Browse > Article
http://dx.doi.org/10.4014/jmb.1212.12031

Biosurfactant Production from Novel Air Isolate NITT6L: Screening, Characterization and Optimization of Media  

Vanavil, B. (Department of Chemical Engineering, National Institute of Technology)
Perumalsamy, M. (Department of Chemical Engineering, National Institute of Technology)
Rao, A. Seshagiri (Department of Chemical Engineering, National Institute of Technology)
Publication Information
Journal of Microbiology and Biotechnology / v.23, no.9, 2013 , pp. 1229-1243 More about this Journal
Abstract
In this paper, an air isolate (NITT6L) has been screened based on hemolytic activity, emulsification activity, drop collapsing test, and oil displacement test, as well as lipase activity. It was found that strain NITT6L was able to reduce the surface tension of the medium from 61.5 to 39.83 mN/m and could form stable emulsions with tested vegetable oils. Morphological, biochemical, 16S rRNA sequencing analyses, and fatty acid methyl ester analysis using gas chromatography confirmed that the air isolate under study was Pseudomonas aeruginosa. Characterization of the biosurfactant using agar double diffusion assay revealed that the biosurfactant was anionic in nature, and CTAB-methylene blue assay and Molisch test revealed its glycolipid nature. The FT-IR spectrum confirmed that the crude biosurfactant was a rhamnolipid. Using unoptimized medium containing sucrose as the carbon source, the isolate was found to produce 0.3 mg/ml of rhamnolipid in batch cultivation (shake flask) at $37^{\circ}C$ and pH 7. Optimization of the medium components was carried out using design of experiments and the yield of rhamnolipid has been enhanced to 4.6 mg/ml in 72 h of fermentation.
Keywords
Biosurfactant; bioprocess design; fermentation; optimization; modeling; response surface methodology;
Citations & Related Records
연도 인용수 순위
  • Reference
1 Satpute SK, Banat IB, Dhakephalkar PK, Banpurkar AG, Chopade BA. 2010. Biosurfactants, bioemulsifiers and exopolysaccharides from marine microorganisms. Biotechnol. Adv. 28: 436-450.   DOI   ScienceOn
2 Santos SC, Fernandez LG, Rossi-Alva JC, Roque MRA. 2010. Evaluation of substrates from renewable-resources in biosurfactants production by Pseudomonas strains. Afr. J. Biotechnol. 9: 5704-5711.
3 Santos AS, Sampaio AW, Vasquez GS, Santa-Anna LM, Pereira N, Freire MG. 2002. Evaluation of different carbon and nitrogen sources in production of rhamnolipids by a strain of Pseudomonas aeroginosa. Appl. Biochem. Biotechnol. 98: 1025-1035.
4 Santos LG, Kappeli O, Fiechter A. 1984. Pseudomonas aeruginosa biosurfactant production in continuous culture with glucose as carbon source. Appl. Environ. Microbiol. 48: 301-305.
5 Santa Anna LM, Sebastian GV, Menezes EP, Alves TLM, Santos AS, Pereira Jr N, et al. 2002. Production of biosurfactants from Pseudomonas aeruginosa PA1 isolated in oil environments. Braz. J. Chem. Eng. 19: 159-166.   DOI   ScienceOn
6 Silva SNRL, Farias CBB, Rufino RD, Luna JM, Sarubbo LA. 2010. Glycerol as substrate for the production of biosurfactant by Pseudomonas aeruginosa UCP0992. Colloids Surf. B Biointerfaces 79: 174-183.   DOI   ScienceOn
7 Stanbury PF, Whitaker A, Hall SJ. 1995. Principles of Fermentation Technology, 2nd Ed. Butterworth-Heinemann, Burlington, MA.
8 Lotfabad TB, Shourian M, Roostaazad R, Najafabadi AR, Adelzadeh MR, Noghabi KA. 2009. An efficient biosurfactantproducing bacterium, Pseudomonas aeruginosa MR01, isolated from oil excavation areas in south of Iran. Colloids Surf. B Biointerfaces 69: 183-193.   DOI   ScienceOn
9 Techaoei S. 2011. Screening, characterization and stability of biosurfactant produced by Pseudomonas aeruginosa SCMU106 isolated from soil in Northern Thailand. Asian J. Biol. Sci. 4: 340-351.   DOI
10 Thaniyavarn J, Chongchin A, Wanitsuksombut N, Thaniyavarn S, Pinphanichakarn P, Leepipatpiboon N, et al. 2006. Biosurfactant production by Pseudomonas aeruginosa A41 using palm oil as carbon source. J. Gen. Appl. Microbiol. 52: 215-222.   DOI   ScienceOn
11 Liu T, Hou J, Zuo Y, Bi S, Jing J. 2011. Isolation and characterization of a biosurfactant producing bacterium from Daqing oil-contaminated sites. Afr. J. Microbiol. Res. 5: 3509-3514.
