• Journal of Distribution Science
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• v.19 no.4
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• pp.53-60
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• 2021

Purpose: This study focuses on exploring ways to improve the distribution method of shrimp farming so that it is eco-friendly and increases the distribution of shrimp. Research design, data and methodology: The experimental device installed in a biofloc shrimp culture in one area tested 10 times. Complex odor, concentration of H2S, water quality improvement effected by decomposition of organic substances, and degree of microbial activation measured. The data of the experimental results verified using the T-test technique, and the p value was determined based on the significance probability of 0.05. Results: This experimental device was effective in reducing odor and hydrogen sulfide in shrimp farms. With the improvement of water quality, dissolved oxygen increased due to the microbubble and cavitation action of air ejector and ultrasonic waves. In addition, the cultured microorganisms in the cultured water treated by the experimental device were remarkably proliferated compared to the raw water. Conclusions: The biofloc distribution method has a significant effect on improving water quality and reducing odor substances and will become a new eco-friendly and efficient distribution method for shrimp farming in the future.

• Journal of Environmental Impact Assessment
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• v.18 no.6
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• pp.461-467
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• 2009

This paper provides two case studies of environmental impacts with socio-economic values. The first case is on flood protection levees conducted from 2003 to 2004 in Phu Tan district, An Giang province. The impacts were found by comparing full flood protection levees area (FFPL) to non-full flood protection levees area (NFFPL). Participatory Rural Appraisal (PRA) tools per each group of rich, middle, and poor people were used to list the impacts. Then, major impacts were selected by ranking and interviewing 60 households per site, and assessed by Cost Benefit Analysis (CBA) in rice production from 1996 to 2002 between two areas. The tested research indicated moving system of NFFPL to that of FFPL lost about 11 million VND/ha/year. The second case is on impacts of Penaeid shrimp farming conducted in Duyen Hai District, Tra Vinh Province in 2004-2005. Ninety households and 12 local officials were interviewed. Four PRAs were conducted and 36 water samples were taken inside and outside shrimp pond to measure values of DO, COD, Fe total, TSS, N-$NO_3{^-}$, N-$NH_4{^+}$, P-$PO{_4}^{3-}$, and Chlorophyll-a. Research results showed only 36.7% of the households got profit from shrimp farming. Highest financial efficiency was 0.72 for the semi-intensive system. Tested water indicators showed surface water quality did not match Vietnamese standard for surface water in coastal area (TCVN 5943-1995) and in rain. The water was very muddy and contaminated by organic aluminum. Summarily, the impacts were clarified more obviously via adding socio-economic values to assessment. Importantly, the values were transformed to household's income which is an indicator for policy-makers to consider the impacts obviously. Besides, data of different group of people impacted are cases contributing to consideration of the impacts in an appropriate social level.

• Microbiology and Biotechnology Letters
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• v.33 no.2
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• pp.136-141
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• 2005

A bench-scale feasibility study was conducted with solid farming feed to evaluate a treatment process for microbiological removal of nitrogen (N) and phosphorus (P). Strains, Bacillus sp. CK-10 and Bacillus sp. CK-13, were originally isolated from water samples of shrimp farming pond. Simultaneous removal of N/P in marine media was monitored in the co-cultures, CK-10 and CK-13. As the results, $400\;{\mu}M\;NH^{+}_4$ and $400\;{\mu}M\;NO^{-}_2$ were eliminated within 12 hours and $NO^{-}_3$ within 36 hours, and $500\;{\mu}M\;PO^{-3}_4$ was completely disappeared within 36 hours from the media. Cultures of CK-10 and CK-13 were applied for removal of N/P leached from shrimp farming fred. HPAEC-PAD system was used to analyze sugars in farming feed, resulting in resolution of various sugars including glucose, galactose, galatosamine, mannose, and fucose. $0.2\%$ (w/v) Pulp densities of the farming feed contained approximately $33.3\;{\mu}M\;NH^{+}_4,\;12.9\;{\mu}M\;NO^{-}_2.\;81.5\;{\mu}M\;NO^{-}_3\;and\;248\;{\mu}M\;PO^{-3}_4$ which could dissolved within 72 hours of leaching in aqueous solution followed by bacterial removal. Complete bacterial removal of N/P was achieved within 84 hours at $0.2\%$ of the feed in co-cultures, whereas single cultures removed to incompletion of N/P during the incubation period. This work demonstrated that test cultures, CK-10 and CK-13 showed effective removal of N/P derived from shrimp farming feed.

