAC system for helping communication of persons who suffer from speech disorders in a free and convenient manner. One thing firstly considered for a successful AAC system is selection of vocabularies. For this purpose, the above researchers collected utterances in actual situations to select vocabularies of higher frequencies. For generation of sentences by maximizing the use of limited space in the AAC system, this study specifically presents a method of predicting predictions. This method includes selecting vocabulary and classifying it by domains so as to meet the characteristics of the mobile AAC communication, using a noun thesaurus for semantic analysis, and building a sub-category dictionary. Predicting predicates by selecting symbols in accordance with this method is tested and the utility thereof is confirmed.

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A Literature Study on the Treatment of Acupuncture for the Constitution - Through the Study for the Detail of 『HwangJeNaiKyung』 (黃帝內經) - (체질(體質)에 따른 침치료(針治療)를 위한 문헌적(文獻的) 고찰(考察) - 『황제내경(黃帝內經)』을 중심으로 -)

  • Han, Kyung-suk;Park, Seong-sik
    • Journal of Sasang Constitutional Medicine
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    • v.13 no.3
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    • pp.8-14
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    • 2001
  • 1. Purpose A branch of acupuncture is to be tender at the sasang contitutional medicine. So studying 『HwangJeNaiKyung』, we find the commencement of sasangin-acupuncture that Dong-Mu was not discovered. 2. Method We select the constitutional acupuncture of 『HwangJeNaiKyung』, and analysis that. 3. Result We can find the constitutional acupuncture of 『HwangJeNaiKyung』 as follows. The first, Acu-point of each constitution is not mentioned. The second, Acupuncture comparing with HyungJiGoRak(形志苦樂) of 『HwangJeNaiKyung』 is effective at mind-treatment. The third, the response of acupuncture supply the commencement that can devide YangIn(陽人) and EumIn(陰人). The forth, according to the fatness and the thinness it must be different with the depth of acupuncture and the time of acupuncture. The fifth, according to the HanYeulHeuSil(寒熱虛實) of constitution, there can be different the depth of acupuncture, the time of acupuncture and the count of acupuncture. But throuth 『HwangJeNaiKyung』 we can not find the commencement of the theory of Dong-Mu's SeungGangWanSok(升降緩束).

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Allele Frequencies of the Single Nucleotide Polymorphisms Related to the Body Burden of Heavy Metals in the Korean Population and Their Ethnic Differences

  • Eom, Sang-Yong;Lim, Ji-Ae;Kim, Yong-Dae;Choi, Byung-Sun;Hwang, Myung Sil;Park, Jung-Duck;Kim, Heon;Kwon, Ho-Jang
    • Toxicological Research
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    • v.32 no.3
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    • pp.195-205
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    • 2016
  • This study was performed to select single nucleotide polymorphisms (SNPs) related to the body burden of heavy metals in Koreans, to provide Korean allele frequencies of selected SNPs, and to assess the difference in allele frequencies with other ethnicities. The candidate-gene approach method and genome-wide association screening were used to select SNPs related to the body burden of heavy metals. Genotyping analysis of the final 192 SNPs selected was performed on 1,483 subjects using the VeraCode Goldengate assay. Allele frequencies differences and genetic differentiations between the Korean population and Chinese (CHB), Japanese (JPT), Caucasian (CEU), and African (YIR) populations were tested by Fisher's exact test and fixation index ($F_{ST}$), respectively. The Korean population was genetically similar to the CHB and JPT populations ($F_{ST}$ < 0.05, for all SNPs in both populations). However, a significant difference in the allele frequencies between the Korean and CEU and YIR populations were observed in 99 SNPs (60.7%) and 120 SNPs (73.6%), respectively. Ten (6.1%) and 26 (16.0%) SNPs had genetic differentiation ($F_{ST}$ > 0.05) among the Korean-CEU and Korean-YIR comparisons, respectively. The SNP with the largest $F_{ST}$ value between the Korean and African populations was cystathionine-${\beta}$-synthase rs234709 ($F_{ST}$: KOR-YIR, 0.309; KOR-CEU, 0.064). Our study suggests that interethnic differences exist in SNPs associated with heavy metals of Koreans, and it should be considered in future studies that address ethnic differences in heavy-metal concentrations in the body and genetic susceptibility to the body burden of heavy metals.