• Nuclear Engineering and Technology
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• v.20 no.1
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• pp.47-53
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• 1988

The objective of this study is to evaluate the reliability of the High Head Safety Injection System (HHIS) of KNU 5, 6, 7 and 8 following the removal of safety injection signal (s-signal) from the mini-flow bypass line valves of charging/safety injection pumps. The unavailability of HHSIS and the rupture probability of a charging/safety injection pump have been computed for two different cases; with s-signal on and removed. The results show that when the s-signal is removed from the mini-flow bypass line valves, the unavailability of HHSIS slightly increases while the rupture probability of a charging/safety injection pump is significantly reduced. Hence, based upon the results of this study we conclude that it is more reasonable to remove the s-signal from the mini-flow bypass line valves of KNU 5, 6, 7 and 8 in the normal plant operation. And to improve the availability of HHSIS, the modification of operational procedures and the emphasis on operator training are recommended.

• Bulletin of the Korean Chemical Society
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• v.31 no.6
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• pp.1527-1534
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• 2010

The conventional peptide modification process of guanidination, in which the amino groups of lysine residues are converted to guanidino groups using O-methylisourea to create more basic homoarginine residues, is often used to improve the signal intensity of lysine-containing peptides in matrix-assisted laser desorption/ionization mass spectrometry (MALDI-MS). Here, we used three different protease enzymes (trypsin, Lys-C, and Glu-C) to evaluate the effects of guanidination on the MS signals of two enzymatically digested proteins. Horse heart myoglobin and bovine serum albumin were guanidinated either before or after digestion with trypsin, Lys-C, or Glu-C. The resulting peptides were subjected to MALDI-MS, and signal intensities and sequence coverage were systematically evaluated for each digest. Guanidination prior to Glu-C digestion improved sequence coverage for both proteins. For myoglobin, guanidination before enzymatic digestion with trypsin or Lys-C also enhanced sequence coverage, but guanidination after enzymatic digestion enhanced sequence coverage only with Lys-C. For albumin, guanidination either before or after Glu-C digestion increased sequence coverage, whereas pre- or post-digestion guanidination decreased sequence coverage with trypsin and Lys-C. The amino acid composition of a protein appears to be the major factor determining whether guanidination will enhance its MALDI-MS sequence coverage.

• Nuclear Engineering and Technology
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• v.49 no.5
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• pp.1100-1108
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• 2017

A thermal-hydraulic system code is an essential tool for the design and safety analysis of a nuclear power plant, and its accuracy quantification is very important for the code assessment and applications. The fast Fourier transform-based method (FFTBM) by signal mirroring (FFTBM-SM) has been used to quantify the accuracy of a system code by using a comparison of the experimental data and the calculated results. The method is an improved version of the FFTBM, and it is known that the FFTBM-SM judges the code accuracy in a more consistent and unbiased way. However, in some applications, unrealistic results have been obtained. In this study, it was found that accuracy quantification by FFTBM-SM is dependent on the frequency spectrum of the fast Fourier transform of experimental and error signals. The primary objective of this study is to reduce the frequency dependency of FFTBM-SM evaluation. For this, it was proposed to reduce the cut off frequency, which was introduced to cut off spurious contributions, in FFTBM-SM. A method to determine an appropriate cut off frequency was also proposed. The FFTBM-SM with the modified cut off frequency showed a significant improvement of the accuracy quantification.

• The Journal of the Acoustical Society of Korea
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• v.37 no.6
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• pp.457-467
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• 2018

This paper presents performance comparison of several high-resolution frequency estimation algorithms for pure real tone signal. Algorithms are DFT (Discrete Fourier Transform - for reference purpose), Jacobsen, Candan, reassignment and Cedron. Each algorithm is evaluated under various experimental conditions, e.g., different SNR (Signal to Noise Ratio), window function and window length and performance is compared in the perspective of bias, MSE (Mean Square-Error) and variance. Experimental results indicate that Cedron algorithms works well in the most cases. For actual usage in the engineering problem, each algorithm needs additional analysis and modification.

• The Journal of the Acoustical Society of Korea
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• v.31 no.7
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• pp.427-434
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• 2012

The overlap-add technique based on waveform similarity (WSOLA) method is known to be an efficient high-quality algorithm for time scaling of speech signal. The computational load of WSOLA is concentrated on the repeated normalized cross-correlation (NCC) calculation to evaluate the similarity between two signal waveforms. To reduce the computational complexity of WSOLA, this paper proposes a fast NCC computation method, in which NCC is obtained through pre-calculated sum tables to eliminate redundancy of repeated NCC calculations in the adjacent regions. While the denominator part of NCC has much redundancy irrespective of the time-scale factor, the numerator part of NCC has less redundancy and the amount of redundancy is dependent on both the time-scale factor and optimal shift value, thereby requiring more sophisticated algorithm for fast computation. The simulation results show that the proposed method reduces about 40%, 47% and 52% of the WSOLA execution time for the time-scale compression, 2 and 3 times time-scale expansions, respectively, while maintaining exactly the same speech quality of the conventional WSOLA.

