• Microbiology and Biotechnology Letters
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• v.33 no.2
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• pp.136-141
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• 2005

A bench-scale feasibility study was conducted with solid farming feed to evaluate a treatment process for microbiological removal of nitrogen (N) and phosphorus (P). Strains, Bacillus sp. CK-10 and Bacillus sp. CK-13, were originally isolated from water samples of shrimp farming pond. Simultaneous removal of N/P in marine media was monitored in the co-cultures, CK-10 and CK-13. As the results, $400\;{\mu}M\;NH^{+}_4$ and $400\;{\mu}M\;NO^{-}_2$ were eliminated within 12 hours and $NO^{-}_3$ within 36 hours, and $500\;{\mu}M\;PO^{-3}_4$ was completely disappeared within 36 hours from the media. Cultures of CK-10 and CK-13 were applied for removal of N/P leached from shrimp farming fred. HPAEC-PAD system was used to analyze sugars in farming feed, resulting in resolution of various sugars including glucose, galactose, galatosamine, mannose, and fucose. $0.2\%$ (w/v) Pulp densities of the farming feed contained approximately $33.3\;{\mu}M\;NH^{+}_4,\;12.9\;{\mu}M\;NO^{-}_2.\;81.5\;{\mu}M\;NO^{-}_3\;and\;248\;{\mu}M\;PO^{-3}_4$ which could dissolved within 72 hours of leaching in aqueous solution followed by bacterial removal. Complete bacterial removal of N/P was achieved within 84 hours at $0.2\%$ of the feed in co-cultures, whereas single cultures removed to incompletion of N/P during the incubation period. This work demonstrated that test cultures, CK-10 and CK-13 showed effective removal of N/P derived from shrimp farming feed.

• Journal of Environmental Impact Assessment
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• v.18 no.6
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• pp.461-467
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• 2009

This paper provides two case studies of environmental impacts with socio-economic values. The first case is on flood protection levees conducted from 2003 to 2004 in Phu Tan district, An Giang province. The impacts were found by comparing full flood protection levees area (FFPL) to non-full flood protection levees area (NFFPL). Participatory Rural Appraisal (PRA) tools per each group of rich, middle, and poor people were used to list the impacts. Then, major impacts were selected by ranking and interviewing 60 households per site, and assessed by Cost Benefit Analysis (CBA) in rice production from 1996 to 2002 between two areas. The tested research indicated moving system of NFFPL to that of FFPL lost about 11 million VND/ha/year. The second case is on impacts of Penaeid shrimp farming conducted in Duyen Hai District, Tra Vinh Province in 2004-2005. Ninety households and 12 local officials were interviewed. Four PRAs were conducted and 36 water samples were taken inside and outside shrimp pond to measure values of DO, COD, Fe total, TSS, N-$NO_3{^-}$, N-$NH_4{^+}$, P-$PO{_4}^{3-}$, and Chlorophyll-a. Research results showed only 36.7% of the households got profit from shrimp farming. Highest financial efficiency was 0.72 for the semi-intensive system. Tested water indicators showed surface water quality did not match Vietnamese standard for surface water in coastal area (TCVN 5943-1995) and in rain. The water was very muddy and contaminated by organic aluminum. Summarily, the impacts were clarified more obviously via adding socio-economic values to assessment. Importantly, the values were transformed to household's income which is an indicator for policy-makers to consider the impacts obviously. Besides, data of different group of people impacted are cases contributing to consideration of the impacts in an appropriate social level.

• Journal of Microbiology and Biotechnology
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• v.29 no.7
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• pp.1104-1116
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• 2019

In this study, we investigated the potential of using sediment bioelectrochemical systems (SBESs) for in situ treatment of the water and sediment in brackish aquaculture ponds polluted with uneaten feed. An SBES integrated into a laboratory-scale tank simulating a brackish aquaculture pond was established. This test tank and the control (not containing the SBES) were fed with shrimp feed in a scheme that mimics a situation where 50% of feed is uneaten. After the SBES was inoculated with microbial sources from actual shrimp pond sediments, electricity generation was well observed from the first experimental week, indicating successful enrichment of electrochemically active bacteria in the test tank sediment. The electricity generation became steady after 3 weeks of operation, with an average current density of $2.3mA/m^2$ anode surface and an average power density of $0.05mW/m^2$ anode surface. The SBES removed 20-30% more COD of the tank water, compared to the control. After 1 year, the SBES also reduced the amount of sediment in the tank by 40% and thus could remove approximately 40% more COD and approximately 52% more nitrogen from the sediment, compared to the control. Insignificant amounts of nitrite and nitrate were detected, suggesting complete removal of nitrogen by the system. PCR-DGGE-based analyses revealed the dominant presence of Methylophilus rhizosphaerae, Desulfatitalea tepidiphila and Thiothrix eikelboomii, which have not been found in bioelectrochemical systems before, in the bacterial community in the sediment of the SBES-containing tank. The results of this research demonstrate the potential application of SBESs in helping to reduce water pollution threats, fish and shrimp disease risks, and thus farmers' losses.

