• 한국환경농학회지
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• 제22권4호
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• pp.294-299
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• 2003

제주도에서 자생하는 갯쑥부쟁이(Heteropappus hispidus (Thunb.))에 감마선을 10, 20, 40, 80, 120, 160, 200, 300, 400 Gy 수준으로 나누어서 조사한 뒤 발아율 및 발아 30일 후의 본엽 출현율, $LD_{50}$, 본엽길이, 줄기부분의 안토시아닌 색소 형성 유무, 생육 60일 후의 로젯형 엽, multi-shoot 형성율, 초장, 개화 결실률 등의 주요 양적형질들을 생존개체를 대상으로 실시하였다. 120Gy 까지의 발아율은 88.5%로 대조구와 큰 차이를 보이지 않았으나 160Gy 이상에서부터는 급격히 감소하여 치사선량($LD_{50}$)은 160 Gy 범위 정도였다. 또한, 본엽 출현율과 그 길이는 조사선량이 높아질수록 억제되었다. 안토시아닌 색소 형성은 조사선량이 높을수록 대조구 2%에서 30%로 높아졌으며 로젯형도 고선량 처리구에서 발생하였다. Multi-shooting은 80Gy 이상일 때부터 나타났으며 multi-shooting을 형성하는 개체들은 초장은 짧고 줄기는 가늘게 다발형으로 자라는 특성이 있어서 만일 형질이 고정된다면 화분 관상용 개체로 선발하기에 적합하였다. 특히, 조사선량이 증가됨에 따라서 안토시아닌 색소 형성, 로젯형 개체, multi-shoot등의 개체가 많이 출현되었는데 이는 돌연변이 유기의 선발지표가 될 수 있을 것으로 보인다.

• 한국자원식물학회:학술대회논문집
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• 한국자원식물학회 2003년도 춘계 학술발표대회
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• pp.131-132
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• 2003

Leaf discs and apical meristems were cultured in Murashige and Skoog (MS) medium supplemented with cytokinin and auxin at different concentrations. Callus production was observed in all tested media after six days of incubation. Callus produced in the presence of high concentration of NAA (2.0mg/1) was fragile in texture and yellow in colour. Highest callus formation was observed from leaf discs in the medium supplemented with 1.0mg/1 NAA and 0.5 mg/l BAP in dark at $25{\pm}1{\circ}C$. Percentage of callus formation was 95% and mean callus fresh weight was 654.88 43.53 mg. Shoots were induced from the callus after 4 weeks in 1/2MS medium supplemented with BAP and kinetin both at 0.5mg/1. When elongated shoots were separated and transferred into multiplication medium (MS+0.5mg/1 BAP+0.5mg/1 kinetin) multiplication rate was 6.4 after 6 weeks. Higher concentrations of BAP caused callus production at the base. Direct shoot induction was observed from apical meristems in MS medium in the presence of 0.175 mg/1 IAA + 2.25mg/1 BAP and 0.175 mg/1 IAA + 3.0 mg/1 BAP in 16 hour day at $25{\pm}1{\circ}C$. Explants (apical meristems) elongated to form a single shoot forming a callus at the base. Adventitious buds were sprouted out from the base. Percentage explants which producing shoots was 28.57 and 65.5 respectively. Multiple shoot induction was also observed in the same media. Highest multiple shoot production was observed in the presence of 0.175 mg/l IAA and 3.0mg/l BAP, Mean number of shoots per explant was 5.36 and the mean shoot length was $16.66{\pm}4.15$mm. Shoots (20 30m length) were tested for root induction. Excised shoots were transferred into rooting media, which contains different concentrations of NAA and IAA. Best rooting performance was observed in 1/2MS medium supplemented with 0.1mg/1 NAA after 10 days of incubation in 16 hr photoperiod at $25{\pm}1{\circ}C$. Mean number of roots per shoot was 6 and the mean root length was 252mm. Rooted plantlets were transferred into sterile coir dust:sand (1:4) mixture and maintained in a humid chamber for two weeks, They were gradually exposed to the natural environment. After three weeks they were transferred to pots containing coir dust:sand (1:2) mixture for further development where the 90% survival was observed.

