• Journal of Microbiology and Biotechnology
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• 제23권9호
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• pp.1229-1243
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• 2013

In this paper, an air isolate (NITT6L) has been screened based on hemolytic activity, emulsification activity, drop collapsing test, and oil displacement test, as well as lipase activity. It was found that strain NITT6L was able to reduce the surface tension of the medium from 61.5 to 39.83 mN/m and could form stable emulsions with tested vegetable oils. Morphological, biochemical, 16S rRNA sequencing analyses, and fatty acid methyl ester analysis using gas chromatography confirmed that the air isolate under study was Pseudomonas aeruginosa. Characterization of the biosurfactant using agar double diffusion assay revealed that the biosurfactant was anionic in nature, and CTAB-methylene blue assay and Molisch test revealed its glycolipid nature. The FT-IR spectrum confirmed that the crude biosurfactant was a rhamnolipid. Using unoptimized medium containing sucrose as the carbon source, the isolate was found to produce 0.3 mg/ml of rhamnolipid in batch cultivation (shake flask) at $37^{\circ}C$ and pH 7. Optimization of the medium components was carried out using design of experiments and the yield of rhamnolipid has been enhanced to 4.6 mg/ml in 72 h of fermentation.

• 한국지반공학회지:지반
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• 제3권3호
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• pp.7-20
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• 1987

교번 삼축 압축시험, 교번 전단응력 시험, 액상화 시험 및 진동 테이블 시험 등을 하기 위하여 제작되는 공시체로서 잘 다져진 모래를 사용하게 되며 진동에 의해 다져진 모래의 공시체가 균일한 밀도를 갖는다는 가정하에 상기한 실험을 하여 왔다. 지금까지 이러한 가정에 대한 연구가 충분히 되어 있지 않았으며 신뢰도 높은 실험을 하기 위하여는 공시체내에 모래의 밀도가 균일한 공시체를 제작하는 것이 중요하다. 이 연구에서는 진동에 의해서 모래 공시체를 제작할 경우 공시체내에 밀도의 변화가 발생하는지 여부와 각 진동인자가 밀도변화에 미치는 영향을 조사하였다. 이 연구 결과 진동 전에 균일한 밀도 를 갖는 공시체가 진동 후엔 공시체의 높이에 따라 상당한 밀도의 변화가 발생하였음을 보였으며 이러한 현상은 과재 하중, 입도 및 진동가속도의 크기에 따라 상당한 영향을 받는 것으로 밝혀졌다.

• Structural Engineering and Mechanics
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• 제24권3호
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• pp.275-290
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• 2006

In this paper, the performance of a tuned liquid damper (TLD) in suppressing the seismic response of buildings is investigated with shake table testing of a four-story steel frame model that rests on pile foundation. The model tests were performed in three phases with the steel frame structure alone, the soil and pile foundation system, and the soil-foundation-structure system, respectively. The test results from different phases were compared to study the effect of soil-structure interaction on the efficiency of a TLD in reducing the peak response of the structure. The influence of a TLD on the dynamic response of the pile foundation was investigated as well. Three types of earthquake excitations were considered with different frequency characteristics. Test results indicated that TLD can suppress the peak response of the structure up to 20% regardless of the presence of soils. TLD is also effective in reducing the dynamic responses of pile foundation.

• Earthquakes and Structures
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• 제6권5호
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• pp.539-560
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• 2014

Shaking tables are devices for testing structures or structural components models with a wide range of synthetic ground motions or real recorded earthquakes. They are essential tools in earthquake engineering research since they simulate the effects of the true inertial forces on the test specimens. The destructive earthquakes that occurred at the north part of Algeria during the period of 1954-2003 resulted in an initiative from the Algerian authorities for the construction of a shaking simulator at the National Earthquake Engineering Research Center, CGS. The acceleration tracking performance and specifically the inability of the earthquake simulator to accurately replicate the input signal can be considered as the main challenge during shaking table test. The objective of this study is to validate the uni-axial sinusoidal performances curves and to assess the accuracy and fidelity in signal reproduction using the advanced adaptive control techniques incorporated into the MTS Digital controller and software of the CGS shaking table. A set of shake table tests using harmonic and earthquake acceleration records as reference/commanded signals were performed for four test configurations: bare table, 60 t rigid mass and two 20 t elastic specimens with natural frequencies of 5 Hz and 10 Hz.

