• Safety and Health at Work
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• v.11 no.2
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• pp.187-192
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• 2020

Background: This study investigated the relationship between verbal aggression against school teachers and upper extremity (neck, shoulder, upper limb, and/or upper back) musculoskeletal pain. Methods: This was a cross-sectional study of 525 elementary school teachers from Jaboatão dos Guararapes, Northeast Brazil. Results: The prevalence of upper extremity musculoskeletal pain among teachers who reported verbal aggression in the past six months (67.7%) was higher than that among those who did not report verbal aggression (51.7%): (prevalence ratio = 1.21; 95% confidence interval = 1.04-1.40). The prevalence of upper extremity musculoskeletal pain was associated with verbal aggression, sex, and common mental disorders, controlled by skin color, age, monthly income, teachers' education, years working as a teacher, workload, and obesity. Furthermore, the measure of the association between verbal aggression and upper extremity musculoskeletal pain was modified by sex and common mental disorders, considered altogether. Teachers who suffered verbal aggression, of the feminine sex, and also having common mental disorders reported high prevalence (85.4%) of upper extremity musculoskeletal pain. Conclusion: The association between verbal violence in the school and complaints of upper extremity musculoskeletal pain was strong and modified by teachers' sex and common mental disorders.

• Toxicological Research
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• v.33 no.4
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• pp.315-323
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• 2017

Preconceptual sex selection is still a highly debatable process whereby X- and Y-chromosome-bearing spermatozoa are isolated prior to fertilization of the oocyte. Although various separation techniques are available, none can guarantee 100% accuracy. The aim of this study was to separate X- and Y-chromosome-bearing spermatozoa using methods based on the viability difference between the X- and Y-chromosome-bearing spermatozoa. A total of 18 experimental semen samples were used, written consent was obtained from all donors and results were analysed in a blinded fashion. Spermatozoa were exposed to different pH values (5.5, 6.5, 7.5, 8.5, and 9.5), increased temperatures ($37^{\circ}C$, $41^{\circ}C$, and $45^{\circ}C$) and ROS level ($50{\mu}M$, $750{\mu}M$, and $1,000{\mu}M$). The live and dead cell separation was done through a modified swim-up technique. Changes in the sex-chromosome ratio of samples were established by double-label fluorescent in situ hybridization (FISH) before and after processing. The results indicated successful enrichment of X-chromosome-bearing spermatozoa upon incubation in acidic media, increased temperatures, and elevated $H_2O_2$. This study demonstrated the potential role for exploring the physiological differences between X-and Y-chromosome-bearing spermatozoa in the development of preconceptual gender selection.

• YAKHAK HOEJI
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• v.57 no.5
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• pp.330-336
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• 2013

"The Act on Medication Treatment of Sexual Impulse of Sex Offenders" known as chemical castration has been effective since July 2011 in Korea. According to the law, monitoring of male sex hormone in urine is enforced to request National Forensic Service more than once a month after injection of medicine designed to reduce sex impulse. We established a rapid and sensitive method for the monitoring of testosterone (T) and epitestosterone (E) in human urine by liquid chromatography with tandem mass spectrometry (LC-MS/MS). Three mL of urine was pretreated by solid-phase extraction for purification and performed enzymatic hydrolysis. The pretreated samples were extracted twice with 2 ml of ethyl acetate and n-hexane (2 : 3). The separation was applied on Thermo Hypersil GOLD C18 column ($1.9{\mu}m$, $100{\times}2.1mm$). A gradient elution of methanol and water of 0.1% formic acid were used as mobile phase and the retention time was less than 10 min. LC-MS/MS system coupled with an electrospray ionization source was performed in multiple reaction monitoring mode. The transitions of the analytes executed as following: m/z $289{\rightarrow}97$, 109 for T and E, m/z $292{\rightarrow}109$ for $T-d_3$ and $E-d_3$ as internal standards. The validation results of the method were satisfactory. The limits of detection were 0.05 ng/ml and the limits of quantification were 0.1 ng/ml. This method was successfully applied to real human urine sample. The developed method will be useful for monitoring T/E ratio in urine of sex offenders.

