• Proceedings of the Korean Vacuum Society Conference
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• 2013.08a
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• pp.88-89
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• 2013

A variety of influenza A viruses from animal hosts are continuously prevalent throughout the world which cause human epidemics resulting millions of human infections and enormous industrial and economic damages. Thus, early diagnosis of such pathogen is of paramount importance for biomedical examination and public healthcare screening. To approach this issue, here we propose a fully integrated Rotary genetic analysis system, called Rotary Genetic Analyzer, for on-site detection of influenza A viruses with high speed. The Rotary Genetic Analyzer is made up of four parts including a disposable microchip, a servo motor for precise and high rate spinning of the chip, thermal blocks for temperature control, and a miniaturized optical fluorescence detector as shown Fig. 1. A thermal block made from duralumin is integrated with a film heater at the bottom and a resistance temperature detector (RTD) in the middle. For the efficient performance of RT-PCR, three thermal blocks are placed on the Rotary stage and the temperature of each block is corresponded to the thermal cycling, namely $95^{\circ}C$ (denature), $58^{\circ}C$ (annealing), and $72^{\circ}C$ (extension). Rotary RT-PCR was performed to amplify the target gene which was monitored by an optical fluorescent detector above the extension block. A disposable microdevice (10 cm diameter) consists of a solid-phase extraction based sample pretreatment unit, bead chamber, and 4 ${\mu}L$ of the PCR chamber as shown Fig. 2. The microchip is fabricated using a patterned polycarbonate (PC) sheet with 1 mm thickness and a PC film with 130 ${\mu}m$ thickness, which layers are thermally bonded at $138^{\circ}C$ using acetone vapour. Silicatreated microglass beads with 150~212 ${\mu}L$ diameter are introduced into the sample pretreatment chambers and held in place by weir structure for construction of solid-phase extraction system. Fig. 3 shows strobed images of sequential loading of three samples. Three samples were loaded into the reservoir simultaneously (Fig. 3A), then the influenza A H3N2 viral RNA sample was loaded at 5000 RPM for 10 sec (Fig. 3B). Washing buffer was followed at 5000 RPM for 5 min (Fig. 3C), and angular frequency was decreased to 100 RPM for siphon priming of PCR cocktail to the channel as shown in Figure 3D. Finally the PCR cocktail was loaded to the bead chamber at 2000 RPM for 10 sec, and then RPM was increased up to 5000 RPM for 1 min to obtain the as much as PCR cocktail containing the RNA template (Fig. 3E). In this system, the wastes from RNA samples and washing buffer were transported to the waste chamber, which is fully filled to the chamber with precise optimization. Then, the PCR cocktail was able to transport to the PCR chamber. Fig. 3F shows the final image of the sample pretreatment. PCR cocktail containing RNA template is successfully isolated from waste. To detect the influenza A H3N2 virus, the purified RNA with PCR cocktail in the PCR chamber was amplified by using performed the RNA capture on the proposed microdevice. The fluorescence images were described in Figure 4A at the 0, 40 cycles. The fluorescence signal (40 cycle) was drastically increased confirming the influenza A H3N2 virus. The real-time profiles were successfully obtained using the optical fluorescence detector as shown in Figure 4B. The Rotary PCR and off-chip PCR were compared with same amount of influenza A H3N2 virus. The Ct value of Rotary PCR was smaller than the off-chip PCR without contamination. The whole process of the sample pretreatment and RT-PCR could be accomplished in 30 min on the fully integrated Rotary Genetic Analyzer system. We have demonstrated a fully integrated and portable Rotary Genetic Analyzer for detection of the gene expression of influenza A virus, which has 'Sample-in-answer-out' capability including sample pretreatment, rotary amplification, and optical detection. Target gene amplification was real-time monitored using the integrated Rotary Genetic Analyzer system.

• Progress in Medical Physics
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• v.21 no.3
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• pp.261-273
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• 2010

For the use of Indirect-conversion CMOS (complementary metal-oxide-semiconductor) detectors for digital x-ray radiography and their better designs, we have theoretically evaluated the spatial-frequency-dependent detective quantum efficiency (DQE) using the cascaded linear-systems transfer theory. In order to validate the developed model, the DQE was experimentally determined by the measured modulation-transfer function (MTF) and noise-power spectrum, and the estimated incident x-ray fluence under the mammography beam quality of W/Al. From the comparison between the theoretical and experimental DQEs, the overall tendencies were well agreed. Based on the developed model, we have investigated the DQEs values with respect to various design parameters of the CMOS x-ray detector such as phosphor quantum efficiency, Swank noise, photodiode quantum efficiency and the MTF of various scintillator screens. This theoretical approach is very useful tool for the understanding of the developed imaging systems as well as helpful for the better design or optimization for new development.

