• Investigative Magnetic Resonance Imaging
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• v.19 no.4
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• pp.212-217
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• 2015

Purpose: For a single time-point hyperpolarized $^{13}C$ magnetic resonance spectroscopy imaging (MRSI) of animal models, scan-time window after injecting substrates is critical in terms of signal-to-noise ratio (SNR) of downstream metabolites. Pre-scans of time-resolved magnetic resonance spectroscopy (MRS) can be performed to determine the scan-time window. In this study, based on two-site exchange model, protocol-specific simulation approaches were developed for $^{13}C$ MRSI and the optimal scan-time window was determined to maximize the SNR of downstream metabolites. Materials and Methods: The arterial input function and conversion rate constant from injected substrates (pyruvate) to downstream metabolite (lactate) were precalibrated, based on pre-scans of time-resolved MRS. MRSI was simulated using two-site exchange model with considerations of scan parameters of MRSI. Optimal scan-time window for mapping lactate was chosen from simulated lactate intensity maps. The performance was validated by multiple in vivo experiments of BALB/C nude mice with MDA-MB-231 breast tumor cells. As a comparison, MRSI were performed with other scan-time windows simply chosen from the lactate signal intensities of pre-scan time-resolved MRS. Results: The optimal scan timing for our animal models was determined by simulation, and was found to be 15 s after injection of the pyruvate. Compared to the simple approach, we observed that the lactate peak signal to noise ratio (PSNR) was increased by 230%. Conclusion: Optimal scan timing to measure downstream metabolites using hyperpolarized $^{13}C$ MRSI can be determined by the proposed protocol-specific simulation approaches.

• Journal of the Korean Society of Laryngology, Phoniatrics and Logopedics
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• v.18 no.1
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• pp.26-32
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• 2007

Objective and Background: Laryngeal stroboscope is an useful equipment in evaluation of vocal cord vibration and in early detection of mucosal lesion including invasive cancer of the vocal cord. Recently Lee et al. (2006) developed portable stroboscope using voice as synchronization signal. It has been frequently impaired ability to synchronize the flashes even in normal female. Authors tried to investigate various methods including vibration pick-up, microphone, laryngeal microphone, and contact microphone for development of simple and accurate method like electroglottograph signal. The purpose of this study was to estimate wheher the vibration pick-up is available and is consistent with the signal of EGG. Subjects and Methods: Authors compared the signals between EGG and noncontact method such as voice, contact methods including vibration pick-up, laryngeal microphone, and contact microphone in normal twenty adults (male 10 and female 10). The number of peak in one cycle was compared with the number of the peak in EGG, and the percent of phase difference in the peak was compared with EGG Also, authors tried to investigate which site of vibration pick-up was most effective for synchronization of stobo flashes. Three site including anterior neck below the cricoid cartilage, thyroid ala, and suprahyoid region were analysed. Results: Among various methods for synchronization of strobo flashes, vibration pick-up was most effective method in peak detection. And anterior neck below cricoid cartilage was the most available site of the vibration pick-up. Conclusion: Authors suggest that vibration pick-up is most available and effective method for synchronization of strobo flashes.

• Microbiology and Biotechnology Letters
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• v.26 no.6
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• pp.566-568
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• 1998

Partial nucleotide sequence (nt 1-1842) of chloramphenicol resistance plasmid pKH7 has been reported previously and residual nucleotide sequence (nt 1843-4118) of pKH7 was determined and then the complete nucleotide sequence of pKH7 was obtained. pKH7 consists of 4118 bp and has three ORFs. Besides Rep and CAT proteins described in previous paper, Pre protein which mediates site-specific recombination in Staphylococcus aureus was found to be on pKH7. R $S_{A}$, a site-specific recombination site of Pre protein, and palA, a specific lagging-strand conversion signal, was also found in pKH7. Amino acid sequence of Pre protein of pKH7 was compared with those of other antibiotic resistant Staphylococcus aureus plasmids.s.

• KSII Transactions on Internet and Information Systems (TIIS)
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• v.13 no.7
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• pp.3550-3565
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• 2019

This paper proposes a new measurement method for the effective measurement of the 99% occupied bandwidth (OBW) at monitoring stations. Although the OBW measurement of radio signal is recommended by the International Telecommunication Union Radio (ITU-R) with several methods, there still does not exist a clear measurement recommendation or standard for terrestrial DTV signal on-air environment. Modified adjacent channel power ratio (MACPR), which can be applied to 8-VSB (Vestigial Side Band) DTV (Digital Television) signal, is herein defined to verify the results of measurements obtained using the proposed measurement method. MACPR is a proper measuring parameter for determining the measuring area of a monitoring station. From measurement results obtained in real field environment, it has been found that the OBW of 8-VSB DTV signal can be effectively measured in areas where the MACPR value is over 35 dB and when the measurements are repeated more than 600 times in the same reception site. It also has been verified that measured results are within an error range of +/-0.1% compared to results directly obtained at a transmission station. It is expected that the proposed method is able to be employed in order to determine the proper location of monitoring station and provide a reliable OBW measurement procedure for 8-VSB DTV signal on-air environment.