• Journal of Ecology and Environment
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• v.44 no.3
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• pp.143-154
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• 2020

Background: Korean wild boars (Sus scrofa coreanus Heude), because of their adaptability, are a widespread large mammal; however, they sometimes cause problems by invading farms and eating the crops, creating insufficiencies of some foods in South Korea. To understand the diet composition of Korean wild boars according to sex and body size, we collected their feces from Mt. Jeombongsan, Seoraksan National Park, South Korea. The sizes of fecal samples were measured, and genomic DNA was extracted from the samples. We amplified specific loci targeting plants (rbcL and trnL) and animals (COI) to detect the food sources of this omnivore and amplified the ZF and SRY regions to determine the sex. Results: In the wild boar feces, Rosaceae and Bryophyte were the most frequently detected plant food sources at the family level and Diptera and Haplotaxida were the most frequently detected animal food sources at the order level. As a result of sex determination, the sex ratio of wild boars collected in the Mt. Jeombongsan area was approximately 1:1. Our result suggested that there is no significant difference between the diet composition of male and female boars. Based on the average cross-sectional area of the feces, the top 25% were classified into the large body size group and the bottom 25% were classified into the small body size group. The large body size group mainly preferred Actinidiaceae, and the small body size group most frequently consumed Fagaceae. The diet of the large body size group was more diverse than the small body size group. Conclusions: Our results showed that the wild boars preferred Rosaceae, especially Sanguisorba and Filipendula, as plant food sources, and Diptera and Coleoptera of Insecta as animal food sources. Based on the results, the dietary preferences of wild boar appear to be distinguished by not their sex but their body size. Our study could help to elucidate the feeding ecology and population structure of wild boar, as well as address conservation and management issues.

• Biomolecules & Therapeutics
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• v.8 no.1
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• pp.38-43
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• 2000

Several studies have shown that the stomach has sufficient alcohol dehydrogenase (ADH) activity to metabolize some amount of orally administered alcohol and the sex-related differences in the first-pass metabolism of alcohol might be associated with differences in the activity of gastric ADH(GADH). The aim of this study was to asses the sex-related differences in GADH in 48 male and 48 female Sprague-Dawley rats aged 1, 4, 10, 15, 20, and 30 weeks which each aged group had same sex ratio. The GADH activity was determined spectrophotometrically at 37$^{\circ}C$. The formation of NADH was monitored at 340nm for 10 minutes in the 1 ml of reaction mixture (0.5 M of Tris-HCl, pH 7.2 + 1.5 M of ethanol + 2.8 mM of NAD + 30 $\mu$l gastric mucosal supernatant). The GADH activity (nM of NADH/min/mg of cytosolic protein) was calculated using molecular extinction coefficient of 6.22 $\textrm{cm}^2$/$\mu$M for NADH. The GADH activities were 2.94$\pm$0.82 (n=48) in female rats and 3.34$\pm$2.17 (n=48) in male rats and had not significant difference between sex. However, the GADH activities were significantly (p<0.01) higher in female (1.91$\pm$0.59 and 3.30$\pm$0.49) than in male (0.68$\pm$0.43 and 1.92$\pm$0.81) of 1 and 4 weeks rats. However, it was significantly (p<0.05) higher in male (6.48$\pm$1.81, 3.65$\pm$1.04 and 5.13$\pm$1.30) than in female (4.23$\pm$1.23, 2.18$\pm$0.77 and 2.56$\pm$0.93) of 10, 20 and 30 weeks rats, respectively. Therefore, the results suggested that sex-related differences of the GADH activities in same aged rats were existed by age.

