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Biologicnl Activity of the Extracts of the Eight Korean Fish Species

한국산 어류 8종 추출물의 생리활성

  • BAE Yun Jung (Department of Biotechnology and Bioengineering, College of Fisheries Science, Pukyong National University) ;
  • KIM Chan-Hee (Department of Biotechnology and Bioengineering, College of Fisheries Science, Pukyong National University) ;
  • KIM Eun Jung (Department of Biotechnology and Bioengineering, College of Fisheries Science, Pukyong National University) ;
  • GO Hye-Jin (Department of Biotechnology and Bioengineering, College of Fisheries Science, Pukyong National University) ;
  • KIM In Hae (Department of Biotechnology and Bioengineering, College of Fisheries Science, Pukyong National University) ;
  • PARK Hee Yeon (Biotechnology Researt Center, National Fisheries Research & Development Institute) ;
  • YOON Ho Dong (Biotechnology Researt Center, National Fisheries Research & Development Institute) ;
  • CHANG Young-Chae (Department of Patholgy School of Medicine, Catholic University of Daegu) ;
  • HONG Yong-Ki (Department of Biotechnology and Bioengineering, College of Fisheries Science, Pukyong National University) ;
  • PARK Nam Gyu (Department of Biotechnology and Bioengineering, College of Fisheries Science, Pukyong National University)
  • 배윤정 (부경대학교 수산과학대학 생물공학과) ;
  • 김찬희 (부경대학교 수산과학대학 생물공학과) ;
  • 김은정 (부경대학교 수산과학대학 생물공학과) ;
  • 고혜진 (부경대학교 수산과학대학 생물공학과) ;
  • 김인혜 (부경대학교 수산과학대학 생물공학과) ;
  • 박희연 (국립수산과학원 생명공학연구단) ;
  • 윤호동 (국립수산과학원 생명공학연구단) ;
  • 장영채 (대구가톨릭대학교 의과대학) ;
  • 홍용기 (부경대학교 수산과학대학 생물공학과) ;
  • 박남규 (부경대학교 수산과학대학 생물공학과)
  • Published : 2004.12.01

Abstract

A search for contraction and relaxation responses on the smooth muscles, antimicrobial and antioxidant activity in different body parts of 4 freshwater fish and 4 marine fish were conducted. The four freshwater fish studied were Sarotherodon niloticus (tilapia), Channa arus (snake head), Cyprinus carpio (Israel carp) and Siluru osotus (catfish), and the four marine fish were Scyliorhinus torazame (tiger shark), Ateiepus japonicus (tadpole fish), Mugil cephalus (gray mullet) and Thamnaconus modestus (file fish). Frozen samples were extracted with distilled water containing $1{\%}$ acetic acid. Antimicrobial activity against Bacillus subtilis and Eschrrichia coli was detected in extracts from several tissues in all species tested. Relatively high antimicrobial activity could also be detected in the bile extracts from C. carpio, M. cephalus, and T. modestus. Contraction and relaxation responses on smooth muscles could be detected in all species tested, especially in the intestine extracts. Antioxidant activity was also detected in extracts from several tissues in all species tested, while in the extracts from S. osotus, excluding livers and spleens, no antioxidant activity was detected. Results from this study suggest that fish are a potential source for the discovery of novel bioactive materials.

