• Journal of Microbiology and Biotechnology
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• v.9 no.1
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• pp.113-118
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• 1999

The insulin-like growth factor (IGF) is found in all vertebrates and its type-II molecule is regarded as a fundamental embryonic growth factor during development. We have firstly identified, in this study, a cDNA clone corresponding to IGF-II (flIGF-II) from the adult brain of the teleost, Paralichthys olivaceus. We also examined the tissue expression of flIGF-II in several adult tissues by RT-PCR. The flIGF-II cDNA contained a complete ORF consisting of 215 amino acids and one stop codon. Its molecular characteristics appear to be similar to the previously identified IGF-II molecules, in which a common primary structure exhibiting B, C, A, D, and E domains is evidently observed. This cDNA clone seems to be cleaved at $Ala_{52}$ for the $NH_2$-end signal peptide and appears to produce a 98 amino acid-long E-peptide from the $Arg^{118}$. The functional B-D domain regions, therefore, include 65 amino acids and is able to encode a 7.4-kDa protein. The most prominent structural difference between IGF-I and IGF-II was that the D domain of IGF-II exhibits a two-codon-deleted pattern compared to the 8 amino acid-containing IGF-I. The insulin family signature in the A domain and six cysteins forming three disulfide bridges between the B and A domains were evolutionary-conserved from teleosts to mammalian IGF-II. Interestingly, the E-peptide region appears to provide a distinct hallmark between teleosts in amino acid composition. The flIGF-II shows 85.1% of sequence identity to salmon and trout, 90.6% to tilapia, and 98.4% to perch in amino acid level. In tissue expressions of IGF-II, it is very likely that flIGF-II has a significant expression in the adult brain. However, liver seems to be the main source for IGF-II production, and relatively low signals were observed in the adult muscle and kidney. Taken together, it would be concluded that the functional region for IGF-II mRNA is highly similar in phylogeny and is evolutionary, conserved as a mediator for the growth of vertebrates.

• Journal of Biomedical Engineering Research
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• v.28 no.5
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• pp.676-683
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• 2007

A non-invasive respiratory gated radiotherapy system like those based on external anatomic motion gives better comfortableness to patients than invasive system on treatment. However, higher correlation between the external and internal anatomic motion is required to increase the effectiveness of non-invasive respiratory gated radiotherapy. Both of invasive and non-invasive methods need to track the internal anatomy with the higher precision and rapid response. Especially, the non-invasive method has more difficulty to track the target position successively because of using only image processing. So we developed the system to track the motion for a non-invasive respiratory gated system to accurately find the dynamic position of internal structures such as the diaphragm and tumor. The respiratory organ motion tracking apparatus consists of an image capture board, a fluoroscopy system and a processing computer. After the image board grabs the motion of internal anatomy through the fluoroscopy system, the computer acquires the organ motion tracking data by image processing without any additional physical markers. The patients breathe freely without any forced breath control and coaching, when this experiment was performed. The developed pattern-recognition software could extract the target motion signal in real-time from the acquired fluoroscopic images. The range of mean deviations between the real and acquired target positions was measured for some sample structures in an anatomical model phantom. The mean and max deviation between the real and acquired positions were less than 1mm and 2mm respectively with the standardized movement using a moving stage and an anatomical model phantom. Under the real human body, the mean and maximum distance of the peak to trough was measured 23.5mm and 55.1mm respectively for 13 patients' diaphragm motion. The acquired respiration profile showed that human expiration period was longer than the inspiration period. The above results could be applied to respiratory-gated radiotherapy.

• Journal of Satellite, Information and Communications
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• v.10 no.2
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• pp.109-114
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• 2015

Frame synchronization is achieved by correlation between received symbols and a preamble pattern which is periodically appended at a frame header. In this paper, we deal with a frame detection method complaint with satellite-based DVB-S2 system. In DVB-S2, frame synchronization is performed under the low signal-to-noise ratio(SNR), a large frequency offset which can be up to 20% of a symbol transmission rate and unknown modulation schemes ranging from QPSK to 32-APSK. In this environment, we propose a method combining differential correlation based on SOF and PLSC with an accumulated correlation method for the detection of frame structures. In addition, detection performances about mean acquisition time(MAT) and detection error probability are evaluated via computer simulations.

