• Experimental and Molecular Medicine
• /
• 제50권12호
• /
• pp.3.1-3.14
• /
• 2018

Type 1 diabetes mellitus (T1DM) is a pathological condition associated with osteopenia. $WNT/{\beta}$-catenin signaling is implicated in this process. Trabecular and cortical bone respond differently to $WNT/{\beta}$-catenin signaling in healthy mice. We investigated whether this signaling has different effects on trabecular and cortical bone in T1DM. We first established a streptozotocin-induced T1DM mouse model and then constitutively activated ${\beta}$-catenin in osteoblasts in the setting of T1DM (T1-CA). The extent of bone loss was greater in trabecular bone than that in cortical bone in T1DM mice, and this difference was consistent with the reduction in the expression of ${\beta}$-catenin signaling in the two bone compartments. Further experiments demonstrated that in T1DM mice, trabecular bone showed lower levels of insulin-like growth factor-1 receptor (IGF-1R) than the levels in cortical bone, leading to lower $WNT/{\beta}$-catenin signaling activity through the inhibition of the IGF-1R/Akt/glycogen synthase kinase $3{\beta}$ ($GSK3{\beta}$) pathway. After ${\beta}$-catenin was activated in T1-CA mice, the bone mass and bone strength increased to substantially greater extents in trabecular bone than those in cortical bone. In addition, the cortical bone of the T1-CA mice displayed an unexpected increase in bone porosity, with increased bone resorption. The downregulated expression of WNT16 might be responsible for these cortical bone changes. In conclusion, we found that although the activation of $WNT/{\beta}$-catenin signaling increased the trabecular bone mass and bone strength in T1DM mice, it also increased the cortical bone porosity, impairing the bone strength. These findings should be considered in the future treatment of T1DM-related osteopenia.

• Journal of Ginseng Research
• /
• 제43권3호
• /
• pp.475-481
• /
• 2019

Background: The ginsenoside Rg1 has been shown to exert various pharmacological activities with health benefits. Previously, we have reported that Rg1 promoted myogenic differentiation and myotube growth in C2C12 myoblasts. In this study, the in vivo effect of Rg1 on fiber-type composition and oxidative metabolism in skeletal muscle was examined. Methods: To examine the effect of Rg1 on skeletal muscle, 3-month-old mice were treated with Rg1 for 5 weeks. To assess muscle strength, grip strength tests were performed, and the lower hind limb muscles were harvested, followed by various detailed analysis, such as histological staining, immunoblotting, immunostaining, and real-time quantitative reverse transcription polymerase chain reaction. In addition, to verify the in vivo data, primary myoblasts isolated from mice were treated with Rg1, and the Rg1 effect on myotube growth was examined by immunoblotting and immunostaining analysis. Results: Rg1 treatment increased the expression of myosin heavy chain isoforms characteristic for both oxidative and glycolytic muscle fibers; increased myofiber sizes were accompanied by enhanced muscle strength. Rg1 treatment also enhanced oxidative muscle metabolism with elevated oxidative phosphorylation proteins. Furthermore, Rg1-treated muscles exhibited increased levels of anabolic S6 kinase signaling. Conclusion: Rg1 improves muscle functionality via enhancing muscle gene expression and oxidative muscle metabolism in mice.

• Asian-Australasian Journal of Animal Sciences
• /
• 제26권5호
• /
• pp.609-624
• /
• 2013

The objective of this study was to get a comprehensive understanding of how genes in chicken shell gland modulate eggshell strength at the early stage of active calcification. Four 32-week old of purebred Xianju hens with consistent high or low shell breakage strength were grouped into two pairs. Using Affymetrix Chicken Array, a whole-transcriptome analysis was performed on hen's shell gland at 9 h post oviposition. Gene ontology enrichment analysis for differentially expressed (DE) transcripts was performed using the web-based GOEAST, and the validation of DE-transcripts was tested by qRT-PCR. 1,195 DE-transcripts, corresponding to 941 unique genes were identified in hens with strong eggshell compared to weak shell hens. According to gene ontology annotations, there are 77 DE-transcripts encoding ion transporters and secreted extracellular matrix proteins, and at least 26 DE-transcripts related to carbohydrate metabolism or post-translation glycosylation modification; furthermore, there are 88 signaling DE-transcripts. GO term enrichment analysis suggests that some DE-transcripts mediate reproductive hormones or neurotransmitters to affect eggshell quality through a complex suite of biophysical processes. These results reveal some candidate genes involved with eggshell strength at the early stage of active calcification which may facilitate our understanding of regulating mechanisms of eggshell quality.

