• Journal of Microbiology and Biotechnology
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• v.30 no.2
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• pp.196-205
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• 2020

In this study, the acidic lipase from Aspergillus niger (ANL) was homologously expressed in A. niger. The expression of ANL was significantly improved by the expression of the native ANL with the introns, the addition of the Kozak sequence and the optimization of the signal sequences. When the cDNA sequence of ANL fused with the glaA signal was expressed under the gpdA promoter in A. niger, no lipase activity could be detected. We then tried to improve the expression by using the full-length ANL gene containing three introns, and the lipase activity in the supernatant reached 75.80 U/ml, probably as a result of a more stable mRNA structure. The expression was further improved to 100.60 U/ml by introducing a Kozak sequence around the start codon due to a higher translation efficiency. Finally, the effects of three signal sequences including the cbhI signal, the ANL signal and the glaA signal on the lipase expression were evaluated. The transformant with the cbhI signal showed the highest lipase activity (314.67 U/ml), which was 1.90-fold and 3.13-fold higher than those with the ANL signal and the glaA signal, respectively. The acidic lipase was characterized and its highest activity was detected at pH 3.0 and a temperature of 45℃. These results provided promising strategies for the production of the acidic lipase from A. niger.

• Journal of Microbiology and Biotechnology
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• v.2 no.1
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• pp.56-65
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• 1992

The complete nucleotide sequence of the Bacillus circulans F-2 RSDA gene, coding for raw starch digesting a-amylase (RSDA), has been determined. The RSDA structure gene consists of an open reading frame of 2508 bp. Six bp upstream of the translational start codon of the RSDA is a typical gram-positive Shine-Dalgarno sequence and the RSDA encodes a preprotein of 836 amino acids with an Mr of 96, 727. The gene was expressed from its own regulatory region in E. coli and two putative consensus promoter sequences were identified upstream of a ribosome binding site and an ATG start codon. Confirmation of the nucleotide sequence was obtained and the signal peptide cleavage site was identified by comparing the predicted amino acid sequence with that derived by N-terminal analysis of the purified RSDA. The deduced N-terminal region of the RSDA conforms to the general pattern for the signal peptides of secreted prokaryotic proteins. The complete amino acid sequence was deduced and homology with other enzymes was compared. The results suggested that the Thr-Ser-rich hinge region and the non-catalytic domain are necessary for efficient adsorption onto raw substrates, and the catalytic domain (60 kDa) is necessary for the hydrolysis of substrates, as suggested in previous studies (8, 9).

• Proceedings of the KIEE Conference
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• 1993.07a
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• pp.447-449
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• 1993

Digital filter is a signal processor which converts the sequence input sampled from analog signal into another sequence output. It includes software routines which filter digital signal, a computer system for executing the routines, and a data acquisition system which implements A/D, D/A signal conversion. In this paper, a data acquisition system is designed and implemented for one-board computer of MC68000. Also, the theory regarding signal conversion and and its problems occured in implementation are considered. And then, with the hardware implemented like this, design of a digital low-pass filter with the cutoff frequency of 200Hz is implemented, and related characteristics are considered.

• The Journal of Korean Institute of Communications and Information Sciences
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• v.39A no.11
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• pp.637-644
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• 2014

This paper investigates the detection performance of fault location using STDR(sequence time domain reflectometry) and SSTDR(spread spectrum time domain reflectometry) with various length and types of sequences, and then, proposes an improved detection technique by eliminating the injected signal in SSTDR. The detection error rates are compared and analyzed in power line channel model with various fault locations, fault types, and spreading sequences such as m-sequence, binary Barker sequence, and 4-phase Frank sequence. It is shown that the proposed technique is able to improve the detection performance obviously when the reflected signal is weak or the fault location is extremely close.

• The Journal of Korean Institute of Communications and Information Sciences
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• v.24 no.6B
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• pp.1015-1023
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• 1999

We propose nonlinear processing schemes for robust acquisition of direct-sequence spread spectrum (DS/SS) signals in wideband pulse jamming. To mitigate the interference effect due to impulse-like wideband jamming signals, the received signal is preprocessed by using the median filter, a simple order statistic filter Since only parts of the PN sequence are used for rapid acquisition, it is indispensable for analytic design of an acquisition scheme to have an appropriate model of the partial PN signal. The partial correlation of the median filtered PN signal is approximated by a two-piecewise linear model using an approximate upper bound. The acquisition performance of the proposed schemes is compared to that of other schemes. Finally, the analytic design is verified by computer simulation.