• Korean journal of applied entomology
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• v.44 no.1 s.138
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• pp.31-36
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• 2005

Adoxophyes spp. are the major rests of a pear. The larvae attack both leaves and fruits. (Z)-9-tetradecenyl acetate (Z9-14:Ac), (Z)-11-tetradecenyl acetate (Z11-14:Ac), (E)-11-tetradecenyl acetate (E11-14:Ac) and 10-methyldodecyl acetate (10me-12:Ac) have been reported as the sex pheromone components of the genus Adoxophyes. Our objective was to determine the difference in sex pheromone composition of three different A. spp. populations each from Cheonan, Sangju, and Naju area orchards in Korea. Gas chromatography (GC) analyses of pheromone gland extracts of virgin females confirmed the presence of two compounds (Z9-14:Ac and Z11-14:Ac) in Cheonan and Sangju populations, and four compounds (Z9-14:Ac, Z11-14:Ac, E11-14:Ac and tome-12:Ac) in Naju population. The Z9-14:Ac and Z11-14:Ac were detected in the ratio of 80:20 in the Cheonan population and 3:97 in the Sangju population. Females of Naju population produced sex pheromone blend consisting of Z9-14:Ac, Z11-14:Ac, E11-14:Ac and 10me-12:Ac at a ratio of 31:62:6:1. Field trapping tests in pear orchards with Z9-14:Ac and Z11-14:Ac indicated that maximum captures of the male were obtained with traps baited by 80:20 in Cheonan, 10:90 in Sangju, and 30:70 in Naju. These results suggest that there are remarkable geographical variations in the sex pheromone composition of A. spp. in pear orchards in Korea, and taxonomic classification of these species must be carefully assessed.

• Korean Journal of Animal Reproduction
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• v.24 no.3
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• pp.299-309
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• 2000

This study was performed 1) to establish the optimal PCR condition of sex determination in Hanwoo IVM/IVF embryos, 2) to examine the sex determination and sex ratio to the developmental stages of Hanwoo IVM/IVF embryos by two-step PCR method. The sexing of bovine IVF embryos were accurately determined by PCR methods using Y chromosome specific DNA primer(BOV 97M, 141bp) and bovine specific DNA primer(216bp). The fregment size were shown at 141 and 216 base pairs(bp) in male, and 216 bp in female. Two-steps PCR method in which the samples were amplified by 15 cycles with Y chromosome specific DNA primer and then amplified by additional 30 cycles with bovine specific DNA primer was effective in the sexing of bovine IVF embryos. The zona-free embryos were more effective than zona-intact embryos in bovine IVF embryo sexing. The appearance of Y chromosome specific band was 45.2% in embryos treated with protease K and 53.3% in embryos treated with freezing and thawing repeatedly. The optimun volume of DNA for sexing of Hanwoo IVF embryos were 2 to 10 $\mu$1 in Zona-free embryos and 12 to 13 $\mu$1 in zona-intact embryos. The sexing rate of bovine IVF embryos by PCR was 96.0% and questionable rate not identified sex was 4.0%, respectively. Among the sexed embryos, the percentage of male and female was 49.7% and 46.3%, respectively, the sex ratio was 1: 1.1. The successful rate of embryo sexing was increased to the developmental stages.

• Research in Community and Public Health Nursing
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• v.7 no.2
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• pp.257-264
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• 1996

The purpose of this study was to compare health practice and health status college students according to sex. The data consisted of a random sample of 743 students (388 males and 355 females) at 4 colleges in Pohang city. The analytic methods for the study were chi-square analysis and an odds ratio analysis. The results were as follows: 1. The most prevalent religious group was protestant (35.7%). The most prevalent monthly income of parents was 100-200 million won. The educational level of the father was college level or more for 60% of the samples for the mother, 70% was a high school graduate or more. 2. As far as health practice female students practiced better health than male students concerning smoking while male students practiced better health than female students in exercise and BMI(Pdv.001). 3. Odds ratio (OR) of the female students to the male students for BMI (odds ratio=0.30), exercise(odds ratio=0.15), alcohol drinking (odds ratio=O.69) were all significantly smaller than 1. On the other hand, ORS for smoking was significantly greater than 1. 4. A good health pratice score(5-7) were 38.1% in male students and 26.9% in female students. 5. Health pratice had a statistically significant association with physical well-being among male students. OR of the bad health pratice for physical well-being (OR=1.59) was significantly greater than 1.

