• Journal of Animal Science and Technology
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• v.45 no.1
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• pp.13-22
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• 2003

Growth Hormone (GH) gene is a member of gene family through the evolutionary process from a small common ancestral gene by a series of gene duplications. The role of the GH in growth and performance controls has been extensively studied in human, mice and livestock. Many researchers have considered GH as a strong candidate gene for evaluation of genetic polymorphisms that could be associated with economic traits in cattle. We report here a novel missense mutation within the exon 5 of the bovine Growth Hormone (bGH) gene. We could amplified 522 bp fragments from eight unrelated Hanwoo cattle by PCR, then, subsequently cloned and sequenced. An Msp I RFLP corresponding to a C to T transition was observed at position 2258 nt. From this result, we could predict a missense mutation (Arg to Trp) at codon 166 in a highly conserved region among many mammals. Codominant Mendelian segregation of the two alleles, Msp I (+) and Msp I (－), was observed in two full-sib F2 families (n = 32, African taurine Bos taurus ${\times}$ African zebu Bos indicus) and eight half-sib Hanwoo families. For the availability of genetic marker, we have performed PCR-RFLP with a large number of individual animals from 15 different cattle breeds (European and Asian taurines, and African indicines). Consideration of breed frequencies of Msp I (－) allele in relation to breed type and their geographic origins, shows higher frequencies in humped breeds or Asian cattle breeds than in humpless or European breeds. This result indicates that the missense mutation can be contributed the functional significance such as the signal transduction through the receptor binding, also may be used as a marker for selection of the economic traits in Hanwoo.

• Food Science and Biotechnology
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• v.16 no.4
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• pp.515-519
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• 2007

Rapid immunochromatographic assay (ICA) kits were developed using flagella-specific monoclonal antibodies (MAbs) and rabbit polyclonal antibodies for screening Listeria spp. in food. The establishment of different formats, MAb 2B1 as capture antibody and MAb 7A3 or rabbit polyclonal antibodies as detector antibody, was compared. The 2 formats of the ICA kit were shown to have specific reactions with Listeria and no cross-reactivity with any of the non-Listeria including Escherichia coli O157:H7 and Salmonella enteritidis. The detection limits of the ICA kit using the combination of gold-labeled MAb 7A3 and MAb 2B1 showed $1{\times}10^5$ and $1{\times}10^6\;CFU/0.1\;mL$ at 22 and $30^{\circ}C$, respectively. The other format of the ICA kit using the combination of gold-labeled rabbit polyclonal antibodies and MAb 2B1 showed $1{\times}10^6\;CFU/0.1\;mL$ at $22^{\circ}C$ but weak signal at 30 culture. The format utilizing MAb was more sensitive than the one using polyclonal antibodies for capture antibody. Samples contaminated with L. monocytogenes 4b culture (9-10, 5-6, and 1-2 CFU/mL) on pork and pasteurized milk were confirmed as positive results. Current data suggests that this ICA kit is a rapid, simple and effective tool to screen for Listeria spp. in food.

• Geophysics and Geophysical Exploration
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• v.12 no.1
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• pp.69-76
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• 2009

We report an implementation of the Ground Penetrating Radar (GPR) survey at a site that corresponds to a ruined castle. The objective of the survey was to characterise buried archaeological structures such as walls and tiles in Van Khan Tooril's Ruin, Mongolia, by 2D and 3D GPR techniques. GPR datasets were acquired in an area 10mby 9 m, with 10 cm line spacing. Two datasets were collected, using GPR with 500MHz and 800MHz frequency antennas. In this paper, we report the use of instantaneous parameters to detect archaeological targets such as tile, brick, and masonry by polarimetric GPR. Radar polarimetry is an advanced technology for extraction of target scattering characteristics. It gives us much more information about the size, shape, orientation, and surface condition of radar targets. We focused our interpretation on the strongest reflections. The image is enhanced by the use of instantaneous parameters. Judging by the shape and the width of the reflections, it is clear that moderate to high intensity response in instantaneous amplitude corresponds to brick and tiles. The instantaneous phase map gave information about the location of the targets, which appeared as discontinuities in the signal. In order to increase our ability to interpret these archaeological targets, we compared the GPR datasets acquired in two orthogonal survey directions. A good correlation is observed for the alignments of reflections when we compare the two datasets. However, more reflections appear in the north-south survey direction than in the west-east direction. This is due to the electric field orientation, which is in the horizontal plane for north-south survey directions and the horizontally polarised component of the backscattered high energy is recorded.

