• Title/Summary/Keyword: Signal Optimization

Search Result 751, Processing Time 0.024 seconds

Optimization for Production of Exo-β-1,3-glucanase (Laminarinase) from Aspergillus oryzae in Saccharomyces cerevisiae (Saccharomyces cerevisiae에서 Aspergillus oryzae 유래의 exo-β-1,3-glucanase (laminarinase)의 생산 최적화)

  • Kim, Min-Jung;Nam, Soo-Wan;Tamano, Koichi;Machida, Masayuki;Kim, Sung-Koo;Kim, Yeon-Hee
    • KSBB Journal
    • /
    • v.26 no.5
    • /
    • pp.427-432
    • /
    • 2011
  • In this study, a EXGA gene code for exo-β-1,3-glucanase from Aspergillus oryzae was overexpressed and secretory produced in Saccharomyces cerevisiae. To overexpress the β-1,3-glucanase, pGInu-exgA and pAInu-exgA plasmids having GAL10 and ADH1 promoter, respectively, and exoinulinase signal sequence (Inu s.s) were constructed and introduced in S. cerevisiae SEY2102 and 2805. The recombinant β-1,3-glucanase was successfully expressed and secreted into the medium and the β--1,3-glucanase activity in 2102/pGInu-exgA and 2102/pAInu-exgA strain were 5.01 unit/mL and 4.09 unit/mL, respectively. In the 2805/pGInu-exgA and 2805/pAInu-exgA strain, the β-1,3-glucanase activity showed 3.23 unit/mL and 3.22 unit/mL, respectively. Secretory efficiency in each strain reached 95% to 98%. Subsequently, the recombinant β1,3-glucanase was used for ethanol production. Ethanol productivity in 2102/pAInu-exgA strain was 0.83 g/L when pre-treated Laminaria japonica which has initial reducing sugar of 1.4 g/L was used as substrate. It is assumed that the polysaccharides of Laminaria japonica was effectively saccharified by recombinant β-1,3-glucanase, resulting in increase of ethanol productivity. These results suggested that recombinant β-1,3-glucanase was efficiently overexpressed and secreted in S. cerevisiae SEY2102 as host strain by using ADH1 promoter-Inu s.s system.

Pulse Shape Design for Ultra-Wideband Radios Using Projections onto Convex Sets (POCS를 이용한 초광대역 무선통신의 펄스파형 설계)

  • Lee, Seo-Young
    • The Journal of Korean Institute of Communications and Information Sciences
    • /
    • v.33 no.3A
    • /
    • pp.311-318
    • /
    • 2008
  • We propose new pulse shapes for FCC-compliant ultra-wideband (UWB) radios. The projections onto convex sets (POCS) technique is used to optimize temporal and spectral shapes of UWB pulses under the constraints of all of the desired UWB signal properties: efficient spectral utilization under the FCC spectral mask, time-limitedness, and good autocorrelation. Simulation results show that for all values of the pulse duration, the new pulse shapes not only meet the FCC spectral mask most efficiently, but also have nearly the same autocorrelation functions. It is also observed that our truncated (i.e., strictly time-limited) pulse shapes outperform the truncated Gaussian monocycle in the BER performance of binary TH-PPM systems for the same pulse durations. The POCS technique provides an effective method for designing UWB pulse shapes in terms of its inherent design flexibility and joint optimization capability.

User Scheduling Algorithm for Cell Capacity Maximization in Full Duplexing Wireless Cellular Networks (전이중 무선 셀룰라 네트워크에서 셀 용량 최대화를 위한 사용자 스케쥴링 방식)

  • Choi, Hyun-Ho
    • Journal of the Korea Institute of Information and Communication Engineering
    • /
    • v.18 no.11
    • /
    • pp.2613-2620
    • /
    • 2014
  • In this paper, we consider a full duplexing (FD) wireless cellular network where a central base station (BS) works in the FD mode while the downlink (DL) and uplink (UL) users work in the time division duplexing (TDD) mode. Since this FD system induces the inter-user interference from UL user to DL user, the main challenge for maximizing the system performances is user scheduling that makes a pair of DL user and UL user to use the same radio resource simultaneously. We formulate an optimization problem for user pairing to maximize the cell capacity and propose a suboptimal user scheduling algorithm with low complexity. This scheduling algorithm is designed in a way where the DL user with a better signal quality has a higher priority to choose its UL user that causes less interference. Simulation results show that the FD system using the proposed user scheduling algorithm achieves the optimal performance and significantly outperforms the conventional TDD system in terms of the cell capacity.