• Journal of Korean Ophthalmic Optics Society
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• v.6 no.2
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• pp.71-75
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• 2001

In this paper, a three-dimensional measuring system of thermoluminescence(TL) spectra based on temperature, wavelength and luminescence intensity was introduced. The system was composed of a spectrometer, temperature control unit for thermal stimulation, photon detector and personal computer for control the entire system. Temperature control was achieved by using feedback to ensure a linear-rise in the sample temperature. Digital multimeter(KEITHLEY 195A) measures the electromotive force of Copper-Constantan thermocouple and then transmits the data to the computer through GPIB card. The computer converts this signal to temperature using electromotive force-temperature table in program, and then control the power supply through the D/A converter. The spectrometer(SPEX 1681) is controlled by CD-2A, which is controlled by the computer through RS-232 communication port. For measuring the luminescence intensity during the heating run, the electrometer(KEITHLEY 617) measures the anode current of photomultiplier tube(HAMAMATSU R928) and transmits the data to computer through the A/D converter. And, we measured and analyzed thermoluminescence of $CaSO_4$ : Dy, P using the system. The measuring range of thermoluminescence spectra was 300K-575K and 300~800 nm, $CaSO_4$ : Dy. P was fabricated by the Yamashita's method in Korea Atomic Energy Research Institute(KAERI) for radiation dosimeter. Thermoluminesce spectra of the $CaSO_4$ : Dy, P consist of two main peak at temperature of $205^{\circ}C$, wavelength 476 nm and 572 nm and with minor ones at 658 nm and 749 nm.

• Progress in Superconductivity
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• v.6 no.1
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• pp.56-63
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• 2004

We consider design factors for a SQUID sensor array to construct a 52-channel magnetocardiogram (MCG) system that can be used to measure tangential components of the cardiac magnetic fields. Nowadays, full-size multichannel MCG systems, which cover the whole signal area of a heart, are developed to improve the clinical analysis with high accuracy and to provide patients with comfort in the course of measurement. To design the full-size MCG system, we have to make a compromise between cost and performance. The cost is involved with the number of sensors, the number of the electronics, the size of a cooling dewar, the consumption of refrigerants for maintenance, and etc. The performance is the capability of covering the whole heart volume at once and of localizing current sources with a small error. In this study, we design the cost-effective arrangement of sensors for MCG by considering an adequate sensor interval and the confidence region of a tolerable localization error, which covers the heart. In order to fit the detector array on the cylindrical dewar economically, we removed the detectors that were located at the corners of the array square. Through simulations using the confidence region method, we verified that our design of the detector array was good enough to obtain whole information from the heart at a time. A result of the simulation also suggested that tangential-component MCG measurement could localize deeper current dipoles than normal-component MCG measurement with the same confidence volume; therefore, we conclude that measurement of the tangential component is more suitable to an MCG system than measurement of the normal component.

• The Journal of the Institute of Internet, Broadcasting and Communication
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• v.15 no.2
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• pp.263-267
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• 2015

current mine detector can't division the section if it is conducted and it needs too much labor force and time. in addition to, if the user don't move the head of sensor in regular speed or move it too fast, it is hard to detect a mine exactly. according to this, to improve the problem using one direction ultrasonic wave sensing signal, that is made up of human body antenna part, main micro processor unit part, smart glasses part, body equipped LCD monitor part, wireless data transmit part, belt type power supply part, black box type camera, Security Communication headset. the user can equip this at head, body, arm, waist and leg in removable type. so it is able to detect the powder in a 360-degree on(under) the ground whether it is metal or nonmetal and it can express the 2D or 3D film about distance, form and material of the mine. so the battle combats can avoid the mine and move fast. also, through the portable battery and twin self power supply system of the power supply part, combat troops can fight without extra recharge and we can monitoring the battle situation of distant place at the command center server on real-time. and then, it makes able to sharing the information of battle among battle combats one on one. as a result, the purpose of this study is researching a smart wearable mine detector which can establish a smart battle system as if the commander is in the site of the battle.

• Journal of the Korean Society of Radiology
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• v.14 no.5
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• pp.517-522
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• 2020

Electron beam have many factors that affect dose distribution, so even if identical settings are used, they should be identified and used for radiation treatment, and the effects on the structures in the body are sensitive, making it difficult to investigate uniform dose distribution on tumors. In this study, a dosimeter was produced using PbI₂ which is a photoelectric material, and electrical characteristics were analyzed for 6, 9, and 12 MeV electronics in linear accelerators. The reproducibility test results showed that RSD were 1.1215%, 1.0160%, and 0.05137% respectively at 6, 9, and 12 MeV energies, indicating that the output signals were stable. The linearity evaluation results showed that the R² values of the reliability indicator for straight line trend lines were 0.9999, 0.9999, and 0.9994, respectively, at 6, 9, and 12 MeV, to confirm that the output signal was proportional to PbI₂ as dose increased. The PbI₂ dosimeter in this study is judged to be highly applicable to electromagnet measurement and is thought to be able to be used as a basic study of electron detector through photoelectric material.