• Steel and Composite Structures
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• 제24권4호
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• pp.441-453
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• 2017

In Y-shaped eccentrically braced frame fabricated with high strength steel (Y-HSS-EBF), link uses conventional steel while other structural members use high strength steel. Cyclic test for a 1:2 length scaled one-bay and one-story Y-HSS-EBF specimen and shake table test for a 1:2 length scaled three-story Y-HSS-EBF specimen were carried out to research the seismic performance of Y-HSS-EBF. These include the failure mode, load-bearing capacity, ductility, energy dissipation capacity, dynamic properties, acceleration responses, displacement responses, and dynamic strain responses. The test results indicated that the one-bay and one-story Y-HSS-EBF specimen had good load-bearing capacity and ductility capacity. The three-story specimen cumulative structural damage and deformation increased, while its stiffness decreased. There was no plastic deformation observed in the braces, beams, or columns in the three-story Y-HSS-EBF specimen, and there was no danger of collapse during the seismic loads. The designed shear link dissipated the energy via shear deformation during the seismic loads. When the specimen was fractured, the maximum link plastic rotation angle was higher than 0.08 rad for the shear link in AISC341-10. The Y-HSS-EBF is a safe dual system with reliable hysteretic behaviors and seismic performance.

• Structural Monitoring and Maintenance
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• 제7권4호
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• pp.319-344
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• 2020

Inverse analysis of non-linear reinforced concrete bridge pier using recursive Gaussian filtering for in-situ condition assessment is the main theme of this work. For this purpose, minimum variance unbiased estimation using unscented sigma points is adopted here. The uniqueness of this inverse analysis lies in its approach for strain based updating of engineering demand parameters, where appropriate bound and constrained conditions are introduced to ensure numerical stability and convergence. In this analysis, seismic input is also identified, which is an added advantage for the structures having no dedicated sensors for earthquake measurement. First, the proposed strategy is tested with a simulated example whose hysteretic properties are obtained from the slow-cyclic test of a frame to investigate its efficiency and accuracy. Finally, the experimental test data of a full-scale bridge pier is used to study its in-situ condition in terms of Park & Ang damage index. Overall the study shows the ability of the augmented minimum variance unbiased estimation based recursive time-marching algorithm for non-linear system identification with the aim to estimate the engineering damage parameters that are the fundamental information necessary for any future decision making for retrofitting/rehabilitation.

• 생약학회지
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• 제15권2호
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• pp.61-73
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• 1984

To investigate antitumor component of Lyophyllum decastes, the aqueous extract of its shake-cultured mycelia was subjected to antitumor test against sarcoma 180 cells implanted in ICR mice. The extract showed an inhibition ratio of 65.4% and was found to consist of a polysaccharide moiety and a protein moiety. After purification with DEAE-Sephadex A-50 ion exchange chromatography, Fraction D showed the highest inhibition ratio of 75.7%. The antitumor constituent was examined for immunoaccelerating activity and was found to increase macrophage accumulation in the peritoneal cavity and plaque forming cells of the spleen cells. It was named lyophyllan after the genus name.

• 한국유체기계학회 논문집
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• 제2권2호
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• pp.32-38
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• 1999

An experimental study is performed to investigate the frequency effects of the excitation force on the linear stiffness and damping coefficients of a LOP (load on pad) type five-pad tilting pad journal bearing with the diameter of 300.91 mm and the length of 149.80 mm. The main parameter of interest in the present work is excitation frequency to shake the test bearing. The excitation frequency is controlled independently, using orthogonally mounted hydraulic exciters. The relative movement between the bearing and shaft, and the acceleration of the bearing casing are measured as a function of excitation frequency using the different values of bearing load and shaft speed. Measurements show that the variation of excitation frequency has quite a little effect on both stiffness and damping coefficients. Both direct stiffness and damping coefficients in the direction of bearing load decrease by the increase of shaft speed, but increase with the bearing load.

• 유체기계공업학회:학술대회논문집
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• 유체기계공업학회 1998년도 유체기계 연구개발 발표회 논문집
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• pp.173-179
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• 1998

An experimental study is performed to investigate the frequency effects of the excitation force on the linear stiffness and damping coefficients of a LOP (load on pad) type five-pad tilting pad journal bearing with the diameter of 300.91 mm and the length of 149.80 mm. The main parameter of interest in the present work is excitation frequency to shake the test bearing. The excitation frequency is controlled independently, using orthogonally mounted hydraulic exciters. The relative movement between the bearing and shaft, and the acceleration of the bearing casing are measured as a function of excitation frequency using the different values of bearing load and shaft speed. Measurements show that the variation of excitation frequency has quite a little effect on both stiffness and damping coefficients. Both direct stiffness and damping coefficients in the direction of bearing load decrease by the increase of shaft speed, but increase with the bearing load.

• Smart Structures and Systems
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• 제14권3호
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• pp.445-468
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• 2014

The objective in this research is to determine the feasibility of using changes on the dynamic properties of a reinforced concrete (RC) structure to identify different levels of seismic induced damage. Damping ratio and natural frequency changes in a RC bridge column are analyzed using different signal processing techniques like Hilbert Transforms, Random Decrement and Wavelet Transforms. The data used in the analysis was recorded during a full-scale RC bridge column shake table test. The structure was subjected to ten earthquake excitations that induced different levels of inelastic demand on the column. In addition, low-intensity white noises were applied to the column in-between earthquakes. The results obtained show that the use of the damping ratio and natural frequency of vibration as damage indicators is arguable.