• 한국산림과학회지
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• 제86권4호
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• pp.430-434
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• 1997

희귀 수종인 산개나리(3년생)의 산초지 액아를 배양하여 식물체를 유도하였다. 효과적인 증식법 구명을 위해 MS 배지에서 세 가지 싸이토키닌(BAP, kinetin, zeatin)의 농도별 효과를 조사하였다. 증식은 식물 생장조절 물질이나 농도별 효과가 뚜렷하지 않았으나 zeatin 처리시 줄기 및 잎의 발달에 효과가 있었다. kinetin 처리는 줄기 생장과 더불어 모두 발근되는 특징을 보였고, BAP와 zeatin은 발근은 가능하나 고농도로 갈수록 발근율이 감소하였다. 황화처리는 줄기의 생장을 촉진하였다. 줄기는 3년 이상의 계대배양 후에도 정상적인 증식이 가능했다. 발근된 식물체는 인공 배양토에서 100% 활착되었으며 산지에 이식하여 정상 생장하였다. 이상의 결과는 희귀 수종인 산개나리의 기내 번식으로 자생지의 복원이 가능함을 시사한다.

• Journal of Plant Biotechnology
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• 제31권2호
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• pp.127-132
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• 2004

본 실험은 산마늘의 산초 증식과 인경 형성에 적합한 생물반응기 배양방식과 인경 형성시 배양방식에 따른 당 대사를 구명하고자 실시되었다. 다신초 증식에는 생물반응기에 망을 걸고 배지에 절편체를 접하게 배양한 RC와 MRC (13-15개)에서 가장 좋은 결과를 얻었다. 인경형성과 비대에는 간헐적으로 배지를 공급해준 E&FS에서 93.4％의 인경형성이 이루어졌고 크기에 있어서도 균일하였다. RC와 MRC에서 형성된 인경은 뿌리가 무성하였으며 인경의 크기도 균일하지 않았다. 배양방식별로 수확한 인경내 유리당 함량은 전반적으로 E&FS.에서 낮았던데 반해 전분 함량은 높았다. 배지내 sucrose, glucose와 fructose는 인경 비대 시기에 감사되었는데 이는 첨가한 sucrose가 가수분해 되기도 전에 glucose나 fructose와 함께 식물체로 바로 이용됨을 보여주는 것이었다.

• 한국작물학회지
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• 제62권3호
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• pp.259-274
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• 2017

Platycodon grandiflorum (Bell flower) is an important plant that has traditionally been used as herbal medicine for the treatment of cough, phlegm, sore throats, lung abscesses, chest pains, dysuria, and dysentery. The present study was initiated to investigate the feasibility of inducing shoot and root organogenesis in cultured explants of P. grandiflorum in a range of culture media and through use of various plant growth regulators (PGRs). The plantlets (Stem containing one node) were isolated and cultured on different concentrations of Murashige and Skoog (MS) medium supplemented with PGRs. We found that proliferation and elongation of shoots and roots could be achieved on 1/4 MS for P. grandiflorum with wild and green petals and on 1/8 MS for P. grandiflorum with double petals. The highest levels of development and elongation of adventitious shoots and roots were observed when petal explants were cultured on 1/4 MS (pH 3.8) supplemented with 5% sucrose. Increasing the agar concentration reduced shoot growth and rooting potential; nevertheless, the highest number of shoots and roots was observed on 0.6% agar. In the case of growth regulators, 1/4 MS supplemented with $1mg\;L^{-1}$ 6-benzylaminopurine (BA) was found to be best for shooting, although higher concentrations of BA tended to reduce shoot and root elongation. The highest number of shoots was achieved on $0.5mg{\cdot}L^{-1}$ thidiazuron (TDZ) from double petal explants grown on 1/8 MS. However, root and shoot elongation were found to decrease when TDZ concentrations were increased. Low concentrations of kinetin, naphthalene acetic acid, indole acetic acid, and 3-indole butyric acid induced shoot and root proliferation and elongation. Taken together, our study showed that low concentrations of PGRs induced the greatest root formation and elongation, showing that the optimal concentration of PGRs for shoot proliferation was species-dependent.