• Korean Journal of Agricultural and Forest Meteorology
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• v.14 no.4
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• pp.143-154
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• 2012

As demand of water resources and attentions to changes in climate (e.g., due to ENSO) increase, long/short term prediction of precipitation is getting necessary in water planning. This research evaluated the ability of MM5 to predict precipitation in the Tampa Bay region over 23 year period from 1986 to 2008. Additionally MM5 results were statistically bias-corrected using observation data at 33 stations over the study area using CDF-mapping approach and evaluated comparing to raw results for each ENSO phase (i.e., El Ni$\tilde{n}$o and La Ni$\tilde{n}$a). The bias-corrected model results accurately reproduced the monthly mean point precipitation values. Areal average daily/monthly precipitation predictions estimated using block-kriging algorithm showed fairly high accuracy with mean error of daily precipitation, 0.8 mm and mean error of monthly precipitation, 7.1 mm. The results evaluated according to ENSO phase showed that the accuracy in model output varies with the seasons and ENSO phases. Reasons for low predictions skills and alternatives for simulation improvement are discussed. A comprehensive evaluation including sensitivity to physics schemes, boundary conditions reanalysis products and updating land use maps is suggested to enhance model performance. We believe that the outcome of this research guides to a better implementation of regional climate modeling tools in water management at regional/seasonal scale.

• Journal of Digital Convergence
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• v.14 no.9
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• pp.331-339
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• 2016

Chronic diseases are increasing nowadays as daily habits changed due to economic growth. Among chronic diseases, heart cerebrovascular disease is one of the major causes of death in South Korea that accounts for approximately 20% of mortality. Tests to measure anomaly of the heart is ECG tests, which measures and analyzes the electrical heart activity. Any mistakes in lead attachment location critically affects ECG testings, and statistical facts showed that only 2.8% of the nurses properly located leads to patients. As a solution, this paper proposes a system based on a projection-based augmented reality technology to generate X-ray images to the patient's chest to point out exact attachment locations of ECG leads. Evaluation comparison results showed a 2.6 cm difference between the conventional system and the proposed system. ECG test results also showed significant signal differences between the systems in leads V1, V2, and V3. The ECG measured accurately by the proposed system would help greatly in patient management and clinical decisions of clinicians.

• Journal of the Institute of Electronics Engineers of Korea SP
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• v.46 no.4
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• pp.124-131
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• 2009

A reliable detection of regions in radiography is one of the most important task before the evaluation of defects on welded joints. The extracted features is to be classified into distinctive clusters for each segmented region. But conventional segmentation techniques give unsatisfactory results for this task due to the spatial superposition of intensity and low signal-to-ratio(SNR) in radiographic images. The usage of global or local processes not only provide the necessary noise resistance but also fail in classification of regions. In this paper, we presents an appropriate approach for segmentation of region-based indications in industrial Computed Radiography(CR) images. The geometric differences between welded and non-welded area which is generated on radiography as the representative regions(background, thickness, middle and welded region in steel tube image) have constructed the hierarchical structure. Although this structure is contaminated by noise, the scheme between regions can be selected by the help of local clustering based on distinctive geometric property of each region. Because of the geometric nature of the considered region and so that the region is selected layer by layer, and that the real class represents the boundary between regions, the vertical and horizontal clustering process in each layer must be judicious. In order to show the effectiveness of this approach, a comparative experiment of various segmentation method is performed on industrial steel tube CR images.

