• 한국수산과학회지
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• 제32권2호
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• pp.232-237
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• 1999

Tachykinin peptide의 구조와 생리활성간의 상관관계를 조사하기 위해 고상법으로 합성한 어류 유래의 neuropeptide $\gamma$(Mammalian-, Bowfin- 그리고 Shark-neuropeptide $\gamma$)를 사용하였다. 이들의 이차 구조를 알아보기 위해 circular dichroism (CD)을 이용하였다. CD 연구 결과에 따르면, mammalian-neuropeptide $\gamma$, bowfin-neuropeptide $\gamma$와 shark-neuropeptide $\gamma$는 완충액과 인공지질막 조건하에서 random구조를 나타내었다. 또한 이들 neuropeptide $\gamma$의 장관에 대한 수축 활성을 조사하기 위해 guinea-pig의 회장, rat의 십이지장과 carp intestine을 사용하였다. Carp intestine에 서 bowfin-neuropeptide $\gamma$>shark- neuropeptide $\gamma$>mammalian-neuropeptide $\gamma$순으로 활성 이 나타났다. 그러나, guinea-pig 회장과 rat 십이지장에 대해서 mammalian-NP$\gamma$의 활성이 어류 유래의 neuropeptide $\gamma$들보다 높게 나타났다. 이러한 결과들은 neuropeptide $\gamma$가 종-특이적인 활성을 나타냄을 제시한다.

• Fisheries and Aquatic Sciences
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• 제5권2호
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• pp.136-140
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• 2002

Every year fish skin, bone and intestines are discarded as processing waste material. The use of fish processing waste material is more economical and environmental-friendly. The leather-like material was produced using shark intestine. Physical charactistics such as tensile strength, elongation, tongue tearing strength, and bursting strength of the leather-like material were measured, and compared with those of a commercial leather product. The values of tensile strength, elongation, tongue tearing strength, and bursting strength of the leather-like material were $3.3kg/mm^2$, $53\%$, 13.0kg/mm and $18kg/cm^2$, respectively. Elongation $(l09\%)$ of the leather-like material coated with lacquer was higher than that of a commercial leather material, and the other factors were similar. The tensile strength and tongue tearing strength of the leather-like material was higher than those of shoes leather, but bursting strength was lower. These results suggested a potential value to use the leather-like material from shark intestines as a substitute for commercial leathers.

• 대한수의학회지
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• 제35권3호
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• pp.417-425
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• 1995

두툽상어의 위장관 내분비세포의 부위별 분포, 출현빈도 및 세포의 종류를 밝히고자 면역조직화학적 방법으로 관찰하였던 바, 5종의 면역반응세포가 동정되었다. 5-HT 면역반응세포는 최고의 빈도로, somatostatin 면역반응세포는 소수의 위부위와 극소수의 장부위에 걸쳐 각각 전장관에서 관찰되었다. Glucagon과 BPP 면역반응세포는 십이지장과 직장을 제외하고 다양한 빈도로 소화관 전체에서 출현하였다. 한편 Gas/CCK 면역반응세포는 소장부위에서 국한하여 소수 분포하였다. 이상에서 두툽상어의 위장관 내분비세포는 부위별 분포에 있어서 다른 종과 유사하였으나, 출현빈도는 다소 낮게 나타났음을 알 수 있었다.

• 한국식품위생안전성학회지
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• 제36권4호
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• pp.289-297
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• 2021

본 연구에서 multiplex PCR과 real-time PCR을 이용하여 창난젓의 원료를 감별할 수 있는 새로운 판별법을 개발하였다. 명태와 가이양의 종 특이 프라이머를 디자인하고, 명태와 가이양의 genomic DNA를 template로 single PCR과 multiplex PCR을 실시하였다. PCR을 실시한 결과, single PCR에서 명태(297 bp)와 가이양(132 bp)에 해당하는 PCR 밴드를 확인하였으며 교차 반응이 일어나지 않는 것을 확인하였다. Multiplex PCR에서 명태와 가이양 사이에 교차반응 없이 증폭이 일어나는 것을 확인하였다. Real-time PCR 결과, 명태 종 판별 프라이머에서 명태의 Ct 평균값은 20.765±0.691, 가이양 시료에서 Ct 평균값은 35.719±1.828이었으며, 가이양 종 판별 프라이머에서 명태 시료의 Ct 평균값은 35.996±1.423, 가이양 시료의 Ct 평균값은 20.096±0.793으로 프라이머의 효율성, 특이성 및 교차 반응성에서 유의한 차이가 나타났다. 이러한 결과를 바탕으로 시중에서 판매되는 7개 제품을 multiplex PCR 및 real-time PCR로 확인하였으며, 모든 시료에서 유효한 결과를 확인하였다. 본 연구에서 제작된 명태와 가이양에 대한 종 특이적 프라이머는 가공된 젓갈 시료의 원료의 판별 가능하며, 이러한 결과는 식품안전관리에 기여할 수 있을 것으로 기대된다.