• Journal of Plant Biotechnology
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• 제7권4호
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• pp.247-257
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• 2005

An efficient, rapid and large-scale in vitro clonal propagation of agronomically important Indian cereal crop genotypes (NSH27 & K5) of Sorghum bicolor (L.) Moench. by enhanced shoot proliferation in shoot tip segments was designed. MS medium fortified with plant growth regulators and coconut water markedly influenced in vitro propagation of Sorghum bicolor. In vitro plantlet production system has been investigated on Murashige and Skoog (MS) medium with the synergistic combination of 6-benzyladenine ($22.2\;{\mu}M$), kinetin ($4.6\;{\mu}M$), adenine sulphate ($2.8\;{\mu}M$), 5% coconut water and 3% sucrose which promoted the maximum number of shoots as well as beneficial shoot length. Subculturing of shoot tip segments on a similar medium enabled continuous production of more than 100 healthy shoots with similar frequency. When the healthy shoot clumps were cultured on MS medium fortified with 6-benzyladenine ($22.2\;{\mu}M$), kinetin ($4.6\;{\mu}M$), adenine sulphate ($2.8\;{\mu}M$), ${\alpha}$-naphthaleneacetic acid ($2.7\;{\mu}M$), ascorbic acid ($30.0\;{\mu}M$) and 5% coconut water, a rapid production of axillary and adventitious buds was developed after 8 wk culture. More than 300 shoots were produced 10 wk after culture. Rooting was highest (100%) on half strength MS medium containing 22.8 mM IAA. Micropropagated plants established in garden soil, farmyard soil and sand (2:1:1) were uniform and identical to the donor plant with respect to growth characteristics. These plants grew normally without showing any traits.

• Journal of Plant Biotechnology
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• 제32권4호
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• pp.281-285
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• 2005

우리나라 자생식물인 산호수 (Ardisia pusilla DC)를 기내에서 대량증식하기 위하여 초대배양으로 산호수 경정을 MS, 1/2MS 배지에 BA와 TDZ의 농도를 달리하여 첨가된 배지에 치상하여 배양하였다. 무기염류를 달리한 배지와 생장조절물질 농도를 다르게 한 배지 중 1/2MS 배지에 BA 0.5mL가 첨가된 배지가 신초 수 7개 이상으로 증식이 양호하여 산호수의 초대배양에 적정배지로 나타났다. TDZ를 첨가한 배지에서는 캘러스형성이 과다하여 부정아 형성을 오히려 방해하여 BA 첨가배지보다 신초증식이 양호하지 못하였다. 신초의 증식을 위해 1/2 MS 배지에 BA 0.5 mg와 sucrose의 농도를 달리하여 첨가한 결과, sucrose 농도 20 g/L를 첨가한 배지에서 신초의 증식, 생장 및 다아체 형성이 양호하였다. 형성된 신초의 발근은 1/2MS 배지에 IBA, NAA를 각각 첨가한 배지를 사용하였는데 두 가지 옥신류에서 0.5 mg의 농도에서 신초의 생장과 발근이 양호하였다. 잎과 뿌리가 잘 발달한 유식물체를 원예용 상토에 옮겨 키웠을 때 모든 개체가 정상적인 형태로 성장하였다.

• Journal of Plant Biotechnology
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• 제30권2호
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• pp.179-184
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• 2003

In order to establish micropropagation system Anthurium andreanum 'Atlanta', dwarf type, shoots of A. andreanum were cultured on medium supplemented with cytokinin. Callus was formed from the base of shoots. high frequency callus induction was obtained on medium with 10.0mg/L BA or 10.0mg/L TDZ(thidiazuron) at more than 71.8%. The shoots were cultured on media with various combinations and concentrations of TDZ, BA and 2.4-D to enhance callus induction. Callus was induced at more than 72.6% and grew vigorously on media containing 10.0mg/L BA and 0.0∼0.5mg/L 2.4-D, or 1.0mg/L TDZ. Stimulation effects of cytokinin by 2.4-D did not occur in combined treatments of cytokinin and 2.4-D. Callus was cut into sections(7${\times}$10mm), and then cultured on media with BA alone or BA and 2.4-D to regenerate shoots and to stimulate the callus growth. Shoot regeneration and callus growth were effective on media with 10.0mg/L BA alone, or 10.0mg/L BA and 0.1mg/L 2.4-D. In combined treatments of BA and 2.4-D, stmulation effects of cytocinin by 2.4-D also did not occur. Callus growth was decreased, accordiong to increasing the concentration of 2.4-D. In cimbined treatments of TDZ and 2.4-D in shoot regeneration and callus proliferation, stimulated effects of cytokinin by 2.4-D did not occur entirely. Media with 0.5∼1.0mg/L TDZ ingibited the regeneration and rooting of shoots, and callus growth from callus sections. Addition of 2.4-D on medium with TDZ ingibited the regeneration and rooting of shoots, and callus growth. Rooted plantdts were acclimatized in greenhouse. The plantlets were survived more than 98% in soil of vermiculite alone or mixed perlite 1 and vermiculite 1.