• The Journal of Korean Institute of Communications and Information Sciences
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• v.41 no.5
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• pp.574-580
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• 2016

In this paper, we will introduce the system that can control multiple vehicles on the road through Single Board Computers and V2I (Vehicle-To-Infrastructure). Also, we will propose the group evasive maneuver decision algorithm, which plays a critical role in deciding whether the vehicles in the system have to conduct evasive maneuvers to avoid obstacles on the road. In order to test this system, we have utilized Wi-Fi and TCP/IP for establishing the communication between multiple vehicles and the relay server, and observed their driving states on the road with obstacles. During the experiments, we have discovered that our original decision algorithm possesses high failure rate when there is frequency interference in ISM (Industrial Scientific Medical) band. In order to reduce this failure rate, we have implemented the data transition detector. This paper will focus on how the use of data transition detector can affect the reliability of the system under the frequency interference of ISM band. If this technology is improved and applied in the field, we will effectively deal with such dangerous situations as multiple collision accidents through vehicle-to-vehicle communication or vehicle-to-infrastructure communication. Furthermore, this can be applied to the autonomous driving technologies. This can be used as the reference data for the development of the similar system.

• Journal of the Korean Society of Radiology
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• v.3 no.1
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• pp.11-15
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• 2009

In this study, the basic research verifying possibility of applications as radiology image sensor in Digital Radiography was performed, the radiology image sensor was fabricated using a multi-layer technique to decrease dark current. High efficiency materials in substitution for Amorphous Selenium(a-Se) have been studied as a direct method of imaging detector in Digital Radiography to decrease dark current by using PN junction or Hetero junction already used as solar cell, semiconductor. Particle-In -Binder method is used to fabricate radiology image sensor because it has a lot of advantages such as fabrication convenient, high yield, suitability for large area sensor. But high leakage current is one of main problem in Particle-In -Binder method. To make up for the weak points, multi-layer technique is used, and it is considered that high efficient digital radiation sensor can be fabricated with easy and convenient process. In this study, electrical properties such as leakage current, sensitivity, signal linearity is measured to evaluate multi-layer radiation sensor material.

• Journal of Sensor Science and Technology
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• v.11 no.2
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• pp.67-76
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• 2002

We have presented a 2-dimensional fluxgate sensor with ferrite core, excitation, and pick-up coil. This fluxgate sensor system consists of a sensing element, driving circuits for excitation coil and signal processing for detecting second harmonic frequency component which is proportional to the DC magnetic to be measured. The sensor core is excited by a square waveform of voltage through the excitation coil of 80 turns. The second harmonic output of pick-up coil(x and y axis: 100 turns) is measured by FFT spectrum analyzer. This result is compared with output of PSD(phase sensitive detector) unit for detecting the second harmonic component. The measured maximum sensitivity is about 1580 V/T at driving frequency of 1.5 kHz and excitation current of 2 App. The nonlinearity of this system is measured about 2.3%(PSD) and about 1%(second harmonics of the pick-up). The angle error of the system is ${\pm}2$ %/FS.

• Applied Microscopy
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• v.34 no.1
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• pp.13-21
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• 2004

The high voltage electron microscope (HVEM) newly installed in KBSI is an advanced transmission electron microscope capable of atomic resolution (${\leq}1.2{\AA}$ point-to-point resolution) together with high titling function (${\pm}60^{\circ}$), which are suitable to do 3-dimensional atomic imaging of a specimen. In addition, the instrument can be controlled by remote operation system, named as 'FasTEM' for the HVEM, which is favorable to overcome some environmental obstacles resulting from the direct operation. The FasTEM remote operation system has been established between the headquarter of KBSI in Daejeon and the Seoul branch. The server system in the headquarter has been connected with a portable client console system in the Seoul branch using an advanced internet resource, 'KOREN' of 155 Mbps grade. Most of the HVEM functions essential to do remote operation are available on the portable client console. The experiment to acquire the high resolution image of [001] Au has been achieved by excellent transmission of control signals and communication with the HVEM. Real-time reaction like direct operation, such as controls of the illumination and projection parameters, acquisition and adjustment of each detector signal, and electrical steering of each motor-driven system has been realized in remote site. It is positively anticipated that the first remote operation of HVEM in conjunction with IT infraengineering plays a important role in constructing the network based e-Science Grid in Korea for national user s facilities.