• Journal of Sensor Science and Technology
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• v.30 no.3
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• pp.154-164
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• 2021

In this study, the IoT sensor technology required for improving the survival rate and high-density productivity of individual shrimp in smart shrimp farming (which involves the usage of recirculating aquaculture systems and biofloc technology) was analyzed. The principles and performances of domestic and overseas water quality monitoring IoT sensors were compared. Furthermore, the drawbacks of existing aquaculture monitoring technologies and the countermeasures for future aquaculture monitoring technologies were examined. In particular, for farming white-legged shrimp, an IoT sensor was employed to collect measurement indicators for managing the water quality environment in real-time, and the IoT sensor-based real-time monitoring technology was then analyzed for implementing the optimal farming environment. The results obtained from this study can potentially contribute to the realization of an autonomous farming platform that can improve the survival rate and productivity of shrimp, achieve feed reduction, improve the water quality environment, and save energy.

• Journal of the Korean Society of Fisheries and Ocean Technology
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• v.55 no.4
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• pp.310-319
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• 2019

Although mud shrimp (Upogebia Major) provides benefits on the tidal flat ecosystem, it is causing the mass mortality of Manila clam (Tapes philippinarum) and large economic loss to the aquaculture farmer due to spreading in the entire coastal clam farm area of the West Sea of Korea. In this study, a mud shrimp blocking box for cultivating clam was tested to prevent the mud shrimp from invading the habitat of the clam. To determine the effect of the blocking box, the growth characteristics of the clams under various culture conditions were investigated. The shell length, total weight, condition index, the presence of mud shrimp in the box, and the soil decay in the box were examined according to whether the clams were raised in the box or in natural state, whether the box is covered with a net or not and the gap size of the box. As a result, no significant differences in shell length, total weight, and condition index were found between the clam grown in the box and the natural state (P>0.05) without any evidence of invading of mud shrimp to the box. In addition, the soil inside the box was in good condition, not decayed. Therefore, using the mud shrimp blocking box, mud shrimp can be prevented from propagating to the habitat of the clam, thereby contributing to the normal production of the clam and thus to restoring the clam farming.

• Smart Media Journal
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• v.12 no.3
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• pp.112-119
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• 2023

Shrimp farming has been becoming a new source of income for fishermen in South Korea. It is often necessary for fishers to measure the size of the shrimp for the purpose to understand the growth rate of the shrimp and to determine the amount of food put into the breeding pond. Traditional methods rely on humans, which has huge time and labor costs. This paper proposes a deep learning-based method for calculating the size of shrimps automatically. Firstly, we use fine-tuning techniques to update the Mask RCNN model with our farm data, enabling it to segment shrimps and generate shrimp masks. We then use skeletonizing method and maximum inscribed circle to calculate the length and width of shrimp, respectively. Our method is simple yet effective, and most importantly, it requires a small hardware resource and is easy to deploy to shrimp farms.

• Fisheries and Aquatic Sciences
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• v.22 no.8
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• pp.17.1-17.9
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• 2019

Bacillus is a diverse genus consisting of more than 200 species with extensive genetic diversity. Their beneficial effects in industrial shrimp farming have been well documented. However, little is known about the biodiversity of the Bacillus spp. in this aquaculture system. Taxonomic analysis by 16S rRNA sequencing does not always allow species-level identification of Bacillus spp. In this study, 26 Bacillus isolates from two industrial Litopenaeus vannamei shrimp ponds in Bac Lieu Province, Vietnam, were analyzed for their genetic diversity by multi-locus sequence typing (MLST). A total of 22 sequence types were identified and segregated into four distinct clusters, corresponding to B. subtilis, B. velezensis, B. siamensis, and B. licheniformis. Bacillus subtilis and B. velezensis accounted for more than 73% of the Bacillus isolates. Notably, the MLST scheme exhibited high discriminatory power and might be further simplified to be a convenient method to identify species of the genus Bacillus.