• Animal Systematics, Evolution and Diversity
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• v.7 no.2
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• pp.233-240
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• 1991

The morphology and ecology of the marine hypotrichous ciliate, Oxytricha saltans (Cohn, 1866) Kahl, 1932 collected from shrimp culturing pond of Qingdao (Tsingtao, 36$^{\circ}$08'N : 120$^{\circ}$43'E) , P.R. China were investigated. collected samples were observed directly and we also observed cultured ones and stained specimens. In relation to this species were described the morphology and infraciliature in detail using protargol method, presented a new diagnosis and discussed some synonyms and the related species, Actinotricha saltans sensu Dragesco, 1963.

• Journal of Aquaculture
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• v.22 no.1
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• pp.42-50
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• 2009

Shrimp farming which is entirely conducted in outdoor ponds in the west coast of Korea has been suffered from mass mortality due to viral epizootics. Intensive indoor shrimp culture under limited water exchange can solve these problems of outdoor ponds including viral transmission from environment, pollution due to discharge of rearing water, low productivity and limited culture period. In this study, juvenile L. vannamei (B.W. 0.08-0.09 g) was stocked with $3,000-5,455/m^3$ in density in four raceway tanks (two $12.9\;m^2$, two $18\;m^2$ tanks) and cultured for 42 days with 2.7-3.4% of daily water exchange. Results from four tanks showed FCR of 0.79-1.29, survival of 38.2-48.0%, and yields of $2.49-4.22\;kg/m^3$ which is consistent with 12-20 and 8-14 times higher than those of commercial shrimp hatchery and outdoor pond in Korea, respectively. Concentrations of total ammonia nitrogen in all four tanks were 1.11-1.42 ppm in mean level and did not exceed 6.0 ppm (0.096 ppm of $NH_3$) which is still acceptable levels for shrimp growth. During the culture trial, concentration of $NO_2$-N rapidly increased from stocking, resulting in mean concentration of 18.45-22.07 ppm. It also exceeded 10 ppm over four weeks and maintained at 35-45 ppm for four days in all tanks, accounting for low survival of shrimp due to long-term exposure to high concentration of $NO_2$-N. Nevertheless, the results with survival rate over 38% from raceways which experienced the extreme $NO_2$-N levels suggests that under "biofloc system" white shrimp can acclimate to high $NO_2$-N concentration to some degree.

• Korean Journal of Fisheries and Aquatic Sciences
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• v.5 no.2
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• pp.45-49
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• 1972

The following are some results obtained from a series of experiments in rotifer culture and its usage for the food of tiny fish fry: 1, Outdoor concrete ponds, each being $16m^2$, were used to culture the rotifers, Brachionus calyciflorus, and Filinia longiseta. Brachionus calyciflorus usually attained the population of about 100 individuals per ml of pond water. Dipterex was usually applied to control Daphni,a and other crustaceans that generally appear and feed on rotifers. A concentration of 0.16 to 0.2 ppm in the pond water was sufficiently effective to control these natural enimies of rotifers. Poultry dung was very effectively used to multiplicate rotifers. The fertilization ratio was about 8 kg each pond with 30cm depth of water. 2. The tiny rotifer, Filinia longiseta attained a very high population density of about 1,000 individuals per ml of pond water, but they were very sensitive to dipterex, and for this aspect future investigation may be needed. 3. In the outdoor ponds, the multiplication of rotifers significantly decreased when the water temperature falls to about $20^{\circ}C$ in autumn. 4. In the laboratory room, unicellular planktonic algae such as Scenedesmus or Chlorella, as the food of rotifers, were collected from the outdoor ponds by dipping them together with water, and were effectively used for the culture of Brachionus calyciflorus. If the planktonic algae are cultured in specially designed containers, the sun-light would be the most effective means as the source of light. 5. Brachionus calyciflorus cultured in the outdoor ponds by the dipterex controlled method was highly efficient to rear the early fry of marble gourami. The dipterex content mixed in the water to control the crustacean emmies of rotifers sieved no harm to the gourami fish fry.

