• Asian Pacific Journal of Cancer Prevention
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• 제16권7호
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• pp.2813-2818
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• 2015

Objective: To investigate the regulatory effect of curcumin on expression of signal transducer and activator of transcription 3 (STAT3) in skin squamous cell carcinoma tissues as well as possible mechanisms of curcumin in prevention and treatment of skin squamous cell carcinoma. Materials and Methods: Highly invasive A431 cells were treated with curcumin at various doses .The cytotoxic effects of treatment with 5, 10, 15, 20, 25, 30, 35, 40 and 50 umol/L curcumin for 24, 48 and 72 hours on A431 cells were measured by MTT assay. The invasion capacity of cells treated with 5, 10 and 15 umol/L curcumin was measured by Transwell test, while adhesive ability was assessed by cell adhesion assay. The effects of 5,10 and 15 umol/L curcumin on expression levels of STAT3 were determined by Western blotting and on transcription levels of STAT3 mRNA by RT-PCR. Results: Treatment with curcumin at a doses of more than 15 umol/L for more than 24 hour inhibited the growth of A431 cells in a time-and dose-dependent fashion (p<0.001). The doses of 15 umol/L and less for 24 hours showed no significant cytotoxic effects on the cells, survival rates being more than 85%.The invasion and adhesive abilities decreased gradually with the increasing curcumin concentration, 15 umol/L exerting the strongest inhibitory effects (p<0.05). Curcumin showed significant dose-dependent inhibitory effects on the transcription level of STAT3 mRNA (p<0.05). Conclusions: Curcumin may reduce the invasive ability of A431 cells by inhibiting the activation of STAT3 signal pathway and expression of STAT3 as a target gene in the pathway.

• BMB Reports
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• 제52권9호
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• pp.572-576
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• 2019

Bisphosphonates are the mainstay of therapy worldwide for osteoporosis. However, bisphosphonates also have limitations. The objective of this study was to determine the role of miR-101-3p/Rap1b signal pathway in osteoclast differentiation after treatment with bisphosphonates. Our results revealed that miR-101-3p was an important regulator in bisphosphonates treated-osteoclasts. When miR-101-3p was down-regulated in bone marrow-derived macrophage-like cells (BMMs), the development of mature osteoclasts was promoted, and vice versa. However, alendronate decreased multinucleated cell number regardless of whether miR-101-3p was knocked down or over-expressed. TRAP activity assay confirmed the above results. Luciferase assay indicated that miR-101-3p was a negative regulator of Rap1b. Western blot analysis revealed that protein expression level of Rap1b in BMMs transfected with OV-miR-101-3p was lower than that in BMMs transfected with an empty vector. Rap1b overexpression increased TRAP-positive multinucleated cells, while Rap1b inhibition decreased the cell numbers. In vivo data showed that miR-101-3p inhibited osteoclast differentiation in ovariectomized mice while overexpressed of Rap1b blocked the differentiation. Taken together, our data demonstrate that miR-101-3p/Rap1b signal pathway plays a key role in osteoclast differentiation after treatment with bisphosphonates.

• 운동과학
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• 제26권3호
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• pp.229-237
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• 2017

PURPOSE: This study was to investigate the Glycolysis mediated sarcoplasmic reticulum (SR) $Ca^{2+}$ signal regulates mitochondria $Ca^{2+}$ during skeletal muscle contraction by using glycolysis inhibitor. METHODS: To examine the effect of Glycolysis inhibitor on SR and mitochondria $Ca^{2+}$ content, we used skeletal muscle fiber from gastrocnemius muscle. 2-deoxy glucose and 3-bromo pyruvate used as glycolysis inhibitor, it applied to electrically stimulated muscle contraction experiment. Intracellular $Ca^{2+}$ content, SR, mitochondria $Ca^{2+}$ level and mitochondria membrane potential (MMP) was detected by confocal microscope. Mitochondrial energy metabolism related enzyme, citric acid synthase activity also examined for mitochondrial function during the muscle contraction. RESULTS: Treatment of 2-DG and 3BP decreased the muscle contraction induced SR $Ca^{2+}$ increase however the mitochondria $Ca^{2+}$ level was increased by treatment of inhibitors and showed and overloading as compared with the control group. Glycolysis inhibitor and thapsigargin treatment showed a significant decrease in MPP of skeletal muscle cells compared to the control group. CS activity significantly decreased after pretreatment of glycolysis inhibitor during skeletal muscle contraction. These results suggest that regulation of mitochondrial $Ca^{2+}$ levels by glycolysis is an important factor in mitochondrial energy production during skeletal muscle contraction CONCLUSIONS: These results suggest that mitochondria $Ca^{2+}$ level can be regulated by SR $Ca^{2+}$ level and glycolytic regulation of intraocular $Ca^{2+}$ signal play pivotal role in regulation of mitochondria energy metabolism during the muscle contraction.