• Journal of Plant Biotechnology
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• 제45권2호
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• pp.140-145
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• 2018

Inducing genetic and morphological variation through conventional method is very difficult. Therefore, mutation induction through in vitro technology brings numerous advantages over the conventional breeding. Thus, the individual shoots (1 ~ 2 cm) were irradiated with gamma rays (10 ~ 70 Gy). The result revealed that the explants treated with higher doses (40, 50, 60, and 70 Gy) showed deleterious effects of ionizing radiation. The highest survival rate among ${\gamma}$ treated explants recorded was 71% in 10 Gy treatments while the lowest survivality was 15% in 70 Gy. Lethal dose 50% ($LD_{50}$) dose was found to be 33 Gy. In the in vitro condition, rooting reponse showed that increase in gamma irradiation dose resulted in the inhibition of root growth. Meanwhile, non-treated explants had the best rooting ability with the maximum number of root per explant (20) within a short period of time (6 days), with the highest root length of (15.1 cm). The longer period in rooting (12 days) and lowest number of root per explant (8) with shortest root length (10.1 cm) were recorded at 30 Gy treatment. The highest shoot length (13.6 cm) was observed at control treatment and the shortest shoot length (10.4 cm) was observed at 30 Gy. In the nursery, lowest leaf number (5) was observed at 30 Gy compared with other treatments. The highest chlorophyll content (49.8) was recorded at 10 Gy treated seedling. Irradiated explants with 10 Gy found to be superior over the control treatment and had positive effects in main growth parameters such as chlorophyll content.

• Ecology and Resilient Infrastructure
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• 제9권4호
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• pp.259-268
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• 2022

본 연구는 장기간 중금속 오염에 노출된 폐 석탄매립지에 생육하고 있는 참김의털 (Festuca ovina var. coreana) 종자를 채집하고 발아한 유식물을 가지고 온실 재배 실험을 통해 생육특성과 중금속축적능을 평가하고 식물정화법 (phytoremediation) 적용 가능성을 위해 수행하였다. 참김의털을 온실에서 인공적으로 오염된 토양에서 12 주 동안 재배하였다. 비소처리 인공토양의 농도구배는 각각 25, 62.5, 125, 250 mg/kg, 납 농도는 200, 500, 1000, 2000 mg/kg 및 카드뮴의 농도는 각각 15, 30, 60, 100 mg/kg로 처리하여 실험하였다. 비소, 납 및 카드뮴 처리구에서 참김의털의 엽수는 납 처리구 (200, 500, 1000 mg/kg)를 제외하고 대조구보다 모두 감소하였고, 지상부의 길이 성장은 비소 처리구 모두에서 대조구보다 증가하였으나 지하부는 모두 감소하였고, 1000 및 2000 mg/kg 납 처리구에서는 대조구보다 증가하였으나 나머지 처리구에서는 대조구보다 모두 감소하였고, 카드뮴 처리구의 경우, 지상부는 대조구보다 모두 증가하였고, 지하부는 모두 감소하였다. 비소 처리구의 경우, 생체량은 모든 부위와 모든 농도에서 대조구보다 감소하였고, 200, 500, 1000 mg/kg 납 처리구는 지상부와 지하부 모두에서 대조구보다 생체량이 증가하였고, 카드뮴 처리구에서는 지상부와 지하부의 생체량이 대조군보다 모두 감소하였다. 중금속의 처리구 농도가 높아질수록 엽수와 식물부위별 생체량은 모두 감소하는 경향을 보였고, 중금속의 처리구 농도가 높아질수록 지상부의 길이 성장은 소폭 증가하는 경향이 있으나 지하부는 다소 감소하는 경향이 있었다. 62.5 mg/kg 비소 처리구의 지상부, 지하부의 비소축적농도는 9.4 mg/kg와 253.3 mg/kg로 지하부가 26.9배로 축적율이 높았으며 250 mg/kg 비소 처리구의 지상부는 고사한 반면 지하부의 비소축적농도는 859.1 mg/kg로 분석되었고, 2,000 mg/kg 납 처리구에서 지상부와 지하부는 10,308.1 및 11,012.0 mg/kg로 지상부가 지하부의 1.1배 높게 축적되었고, 100 mg/kg 카드뮴 처리구에서 지상부와 지하부는 176.0 및 287.2 mg/kg로 지하부가 지상부의 1.6배 높게 축적되었다. 참김의털의 내성평가 결과, 비소, 납, 카드뮴의 모든 처리구에서 고사하지 않고 생장을 유지하여 3종의 중금속에 다재내성이 확인되었다. 참김의털의 납 오염토양에 대한 식물추출 (phytoextraction)은 납 오염농도 2,000 mg/kg까지 적용할 수 있는 식물종으로 검증되었다.