• Journal of Korea Multimedia Society
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• v.15 no.11
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• pp.1305-1318
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• 2012

Image segmentation is one of the difficult research problems in machine vision and pattern recognition field. A commonly used segmentation method is the Otsu method. It is simpler and easier to implement but it fails if the histogram is unimodal or similar to unimodal. And if some target area is smaller than background object, then its histogram has the distribution close to unimodal. In this paper, we proposed an improved image segmentation method based on 1D Otsu method for a small target detection. To overcome drawbacks by unimodal histogram effect, we depressed the background histogram using a logarithm function. And to improve a signal to noise ratio, we used a local average value by the neighbor window for thresholding using 1D Otsu method. The experimental results show that our proposed algorithm performs better segmentation result than a traditional 1D Otsu method, and needs much less computational time than that of the 2D Otsu method.

• The Korean Journal of Physiology and Pharmacology
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• v.7 no.2
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• pp.97-101
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• 2003

The salivary glands produce 1.5L of fluid per day. As in other exocrine organs, the general mechanism in the salivary glands is that water movement occurs secondary to osmotic driving forces created by active salt transport. Therefore, high water permeability in the salivary glands is expected to have a variety of aquaporin (AQP), a water channel. Although some AQPs have been known to be present in the salivary glands, roles of parasympathetic nerve in AQP expression have not yet been examined. This study was designed to examine the changes of AQPs and extracellular signal-regulated kinase (ERK) in the submandibular glands after parasympathetic denervation. Right chorda-lingual nerve was cut, and each right (experiment) and left (control) submandibular gland was excised at 1, 3, 7, 14, 30 days after denervation. The denervated right submandibular glands were resulted in weight loss and morphologic changes, including cell loss and atrophy, as the time elapsed after parasympathetic denervation increased, whereas there were no histologic alteration in control side. AQP5 which is known to reside in apical membrane and secretory caraliculi of the submandibular acini were gradually underexpressed according, as the time after denervation increased. Expression of AQP4 in submandibular ductal epithelium was down-regulated after denervation. Besides, AQP3 and 8, which is known to be present in basolateral membrane of the glandular acini, were gradually underexpressed after denervation, similar to the pattern of other types. Expression of ERK, a mitogen-activated protein kinase, was downregulated after parasympathetic denervation in the submandibular gland. These results suggest that parasympathetic nervous system regulates the expression of AQPs in salivary glands, and is in part mediated by ERK pathway.

• The Korean Journal of Physiology and Pharmacology
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• v.15 no.6
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• pp.363-369
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• 2011

The present study elucidated the effect of the selective inducible nitric oxide synthase (iNOS) inhibitor $N^6$-(1-iminoethyl)-L-lysine (L-NIL) on monosodium urate (MSU) crystal-induced inflammation and edema in mice feet. L-NIL (5 or 10 mg/kg/day) was administered intraperitoneally 4 h before injection of MSU (4 mg) into the soles of mice hindlimb feet. Twenty-four hours after MSU injection, foot thickness was increased by 160% and L-NIL pretreatment reduced food pad swelling in a dose dependent manner. Pretreatment of 10 mg/kg/day L-NIL significantly suppressed the foot pad swelling by MSU. Plasma level of nitric oxide (NO) metabolites and gene expression and protein level of iNOS in feet were increased by MSU, which was suppressed by L-NIL pretreatment. Similar pattern of change was observed in nitrotyrosine level. MSU increased the gene expression of tumor necrosis factor (TNF)-${\alpha}$ and interleukin (IL)-$1{\beta}$ and L-NIL pretreatment suppressed MSU-induced cytokines expression. The mRNA levels of superoxide dismutase and glutathione peroxidase1 were increased by MSU and L-NIL pretreatment normalized the gene expression. Phosphorylation of extracellular signal-regulated kinase 1/2 and p38 was increased by MSU, which was suppressed by L-NIL pretreatment. The mRNA levels of iNOS, TNF-${\alpha}$, and IL-$1{\beta}$ were increased by MSU in human dermal fibroblasts, C2C12 myoblasts, and human fetal osteoblasts in vitro, which was attenuated by L-NIL in a dose dependent manner. This study shows that L-NIL inhibits MSU-induced inflammation and edema in mice feet suggesting that iNOS might be involved in MSU-induced inflammation.