BGA Deveop Process Optimization for the Vertical Wet Equipment Using Taguchi Experiment (다구찌 방법을 이용한 BGA 현상 공정용 수직 습식 장비의 공정 최적화)

  • Ryu, Sun-Joong
    • Journal of the Korean Vacuum Society
    • /
    • v.18 no.4
    • /
    • pp.310-317
    • /
    • 2009
  • Vertical wet equipment for the BGA develop process was newly developed substituted for conventional horizontal wet equipment. The benefits of vertical equipment are that the pattern damages generated by the collision between the patterns and transferring rollers can be eliminated because the direct contact between the equipment's transferring units and the soft dry film patterns does not occurs. Taguchi experiment was conducted to optimize the process characteristics for the vertical equipment. The experiment was organized as the smaller the better problem which includes adequate uncontrollable factor and controllable factors. The uncontrollable factors are the 4 sides of two panels which are loaded to the equipment at the same time. By the analysis of the experiment, temperature of the develop chemicals and develop spraying time are analyzed as the main controllable factors. Finally, line pattern's minimum width which is not damaged for the develop process was improved from $13.8{\mu}m$ for the horizontal equipment to $10.4{\mu}m$ for the vertical equipment. And dot pattern's minimum width is improved from $22.1{\mu}m$ to $16.3{\mu}m$.

A Novel Ambiguity Resolution Method of Radar Pulses using Genetic Algorithm (유전 알고리즘 기반 레이더 펄스 모호성 해결방법)

  • Han, Jinwoo;Jo, Jeil;Kim, Sanhae;Park, Jintae;Song, Kyuha
    • Journal of the Institute of Electronics and Information Engineers
    • /
    • v.52 no.4
    • /
    • pp.184-193
    • /
    • 2015
  • Passive Surveillance System based on the TDOA detects the emitter position in the air using TOA of pulses comprising emitter signal from multiple receivers. In case that PRI of pulses from the emitter is not enough big in comparison with the distance among receivers, it causes the ambiguity problem in selecting proper pulse pairs for TDOA emitter geolocation. In this paper, a novel ambiguity resolution method of radar pulses is presented by using genetic algorithm after changing ambiguity problem into optimization problem between TDOA of received pulses from each receiver and emitter position. Simulation results are presented to show the performance of the proposed method.

Development of Strain-gauge-type Rotational Tool Dynamometer and Verification of 3-axis Static Load (스트레인게이지 타입 회전형 공구동력계 개발과 3축 정적 하중 검증)

  • Lee, Dong-Seop;Kim, In-Su;Lee, Se-Han;Wang, Duck-Hyun
    • Journal of the Korean Society of Manufacturing Process Engineers
    • /
    • v.18 no.9
    • /
    • pp.72-80
    • /
    • 2019
  • In this task, the tool dynamometer design and manufacture, and the Ansys S/W structural analysis program for tool attachment that satisfies the cutting force measurement requirements of the tool dynamometer system are used to determine the cutting force generated by metal cutting using 3-axis static structural analysis and the LabVIEW system. The cutting power in a cutting process using a milling tool for processing metals provides useful information for understanding the processing, optimization, tool status monitoring, and tool design. Thus, various methods of measuring cutting power have been proposed. The device consists of a strain-gauge-based sensor fitted to a new design force sensing element, which is then placed in a force reduction. The force-sensing element is designed as a symmetrical cross beam with four arms of a rectangular parallel line. Furthermore, data duplication is eliminated by the appropriate setting the strain gauge attachment position and the construction of a suitable Wheatstone full-bridge circuit. This device is intended for use with rotating spindles such as milling tools. Verification and machining tests were performed to determine the static and dynamic characteristics of the tool dynamometer. The verification tests were performed by analyzing the difference between strain data measured by weight and that derived by theoretical calculations. Processing test was performed by attaching a tool dynamometer to the MCT to analyze data generated by the measuring equipment during machining. To maintain high productivity and precision, the system monitors and suppresses process disturbances such as chatter vibration, imbalances, overload, collision, forced vibration due to tool failure, and excessive tool wear; additionally, a tool dynamometer with a high signal-to-noise ratio is provided.

Design, calibration and application of wireless sensors for structural global and local monitoring of civil infrastructures

  • Yu, Yan;Ou, Jinping;Li, Hui
    • Smart Structures and Systems
    • /
    • v.6 no.5_6
    • /
    • pp.641-659
    • /
    • 2010
  • Structural Health Monitoring (SHM) gradually becomes a technique for ensuring the health and safety of civil infrastructures and is also an important approach for the research of the damage accumulation and disaster evolving characteristics of civil infrastructures. It is attracting prodigious research interests and the active development interests of scientists and engineers because a great number of civil infrastructures are planned and built every year in mainland China. In a SHM system the sheer number of accompanying wires, fiber optic cables, and other physical transmission medium is usually prohibitive, particularly for such structures as offshore platforms and long-span structures. Fortunately, with recent advances in technologies in sensing, wireless communication, and micro electro mechanical systems (MEMS), wireless sensor technique has been developing rapidly and is being used gradually in the SHM of civil engineering structures. In this paper, some recent advances in the research, development, and implementation of wireless sensors for the SHM of civil infrastructures in mainland China, especially in Dalian University of Technology (DUT) and Harbin Institute of Technology (HIT), are introduced. Firstly, a kind of wireless digital acceleration sensors for structural global monitoring is designed and validated in an offshore structure model. Secondly, wireless inclination sensor systems based on Frequency-hopping techniques are developed and applied successfully to swing monitoring of large-scale hook structures. Thirdly, wireless acquisition systems integrating with different sensing materials, such as Polyvinylidene Fluoride(PVDF), strain gauge, piezoresistive stress/strain sensors fabricated by using the nickel powder-filled cement-based composite, are proposed for structural local monitoring, and validating the characteristics of the above materials. Finally, solutions to the key problem of finite energy for wireless sensors networks are discussed, with future works also being introduced, for example, the wireless sensor networks powered by corrosion signal for corrosion monitoring and rapid diagnosis for large structures.