Keywords

References

  1. Ames, B.N., M.K. Shigenaga and T.M. Hagen. 1993. Oxidants, antioxidants, and the degenerative diseases of aging. Proc. Natl. Acad. Sci. USA, 90, 7915-7922 https://doi.org/10.1073/pnas.90.17.7915
  2. Bateman, A., R.J. MacLeod, P. Lembessis, J. Hu, F. Esch and S. Solomon. 1996. The isolation and characterization of a novel corticostatin/defensin-like peptide from the kidney. J. Biol. Chem., 271, 10654-10659 https://doi.org/10.1074/jbc.271.18.10654
  3. Birkemo, G.A., T. Luders, Q. Andersne, I.F. Nes and J. Nissen-Meyer. 2003. Hipposin, a histone-derived anti-microbial peptide in Athlantic halibut (HippogfossushippogIossus L.) Biochim. Biophys. Acta, 1646, 207-215 https://doi.org/10.1016/S1570-9639(03)00018-9
  4. Bonetto, V., M. Anderson, T. Bergman, R. Sillard, A. Norberg, V. Mutt and H. Jomvall. 1999. Spleen antibacterial peptides: high levels of PR-39 and presence of two forms of NK-lysin. Cell. Mol. Life Sci., 56, 174-178 https://doi.org/10.1007/s000180050016
  5. Chauvet, J., Y. Rouille, C. Chauveau, M.T. Chauvet and R. Acher. 1994. Special asvatocin and phasvatocin, two newoxytocin-like peptides in the spotted dogfish (Scyliorhinus caniculus). Proc. Natl. Acad. Sci. USA., 91, 11266-11270 https://doi.org/10.1073/pnas.91.23.11266
  6. Cole, A.M., P. Weis and G. Diamond. 1997. Isolation and characterization of pleurocidin, an antimicrobial peptide in the skin secretions of winter flounder. J. Biol. Chem., 272, 12006-12013
  7. Donia, M. and M.T. Hamann. 2003. Marine natural products and their potential applications as anti-infective agents. Lancet Infect Dis., 3, 338-348 https://doi.org/10.1016/S1473-3099(03)00655-8
  8. Dunlap, W.C. and Y. Yamamoto. 1995. Small-molecule antioxidants in marine organims: antioxidant activity of mycosporine-glycine. Comp. Biochem. Physiol., 112B, 105-114
  9. Elliott, G.R. and J.D. Barchas. 1979. Neuroregulators; neurotransmitters and neuromodulators. Behavior. Brain Sci., 2, 423-424 https://doi.org/10.1017/S0140525X00063445
  10. Erspamer, V. and A. Anastasi. 1962. Structure and pharmacological actions of eledoisin, the active undecapeptide of the posterior salivary glands of Eledone. , 18, 58-59 https://doi.org/10.1007/BF02138250
  11. Hadley, M.E. 1996. Gastrointestinal Hormones, Endocrinology, 4th ed., Prentice-Hall Inc., New Jersey, pp. 204-230
  12. Hofmann, A.F. 1998. Bile secretion and the enterohepatic circulation of bile acids, In: Sleisenger and Fordtran's Gastrointestinal and Liver Disease, 6th ed., Feldman, M.B.F. Scharschmidt, M.H. Sleisenger eds., W.B. Saunders, Co., Philadelphia, pp. 937-948
  13. Holmgren, S. and J. Jensen. 2001. Evolution of vertebrate neuropeptides. Brain Res. Bull., 55, 723-735 https://doi.org/10.1016/S0361-9230(01)00556-1
  14. Holye, C.H.V. 1998. Neuropeptide families: evolutionary perspectives. Reg. Peptides, 73, 1-33 https://doi.org/10.1016/S0167-0115(97)01073-2
  15. Iijima, N., N. Tanimoto, Y. Emoto, Y. Morita, K. Uematsu, T. Murakami and T. Nakai. 2003. Purification and characterization of three isoforms of chrysophsin, a novel antimicrobial peptide in the gills of the red sea bream, Chrysophrys major. , 270, 675-686 https://doi.org/10.1046/j.1432-1033.2003.03419.x
  16. Irwin, D.M. and J. Wong. 1995. Trout and chicken pro glucagon : alternative splicing generates mRNA trans cripts encoding glucagon-like peptide. Mol. Endocrinol., 9, 267-277 https://doi.org/10.1210/me.9.3.267
  17. Iwakiri, M., A. Sugiyama, T. Ikeda, Y. Muneoka and I Kubota. 1990. A novel oxytocin-like peptide isolated form the neural complexes of tunicate, Styele plicata. Zool. Sci., 7, 1035-1041
  18. Jensen, J. and J.M. Colon. 1992. Substance-P-related and neurokinin-A related peptides from the brain of the cod and trout. Eur. J. Biochem., 206, 659-664
  19. Kang, D.G., C.K. Yun and H.S. Lee. 2003. Screening and comparison of antioxidant activity of solvent extracts of herbal medicines used in Korea. J. Ethnopharm., 87, 231-236 https://doi.org/10.1016/S0378-8741(03)00142-9
  20. Kim, W.G., J.P. Kim, H. Koshino, K. Shin-Ya, H. Seto and I.D. Yoo. 1997. Benzastatin E, F, and G: new indoline alkaloids with neronal cell protecting activity form Streptomyces nitrosporesus. Tetrahedron, 53, 4309-4316 https://doi.org/10.1016/S0040-4020(97)00157-9