• Journal of Magnetics
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• v.21 no.1
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• pp.148-152
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• 2016

The main goal was to evaluate effectiveness of a modified TSE sequence compared with DIR (double inversion recovery) sequence in acquisition of fast flow brain vessel images using signal void effect. 32 healthy volunteers (10 men and 22 women; mean age of 31 years; ranging between 28-43 years) who underwent black blood DIR sequence (group A) and the modified TSE sequence (group B) were enrolled in our study. Signal to Noise Ratio (SNR) and Contrast to Noise Ratio (CNR) of the internal carotid arteries' lumen were compared in T1 and T2 weighted images for both group A and B. The images obtained from group B showed lower SNR values in internal carotid artery than the group A in both of the T1 and T2 weighted images (11.49% and 13.66% respectively). While the CNR values were higher in the group B than the group A in both of the T1 and T2 weighted images (8.69% and 7.55 % respectively). The qualitative score of all categories were not significantly different between the two groups. Furthermore approximately 49% of the total scan time was reduced from group B. Our study is to shorten the scanning time and minimize the inconveniences of the patients in acquisition of the black blood images of brain by using the signal void effect in the modified TSE technique while keeping the diagnostic value of the test.

• Investigative Magnetic Resonance Imaging
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• v.3 no.1
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• pp.13-19
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• 1999

MRI imaging provides many benefits such as noninvasive, 3-dimensional imaging capabilities. But it has relatively serious drawback that is the long data collection time, compared with other imaging modality. Many studies have been performed for fast MR imaging. But EPI and SEPI (4-6) are required to expensive hardware. In this paper, we introduce to Burst imaging technique. It can reduce imaging time by use of a mulitple RF excitation technique. Further it is easily implemented to the normal MRI system. But a pixel profile in the conventional burst sequence is so poor that excited area by burst sequence is a small portion of a pixel. This causes poor signal to noise ratio in burst image. therefore frequency sweeping of RF pulse for burst imaging sequence is proposed to improve pixel profile. A burst pulse train is shaped by liner or nonlinear frequency sweeping function so that all the spins within a pixel are excited, thereby improving the signal to noise ratio. It also shows that the pixel profiles are dependent on how frequency sweep is made. Computer simulations with Bloch equation and experimental results obtained using a 1.0 T NMR imaging system are presented.

• Journal of the Korean Institute of Telematics and Electronics
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• v.21 no.6
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• pp.105-114
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• 1984

A fast pseudo-noise (PN) sequence acquisition algorithm for the direct-sequence (DS) spread spectrum system is proposed. The basic concept of the algorithm has been adopted from that of the classical sliding correlator. Mathematical modeling, analysis and computer simulation of the proposed system have been done. The results of analysis and computer simulation show that the acquisition system yields a significant performance improvement over the sliding correlator. Its acquisition time takes only 45 ms when signal-to-noise ratio(SNR) is -18dB. The algorithm developed has been implemented in hardware and its experimental result is also given.

• The Transactions of The Korean Institute of Electrical Engineers
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• v.58 no.5
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• pp.1041-1046
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• 2009

In this paper, we proposed a robust watermark scheme for image based on SVD(Singular Value Transform) and Triplet. First, the original image is decomposed by using 3-level DWT, and then used the singular values changed for embedding and extracting of the watermark sequence in LL3 band. Since the matrix of singular values is not easily altered with various signal processing noises, the embedded watermark sequence has the ability to withstand various signal processing noise attacks. Nevertheless, this method does not guarantee geometric transformation(such as rotation, cropping, etc.) because the geometric transformation changes the matrix size. In this case, the watermark sequence cannot be extracted. To compensate for the above weaknesses, a method which uses the triplet for embedding a barcode image watermark in the middle of frequency band is proposed. In order to generate the barcode image watermark, the pattern of the watermark sequence embedded in a LL3 band is used. According to this method, the watermark information can be extracted from attacked images.

• Proceedings of the Korean Society of Medical Physics Conference
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• 2002.09a
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• pp.341-344
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• 2002

Neurodegenerative disorders, like Alzheimer's disease, are often accompanied by reduced brain perfusion (cerebral blood flow). Using the intrinsic magnetic properties of water, arterial spin labeling magnetic resonance imaging (ASLMRI) can map brain perfusion without injection of radioactive tracers or contrast agents. However, accuracy in measuring perfusion with ASL-MRI can be limited because of contributions to the signal from stationary spins and because of signal modulations due to transient magnetic field effects. The goal was to optimize ASL-MRI for perfusion measurements in the aging human brain, including brains with Alzheimer's disease. A new ASL-MRI sequence was designed and evaluated on phantom and humans. Image texture analysis was performed to test quantitatively improvements. Compared to other ASL-MRI methods, the newly designed sequence provided improved signal to noise ratio improved signal uniformity across slices, and thus, increased measurement reliability. This new ASL-MRI sequence should therefore provide improved measurements of regional changes of brain perfusion in normal aging and neurodegenerative disorders.