• The Korean Journal of Physiology and Pharmacology
• /
• 제20권3호
• /
• pp.287-295
• /
• 2016

Involuntary physical activity induced by the avoidance of electrical shock leads to improved endurance exercise capacity in animals. However, it remains unknown whether voluntary stand-up physical activity (SPA) without forced simulating factors improves endurance exercise capacity in animals. We examined the effects of SPA on body weight, cardiac function, and endurance exercise capacity for 12 weeks. Twelve male Sprague-Dawley rats (aged 8 weeks, n=6 per group) were randomly assigned to a control group (CON) or a voluntary SPA group. The rats were induced to perform voluntary SPA (lifting a load equal to their body weight), while the food height (18.0 cm) in cages was increased progressively by 3.5 every 4 weeks until it reached 28.5 cm for 12 weeks. The SPA group showed a lower body weight compared to the CON group, but voluntary SPA did not affect the skeletal muscle and heart weights, food intake, and echocardiography results. Although the SPA group showed higher grip strength, running time, and distance compared to the CON group, the level of irisin, corticosterone, genetic expression of mitochondrial biogenesis, and nuclei numbers were not affected. These findings show that voluntary SPA without any forced stimuli in rats can effectively reduce body weight and enhance endurance exercise capacity, suggesting that it may be an important alternative strategy to enhance endurance exercise capacity.

• 정보처리학회논문지C
• /
• 제14C권7호
• /
• pp.603-610
• /
• 2007

이동 중인 사용자에게 연속성 있는 서비스 제공을 위해서는 효과적인 핸드오버 결정 기법이 필요하다. 본 논문에서는 측정된 수신 신호 강도를 이용한 핸드오버 결정 방법의 성능을 늦은 통보와 오보율 측면에서 평가하기 위한 분석 모델을 제안한다. 제안 모델은 핸드오버 결정 기법의 성능에 영향을 미치는 사용자의 이동성, 핸드오버 프로토콜 메시지 교환 지연, 셀 크기 및 안정적인 수신 신호 강도 측정을 위한 수신 신호 강도 측정 주기를 모두 고려한다. 또한 본 논문에서는 제안한 분석 모델을 통해 각 인자들의 영향을 정량적으로 고찰하여 효과적인 고속 핸드오버 시작 시점 결정을 위한 연구 방향을 제시한다.

• Computers and Concrete
• /
• 제21권3호
• /
• pp.239-248
• /
• 2018

This study developed and investigated a precast slab track system partially reinforced with glass fiber reinforced polymer (GFRP) rebars in the transverse direction, which mitigated the loss of track circuit current by reducing magnetic coupling between the rails and steel reinforcements. An electric analysis was conducted and the results of the analysis verified that the GFRP rebars mitigate the reduced current strength produced by electro-magnetic induction. In the study, a three-dimensional finite element method and flexural experiments were used to study the mechanical behavior of the proposed slab track.

• Toxicological Research
• /
• 제16권1호
• /
• pp.63-66
• /
• 2000

The effects of acute ethanol on the high K+ induced $Ca^{2+}}$ signals were examined from primary cultures of cerebellar granule neurons. $Ca^{2+}}$ signals were measured with Calcium Green-1 based microscopic video imaging. Because $Ca^{2+}}$ signal was low in most of granule neurons without stimuli, high KCI was used for depolarization. In most case, acute exposure to ethanol reduced the peak amplitude of the $Ca^{2+}}$ signals, induced by high K+, even though low concentration of ethanol(2~10mM) was used and the effects lasted more than 30min. In was also possible to see differences of ethanol inhibition, i.e. the temporal pattern of $Ca^{2+}}$ signal reductions and the strength of inhibition of $Ca^{2+}}$ signals in cerebellar granule neurons. These results indicate that low concentration of ethanol has diverse actions on the $Ca^{2+}}$ signals in cerebellar granule neurons.