  21. Koshino, H., I.K. Lee, J.P. Kim, W.G. Kim, J. Uzawa. and I.D. Yoo. 1996. Agrocyvennie, novel class alkaloid from the Korean mushroom. Phytochemistry, 41, 213-216 https://doi.org/10.1016/0031-9422(95)00609-5
  22. Krieger, D.T. 1983. Brain peptide: What, where, and why? Science, 222, 975-985 https://doi.org/10.1126/science.6139875
  23. Krieger, D.T. 1986. An overview of neuropeptides. In: Neuropeptide in Neurologic and Psychiatric Disease, Martin, J.B. and J.D. Barchas eds., Raven Press, New York, pp 1-33
  24. Lauth, X., H. Shike, J.C. Burns, M.E. Westerman, V.E. Ostland, J.M. Carlberg, J.C.V. Olst, V. Niset, S.W. Taylor, C. Shimizu and P. Bulet. 2002. Discovery and characterization of two isofbrms of moronecidin, a novel antimicrobial peptide from hybrid striped bass. J. Biol. Chem., 277, 5030-5039 https://doi.org/10.1074/jbc.M109173200
  25. McMasters, D., Y. Kobayashi and K. Lederis. 1992. A vasotocin-like peptide in Aplysia kurodai ganglia: HPLC and RIA evidence for its identity with Lysconopressin-G. Peptides, 13, 413-422 https://doi.org/10.1016/0196-9781(92)90069-F
  26. McRory, J. and N. M. Sherwood. 1997. Two protochordate genes encode pituitary adenylate cyclase-activating polypeptide and related family members. , 138, 2380-2390 https://doi.org/10.1210/en.138.6.2380
  27. Moore, K. S., S. Wehrli, H. Roder, M. Rogers, J. N. Forrest, Jr., D. McCrimmon and M. Zasloff, 1993. Squlalamine: An aminosterol antibiotic from the shark. Proc. Natl. Acad. USA., 90, 1354-1358 https://doi.org/10.1073/pnas.90.4.1354
  28. Park, J.H., K.C. Kang, S.B. Baek, Y.H. Lee and K.S. Rhee. 1991. Separation of antioxidant compounds from edible marine algae. Kor. J. Food Sci. Technol., 23, 256-261
  29. Parker, D.B., I.R. Coe and G.H. Dixon. 1993. Two salmon neuropeptides encoded by one brain cDNA are structurally related to members of the glucagon superfamily. Eur. J. Biochem., 215, 439-448 https://doi.org/10.1111/j.1432-1033.1993.tb18051.x
  30. Rice-Evans, C.A. and N.J. Miller. 1996. Antioxidant activities of flavonoids as bioactive compounds of food. Biochem. Soc. Trans., 24, 790-795 https://doi.org/10.1042/bst0240790
  31. Rinehart, K.L., Jr. 1983. Biologically active compounds from marine source. Mar. Chem., 12, 229-239 https://doi.org/10.1016/0304-4203(83)90091-9
  32. Rovinette, D., S. Wada, T. Arroll, M.G. Levy, W.L. Miller and E.J. Noga. 1998. Antimicrobial activity in the skin of the channel catfish lctalurus punctatus: charasterization of broad-spectrum histone-like antimicrobial proteins. Cell. Mol. Life Sci., 54, 467-475 https://doi.org/10.1007/s000180050175
  33. Salzet, M., P. Bulet, A. Van Dorsselaer and J. Malecha. 1993. Isolation, structural characterization and biological function of lysine-conopressin in the central nervous system of the pharyngvdellid leech Erpobdella otoculata. Eur. J. Biochem., 217, 879-903
  34. Shin, M.J., E.J. Kim, C.H. Kim, H.J. Go, I.H. Kim, H.S. Ryu, M.D. Huh, J.K. Chung and N.G. Park. 2003. Purification of a bradykinin-related peptide from the skin of hagfish, Eptatretus burgeri. J. Kor. Fish. Soc., 36, 30-34
  35. Silphaduang, U. and E.J. Noga. 2001. Peptides antibiotics in mast cells of fish. Nature, 414, 268-289 https://doi.org/10.1038/35104690
  36. Waugh, D., V. Bondareva, Y. Rusakov, C. Bjenning, P.F. Nielsen and J.M. Conlon. 1995. Tachykinins with unusual structural features form a urodele, the amphiuma, an elasmobrach, the hammergea shark, and an agnathan, the river lamprey. Peptides, 16, 615-621 https://doi.org/10.1016/0196-9781(95)00010-H
  37. Waugh, D., Y. Wang, N. Hazon, R.J. Balment and J.M. Conlon. 1993. Primary structures an biological activities of substance-P-related peptides form the brain of the dogfish, Sliohinus canicula. Eur. J. Biochem., 214, 469-474 https://doi.org/10.1111/j.1432-1033.1993.tb17943.x
  38. Zadina, J.E., W.A. Banks and A.J. Kastin. 1986. Central nervous system effects of peptides, 1980-1985: a cross-listing of peptides and their central actions from the first six years of the journal peptides. Peptides, 7, 497-537