• Journal of the Korea Institute of Information and Communication Engineering
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• v.14 no.10
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• pp.2254-2260
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• 2010

This papers describes modification of the integer transform used in H.264/AVC in order to efficiently apply to lossless compression. The previous reversible integer transform is not efficient for lossless compression due to large dynamic range of the transform coefficients. To reduce the problem, efficient and reversible integer transforms are proposed. The modified transforms are designed based on the lifting scheme for fast transforms. This paper introduces signal flow graphs for the proposed fast transforms and provides corresponding experimental results. The results indicate that the proposed modified reversible integer transform are superior to the previous transform in terms of lossless compression efficiency.

• Molecules and Cells
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• v.25 no.3
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• pp.332-346
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• 2008

S-glutathionylation is a reversible post-translational modification that continues to gain eminence as a redox regulatory mechanism of protein activity and associated cellular functions. Many diverse cellular proteins such as transcription factors, adhesion molecules, enzymes, and cytokines are reported to undergo glutathionylation, although the functional impact has been less well characterized. De-glutathionylation is catalyzed specifically and efficiently by glutaredoxin (GRx, aka thioltransferase), and facile reversibility is critical in determining the physiological relevance of glutathionylation as a means of protein regulation. Thus, studies with cohesive themes addressing both the glutathionylation of proteins and the corresponding impact of GRx are especially useful in advancing understanding. Reactive oxygen species (ROS) and redox regulation are well accepted as playing a role in inflammatory processes, such as leukostasis and the destruction of foreign particles by macrophages. We discuss in this review the current implications of GRx and/or glutathionylation in the inflammatory response and in diseases associated with chronic inflammation, namely diabetes, atherosclerosis, inflammatory lung disease, cancer, and Alzheimer's disease, and in viral infections.

• Speech Sciences
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• v.12 no.4
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• pp.53-70
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• 2005

A Vowel of speech signals are multicomponent signals composed of AM-FM components whose instantaneous frequency and instantaneous amplitude are time-varying. The changes of emotion states cause the variation of the instantaneous frequencies and the instantaneous amplitudes of AM-FM components. Therefore, it is important to estimate exactly the instantaneous frequencies and the instantaneous amplitudes of AM-FM components for the extraction of key information representing emotion states and changes in speech signals. In tills paper, firstly a method decomposing speech signals into AM - FM components is addressed. Secondly, the fundamental frequency of vowel sound is estimated by the simple method based on the spectrogram. The estimate of the fundamental frequency is used for decomposing speech signals into AM-FM components. Thirdly, an estimation method is suggested for separation of the instantaneous frequencies and the instantaneous amplitudes of the decomposed AM - FM components, based on Hilbert transform and the demodulation property of the extended Fourier transform. The estimates of the instantaneous frequencies and the instantaneous amplitudes can be used for modification of the spectral distribution and smooth connection of two words in the speech synthesis systems based on a corpus.

• Proceedings of the KIEE Conference
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• 2007.10a
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• pp.67-69
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• 2007

PMSM (Permanent Magnet Synchronous Motor) current control is a most inner loop of electromechanical driving systems and it plays a foundation role in the hierarchy's control loop of several mechanical machine systems. In this paper, a simple RMRAC control scheme for the PMSM is proposed in the synchronous frame. In the synchronous current model, the input signal is composed of as a calculated voltage by adaptive laws and system disturbances. The gains of feed-forward and feed-back controller are estimated by the proposed e-modification methods respectively, where the disturbances are assumed as filtered current tracking errors. After the estimation of the disturbances from the tracking errors, the corresponding voltage is fed forward to control input to compensate for the disturbances. The proposed method is robust to high frequency disturbances and has a fast dynamic response to time varying reference current trajectory. It also shows a good real-time performance duo to it's simplicity of control structure. Through the simulations considering several cases of external disturbances and experimental results, efficiency of the proposed method is verified

• Applied Chemistry for Engineering
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• v.27 no.2
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• pp.185-189
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• 2016

Among many kinds of bioaffinity sensors, the avidin-biotin system has been widely used in a variety of biological applications due to the specific and high affinity interaction of the system. In this work, gold nanorods with high surface area were explored as electrodes in order to amplify the signal response from the avidin-biotin interaction which can be further utilized for avidin-biotin biosensors. Electrochemical performance of electrodes modified with nanorods and functionalized with avidin in response to interactions with biotin at various concentrations using $[Fe(CN)_6]^{3-/4-}$ couple as the redox probe were investigated using cyclic voltammetry (CV) and electrochemical impedance spectroscopy (EIS). A very low biotin concentration of less than 1 ng/mL could be detected using the electrodes modified with nanorods.

• KSBB Journal
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• v.22 no.4
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• pp.260-264
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• 2007

Peptides are frequently studied as candidates for new drug development. Recently, synthesized peptide library is screened for a certain functionality on a microarray biochip format. In this study, in order to replace the conventional cellulose membrane with glass for a microarray chip substrate for peptide library screening, we modified the glass surface from amines to thiols and covalently immobilized the peptides. Using trypsin-FITC (fluorescein isothiocyanate) conjugate that could specifically bind to a trypsin binding domain consisting of a 7-amino acid peptide, we checked the degree of surface modification. Because of the relatively lower hydrophilicity and reduced surface roughness, the conjugation reaction to the glass required a longer reaction time and a higher temperature. It took approximately 12 hr for the reaction to be completed. From the fluorescence signal intensity, we could differentiate between the target and the control peptides. This difference was confirmed by a separate experiment using QCM. Furthermore, a smaller volume and higher concentration of a spot showed a higher fluorescence intensity. These data would provide the basic conditions for the development of microarray peptide biochips.