• BMB Reports
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• v.37 no.6
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• pp.726-734
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• 2004

White spot disease (WSD) is caused by the white spot syndrome virus (WSSV), which results in devastating losses to the shrimp farming industry around the world. However, the mechanism of virus entry and spread into the shrimp cells is unknown. A binding assay in vitro demonstrated VP28-EGFP (envelope protein VP28 fused with enhanced green fluorescence protein) binding to shrimp cells. This provides direct evidence that VP28-EGFP can bind to shrimp cells at pH 6.0 within 0.5 h. However, the protein was observed to enter the cytoplasm 3 h post-adsorption. Meanwhile, the plaque inhibition test showed that the polyclonal antibody against VP28 (a major envelope protein of WSSV) could neutralize the WSSV and block an infection with the virus. The result of competition ELISA further confirmed that the envelope protein VP28 could compete with WSSV to bind to shrimp cells. Overall, VP28 of the WSSV can bind to shrimp cells as an attachment protein, and can help the virus enter the cytoplasm.

• Journal of Microbiology and Biotechnology
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• v.28 no.6
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• pp.946-956
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• 2018

The gut microbiota of aquatic animals plays a crucial role in host health through nutrient acquisition and outcompetition of pathogens. In this study, on the basis of the high-throughput sequencing of 16S rRNA gene amplicons, we examined the bacterial communities in the gut of freshwater shrimp (Macrobrachium nipponense) and in their living environments (sediment and pond water) and analyzed the effects of abiotic and biotic factors on the shrimp gut bacterial communities. High bacterial heterogeneity was observed in the freshwater shrimp gut samples, and the result indicated that both the surrounding bacterial community and water quality factors (particularly dissolved oxygen and temperature) could affect the shrimp gut bacterial community. Despite the observed heterogeneity, 57 genera, constituting 38-99% of the total genera in each of the 40 shrimp gut samples, were identified as the main bacterial population in the gut of M. nipponense. In addition, a high diversity and abundance of lactic acid bacteria (26 genera), which could play significant roles in the digestion process in shrimp, were observed in the shrimp gut samples. Overall, this study provides insights into the gut bacterial communities of freshwater shrimp and basic information for shrimp farming regarding the application of probiotics and disease prevention.

• Korean Journal of Fisheries and Aquatic Sciences
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• v.43 no.4
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• pp.331-339
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• 2010

Shrimp farming is the most important mariculture industry on the west coast of South Korea. However, it has suffered from mass mortality due to viral disease outbreaks and coastal pollution due to water discharge. This study developed an intensive shrimp culture method for outdoor ponds, without water exchange, which minimizes the chance of viral transmission from the environment, reduces coastal pollution by water discharge and enhances shrimp production. A culture trial was conducted in two high-density polyethylene (HDPE)-lined ponds with a $550\;m^2$ surface area. The ponds were stocked with postlarvae of Litopenaeus vannamei, the major farmed shrimp species in Korea, on July 10, 2007, and cultured for 90 days with no water exchange. The stocking density of the postlarvae (B.W. 0.0015 g) was $272\;ind./m^2$, which is eight times higher than in traditional pond culture in Korea. At harvest, the total production of ponds 1 and 2 was 1,362kg ($2.48\;kg/m^2$) and 1,282 kg ($2.33\;kg/m^2$), respectively. This is 20~22 times higher than the mean farmed shrimp production ($0.112\;kg/m^2$) in Korea and about eight times higher than in traditional ponds with a good harvest. Although there was no water exchange throughout the culture period, the mean concentrations of unionized ammonia and nitrite-nitrogen were as low as 0.038 and 6.0 mg/L, respectively. The feed conversion rate (FCR) was 1.38, which is 20~45% lower than that of traditional pond cultures. The high efficiency of the diet in this study is thought to be due to a well-managed feeding strategy and well-developed bioflocs used as diet additions for the shrimp. The final body weight of the shrimp at harvest was low (12.2~12.5 g), compared with that of traditional pond culture. This may have resulted from the combination of a short culture period, high density of shrimp, and low temperature. This study suggests that a super-intensive shrimp pond culture method using biofloc technology with no water exchange can minimize viral transmission via water exchange, reduce coastal pollution, and enhance shrimp production.