• 한국식물생명공학회:학술대회논문집
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• 한국식물생명공학회 2003년도 식물바이오벤처 페스티발
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• pp.139-139
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• 2003

• Plant Resources
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• 제7권3호
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• pp.200-205
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• 2004

Sweet potato infected with a viral disease (SPFMV) showed irregular chlorotic patterns, so called feathering associated with faint or distinct ring spots that have purple-pigmented borders. SPFMV was eliminated from sweet potato plants using meristem tip culture. MS medium supplemented with BAP (2mg/L) and NAA (0.05 mg/L) was used for shoot proliferation and 1/2 MS medium for rooting of the plants. Highest percentage of regenerated plants (60%) was obtained from the optimum size (0.3-0.5mm) meristem tips. Of these, 60% plants were found negative for SPFMV by RT-PCR. Virus detection by RT-PCR was found to be a reliable method. Meristem-tip culture to produce SPFMV-free quality sweet potato and virus detection by RT-PCR is an efficient, time saving and reliable method for production of SPFMV-free tissue culture raised plants.

• Journal of Forest and Environmental Science
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• 제35권1호
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• pp.41-46
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• 2019

In vitro organogenesis system of the valuable ornamental species, Prunus yedoensis which is native to Korea, was established through callus culture derived from root tissues. Callus were induced on the medium supplemented with 2,4-D and BA or NAA and kinetin. Organogenesis was differ from the callus type, and NAA and kinetin combination was effective to induce organogenic callus. Growth of callus was influenced by sucrose concentrations. High level of sucrose (over 5%) had adverse effects such as decreased fresh weight and increased mortality of callus. Shoots developed from the callus when $GA_3$ was treated with BA in the medium. Results showed that $GA_3$ is essential for shoot development and elongation from callus in this species.

• 한국전기전자재료학회:학술대회논문집
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• 한국전기전자재료학회 2009년도 하계학술대회 논문집
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• pp.11-12
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• 2009

In this paper, we propose a high voltage driver IC(HVIC) for LCD and PDP TV power supply. The proposed circuit is included novel a shoot-through protection and a pulse generation circuit for the high voltage driver IC. The proposed circuit has lower variation of dead time and pulse-width about a variation of a process and a supply voltage than a conventional circuit. Especially, the proposed circuit has more excellent pulse-width matching of set and reset signals than the conventional circuit. Also the proposed pulse generation circuit prevent from fault operations using a logic gate. Dead time and pulse-width of the proposed circuit are typical 250 ns, and its variation is maximum 170 ns(68 %) about a variation of a process and a supply voltage. The proposed circuit is designed using $1\;{\mu}m$ 650 V BCD process parameter, and a simulation is carried out using Spectre.

• Journal of Power Electronics
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• 제11권2호
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• pp.120-131
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• 2011

This paper proposes two control strategies for the bidirectional Z-source inverters (BZSI) supplied by batteries for electric vehicle applications. The first control strategy utilizes the indirect field-oriented control (IFOC) method to control the induction motor speed. The proposed speed control strategy is able to control the motor speed from zero to the rated speed with the rated load torque in both motoring and regenerative braking modes. The IFOC is based on PWM voltage modulation with voltage decoupling compensation to insert the shoot-through state into the switching signals using the simple boost shoot-through control method. The parameters of the four PI controllers in the IFOC technique are designed based on the required dynamic specifications. The second control strategy uses a proportional plus resonance (PR) controller in the synchronous reference frame to control the AC current for connecting the BZSI to the grid during the battery charging/discharging mode. In both control strategies, a dual loop controller is proposed to control the capacitor voltage of the BZSI. This controller is designed based on a small signal model of the BZSI using a bode diagram. MATLAB simulations and experimental results verify the validity of the proposed control strategies during motoring, regenerative braking and grid connection operations.

• 한국정보통신학회논문지
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• 제10권9호
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• pp.1617-1624
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• 2006

본 논문은 분산전원시스템(DGS)을 위 한 시스템 모델링, 수정된 SVPWM 적용 그리고 수동소자인 L과 C로 구성된 Z-원 인버터의 제어기에 대하여 기술하였다. 기존의 DC/DC 승압 컨버터나 변압기를 사용하지 않고 낮은 DC 입력을 상용 AC로 만들기 위해서 SVPWM의 영벡터 구간을 이용해 DC-링크단 전압을 승압하는데 이용하였다. 한 스위칭주기에 3개조의 스위칭소자 중에 2개조만 동시 도통되도록 하여 유효벡터의 손실 없이 승압이 가능하도록 하였다. 빠르고 오버슈트가 없는 전류응답과 낮은 정상상태 전압오차를 얻기 위해서 이산시간 슬라이딩모드 전류제어기와 강인한 서보기구 전압제어기를 설계하였다. 시뮬레이션을 통하여 제안된 알고리즘의 유용성을 확인하였다.