• The Journal of the Society of Korean Medicine Diagnostics
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• v.19 no.2
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• pp.91-100
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• 2015

Objectives The aim of this study is to develop a structured clinical protocol related with acquisition of radial pulse wave in the randomized, $2{\times}2$ cross-over design, and cold-heat intervention trial for a pilot and preliminary study. Methods The protocol was contrived based on wide ranging literature searches for cold-heat intervention experiments and radial pulse diagnoses. Results Sample size of 60 subjects was calculated based on an effect size derived from the previous study designed to detect the pre-post cold-heat differences in the radial pulse. Each subjects will be randomly assigned to the cold (first) to heat (last) group (n=30) or heat (first) to cold (last) group (n=30). All subjects will fill out a case report form and questionnaires related with pattern identification, dietary patterns, sleep quality, and physical activity will be surveyed and used as a secondary outcomes. Safety assessment will be reported at the final stage. Conclusions This protocol will provide an additional reference to future studies related with observation of radial pulse during any interventions and also expect to be used as a guideline for acquisition of reliable radial pulse wave data.

• The Journal of Korean Institute of Communications and Information Sciences
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• v.31 no.1C
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• pp.36-45
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• 2006

In this paper, multiview calibration system for an effective 3D display is proposed. This system can be obtain 4-view image from multiview camera system. Also it can be rectify lens and camera distortion, error of bright and color, and it can be calibrate distortion of geometry. In this paper, we proposed the signal processing skill to calibrate the camera distortions which are able to take place from the acquisited multiview images. The discordance of the brightness and the colors are calibrated the color transform by extracting the feature point, correspondence point. And the difference of brightness is calibrated by using the differential map of brightness from each camera image. A spherical lens distortion is corrected by extracting the pattern of the multiview camera images. Finally the camera error and size among the multiview cameras is calibrated by removing the distortion. Accordingly, this proposed rectification & calibration system enable to effective 3D display and acquire natural multiview 3D image.

• 제어로봇시스템학회:학술대회논문집
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• 2005.06a
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• pp.1439-1444
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• 2005

Induction motors are the most commonly used electrical drives because they are rugged, mechanically simple, adaptable to widely different operating conditions, and simple to control. The most common faults in squirrel-cage induction motors are bearing, stator and rotor faults. Surveys conducted by the IEEE and EPRI show that the most common fault in induction motor is bearing failure (${\sim}$40% of failure). Thence, this paper addresses experimental results for diagnosing faults with different rolling element bearing damage via motor current spectral analysis. Rolling element bearings generally consist of two rings, an inner and outer, between which a set of balls or rollers rotate in raceways. We set the experimental test bed to detect the rolling-element bearing misalignment of 3 type induction motors with normal condition bearing system, shaft deflection system by external force and a hole drilled through the outer race of the shaft end bearing of the four pole test motor. This paper takes the initial step of investigating the efficacy of current monitoring for bearing fault detection by incipient bearing failure. The failure modes are reviewed and the characteristics of bearing frequency associated with the physical construction of the bearings are defined. The effects on the stator current spectrum are described and related frequencies are also determined. This is an important result in the formulation of a fault detection scheme that monitors the stator currents. We utilized the FFT, Wavelet analysis and averaging signal pattern by inner product tool to analyze stator current components. The test results clearly illustrate that the stator signature can be used to identify the presence of a bearing fault.

• Journal of the Microelectronics and Packaging Society
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• v.27 no.3
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• pp.61-67
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• 2020

In this paper, we calculated the warpage of bare substrates and chip attached substrates by using FEM (Finite Element Method), and compared and analyzed the effect of the chips' attachment on warpage. Also, the effects of layer thickness of substrates for reducing warpage were analyzed and the conditions of layer thickness were analyzed by signal-to-noise ratio of Taguchi method. According to the analysis results, the direction of warpage pattern in substrates can change when chips are attached. Also, the warpage decreases as the difference in the CTE (coefficient of thermal expansion) between the top and bottom of the package decreases and the stiffness of the package increases after chips are loaded. In addition, according to the impact analysis of design parameters on substrates where chips are not attached, in order to reduce warpage, the inner layers of the circuit layer Cu1 and Cu4 has be controlled first, and then concentrated on the thickness of the solder resist on the bottom side and the thickness of the prepreg layer between Cu1 and Cu2.