• 한국수정란이식학회지
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• 제29권3호
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• pp.221-228
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• 2014

Despite that porcine spermatogonial stem cells (pSSCs) have been regarded as a practical tool for preserving eternally genetic backgrounds derived from pigs with high performance in the economic traits or phenotypes of specific human diseases, there were no reports about precise definition of niche conditions promoting proliferation and maintenance of pSSCs. Accordingly, we tried to determine niche conditions supporting proliferation and maintenance of undifferentiated pSSCs for short-term. For these, undifferentiated pSSCs were progressively cultured in different composition of culture medium, seeding density of pSSCs, type of feeder cells and concentration of growth factors, and then total number of and alkaline phosphatase (AP) activity of pSSCs were investigated at post-6 day culture. As the results, the culture of $4{\times}10^5$ pSSCs on mitotically in activated $2{\times}10^5$ STO cells in the mouse embryonic stem cell culture medium (mESCCM) supplemented with 30 ng/ml glial cell line-derived neurotrophic factor (GDNF) was identified as the best niche condition supporting effectively the short-term maintenance of undifferentiated pSSCs. Moreover, the optimized short-term culture system will be a basis for developing long-term culture system of pSSCs in the following researches.

• Clinical and Experimental Reproductive Medicine
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• 제47권4호
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• pp.312-318
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• 2020

Objective: The objective of the study was to compare the effects of long-term and short-term embryo culture to assess whether there is a correlation between culture duration and clinical outcomes. Methods: Embryos were divided into two study groups depending on whether their post-warming culture period was long-term (20-24 hours) or short-term (2-4 hours). Embryo morphology was analyzed with a time-lapse monitoring device to estimate the appropriate timing and parameters for evaluating embryos with high implantation potency in both groups. Propensity score matching was performed to adjust the confounding factors across groups. The grades of embryos and blastocoels, morphokinetic parameters, implantation rate, and ongoing pregnancy rate were compared. Results: No significant differences were observed in the implantation rate or ongoing pregnancy rate between the two groups (long-term culture group vs. short-term culture group: 56.3% vs. 67.9%, p=0.182; 47.3% vs. 53.6%, p=0.513). After warming, there were more expanded and hatching/hatched blastocysts in the long-term culture group than in the short-term culture group, but there was no significant between-group difference in embryo grade. Regarding pregnancy outcomes, the time to complete blastocyst re-expansion after warming is shorter in women who became pregnant than in those who did not in both culture groups (long-term: 2.19±0.63 vs. 4.11±0.81 hours, p=0.003; short-term: 1.17±0.29 vs. 1.94±0.76 hours, p=0.018, respectively). Conclusion: The outcomes of short-term culture and long-term culture were not significantly different in vitrified-warmed blastocyst transfer. Regardless of the post-warming culture time, the degree of blastocyst re-expansion 3-4 hours after warming is an important marker for embryo selection.

• 국제교류와 융합교육
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• 제3권1호
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• pp.87-103
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• 2023

최근 미디어 시대에 짧은 영상의 발전 추세가 강하게 나타났다. 이러한 추세는 단편 영상에 대해 급진적인 발전과 동시에 혁신적인 문화의 표현 방식을 통해 중국의 우수한 전통 문화의 전파 발전을 위한 새로운 기회를 가져왔다. 이러한 환경 속에서 영상 블로커 이쯔치는 같은 유형의 셀프 미디어를 업로드함으로써 인기를 짧은 시간 내에 얻을 수 있었다. 이는 개인 매체 시대에 중화 전통 문화의 재구성과 전파하는 방법을 분석하는 데 좋은 소재를 제공했다. 본 연구는 유명한 동영상 블로커인 이쯔치의 사례를 연구하여 이를 바탕으로 셀프 미디어 시대 중국 전통 문화의 재구성과 전파에 대해 논하고자 한다. 그러기 위해 셀프 미디어 시대에 전통 문화의 재구성과 전파 방식에 대한 내용 분석을 하였다. 그 결과 현재 전통 문화 전파에 존재하는 문제점을 밝히고 보다 전통 문화를 전파할 수 있는 최적화된 전략을 제시하였다.