• Korean Journal of Veterinary Research
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• v.39 no.3
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• pp.478-488
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• 1999

The contents of histidine dipeptides, a metabolic products of muscle protein, were investigated to compare muscle composition among the 9 domestic animals including cattle. In major domestic animals, analyzed the effects of age, part and sex of the animals on their muscle composition. Large amounts of carnosine($68.63{\pm}17.41{\mu}mol/g$) were detected in cattle and it was higher than other animals. Whereas the content of anserine showed high level in order of turkey, chickens and duck. The ratio of carnosine and anserine(C/A ratio) was different depending on the animal species. Statistical data indicated that difference among species was significant(p < 0.05). The contents of histidine dipeptides in heated muscle by boiling for 40 minutes at $110^{\circ}C$ was similar to thoes of raw muscle. C/A ratio in heated muscle was not different from that of raw muscle, indicating that no change has been made after heating process. The contents of carnosine and anserine were different according to the parts of their muscle. Especially chuck of the mammalian showed extremely low level of histidine dipeptides compared with other parts, while C/A ratio maintained certain level regardless of age, part, sex. Therefore, based on the content of histidine dipeptides, could be found the difference of muscle composition among the species. Also C/A ratio of horse, pig, cattle, duck, sheep and turkey were characteristic respectively.

• The Journal of the Korean life insurance medical association
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• v.2 no.1
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• pp.152-181
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• 1985

A statistical analysis on the cardiothoracic ratio in insurance medicine was carried out for 5,200 insured persons who were medically examined including photofluorography of the chest at the Honam medical department, Dong Bang life insurance Company, Ltd from November, 1979 to August, 1984. The results were as follows: 1. The mean value of the cardiothoracic ratio in all of the insured was $44.2{\pm}4.3%$. The mean value of the cardiothoracic ratio was $43.1{\pm}4.1%$ in all males and $45.2{\pm}4.2%$ in all females, and the difference of the values between males and females showed statistical significance(P<0.001). In each age group, the mean value of the cardiothoracic ratios of female was higher than that of male without exception and the difference of the values between males and females showed statistical significance(P<0.001). The mean value of the cardiothoracic ratio showed gradual increase with age from the second to sixth decade in male(P<0.05 or 0.001 after fourth decade) and from the second to seventh decade in female(P<0.05 or 0.001 from the second to sixth decade). 2. Correlation between both sexes and among age groups relating to the cardiothoracic ratios of the insureds seen to be a physiological phenomenon of the cardiac size and should be considered on the rating of the cardiothoracic ratio. Based on the correlation above mentioned and an author's assumption that the incidence of normal and abnormal cardiothoracic ratios in each age group would show the same rate in male and female, author prepared a modified rating table from the existing table; in male group the existing rating table is used and in female group the ratings of 0, 30-50, 50-100 and 100-D are to calculate by the cardiothoracic ratio of 51%or under, 52-56%, 57-61% and 62% or over respectively in the age group below 39, by the cardiothoracic ratio of 52% or under, 53-57%, 58%-62% and 63% or over respectively in the age group of 40-49, by the cardiothoracic ratio of 53% or under, 54-58%, 59-63% and 64% or over respectively in the age group over 60. 3. The relative frequency distribution polygons of the cardiothoracic ratio of both sexes drawn in a pair on one coordinate plane revealed lying in juxtaposition each other horizontally and showed the shifting of females polygon to male's one toward the direction of greater value of the cardiothoracic ratio at a short distance which increased gradually with age. 4. The minimum cardiothoracic ratio was 31.2% and the maximum cardiothoracic ratio was 63.6% in all of the insured. 5. In each age group, no significant sex difference was found in the relative frequency distribution of ratings by the cardiothoracic ratios of 5,200 insureds by using the rating table modified by author, while significant sex difference was found by using the existing rating table.