• 대한방사선치료학회지
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• 제22권1호
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• pp.25-32
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• 2010

목 적: 종양의 움직임을 고려하여 치료할 수 있는 RPM (Real time Position Management, Varian, USA) system을 사용하여 호흡을 고려한 방사선치료 시행 시, 제조사에서 제공하는 적외선 반사체는 일정각도 이상 couch가 회전할 경우 호흡신호 획득이 불가능하다. 이런 단점을 개선하고자 본 저자는 어느 각도에서나 호흡신호를 얻을 수 있는 3차원 적외선 반사체(3D infrared reflective marker)를 자체 개발하여 Kw-반사체라 명하고 그 유용성을 평가 하고자 한다. 대상 및 방법: 호흡신호의 안정성을 측정하기 위해 호흡운동을 재현 할 수 있는 3차원 구동팬텀(3D moving phantom) 위에 3가지 조건(A: couch 0도, 제조사의 반사체 B: couch 0도, Kw-반사체 C: couch 90도, Kw-반사체)의 적외선 반사체를 올려놓고 각각 3분 동안 호흡신호를 획득 하였다. 획득한 호흡신호는 호흡분석 프로그램(Labview ver 7.0)을 통해 최고값(peak value), 최저값(valley value), 표준편차(standard deviation), 변동값(variation value), 진폭값(amplitude value)을 획득하였다. 타겟의 회전 오차와 타겟의 이동범위를 알아보기 위해 3차원 구동팬텀 위에 B.B팬텀을 올려놓고 온-보드 영상장치(OBI)로 couch 0도와 90도 이미지를 획득한 후 B.B팬텀 중심에 위치한 ball bearing의 X, Y, Z값(mm)을 획득하였다. 결 과: 호흡신호를 분석한 결과 최고점에서 표준편차가 A: 0.002 B: 0.002 C: 0.003이고 진폭에 대한 호흡의 안정성은 A: 0.15% B: 0.14% C: 0.13%로 Kw반사체는 안정적으로 호흡신호를 얻을 수 있었다. couch 90도 회전 시 타겟인 ball bearing의 평균 회전오차는 X: -1.25 mm Y: -0.45 mm Z: +0.1 mm로 모든 방향에서 평균 1.3 mm 이내이고 타겟의 이동범위 차이도 평균 0.3 mm 이내였다. 결 론: couch가 회전하는 비동일면(Non-coplanar) 치료 시 Kw-반사체로 호흡신호를 얻은 결과 Kw-반사체는 안정적인 호흡신호를 획득할 수 있었고 제조사의 적외선반사체를 대체하기에 충분했다. 타겟의 회전오차와 이동범위 차이가 거의 없어 couch 회전 시 반사체 움직임의 변위가 호흡신호의 scale 및 진폭을 변화시킨 원인임을 알 수 있었다. 향후 couch angle값에 따른 진폭 높이의 변환 값을 연구해 적용한다면 비동일면에 대한 위상기반 gating 치료도 가능 할 것으로 사료된다.

• 한국지능시스템학회논문지
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• 제15권3호
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• pp.349-354
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• 2005

본 논문에서는 휴대용 개인정보 단말기를 이용하여 개인의 생체신호를 보다 쉽게 취득 하고 분석하는 시스템을 제안한다. 바쁘게 사는 현대인들이나, 거동이 불편한 환자들에게 병원을 직접 내원하여 검사를 받거나 진단을 받는 일은 큰 부담이 아닐 수 없다. 이에 본 연구에서는 원격지에서의 병원이나 타 의료장치에서 취득된 생체신호를 유비쿼터스 환경에서 블루투스로 전송하고 이 데이타를 블루투스가 내장된 PDA에서 획득하여 화면상에 다양한 표현방법으로 표현하는 생체신호 획득 시스템 구현을 목표로 한다. 이와 같은 시스템을 구현함으로서 이전의 생체신호와 현재의 생체신호를 쉽게 비교하고 분석할 수 있으며, 자신의 건강상태를 자가 점검하거나 의료기관의 의사에게 보내어짐으로서 검진 받을 수 있는 원격진료가 가능하다.