• KSBB Journal
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• v.20 no.2 s.91
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• pp.116-122
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• 2005

The feasibility of using bacterial cultures with the ultimate aim for the marine environmental clean-up was explored. The present study reports on the bacterial elimination of nitrogens and phosphorus by strains CK-10 and CK-13 isolated from shrimp farming pond. The strains were identified as genus Bacillus on the basis of BIOLOG test, and designated as Bacillus sp. CK-10 and Bacillus sp. CK-13, respectively. Removal of nitrogens $(NH_4^+,\;NO_2^-,\;or\;NO_3^-)$ or phosphorus $(PO_4^{-3})$ as single N or P source was studied with single cultures under aerobic conditions. Complete elimination of all nitrogens in the concentration range of $100-400{\mu}M$ was achieved in single cultures as well as co-cultures within the given incubation period. Similar results were obtained from the test cultures containing $125-599{\mu}M\;PO_4^{3-}$. Simultaneous removal of all N/P was monitored in the co-cultures. As the results, $400{\mu}M\;NH_4^+\;and\;NO_2^-$ were eliminated within 12hrs and $400{\mu}M\;NO_3^-\;and\;500{\mu}M\;PO_4^{-3}$ were completely disappeared within 36 hrs from the media. The work demonstrated that co-cultures improved the concurrent removal of N/P from the media.

• Korean Journal of Microbiology
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• v.41 no.1
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• pp.18-23
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• 2005

The purpose of this work was to investigate the physiological characteristics of Lactobacillus sp. JK-8 isolated from a shrimp aquaculture pond. The strain JK-8 was grown on MRS media, and morphological and physiological characteristics of the strain were examined. The bacterium was identified as a strain of the genus Lactobacillus on the basis of BIOLOG test. Strain JK-8 produced both lactic acid and acetic acid, which were responsible for the pH decrease during growth. Concentrations of lactic acid and acetic acid increased to 192.8 mM and 43.6 mM, respectively, and the initial pH 7.0 of the cultures decreased to 3.8 at the end of incubaction. The bacteriocidal effect against eight target bacteria was examined with 5-fold concentrated culture supernatants. All bacteria tested in this work were completely killed within 3 hrs after treatment with the culture supernatant. The bacteriocidal effects were clearly observed, only when the pH of the culture supernatants were not adjusted. HPLC was used to reslove lactic acid and acetic acid in the culture solution, and GC-MS was used to verify the metabolites.

• Journal of the East Asian Society of Dietary Life
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• v.7 no.4
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• pp.512-518
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• 1997

This study was performed to determine the amount of aluminum, which is one of the factors of Alzheimer's disease, In some fishes caught from some areas of the west coast in Korea. The 46 aquatic products were composed of fishes, molluscs, and salt-fermented products (jeot-gal). The 24 fishes were Hickory shad, Gobies, Pomfref, Atkafish, Flounder, Jambeng-ie Monk fish, Yellow hair tail, Mackerel, Bartailed flathead, Alaska pollack, Brown croaker, Eel, Fine-spotted flounder, Black spotted grouper, Sea-eel, Pacific saury, Areliscus honaleus, Small boil-dried anchovy, Croaker, Hair tail, Sea bream genuine, Motleystrip rainbowfish, and Bastard halibut. The 15 Molluscs were Whip-arm octopus, Sea arrow, Common squid, Han chi, Cuttle fish, Turban shell, Pond snail, Orient calm, Surf calm, Butter calm, Crib shell, Oyster, Egg cockle, Little neck calm, and Arkshell. The 7 salt-fermented products were salt-fermented Shrimp, Little neck, Oyster, Shad, Gonjeng-ie, Hqangsegi, and Squid. All of them were ashed with 5$m\ell$ HNO$_3$ and then with 10$m\ell$ ternary solution (HNO$_3$ : H$_2$SO$_4$ : HClO$_4$= 10 : 1 : 4). After ashing of the samples, the aluminum amount were measured by ICP. The aluminum amount of molluscs was significantly higher than that of fishes and salt-fermented products(p＜0.01). The aluminum amount of Orient calm and Healak in molluscs were 827.70, 812.55ppm, respectively, which were the most amounts compared nth that of the other samples. But the aluminum amounts of Bartailed flathead and Sea bream, genuine In fishes were 0.98, 0.97ppm, respectively, which were the least amounts compared with that of other samples. This study was limited within 46 aquatic samples, therefore I hope there will be wider efforts to determine about auminum amount in broade range of aquatic foods for the prevention of Alzheimer's disease.