• Korean Journal of Soil Science and Fertilizer
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• v.39 no.4
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• pp.185-194
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• 2006

A fungal strain of Trichoderma having strong chitinolytic activity was isolated from field soil enriched with crabshell for several years. Based on 5.8S rRNA, partial 18S, 28S rRNA genes, ITS1, ITS2 sequence analysis and morphological characteristics, the fungus was identified as Trichoderma asperellum and named as Trichoderma asperellum T-5 (TaT-5). The fungus released lytic enzymes such as chitinase and ${\beta}$-1, 3-glucanse, and produced six antifungal substances in chitin broth medium. To demonstrate the protective effect of TaT-5 against damping-off in cucumber plant caused by Rhizoctonia solani, TaT-5 culture broth (TA), chitin medium (CM) and distilled water (DW) were applied to each pot at 10 days after sowing, respectively. Then, the homogenized hyphae of R. solani were infected to each pot at 1 week after TaT-5 inoculation. During experimental period, fresh weight of shoot and root in cucumber plant more increased at TA treatment compared to other treatments. PR-proteins (${\beta}$-1, 3-glucanase and chitinase) activities in cucumber leaves markedly increased at CM and DW treatments, but the activity slightly increased and then decreased at TA treatment at 3 days after infection of R. solani. The activity of PR-proteins activities in cucumber roots at all treatments decreased with time where the degree of decrement was more alleviated at TA treatment than CM and DW. These results suggest that the lytic enzymes (chitinase and ${\beta}$-1, 3-glucanse) and antifungal substances produced by TaT-5 can reduce the pathogenic attack by R. solani in cucumber plants.

• Journal of Bio-Environment Control
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• v.23 no.4
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• pp.383-390
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• 2014

This study was conducted to investigate the growth characteristics of cucumber scion and pumpkin rootstock under different levels of light intensity (photosynthetic photon flux, PPF) and plug cell size in a closed transplant production system with artificial lighting. Cucumber scion and pumpkin rootstock seedlings were grown under the combinations of three levels of PPF (PPF 165, 248, and $313{\mu}mol{\cdot}m^{-2}{\cdot}s^{-1}$) and five types of plug tray (50, 72, 105, 128, and 200 cells in the tray) for nine days. The shoot dry weight and relative growth rate increased with increasing PPF and plug cell size. As PPF increased, cucumber scion and pumpkin rootstock seedlings had higher dry matter, lower specific leaf area, and lower hypocotyl length. The first true leaf of cucumber scion and pumpkin rootstock unfolded at eight and seven days after sowing, respectively, except the treatment using 200-cell plug tray. The unfolding of first true leaf of seedlings grown in 200-cell plug tray was delayed by one day. Accordingly, it was considered that the use of small cell size such as 200-cell plug tray would require more time for the production of scion and rootstock. Based on the results, we suggest that cucumber scion and pumpkin rootstock be grown in 105-cell to 128-cell plug tray for eight days and 72-cell to 105-cell plug tray for seven days, respectively, when using splice grafting method with root-removed rootstock. Additionally, higher PPF is suggested to improve the growth and quality of scion and rootstock.

• Journal of Korean Society of Forest Science
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• v.76 no.2
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• pp.99-108
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• 1987

Half-sib seeds and one-year-old seedlings of Ginkgo biloba were treated with various simulated acid rains (pH 2.0, pH 3.0, pH 4.0 and pH 5.0) to examine the effects of acid rain on seed germination and seedling growth. The seeds were sown in a pot ($4500cm^3$) containing one of three different soils (nursery soil, mixed soil and sandy soil) and the seedlings were grown in the same pots as the seeds. Simulated acid rain was made by diluting sulfuric and nitric acid solution ($H_2SO_4$: $HNO_3$ = 3:1, V/V) with tap water and tap water (pH6.4), and treated by 5mm each time for three minutes during the growing seasons (April to October 1985 and April to August 1986). Acid rain treatments were done three times per week to potted seeds and seedlings by spraying the solutions. The seed germination, seedling growth and physiological characteristics of potted seedlings were compared among three soil types as well as among the various pH levels. The results obtained in this study were as follows: 1. Seed germination of Ginkgo biloba decreased significantly at pH 2.0 level in the field test, and also at the levels of both pH 2.0 and pH 3.0 in the laboratory test, compared to that at control. 2. For two-year-old seedlings, total, top and root dry weights per seedling were significantly different among the three soil types and among the levels of pH, and shoot growth was different only among the levels of pH. 3. For one-year-old seedlings, height and total and stem-branch dry weights per seedling were significantly different among the levels of pH.