12 Makkar RS, Swaranjit R, Cameotra SS, Banat IM. 2011. Advances in utilization of renewable substrates for biosurfactant production. AMB Express 1: 5-10.   DOI   ScienceOn
13 Makkar RS, Cameotra SS. 2002. An update on the use of unconventional substrates for biosurfactant production and their new applications. Appl. Microbiol. Biotechnol. 58: 428-434.   DOI   ScienceOn
14 Marsudi S, Unno H, Hori K. 2008. Palm oil utilization for the simultaneous production of polyhydroxyalkanoates and rhamnolipids by Pseudomonas aeruginosa. Appl. Microbiol. Biotechnol. 78: 955-961.   DOI
15 Medina-Moreno SA, Jimenez-Islas D, Gracida-Rodriguez JN, Gutierrez-Rojas M, Diaz-Ramirez IJ. 2011. Modeling rhamnolipids production by Pseudomonas aeruginosa from immiscible carbon source in a batch system. Int. J. Environ. Sci. Technol. 8: 471-482.   DOI   ScienceOn
16 Noudeh GD, Noodeh AD, Moshafi MH, Behravan E, Afzadi MA, Sodagar M. 2010. Investigation of cellular hydrophobicity and surface activity effects of biosynthesed biosurfactant from broth media of PTCC 1561. Afr. J. Microbiol. Res. 4: 1814-1822.
17 Pinzon MN, Ju LK. 2009. Improved detection of rhamnolipid production using agar plates containing methylene blue and cetyltrimethyl ammonium bromide. Biotechnol. Lett. 31: 1583-1588.   DOI
18 Mulligan CN, Gibbs BF. 2004. Types, production and applications of biosurfactants. Proc. Indian Natl. Sci. Acad. 1: 31-55.
19 Muthusamy K, Gopalakrishnan S, Ravi TK, Sivachidambaram P. 2008. Biosurfactants: properties, commercial production and application. Curr. Sci. 94: 736-747.
20 Rajan A, Soban Kumar DR, Nair JA. 2011. Isolation of a novel alkaline lipase producing fungus Aspergillus fumigatus MTCC 9657 from aged and crude rice bran oil and quantification by HPTLC. Int. J. Biol. Chem. 5: 116-126.   DOI
21 Robert M, Mercade ME, Bosch MP, Parra JL, Espuny MJ, Manresa MA, et al. 1989. Effect of the carbon source on biosurfactant production by Pseudomonas aeruginosa 44T. Biotechnol. Lett. 11: 871-874.   DOI
22 Rodrigues L, Banat IM, Teixeira J, Oliveira R. 2006. Biosurfactants: potential applications in medicine. J. Antimicrob. Chemother. 57: 609-618.   DOI   ScienceOn
23 Roldan-Carrillo T, Martinez-Garcia X, Zapata-Penasco I, Castorena-Cortes G, Reyes-Avila J, Mayol-Castillo M, et al. 2011. Evaluation of the effect of nutrient ratios on biosurfactant production by Serratia marcescens using a Box-Behnken design. Colloids Surf. B Biointerfaces 86: 384-389.   DOI   ScienceOn
24 Banat IM, Franzetti A, Gandolfi I, Bestetti G, Martinotti MG, Fracchia L, et al. 2010. Microbial biosurfactants production, applications and future potential. Appl. Microbiol. Biotechnol. 87: 427-444.   DOI   ScienceOn
25 Anastasia A, Pantazaki AA, Dimopoulou MI, Simou OM, Pritsa AA. 2010. Sunflower seed oil and oleic acid utilization for the production of rhamnolipids by Thermus thermophilus HB8. Appl. Microbiol. Biotechnol. 88: 939-951.   DOI
26 Cho SK, Shim SH, Park KR, Choi SH, Lee S. 2006. Purification and characterization of a biosurfactant produced by Pseudomonas sp. G11 by asymmetrical flow field-flow fractionation (AsFlFFF). Anal. Bioanal. Chem. 386: 2027-2033.   DOI
27 Abdel-Mawgoud AM, Lepine F, Dezie E. 2010. Rhamnolipids: diversity of structures, microbial origins and roles. Appl. Microbiol. Biotechnol. 86: 1323-1336.   DOI   ScienceOn