• The Journal of Asian Finance, Economics and Business
• /
• 제9권3호
• /
• pp.1-10
• /
• 2022

This research proposes a novel trading method based on sample entropy for the FTSE China A50 Index. The approach is used to determine the points at which the index should be bought and sold for various holding durations. The findings are then compared to three other trading strategies: buying and holding the index for the entire time period, using the Relative Strength Index (RSI), and using the Moving Average Convergence Divergence (MACD) as buying/selling signaling tools. The unique entropy trading method, which used 90-day holding periods and was called StEn(90), produced the highest cumulative return: 25.66 percent. Regular buy and hold, RSI, and MACD were all outperformed by this strategy. In fact, when applied to the same time periods, RSI and MACD had negative returns for the FTSE China A50 Index. Regular purchase and hold yielded a 6% positive return, whereas RSI yielded a 28.56 percent negative return and MACD yielded a 33.33 percent negative return.

• 컴퓨터교육학회논문지
• /
• 제15권5호
• /
• pp.55-62
• /
• 2012

이동 통신 및 인터넷 기술의 발달과 보급화로 인하여 이동 기기(MH: Mobile Host)의 지속적이고 빠른 네트워크 연결은 필수적인 요소로 대두되고 있다. 일반적으로 무선 기기 사용자는 새로운 무선 영역에 진입할때 사용 가능한 여러 네트워크중 하나의 네트워크를 선택한다. 하지만 네트워크의 선택의 기준은 AP(Access Point)의 SSID(SubSystem IDentification)와 신호세기(Signal Strength)뿐이다. 이 두 가지 정보는 최적의 네트워크를 선택하기에는 충분한 정보는 되지 못한다. 따라서 최적의 AP의 선택을 위하여 더 많은 정보를 제공되어야 한다. 본 논문에서는 기존의 정보에 추가적으로 MH의 상태, 네트워크의 용량 및 부하, 그리고 네트워크 계층정보를 3계층 메시지인 라우터 광고 메시지(router advertisement message)를 사용하여 제공한다. 또한 이러한 정보를 기반으로 검색된 여러 AP중 자동적으로 최적의 AP를 선택하는 결정 엔진(DE: Decision Engine)을 제안한다. 시뮬레이션을 통하여 제안하는 DE을 사용함으로 MH측면에서는 무선 연결 시간 증가, 전원 소비 감소, 신호 부하 감소함을 증명하였다. 그리고 네트워크 측면에서는 AP와 라우터에서 부하 분산과 효율적인 네트워크 토폴로지가 제공됨을 증명하였다.

• 한국정보과학회논문지:정보통신
• /
• 제37권4호
• /
• pp.284-293
• /
• 2010

모바일 인터넷 환경에서 단말의 끊김 없는 서비스 제공을 위해 고속 핸드오버 기술이 필수적이다. 현재까지 다양한 핸드오버 기술들이 제안되었으며, Fast handover for Proxy Mobile IPv6(PFMIPv6)가 고속 핸드오버에 적용될 수 있는 유력한 기술이다. 본 논문에서는 PFMIPv6 기술이 모바일 와이맥스와 같은 무선 인터넷 환경에 효과적으로 적용될 수 있도록 예측 모델을 적용한 L2-L3 핸드오버의 크로스 레이어 기법을 제안하였다. 제안한 핸드오버 기법의 검증을 위해 맨하탄 모델, 고속도로 모델, 개방 모델의 세 가지 이동 환경을 고려하였으며, 각 환경에서 predictive-mode 동작 성공 확률, 핸드오버 지연시간, 패킷 손실량, 시그널링 비용에 대해서 비교 분석하였다. 성능 분석을 통해 제안한 방식이 PFMIPv6와 비교하여 더 우수한 성능을 나타냄을 확인하였다