• 소음진동
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• 제9권3호
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• pp.577-586
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• 1999

Estimation of damping ratio for vibration signals measured on the passenger car's sear is useful for the objective evaluation of impact harshness in car. The vibratio signal is a transient signal represented by many coupled modes of suspension system. Wavelet transform automatically decouples these modes in the time-frequency domain. Damping ratios for decoupled modes are obtained by logarithmic treatment for the Wavelet transformed signal. The objective evaluation using Wavelet transform has been well corresponded with subjective evaluation done by skilled engineers.

• 비파괴검사학회지
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• 제32권4호
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• pp.355-361
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• 2012

This paper presents an experimental study on the detection and location of nonlinear scattering source due to the presence of fatigue crack in a laboratory specimen. The proposed technique is based on a combination of nonlinear elastic wave spectroscopy(NEWS) and time reversal(TR) focusing approach. In order to focus on the nonlinear scattering position due to the fatigue crack, we employed only one transmitting transducer and one receiving transducer, taking advantage of long duration of reception signal that includes multiple linear scattering such as mode conversion and boundary reflections. NEWS technique was then used as a pre-treatment of TR for spatial focusing of reemitted second harmonic signal. The robustness of this approach was demonstrated on a cracked specimen and the nonlinear TR focusing behavior is observed on the crack interface from which the second harmonic signal was originated.

• 대한의용생체공학회:의공학회지
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• 제6권1호
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• pp.5-12
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• 1985

In this paper Kalman filtering technique is applied to ultrasound signal to improve resolution capability, Ivhlch is in use of diagnostic imaging systems. The main advantage of Kalman filter algorithm for the analysis of reflected ultrasound signal is its recursive structure which can be easily adapted to tlme varing system. Because soft-tissues, such as liver, act as distributed acoustic low-pass filters which continually change the propagating pulse. tIne can put to practical use above advantage to find a suitable signal generallng model. In state-space description of the system, the 6th order system produces tl)e 1)esc spectral approximation to the source pulse As a result of spectrum analysis, 6th order estimator for two closely spaced ((p.5 mm) reflectors enhances resolution by 4dB-lOdB. By using this result, the possibility to detect even minute tumor is demonstrated.

• 한국정보통신학회:학술대회논문집
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• 한국해양정보통신학회 2002년도 춘계종합학술대회
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• pp.366-372
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• 2002

Tele-medicine and emergency medical system are necessary for moving from an accidental point or far distance to a hospital and emergency treatment or home treatment before a hospital. Emergency treatment is extremely important in the case of death before arriving a hospital and deformed or disabled by medical treatment delay. A necessary element for this medical system is the emergency communication system. This system is on preparing for an ability of furnishing patient status to a corresponding health service by monitoring the patient at an ambulance of the accident place. This is the transportation of basic biological information of a patient to a medical center by wireless communication system and the corresponding hospital or medical center examine the patient by monitoring, then they can send emergency medical order to the patient for emergency treatment. The TRS is most efficient way of emergency medical communication system, which is currently used with popularity. In this paper studied simultaneously a way of detecting and transporting bio-logical signals, and monitoring of transporting data with communication of voice in the accident place or ambulance.

• 한국항행학회논문지
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• 제21권6호
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• pp.651-658
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• 2017

방사선이 존재하는 환경에서 무색, 무미, 무촉의 방사선을 검출하기 위한 계측장비는 매우 중요하며 그동안 방사선 계측장비의 개발에 대한 많은 연구들이 있었다. 특히 방사선을 검출한 이후 측정된 검출신호를 손실 없이 효율적으로 처리하기 위해 검출된 미세전류를 전압형태로 정형하고, 이를 정확하게 판독하는 신호처리 부분은 매우 중요하다. 검출된 방사선 신호파형을 판독할 때, 지금까지는 신호의 전압파형을 짧은 시간 동안 일정한 값으로 유지시켜 파형의 크기를 판독한 후, 그 전기적인 값을 방전하고 다음 파형에 대비하는 피크홀드방식을 사용하였다. 이 연구에서는 방사선 검출기에서 측정된 검출신호 전압파형의 판독을 피크홀드방식이 아닌, 전압신호의 파고 정점에 이르는 시간을 포착하여 그 값을 직접 판독하는 방식을 제안한다. 이 방식에 의하면 전압 파고를 일정한 시간동안 유지하거나, 유지된 전압 파고를 초기상태로 만드는 복잡한 과정을 거치지 않고 검출된 방사선 신호를 정확하게 판독할 수 있다는 장점을 가지며, 실제 시뮬레이션을 통하여 이를 검증하였다.