• Proceedings of the Korea Committee for Ocean Resources and Engineering Conference
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• 2004.05a
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• pp.360-364
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• 2004

The objective of this study is to estimate the feasibility of acoustic emission method Jar the internal leak from the valves in nuclear power plants. The acoustic emission method was applied to the valves at the site, and the background noise was measured for the abnormal plant condition. From the comparison of background noise data with the experimental results as to relation between leak flow and acoustic signal, the minimum leak flow rates that am be detected by acoustic signal was suggested. When the background noise level are higher than the acoustic signal, the method described below was considered that the analysis the remainder among the background noise frequency spectrum and the acoustic signal spectrum.

• The Transactions of The Korean Institute of Electrical Engineers
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• v.61 no.2
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• pp.251-256
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• 2012

This study deals with the location of partial discharges(PDs) for power cables and GIS using 2 PD sensors and long coaxial signal cables. Throughout the experiments by use of simulated PD pulses and corona discharges in the power cable system, it is found out that the impedance matching between the coaxial signal cable and the measuring equipment should be done for good S/N ratio and the on-site measurement using more than 100m-long coaxial signal cable is possible. In addition, new PD location algorithm with the polarities of PD pulses and the arrival time difference between two pulses was proposed and was proved through the partial discharge experiments for the cable joint defect and artificial void defect.

• Proceedings of the KSME Conference
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• 2004.04a
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• pp.694-699
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• 2004

The purpose of this study is to estimate the availability of acoustic emission method to the internal leak of the valves at nuclear power plants. The acoustic emission method was applied to the valves at the site, and the background noise was measured for the abnormal plant condition. From the comparison of the background noise data with the experimental results as to relation between leak flow and acoustic signal, the minimum leak flow rates that can be detected by acoustic signal was suggested. When the background levels are higher than the acoustic signal, the method described below was considered that the analysis the remainder among the background noise frequency spectrum and the acoustic signal spectrum become a very useful leak detection method. A few experimental examples of the spectrum analysis that varied the background noise characteristic were given.

• Journal of Microbiology and Biotechnology
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• v.27 no.11
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• pp.1999-2009
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• 2017

The secretion efficiency of a protein in a Sec-type secretion system is mainly determined by an N-terminal signal peptide and its combination with its cognate protein. Five signal peptides, namely, two synthetic Sec-type and three Bacillus licheniformis alpha-amylase-derived signal peptides, were compared for periplasmic expression of the human growth hormone (hGH) in E. coli. Based on in silico predictions on the signal peptides' cleavage efficiencies and their corresponding mRNA secondary structures, a number of amino acid substitutions and silent mutations were considered in the modified signal sequences. The two synthetic signal peptides, specifically designed for hGH secretion in E. coli, differ in their N-terminal positively charged residues and hydrophobic region lengths. According to the mRNA secondary structure predictions, combinations of the protein and each of the five signal sequences could lead to different outcomes, especially when accessibility of the initiator ATG and ribosome binding sites were considered. In the experimental stage, the two synthetic signal peptides displayed complete processing and resulted in efficient secretion of the mature hGH in periplasmic regions, as was demonstrated by protein analysis. The three alpha-amylase-derived signal peptides, however, were processed partially from their precursors. Therefore, to achieve efficient secretion of a protein in a heterologous system, designing a specific signal peptide by using a combined approach of optimizations of the mRNA secondary structure and the signal peptide H-domain and cleavage site is recommended.

• Molecules and Cells
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• v.26 no.2
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• pp.212-215
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• 2008

The GC-rich discriminator sequence between the -10 region and the transcription start site of the rnpB promoter is responsible for stringent control of M1 RNA synthesis. The rnpB promoter also contains a G nucleotide at the previously identified transcription start site. In this study, we examined by mutagenesis of G to A whether this +1G nucleotide is involved in the stringent response. We found that the change did not alter the stringent response. Since the +1 mutation might alter transcription initiation, we compared the transcription start sites of the wt and mutant promoters by primer extension analysis. Surprisingly, we found that wild type rnpB transcription starts at both the +1G position (70%) and the -1C position (30%), and that the +1A mutation led to transcription initiation exclusively at the -1C position. We also generated two transversion mutations at the -1 position, both of which led to transcription starting exclusively at that position. The -1G mutant promoter gave a stringent signal similar to the wild-type, whereas the -1A mutant generated a significantly less stringent signal. Base on these results, we propose that a short sequence, up to 7 bp downstream of the -10 region, is involved in the stringent response of the rnpB promoter.

• Journal of Astronomy and Space Sciences
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• v.26 no.4
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• pp.659-666
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• 2009

CALVAL (Calibration & Validation) shall consider payloads characteristics because satellites have one and/or several payloads in order to perform their various missions. SAR satellite, one of various satellite, shall need to use special ground targets, which can reflect the radar signal to the satellite, because it can see objects with reflected radar signal. Therefore, the special ground targets, which are called generally reflector(corner reflector is the one of them) shall be installed and constructed on the ground path. The satellite must access the targets on that path. To accomplish successful calibration, the CALVAL site including corner reflectors will be surveyed and analyzed using various environment characteristics. In this paper, CALVAL site including point targets(corner reflector) for absolute radiometric calibration except one including distributed targets for relative radiometric calibration has been deeply considered.