• Journal of Bio-Environment Control
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• v.24 no.3
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• pp.178-186
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• 2015

This study was carried out to determine the effects of environment controls (temperature and shading level) on germination responses and early growth of Allium thunbergii. Germination experiment was performed by pre-treatment (with low temperature and wetting treatments for 0, 20, 40 and 60 days) and temperature controls (5, 10, 15, 20, 25 and 30). And growth experiment was performed by containers (128 and 200 cavities containers) and shading level (full sunlight (control), 35%, 50% and 75% shading). Germination rate of A. thunbergii seeds were, 20 days of seed pre-treatment, the highest at $10^{\circ}C$ (81.7%) and the more temperature went up, the more germination rate went down. As a result of surveying container and shading treatments, the height, leaf area, leaf length, leaf aspect ratio (L/W) were higher under 50% shading of 128 (24.2cm, $2.76cm^2$, 22.3cm and 223.4, respectively) and 200 (22.6cm, $2.29cm^2$, 19.4cm and 190.5, respectively) cavities container. The root was grown well under full sunlight. Specially, fresh weight of shoot (leaves+stem) was higher under 50% shading of 128 (0.241g) and 200 (0.212g) cavities container. As a result of surveying the whole experiment, A. thunbergii seeds need to pre-treatment (with low temperature and wetting treatments for 20~40 days) for high germination rate. And it is judged better growth and higher yield by maintaining 50% shading of 200 cavities container.

• Food Science and Preservation
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• v.23 no.5
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• pp.623-630
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• 2016

Inappropriate storage of fresh-cut onions may result in losses of good quality. To understand storage conditions for shelf-life and quality of fresh-cut onions, The effect of packing type and storage temperature on the quality of fresh-cut onions was evaluated. Onions stored at $0^{\circ}C$ for 2 months were peeled off after removing root and shoot parts. Each three peeled onions were packed in a polyethylene film (PE, $50{\mu}m$) or in a polyethylene/polypropylene film (PE/PP, $100{\mu}m$) with vacuum treatment (70 cmHg) and stored at different temperatures (4, and $10^{\circ}C$) for 21 days. The following analyses were examined to evaluate the quality of fresh-cut onions: microbial population, surface color, titratable acidity and pH, respiration rate, and sensory quality. Fresh-cut onions stored at $4^{\circ}C$ showed less aerobic and coliform bacterial population than those stored at $10^{\circ}C$ during observation periods. Fungal populations of fresh-cut onions packed in PE film stored at $10^{\circ}C$ increased significantly after 13 days. E. coli was not detected in all treatments during whole storage periods. Surface colors of fresh-cut onions were not affected by packing type and storage temperature, however, color difference (${\Delta}E$) of fresh-cut onions in PE/PP film stored at $10^{\circ}C$ was significantly higher than those of other treatments. Titratable acidity of fresh-cut onions was not affected by packing type and storage temperature. However, pH of fresh-cut onions packed in PE film stored at $10^{\circ}C$ increased gradually over the whole storage period. Fresh-cut onions packed in PE film showed higher $CO_2$ and less $O_2$ concentrations at $10^{\circ}C$ than those at $4^{\circ}C$. The sensory quality of fresh-cut onions was significantly affected by packing type and storage temperature after 13 days. Particularly, vacuum treatment in PE/PP film showed better sensory quality than that of PE film package at the same storage temperature. It was concluded that vacuum treatment and storage at $4^{\circ}C$ could be effective to prolong the quality of fresh-cut onions up to 21 days.