28 Abouseoud M. 2008. Evaluation of different carbon and nitrogen sources in production of biosurfactant by Pseudomonas fluorescens. Desalination 223: 143-151.   DOI   ScienceOn
29 Carrillo PG, Mardaraz C, Pitta-Alvarez SJ, Giulietti AM. 1996. Isolation and selection of biosurfactant-producing bacteria. World J. Microbiol. Biotechnol. 12: 82-84.   DOI   ScienceOn
30 Darvishi P, Ayatollahi S, Mowla D, Niazi A. 2011. Biosurfactant production under extreme environmental conditions by an efficient microbial consortium, ERCPPI-2. Colloids Surf. B Biointerfaces 84: 292-300.   DOI   ScienceOn
31 Dubey K, Juwarkar A. 2001. Distillery and curd whey wastes as viable alternative sources for biosurfactant production. World J. Microbiol. Biotechnol. 17: 61-69.   DOI   ScienceOn
32 Kiran GS, Hema TA, Gandhimathi R, Selvin J, Thomas TA, Ravji TR, et al. 2009. Optimization and production of a biosurfactant from the sponge-associated marine fungus Aspergillus ustus MSF3. Colloids Surf. B Biointerfaces 73: 250-256.   DOI   ScienceOn
33 Dubois M, Gills KA, Hamilton JK, Rebers PA, Smith F. 1956. Colorimetric method for determination of sugar and related substances. Anal. Chem. 28: 350-356.   DOI
34 Gautam KK, Tyagi VK. 2006. Microbial surfactant: a review. J. Oleo Sci. 55: 155-166.   DOI   ScienceOn
35 Khoramnia A, Lai OM, Ebrahimpour A, Tanduba CJ, Voon TS, Mukhlis S. 2010. Thermostable lipase from a newly isolated Staphylococcus xylosus strain; process optimization and characterization using RSM and ANN. Electron. J. Biotechnol. 13: 1-16.
36 Thaniyavarn J, Chianguthai T, Sangvanich P, Roongsawang N, Washio K, Morikawa M, et al. 2008. Production of sophorolipid biosurfactant by Pichia anomala. Biosci. Biotechnol. Biochem. 72: 2061-2068.   DOI   ScienceOn
37 Laith AA, Zaliha RN, Rahman RA, Basri M, Salleh AB. 2007. Microbial surfactant. Asia Pac. J. Mol. Biol. Biotechnol. 15: 99-105.
38 Leitermann F, Syldatk C, Hausmann R. 2008. Fast quantitative determination of microbial rhamnolipids from cultivation broths by ATR-FTIR spectroscopy. J. Biol. Eng. 2: 13-21.   DOI   ScienceOn
39 Lindum PW, Anthoni U, Christophersen C, Eberl L, Molin S, Givskov M. 1998. N-Acyl-L-homoserine lactone autoinducers control production of an extracellular lipopeptide biosurfactant required for swarming motility of Serratia liquefaciens MG1. J. Bacteriol. 180: 6384-6388.
40 Zhao J, Wang Y, Chu J, Zhang S, Zhuang Y, Yuan Z. 2008. Statistical optimization of medium for the production of pyruvate oxidase by the recombinant Escherichia coli. J. Ind. Microbiol. Biotechnol. 35: 257-262.   DOI
41 Viramontes-Ramos S, Portillo-Ruiz MC, Ballinas-Casarrubias ML, Torres-Munoz JV, Rivera-Chavira BE, Navarez-Moorillon GV. 2010. Selection of biosurfactant/bioemulsifier-producing bacteria from hydrocarbon contaminated soil. Braz. J. Microbiol. 41: 668-675.   DOI   ScienceOn
42 Wang Q, Fang X, Bai B, Liang X, Shuler PJ, Goddard III WA, et al. 2007. Engineering bacteria for production of rhamnolipid as an agent for enhanced oil recovery. Biotechnol. Bioeng. 98: 842-853.   DOI   ScienceOn
43 Xu Q, Nakajima M, Liu Z, Shiina T. 2011. Biosurfactants for microbubble preparation and application. Int. J. Mol. Sci. 12: 462-475.   DOI   ScienceOn
44 Zhu Y, Gan JJ, Zhang G, Yao B, Zhu W, Qin M. 2007. Reuse of waste frying oil for production of rhamnolipids using Pseudomonas aeruginosa zju.u1M. J. Zhejiang Univ. Sci. 8: 1514-1520.   DOI