• Journal of the Institute of Electronics and Information Engineers
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• v.50 no.11
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• pp.12-20
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• 2013

Even though extensive research results have been applied to wireless cellular systems to improve their capacity and coverage, severe performance degradation experienced in cell boundary areas still remains as a major limiting factor to prohibit further improvement of user equipment (UE) throughput. In the Long Term Evolution-Advanced (LTE-A) standard of the Third Generation Partnership Project (3GPP), Some advanced techniques have been introduced to overcome this "cell-edge problem", including coordinated multipoint transmission and reception (CoMP) and inter-cell interference coordination (ICIC). In this paper, we propose yet another strategy to improve the performance of low-tier UEs by using the concept of multiple beam direction patterns (BDPs). Such multiple BDPs can be implemented using multi-layer antenna arrays stacked vertically at base station (BS) sites to transmit signals in different main beam directions. In comparison to conventional three-sector antennas with a fixed beam pattern, the proposed methods makes signal transmission in a rotational fashion to significantly enhance the reception quality of UEs located near sector (or cell) edge areas, preventing the situation where certain UEs are marginally covered by the BS for the whole transmission time. Performance evaluation results show that the proposed scheme outperforms the conventional three-sector transmission by 171% in low 5% UEs in terms of the UE throughput.

• Composites Research
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• v.28 no.4
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• pp.232-238
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• 2015

As a new method to predict the degree of dispersion in carbon nanocomposites, the electrical resistance (ER) method has been evaluated. After CNT epoxy resin was dropped on CF tow, the change in electrical resistance of carbon fiber tow was measured to evaluate dispersion condition in CNT epoxy resin. Good dispersion of CNTs in carbon nanocomposite exhibited low change in ER due to wetted resin penetrated on CF tow. However, because CNT network was formed among CFs, non-uniform dispersion occurred due to nanoparticle filtering effect by CF tow. The change in ER for poor dispersion exhibited large ER signal change. The change in ER was used for the dispersion evaluation of CNT epoxy resin. Correlation between interlaminar shear strength (ILSS) and dispersion condition by ER method was established. Good CNT dispersion in nanocomposites led to good interfacial properties of fiberreinforced nanocomposites.

• Journal of the Korean Chemical Society
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• v.48 no.6
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• pp.553-560
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• 2004

In this experiment, a three dimensional structure analysis was carried out to examine the surface defects of semiconductor made artificially on known scale. It was investigated the three dimensional imaging according to the sample depth and the thermal diffusivity as well as the carrier transport properties. The thermal diffusivity measurement of the intrinsic GaAs semiconductor was also analyzed by the difference of frequency-dependence photoacoustic signals from the sample surface of different conditions. Thermal properties such as thermal diffusion length or thermal diffusivity of the Si wafer with and without defects on the surface were obtained by interpreting the frequency dependence of the PA signals. As a result, the photoacoustic signal is found to have the dependency on the shape and depth of the defects so that their structure of the defects can be analyzed. This method demonstrates the possibility of the application to the detection of the defects, cracks, and shortage of circuits on surface or sub-surface of the semiconductors and ceramic materials as a nondestructive testing(NDT) and a nondestructive evaluation(NDE) technique.

• Journal of the Korean Chemical Society
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• v.39 no.12
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• pp.902-909
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• 1995

Detection limit as well as calibration curves on organophosphorus pesticide(dimethoate, diazinon, parathion-methyl, fenitrothion, malathion) and BHCs were measured for evaluation of utility on qualitative or quantitative analysis of pesticides with ion Trap mass spectrometer and quadrupole mass spectrometer. As ionization source, EI and CI were adopted for qualitative analysis of pesticides by comparison of each fragmentation pattern. At the same time, the utility as trace analysis techniques through scan or selected ion monitoring(SIM) mode was evaluated. With ion trap for all pesticides, detection limit(DL, 1 ${\mu}L$ injection) on scan mode was ranged 0.008∼0.225 ng at signal to noise ratio 3. With quadrupole DL on scan mode was ranged 0.23∼3.1 ng over 0.032∼0.68 ng on SIM mode. The calibration curve with ion trap generated good linearity over 0.99 as correlation coefficient. As clean up procedure, Bio Beads S-X3 was used for the separation of oils from five organophosphorous pesticides in flour extractant showing more than 80% as recovery at most cases. In case of BHCs in jinseng with Florisil column, the recovery of pesticides has been 60% to 90%.