• Journal of Life Science
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• v.20 no.11
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• pp.1577-1581
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• 2010

Granulocyte colony stimulating factor (G-CSF) is a hematopoietic cytokine that stimulates bone marrow cells to proliferate and differentiate into granulocytes. G-CSF is approved and used for therapeutic purposes. The endoplasmic reticulum (ER) signal peptide of hG-CSF was replaced with silkworm-specific signal peptides to express and efficiently secrete recombinant hG-CSF by silkworm cells. Plasmids that contain cDNAs for hG-CSF and hG-CSF fused with silkworm- specific signal peptides of prophenoloxidase activating enzyme (PPAE), protein disulfide isomerase (PDI), and bombyxin (BX) were constructed. The G-CSF protein was expressed in insect cell line BM5 and was detected by western blot analysis. The cells transfected with plasmids containing rhG-CSF genes with silkworm-specific signal sequences released mature rhG-CSF protein more efficiently than the cells transfected with pG-CSF, the plasmid containing human G-CSF gene, including its own signal sequence. The production of hG-CSF reached maximal level at four days post-transfection and remained at a high level until 7 days post-transfection. These data demonstrate that the modification of the human G-CSF mimic to insect proteins synthesized in ER greatly improves the production of the protein.

• Journal of Life Science
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• v.23 no.4
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• pp.586-594
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• 2013

Post-translational O-GlcNAc modification (O-GlcNAcylation) of serine or threonine is a new protein modulation mechanism. In contrast to the classical glycosylation, O-GlcNAcylation occurs in a one-step transfer of O-GlcNAc on both nuclear and cytoplasmic proteins. In contrast to the general consensus that O-GlcNAc is a final modification, a recent paper (J Proteome Res. 2011 10:2725-2733) showed the presence of O-GlcNAc-P on a synaptic assembly protein AP180. This finding raises a fundamental question about its prevalence. To address this question, we used proteomics to identify those proteins that were phospho-signal enriched by GlcNAc kinase (NAGK). Comparison of pDsRed2-$NAGK_{WT}$-transfected HEK293T cell extract with pDsRed2-$NAGK_{D107A}$-transfected control culture revealed 15 phospho-signal increased spots. Excluding those spots that had no detectable amount of protein expression yielded 7 spots, which were selected for ID determination. Among these, two duplicate spots (two $HSP90{\beta}$ and two ENO1 spots) were shown to be O-GlcNAcylated, two (dUTP nucleotidohydrolase mitochondrial isoform 2, glutathione S-transferase P) were not known to be involved in O-GlcNAcylation, and one (heat shock protein gp96 precursor or grp94) was a glycoprotein. The increase in the phospho-levels of O-GlcNAc by NAGK strongly indicates that these proteins are phosphorylated on O-GlcNAc. Our present data support the idea that O-GlcNAc is not a terminal modification.

• Journal of Microbiology and Biotechnology
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• v.28 no.3
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• pp.439-447
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• 2018

The aromatic compound p-hydroxybenzoate (PHBA) is an important material with multiple applications, including as a building block of liquid crystal polymers in chemical industries. The cytochrome P450 (CYP) enzymes are beneficial monooxygenases for the synthesis of chemicals, and CYP53A15 from fungus Cochliobolus lunatus is capable of executing the hydroxylation from benzoate to PHBA. Here, we constructed a system for the bioconversion of benzoate to PHBA in Escherichia coli cells coexpressing CYP53A15 and human NADPH-P450 oxidoreductase (CPR) genes as a redox partner. For suitable coexpression of CYP53A15 and CPR, we originally constructed five plasmids in which we replaced the N-terminal transmembrane region of CYP53A15 with a portion of the N-terminus of various mammalian P450s. PHBA productivity was the greatest when CYP53A15 expression was induced at $20^{\circ}C$ in $2{\times}YT$ medium in host E. coli strain ${\Delta}gcvR$ transformed with an N-terminal transmembrane region of rabbit CYP2C3. By optimizing each reaction condition (reaction temperature, substrate concentration, reaction time, and E. coli cell concentration), we achieved 90% whole-cell conversion of benzoate. Our data demonstrate that the described novel E. coli bioconversion system is a more efficient tool for PHBA production from benzoate than the previously described yeast system.

• Journal of the Institute of Electronics Engineers of Korea SP
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• v.47 no.1
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• pp.175-182
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• 2010

In this paper, we propose a robust speech reinforcement technique to enhance the intelligibility of the degraded speech signal under the ambient noise environments based on soft decision scheme incorporating a speech absence probability (SAP) with speech reinforcement gains. Since the ambient noise significantly decreases the intelligibility of the speech signal, the speech reinforcement approach to amplify the estimated clean speech signal from the background noise environments for improving the intelligibility and clarity of the corrupted speech signal was proposed. In order to estimate the robust reinforcement gain rather than the conventional speech reinforcement method between speech active periods and nonspeech periods or transient intervals, we propose the speech reinforcement algorithm based on soft decision applying the SAP to the estimation of speech reinforcement gains. The performances of the proposed algorithm are evaluated by the Comparison Category Rating (CCR) of the measurement for subjective determination of transmission quality in ITU-T P.800 under various ambient noise environments and show better performances compared with the conventional method.