• Journal of Plant Biotechnology
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• 제34권3호
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• pp.189-195
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• 2007

Helicteres isora is medicinally important plant effective against asthma, diabetes, hypolipidemia, HIV, besides a good source of diosgenin. Seed dormancy and low rate of natural fruit production make this plant a perfect candidate for developing an in vitro method useful for its clonal propagation and further biotechnological developments. This is the first report on in vitro production of this plant. Nodal explants obtained from aseptically germinated seedlings were cultured on MS medium (Murashige and Skoog 1962) fortified with indole-3-acetic acid (IAA) ($0.57-22.83\;{\mu}M$), indole-3-butyric acid (IBA) ($0.41-16.58\;{\mu}M$), 6-benzylaminopurine (BA) ($0.44-17.75\;{\mu}M$) and kinetin (Kin) ($0.46-13.94\;{\mu}M$) either singly or in combinations of IAA + BA, IAA + Kin and BA + Kin. Combinations of cytokinins (BA and Kin) were most suitable for multiple shoot induction and $13.94\;{\mu}M\;Kin\;+\;13.31\;{\mu}M\;BA$ was optimum (79% frequency) associated with high number of microshoots (7.1 shoots per explant) after 20 days of culture. Maximum shoot elongation and proliferation (10 shoots per explant with 4.8 cm average height) was achieved on MS media containing $2.32\;{\mu}M\;Kin\;+\;2.22\;{\mu}M\;BA\;+\;2.85\;{\mu}M\;IAA$. High rooting frequency (70%) was achieved on MS medium (1/2 basal strength) fortified with $4.14\;{\mu}M$ IBA, while activated charcoal showed inhibitory effects on rooting. Hardening was done with 76% survival rate and these plants were growing without any visual defects and morphologically mimicking the naturally growing plants.

• Journal of Plant Biotechnology
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• 제30권3호
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• pp.269-273
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• 2003

In order to develop an efficient micropropagation protocal for Eucalyptus pellita, on in vitro culture system has been was established by inducing axillary buds from greenhouse stock materials. Among 6different media tested, DKW medium was the best ot induce bast induce both shoot proliferation and growth. Average number of proliferated shoots of 403per explant was obtained at the concentration of 0.1mg/LBA. Most of the stem materials excreted phenolic compounds at the proximal part of the explant and caused darking of the media. Therefore, it was necessary to transfer frequently to a fresh medium and/or to add activated charcoal at the concentration of 0.02％(w/v). Generally on vitro roots were formed easily on 1/2DKW medium with NAA treatment. All the explants rooted at the medium containing 0.2mg/L NAA and displayed vigorous root growth in vitro culture conditions. After transferred to an artificial soil mixture (peatmoss: vermiculrite: perlite, 1:1:1, v/v/v) in the greenhouse, most rooted plantlets survived well without any morphological abnormalities. The results show that the species can be micropropagated effectively by the application of axillary bud culture system.

• Journal of Plant Biotechnology
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• 제6권3호
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• pp.199-204
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• 2004

The medicinal value of the genus Cleome justifies bio-technological studies of Cleome rosea, a Brazilian annual species from sandy coastal ecosystems (restinga), which have been submitted to an intense process of antropogenic degradation. In the present work, was analyzed the influence of cytokinins, 6-benzyladenine (BA) and 6-furfurylaminopurine (kinetin) added to the Murashige and Skoog medium (MS), on the proliferation capacity of explants from the stem axis (hypocotyl, node and internode) for a period of five monthly subcultures (150 days). Regardless of the explant sources, plantlet regeneration by direct and indirect organogenesis was observed. The largest number of shoots proliferated through direct organogenesis was obtained on medium with 4.4 $\mu{M}$ BA. Also, the highest proliferation capacity through indirect organogenesis was found on medium with 4.4 $\mu{M}$ BA + 4.6 $\mu{M}$ kinetin. The presence of kinetin alone was not effective for multiplication of the species. Elongation and rooting were obtained when shoots were transferred onto growth regulator-free medium, and acclimatization rates from 70% to 81% were achieved depending on explant sources used. Plants were then successfully established in soil and showed normal phenotypes.

• 원예과학기술지
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• 제19권1호
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• pp.34-38
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• 2001

사과 M.9 자근대목의 효과적인 기내 급속 대량 번식법을 구명하기 위하여 본 연구를 수행한 결과, 신초 증식에 적합한 배지는 $1.0mg{\cdot}L^{-1}$ BA, $0.1mg{\cdot}L^{-1}$ IAA, $30g{\cdot}L^{-1}$ sucrose, $8g{\cdot}L^{-1}$ agar가 첨가된 MS 배지이며, 치상방법은 신초 정단부를 제거한 후 수평치상하는 것이 효과적이었다. 발근에 적합한 배지는 $0.5mg{\cdot}L^{-1}$ IBA, $20g{\cdot}L^{-1}$ sucrose, $8g{\cdot}L^{-1}$ agar가 첨가된 1/2 MS 배지였다.