• 한국의상디자인학회지
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• 제1권1호
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• pp.17-34
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• 1999

Because the United States of America has a short history, they don‘t have a special traditional culture or costumes. Besides, America is a multi-people country, they couldn’t have a consistent culture or a traditional costumes. But even they have a short history, they are trying to make a new culture. America is searching for a representative culture, which are like festival costumes, regional costumes and costumes that are form the group of people, in there country and making it into their a convention culture. This thesis is about the costumes of the U.S.A.'s famous festivals, traditional and festival costumes that are differ from every other states and the traditional costume that they are making it righ

• 복식문화연구
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• 제12권2호
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• pp.224-238
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• 2004

This paper analyzes the similarity of fashion in the 1920's and 1960's. Fashion is a reflection of Zeitgeist. The similarity of fashion appeared in the similar social ideal period. The fashion of the 1920's and 1960's have a lot of similarity in many respects. Androgyny was the new word. The woman cut her hair short, wore short skirt, and projected a self-confidence that was considered by many to be too masculine. The new fashions also appeared very youthful. The cult of eternal youth was born. The mature woman was no longer requested. Instead, in the face of changing lifestyles and extremely rapid technological development, taste ran in favor of a young, athletic, and mobile ideal. The new fashions do-emphasized curvaceous shapes through short dresses and short hair-both styles were supposed to express youthfulness. Characteristic of the times was the short loose dress: straight silhouette disguised feminine curves. The new dresses were invented for very young, slim, and wide-eyed women. The common Zeitgeist of the 1920's and 1960's represents the cult of youth and the adoption of innovative style, which emphasized decoration-cleared simplicity, functionality, practicality, activity, androgyny. Innovative short skirt, youth fashion, androgynous style, unisex style, tubular silhouette, short hair style, and eyeline-emphasized makeup were analyzed by the similarity of fashion in the 1920's and 1960's.

• 한국동물생명공학회지
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• 제38권3호
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• pp.177-187
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• 2023

Background: The grading of Hanwoo (Korean native cattle) is based on four economic traits, and efforts have been continuously made to improve the genetic traits associated with these traits. There is a technology to predict the expected grade based on the 4 economic genetic SNP characteristics of Korean cattle calves using single nucleotide polymorphism (SNP) technology. Selection of highly proliferative, self-renewing, and differentiating satellite cells from cattle is a key technology in the cultured meat industry. Methods: We selected the Hanwoo with high and low-scored of genomic estimated breeding value (GEBV) by using the Hanwoo 50K SNP bead chip. We then isolated the bovine satellite cells from the chuck mass. We then conducted comparative analyses of cell proliferation, immunocytochemistry, qRT-PCR at short- and long-term culture. We also analyzed the differentiation capability at short term culture. Results: Our result showed that the proliferation was significantly high at High scored GEBV (Hs-GEBV) compared to Low scored GEBV (Ls-GEBV) at short- and long-term culture. The expression levels of Pax3 were significantly higher in Hs-GEBV bovine satellite cells at long-term culture. However, there were no significant differences in the expression levels of Pax7 between Hs- and Ls-GEBV bovine satellite cells at short- and long- term culture. The expression levels of MyoG and MyHC were significantly high at Ls-GEBV bovine satellite cells. Conclusions: Our results indicated that selection of bovine satellite cells by Hanwoo 50K SNP bead chip could be effective selection methods for massive producing of satellite cells.