• Journal of Bio-Environment Control
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• v.31 no.1
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• pp.14-21
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• 2022

Uniform scions and rootstocks should be produced to ensure grafting success. Light quality is an important environmental factor that regulates seedling growth. The effects of warm- and cool-white light emitting diode (LED) ratios on seedling growth were investigated. Scions and rootstocks of cucumber, tomato, and watermelon were grown in a closed transplant production system using LED as the sole lighting source. The LED treatments were W1C0 (only warm-white), W1C1 (warm-white: cool-white = 1:1), W3C1 (warm-white: cool-white = 3:1), and W5C2 (warm-white: cool-white = 5:2). The seedlings grown in W1C1 had the shortest hypocotyls, and the seedlings grown in W1C0 had the longest hypocotyls among the three tested vegetables. The hypocotyls of watermelon scions, watermelon rootstocks, and tomato rootstocks were shortest in W1C1, followed by those in W3C1, W5C2, and W1C0, but there was no significant difference between W3C1 and W5C2, which remained the same as the ratio of cool-white LEDs increased. In addition, tomato scions had the first and second longest hypocotyls in W1C0 and W3C1, respectively, and the shortest hypocotyls in W5C2 and W1C1, along with W5C2 and W1C1, although the difference was not significant. The stem diameter was highest in W1C0 except for tomato seedlings and rootstocks of watermelon. The shoot fresh weight of scions and rootstocks of cucumber and watermelon and the root fresh weight of cucumber scions were lowest in W1C1. These results indicated that different ratios of LED lighting sources had a strong effect on the hypocotyl elongation of seedlings.

• Korean Journal of Environmental Agriculture
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• v.16 no.2
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• pp.130-135
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• 1997

Tomatoes are flooded differently 0, 5, 10 and 15cm, according to the developing stages such as flowering stage under the condition of greenhouse. Along with this, they are treated according to the time condition such as 6, 12, 24, 48 and 120 hours. The results obtained are summarized as follows. Plant height decreased in the depth of $0{\sim}10cm$ for over 48 hours, in the depth of 15cm for over 24 hours. Number of leaves was the same as in control, and it decreased over. Number of flowers and fruit setting of individuals decreased conspicuously according as the depth and the hours got greater and longer. Adventitious root occurred remarkably in the depth of $0{\sim}10cm$, for over 24 hours and in the depth of 15cm, 12 hours. Epinastic curvature increased greatly as the depth and the hours got greater and longer. Diffusion resistance of stomata cell increased as the depth and the hours got greater and longer. Diseases occurred conspicuously as the hours of flooding got longer rather than as the depth greater. The preventing of diseases caused by insecticide was observed, but it was not greater than in the seedling and transplanting stage. Fertilization was effective in the case of increasing the weight of shoot. Number of fruits per plant did not decrease in the depth of 0cm up to 24 hours, but decreased on the deeper level of flooding and increased as the hours got longer. Moreover with the exception of 120 hours per respective depth of the treatment, average weight of a fruit got greater as the depth and the hours got greater and longer. In the case of epinastic curvature and diffusion resistance, there was negative correlation between all the other investigated characters and positive correlation between weight of a fruits and average weight of a fruit.

• Journal of Bio-Environment Control
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• v.26 no.3
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• pp.227-234
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• 2017