A Survey of Genetic Programming and Its Applications

  • Ahvanooey, Milad Taleby;Li, Qianmu;Wu, Ming;Wang, Shuo
    • KSII Transactions on Internet and Information Systems (TIIS)
    • /
    • v.13 no.4
    • /
    • pp.1765-1794
    • /
    • 2019
  • Genetic Programming (GP) is an intelligence technique whereby computer programs are encoded as a set of genes which are evolved utilizing a Genetic Algorithm (GA). In other words, the GP employs novel optimization techniques to modify computer programs; imitating the way humans develop programs by progressively re-writing them for solving problems automatically. Trial programs are frequently altered in the search for obtaining superior solutions due to the base is GA. These are evolutionary search techniques inspired by biological evolution such as mutation, reproduction, natural selection, recombination, and survival of the fittest. The power of GAs is being represented by an advancing range of applications; vector processing, quantum computing, VLSI circuit layout, and so on. But one of the most significant uses of GAs is the automatic generation of programs. Technically, the GP solves problems automatically without having to tell the computer specifically how to process it. To meet this requirement, the GP utilizes GAs to a "population" of trial programs, traditionally encoded in memory as tree-structures. Trial programs are estimated using a "fitness function" and the suited solutions picked for re-evaluation and modification such that this sequence is replicated until a "correct" program is generated. GP has represented its power by modifying a simple program for categorizing news stories, executing optical character recognition, medical signal filters, and for target identification, etc. This paper reviews existing literature regarding the GPs and their applications in different scientific fields and aims to provide an easy understanding of various types of GPs for beginners.

Preparation of Nano Flexible Vesicles Encapsulating Adenosine and Composition Optimization by Taguchi Method (아데노신을 포집한 나노 플렉시블 베시클 제조 및 다구찌 방법에 의한 조성의 최적화)

  • Lee, Seo Young;Jin, Byung Suk
    • Applied Chemistry for Engineering
    • /
    • v.30 no.4
    • /
    • pp.487-492
    • /
    • 2019
  • Nano flexible vesicles encapsulating an adenosine, an active ingredient for anti-wrinkle, were prepared for the transdermal delivery. The nano flexible vesicle is usually composed of phospholipid, ethanol, and lysolecithin, which is a type of liquid crystalline one made by dispersing the liquid crystalline phase formed through a hydration process into a water phase. In this study, the Taguchi method, one of the experimental design methods, was applied to investigate the factors affecting the vesicle droplet size. Signal to noise (S/N) ratios for the smaller the better characteristics of vesicle droplet size were calculated using the Taguchi orthogonal array. The composition of ethanol and lysolecithin in the vesicle constituents and the amount of aqueous solution added in the hydration process were main factors that had a great effect on the vesicle droplet size and ANOVA test showed that these factors were significant at 95% confidence level.

Implementation of Ka-band Satellite Broadcasting/LNB with High Dynamic Range (Ka-band 고감도 위성방송용/LNB 최적화 설계)

  • Mok, Gwang-Yun;Lee, Kyung-Bo;Rhee, Young-Chul
    • Proceedings of the Korean Institute of Information and Commucation Sciences Conference
    • /
    • 2016.10a
    • /
    • pp.66-69
    • /
    • 2016
  • In this paper, we suggests a Ka-band LNB considering next-generation UHD satellite TVRO. Since Ka-band has grater attenuation than Ku-band in atmosphere, we designed the low-noise down-converter to improve receiving sensitivity and to extend a dynamic range of receiver. It aims to compensate a quality of ultra high definition transmission signal for rainfall. The low-noise block diagram consists of a three-staged amplifier (LNA), band-pass filter for deleting image (BPF), mixer and IF when considering nonlinear characteristics in the receiver RF front end module. Also, we showed a LNB through optimization processes affecting dynamic range directly in receiver FEM. Asa resuly of experiment, the gain of low-noise down-converter show between 58.5dB and 60.7dB, the noise figure has a high characteristic as 1.38dB. Finally, the phase noise of local oscillator is -63.10dBc at 100MHz offset frequency.

  • PDF