• 복식문화연구
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• 제23권3호
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• pp.512-522
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• 2015

The aim of this study was to investigate the change in women's somatotype with aging. The subjects were 1,123 women aged 40~69. Their anthropometric data were from the 6th Size Korea. The data were analyzed by factor analysis and cluster analysis. Seven factors were extracted: body mass, body length, back shoulder, arm length factor, front interscye factor, body rise factor, and shoulder angle. The upper body types of middle-aged and elderly women were classified into five types: skinny, short stout body type with forward posture, composite, tall & full body type, and short & skinny. The skinny and composite body type appeared more often than the short stout body type in the early 40s of Korean women. Starting in the mid-50s, composite body type was less often found. However, the number of women with short stout body type increased. In the 60s, the number of women with short stout and tall & full body types decreased. These results reveal that the body types of middle-aged and elderly women changed with some pattern with aging. And women in their early 40s, mid-50s, and 60s women had different body shapes and postures.

• 만화애니메이션 연구
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• 통권20호
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• pp.15-29
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• 2010

문화예술교육은 전 국민의 문화예술향유를 통한 삶의 질 향상과 국가의 문화 역량 강화를 지향하는 교육으로서, 지금까지 다양한 프로그램들을 통해 그 범위가 확산되어 왔다. 그러나 실제로 학교수업을 통해 진행되는 학교문화예술교육은 표면적이고 기능적인 교육에 그치는 등 수업 내용과 방식에서 한계를 보이고 있다. 학교문화예술교육이 원래의 목적을 달성하고 학교 안에서 체계적이고 안정적인 부분으로 자리 잡기 위해서는 양적인 성장에 더하여 질적 심화의 측면을 고려해야 할 시점에 와있다. 이 논문에서는 학교문화예술교육의 질적 제고와 활성화를 위해, 초등학교 교육과정에서 한국단편애니메이션을 활용해서 기존 교과와 문화예술교육을 통합할 수 있는 문화예술통합교육 모형을 개발하였다. 단편애니메이션은 감독 개개인의 세계관과 개성, 개인의 삶과 사회적 관계 및 역사적 성찰에 이르기까지 다양한 주제를 독창적인 미학으로 표현해내고 있어, 풍부한 미적 경험과 더불어 주제중심통합교육을 위한 단초를 제공한다. <오늘이>(2003)는 제주도의 원천강 본풀이라는 신화적 소재와 민화라는 전통적 소재를 현대적으로 재해석한 작품이다. 이 작품은 국어(언어지능)와 도덕(대인/자성지능)을 통합적으로 구성하여 자신의 생각을 글, 만화, 그림으로 표현하게 함으로써 언어지능과 공간지능을 발휘할 수 있다. 이러한 문화예술통합교육 모델을 통해 개별교과 로 이루어지는 분과교육과정의 한계를 극복하여 정규 교과의 경계를 확장해서 전반적인 교육의 질을 개선하고자 한다.

• International Journal of Advanced Culture Technology
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• 제5권4호
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• pp.57-62
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• 2017

In this paper, we applied long short term memory(LSTM) for classifying political polarity in cyber public sphere. The data collected from the cyber public sphere is transformed into word corpus data through word embedding. Based on this word corpus data, we train recurrent neural network (RNN) which is connected by LSTM's. Softmax function is applied at the output of the RNN. We conducted our proposed system to obtain experimental results, and we will enhance our proposed system by refining LSTM in our system.

• 복식문화연구
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• 제6권4호
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• pp.13-25
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• 1998

This study focuses on the visual effects of various neckline-haristyle combinations on the perception of face image and type. The study employed a model with oval face and examined 35 combinations made up of five necklines and seven hairstyles. Looking at various face images depending upon different necklines, in case of round, V, boat square, and high necklines, long wave hair and medium wave hair produced a feminine image of marked individuality; long straight hair that covers the forehead and medium straight hair gave an image of charm and purity as well as an image of neatness; and long straight hair short cut hair showed an intellectual image. Regarding the perceptual type of face depending upon the different necklines of round, V, square, and high, the long straight hair covering the forehead and medium wave hair had the effect of an optical illusion that made the face look short and round; and short cut hair made the face line look distinct and the face look oval and slender.