This study aimed to investigate the nutrient solution developed by based on nutrient-water absorption rate of strawberry 'Maehyang' by comparing growth and yield for 8 months with 5 kinds of nutrient solution with different ion composition. Strawberry plants were planted at elevated bed and supplied with five kinds of nutrient solutions (RDA), Yamazaki, PBG, University of Seoul (UOS) and NewUOS from one month onwards. Five types of nutrient solution were supplied to the strawberry plants associated with EC $1.0dS{\cdot}m^{-1}$, pH 6.0, $150{\sim}300mL{\cdot}plant^{-1}$ per day. At 60 days after planting, leaf width and leaf petiole of the strawberry plants showed significant differences among nutrient solution types and photosynthesis was higher in RDA and NewUOS nutrient solution and lower in PBG nutrient solution. The EC of the drainage on vegetative growth stage was $0.7{\sim}0.8dS{\cdot}m^{-1}$, which is lower than the supplied EC level, and to $1.0-1.2dS{\cdot}m^{-1}$, afterwards. The pH of the drainage was higher in Yamzaki solution as 6.2~6.8, while the pH of the UOS nutrient solution was lower in 5.1~5.2. Nitrate content was most absorbed in vegetative growth stage and after flower clusters development. The potassium uptake was highest at the NewUOS followed by UOS and Yamazaki nutrient solution. At six months after -planting fresh weight and dry weight of shoot and root were higher in UOS and NewUOS nutrient solution than other nutrient solutions, and the dry matter ratio was lower at 43.5% in Yamazaki nutrient solution and 30.6% in NewUOS nutrient solution than other solutions. Length, width, weight, and sugar content of the strawberries harvested from December to February were unaffected by treatment, but yield was higher in NewUOS nutrient solution due to increasing fruit number and average weight. From March to May, number of fruit was higher in Yamazaki nutrient solution. In conclusion, there was no difference in the growth of 'Maehyang' when 5 nutrient solutions were grown under hydroponics. But in order to improve the marketability, the NewUOS nutrient solution is appropriate to use from planting to February and it is suitable to use Yamazaki nutrient solution after March when temperature is high and the amount of fruit set per inflorescence.

• Korean Journal of Environmental Agriculture
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• v.25 no.1
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• pp.47-57
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• 2006

This study was carried out to select the Eco-tree for successful phytoremediation of abandoned metalliferous mines. We examined vegetation and heavy metal concentrations of woody plants in abandoned mining areas, and also conducted seed germination and seedling growth experiment on contaminated soils from Gahak and Geumjeong mines. Pinus densiflora, Robinia pseudoacacia, Lespedeza bicolor and Alnus japonica showed high frequency in the survey areas and had high heavy metal concentrations compared to other species. Heavy metal concentrations were higher in roots than in leaves and stems. The seed germination rate was in the order of P. densiflora, L. bicolor, R. pseudoacacia, and Alnus japonica from the incubactor and greenhouse experiment. In the incubator experiment germination rate was highest in the control soil for P. densiflora and A. japonica. Germination rate of P. densiflora was highest on the 100% contaminated soil for Gahak mine while germination rate decreased with increased percentage of contaminated soil for Geumjeong mine. In the greenhouse experiment germination rate was lowest on the 40% contaminated soil for Gahak mine while germination rate was lowest on the 20% contaminated soil for Geumjeong mine and increased with increased percentage of contaminated soil. Shoot growth was highest for L. bicolor while root growth was highest for R. pseudoacacia except for 20% contaminated soil in Geumjeong mine.

• Asian Journal of Turfgrass Science
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• v.23 no.2
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• pp.331-344
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• 2009

Buffalograss is an important turfgrass species with excellent cold, heat, and drought tolerance. Understanding the physiological integration of buffalograss under heterogeneous conditions helps to develop cultural practices that better use limited resources for uniform turf quality. The objective of this study was to evaluate physiological integration of buffalograss under water deficit stress and the involvement of lipid peroxidation and antioxidants in the process. In one experiment, buffalograss was planted in the center of a four-compartment growth unit. Watering frequencies, once a week(+) and once in two weeks(-), were combined with the sand(S) or peat(P) in each unit to generate five total treatments(P+S-P-S+, P+P+P+P+, S-S-S-S-, P-P-P-P-, and S+S+S+S+). The average number of shoot established from the heterogeneous root-zone medium was higher than the average of four possible homogeneous media. In second experiment, single ramet in Hoagland solution($S_0$) or single ramet in Hoagland solution with 20% PEG-6000($S_s$) were compared with two connectedramets under different treatments. Treatments for connected ramets were young ramet in Hoagland solution($Y_{os}$) and old ramet in Hoagland solution with 20% PEG-6000($O_{os}$), and old ramet in Hoagland solution($O_{ys}$) and young ramet in Hoagland solution with 20% PEG-6000($Y_{ys}$). Lipid peroxidation, antioxidants, and proline showedphysiological integration between ramets subjected to different levels of water stress. Superoxide dismutase(SOD), Guaiacol peroxidase(G-POD), malondialdehyde(MDA), and free proline also showed different time courses and relative activities during the physiological integration.