• Animal cells and systems
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• v.11 no.1
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• pp.39-50
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• 2007

Tau plays a role in numerous neuronal processes, such as vesicle transport, microtubule-plasma membrane interaction and intracellular localization of proteins. SUMO (Small Ubiquitin-like Modifier) modification (SUMOylation) appears to regulate diverse cellular processes including nuclear transport, signal transduction, apoptosis, autophagy, cell cycle control, ubiquitin-dependent degradation, as well as gene transcription. We noticed that putative SUMOylation site is localized at $^{340}K$ of $Tau(^{339}VKSE^{342})$ with the consensus sequence information (${\Phi}KxE$ ; where ${\Phi}$ represents L, I, V or F and x is any amino acid). In this report, we demonstrated that $^{340}K$ of Tau is the SUMOylation site and that a point mutant of Tau S214E (an analog of the phospho $^{214}S$ Tau) promotes its SUMOylation at $^{340}K$ and its nuclear or nuclear vicinity localization, by co-immunoprecipitation and confocal microscopy analysis. Further, we demonstrate that the Tau S214E (neither Tau S214A nor Tau K340R) mutant increases its protein stability. However, the SUMOylation at $^{340}K$ of Tau did not influence cell survival, as determined by FACS analysis. Therefore, our results suggested that the phosphorylation of Tau on $^{214}S$ residue promotes its SUMOylation on $^{340}K$ residue and nuclear vicinity localization, and increases its stability, without influencing cell survival.

• Journal of Life Science
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• v.28 no.3
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• pp.376-387
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• 2018

Among a variety of environmental stresses heat, cold, chilling, high salt, drought, and so on exposed to plants, drought stress has been reported as a crucial factor to adversely affect the growth and productivity of plants. Therefore, to understand the mechanism for the drought stress signal transduction pathway in plants is more helpful to develop useful crops that display the enhanced tolerance against drought stress, and to expand crop growing areas. The signal transduction pathway for the drought stress in plants is largely categorized into two types; ABA-dependent pathway and ABA-independent pathway. It has been reported that two transcription factors, AREB/ABF and DREB2, play predominant roles in ABA-dependent and ABA-independent pathways, respectively. In addition to transcriptional regulation mediated by AREB/ABF and DREB2 transcription factors, post-translational modification (such as phosphorylation and ubiquitination) and epigenetic control are importantly involved in the signal transduction for drought stress. In this paper, we review current understanding of signal transduction pathway on drought stress in plants, especially focusing on the biological roles of a variety of signaling components related to drought stress response. Further understanding the mechanism of drought resistance in plants through this review will be useful to establish theoretical basis for developing drought tolerant crops in the future.

• Mass Spectrometry Letters
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• v.7 no.2
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• pp.50-54
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• 2016

Multi-collector inductively coupled plasma mass spectrometry (MC-ICP-MS) is currently used in our laboratory for isotopic and quantitative analyses of nuclear materials at ultra-trace levels in environmental swipe samples, which is a very useful for monitoring undeclared nuclear activities. In this study, to improve measurement precisions of uranium isotopes at ultratrace levels, we adopted a desolvating nebulizer system (Aridus-II, CETAC., USA), which can improve signal sensitivity and reduce formation of uranium hydride. A peristaltic pump was combined with Aridus-II in the sample introduction system of MC-ICP-MS to reduce long-term signal fluctuations by maintaining a constant flow rate of the sample solution. The signal sensitivity in the presence of Aridus-II was improved more than 10-fold and the formation ratio of UH/U decreased by 16- to 17- fold compared to a normal spray chamber. Long-term signal fluctuations were significantly reduced by using the peristaltic pump. Detailed optimizations and evaluations with uranium standards are also discussed in this paper.

• Biotechnology and Bioprocess Engineering:BBE
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• v.7 no.5
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• pp.237-251
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• 2002

The recent progress on metabolic systems engineering was reviewed based on our recent research results in terms of (1) metabolic signal flow diagram approach, (2) metabolic flux analysis (MFA) in particular with intracellular isotopomer distribution using NMR and/or GC-MS, (3) synthesis and optimization of metabolic flux distribution (MFD), (4) modification of MFD by gene manipulation and by controlling culture environment, (5) metabolic control analysis (MCA), (6) design of metabolic regulation structure, and (7) identification of unknown pathways with isotope tracing by NMR. The main characteristics of metabolic engineering is to treat metabolism as a network or entirety instead of individual reactions. The applications were made for poly-3-hydroxybutyrate (PHB) production using Ralstonia eutropha and recombinant Escherichia coli, lactate production by recombinant Saccharomyces cerevisiae, pyruvate production by vitamin auxotrophic yeast Toluropsis glabrata, lysine production using Corynebacterium glutamicum, and energetic analysis of photosynthesic microorganisms such as Cyanobateria. The characteristics of each approach were reviewed with their applications. The approach based on isotope labeling experiments gives reliable and quantitative results for metabolic flux analysis. It should be recognized that the next stage should be toward the investigation of metabolic flux analysis with gene and protein expressions to uncover the metabolic regulation in relation to genetic modification and/ or the change in the culture condition.

• Journal of the Korean Institute of Telematics and Electronics
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• v.17 no.1
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• pp.23-30
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• 1980

The reconstruction problem in a low dose case requires some compromise of resolution and noise artifacts, and also some modification of filter kernels depending on the signal-to-noise ratio of projection data. In this paper, ail algorithm for the reconstruction of an image function from noisy projection data is suggested, based on minimum-mean-square error criterion. Modification of the falter kernel is made from information (statistics) obtained from the projection data. The simulation study Proves that this algorithm, based on the Wiener falter approach, provides substantially improved image with reduction of noise as well as improvement of the resolution. An approximate method was also studied which leads to the possible use of a recursive filter in the convolution process of image reconstruction.