• Title/Summary/Keyword: Shewanella sp. L93

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Optimal Condition for Eicosapentaenoic Acid Production and Purification from Psychrophillic Marine Baterium Shewanella sp. L93 (호냉성 해양세균 Shewanella sp. L93로부터 Eicosapentaenoic Acid 생산 및 정제를 위한 최적화 조건)

  • Mo, Sang-Joon;Hong, Hye-Won;Bang, Ji-Heon;Cho, Ki-Woong
    • Microbiology and Biotechnology Letters
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    • v.39 no.3
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    • pp.218-223
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    • 2011
  • To obtain eicosapentaenoic acid (EPA)-producing bacteria, some 600 strains of bacteria were isolated from Antarctic sediment and marine organisms during the summer expedition of 1999-2000 and 7 EPA-producing bacteria were obtained through screening with TLC and GC. A strain designated as L93 showed the highest EPA production, which was gram-negative, rod-shaped bacterium. L93 strain was identified as Shewanella sp., from the sequence analysis of 16S rDNA. Optimal conditions temperature and pH for the growth and EPA production were about $4^{\circ}C$ and pH 7. In addition, its production was optimized by 50%(w/v) sea salt. We establish the optimal production system to produce about 320 mg per liter by using this optimal EPA production conditions. EPA-methyl ester was purified from cultured L93 strain to a purity of higher than 97% and typical purification yield is greater than 72% of the input amount via urea complexation and HPLC.

Purification and Characterization of Extracellular Protease form Psychrotrophic Antarctic Bacteria (남극에서 분리한 저온성 세균 유래 단백질 분해 효소)

  • 조기웅;방지헌;홍혜원;박승일;이윤호
    • Korean Journal of Microbiology
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    • v.38 no.4
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    • pp.254-259
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    • 2002
  • A psychrotrophic bacterium was isolated from Antarctic marine sediment and identified as Shewanella sp. species based on the biochemical properties and 16S rRNA sequence, and designated as Shewanella sp. L93. Extracellular protease produced by this strain was purified through ammonium sulfate precipitation, High-Q column chromatography, first gel permeation chromatography, BioScale Q2 ion exchange chromatography and second gel permeation chromatography, and basic properties of this enzyme were investigated.