• 동아시아식생활학회지
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• 제1권3호
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• pp.259-276
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• 1991

The review of side dishes, oereal dishes and seasonings of the banquet on the 24th year of King Kojong in Chosun Dynasty were summarized as follows: 1. Ten kinds of soups(=Tang), that is Yyolguja Tang$.$Guja Tang$.$Keumjung Tang$.$Whanja Tang$.$Jap Tang$.$Gol Tang$.$Seunggiwa Tang$.$Jeopo Tang$.$Haesam Tang$.$Yang Tang were served. The most frequently served soup among them was Yyolguja Tang. 2. Various kinds of dried meat/fish were piled up most highly among dishes. Sliced beef and minced beef among dried meats, white cud$.$yellow cud$.$flatfish$.$skate$.$shark$.$octopus$.$dried abalone$.$cuttle fish$.$dried scallop were piled up together. 3. Sliced of boiled beef/pork and gelatined knuckles were assorted. Boiled meat which was made of beef, pork, lamb, liver, stomach, brisket and plat flank of caw was chiefly used for boiled beef, hams for boiled pork, mediumsized lamb for boiled lamb. And the legs and stomach of caw, old chicken, mullet were used for gelatined knuckle. 4. Three kinds of pan-frying dishes that was stomach and liver of caw, and mullet were usually piled up, If one kind in one dish, sea cucumber and mullet were used respectively. 5. Boiled abalone was dried abalone with soaked, sliced and boiled down in soysauce. 6. Roasted dish was usually made of assorted food stuffs. Sometimes chicken an pheasant was used for roasted dish. 7. Steamed dish was like this : assorted beef, fresh abalone, sea cucumber, mullet, young pork, chicken, dried abalone. 8. Raw dish was made of intestine of caw, fresh abalone and mullet. 9. Poached egg was serving decorated with red pepper, pine-nut and green onion. 10. Seasoned green-been-jelly was served only one time. 11. Radish kimchi was served only one time. 12. Chief foods were nuddle and stuffed bun. 13. Served seasings were honey, mustard, soysauce with vinegar, salt. Review from banquet menu, 34 kinds of side dishes were served : 10 kinds of soup, dried fish/meat, boiled beef, boiled pork, gelatined knuckle, 5 kinds of panfrying, boiled-down-abalone in soysauce, 3 kinds of roast, 5 kinds of steamed, 3 kinds of raw dishes, pouched egg, green-bean-jelly, kimchi. 4 kinds of chief foods were served : nuddle, dry nuddle, 2 kinds of dumping.

• 한국수산과학회지
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• 제37권6호
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• pp.445-454
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• 2004

A search for contraction and relaxation responses on the smooth muscles, antimicrobial and antioxidant activity in different body parts of 4 freshwater fish and 4 marine fish were conducted. The four freshwater fish studied were Sarotherodon niloticus (tilapia), Channa arus (snake head), Cyprinus carpio (Israel carp) and Siluru osotus (catfish), and the four marine fish were Scyliorhinus torazame (tiger shark), Ateiepus japonicus (tadpole fish), Mugil cephalus (gray mullet) and Thamnaconus modestus (file fish). Frozen samples were extracted with distilled water containing $1{\%}$ acetic acid. Antimicrobial activity against Bacillus subtilis and Eschrrichia coli was detected in extracts from several tissues in all species tested. Relatively high antimicrobial activity could also be detected in the bile extracts from C. carpio, M. cephalus, and T. modestus. Contraction and relaxation responses on smooth muscles could be detected in all species tested, especially in the intestine extracts. Antioxidant activity was also detected in extracts from several tissues in all species tested, while in the extracts from S. osotus, excluding livers and spleens, no antioxidant activity was detected. Results from this study suggest that fish are a potential source for the discovery of novel bioactive materials.