• Title/Summary/Keyword: Service Laboratory

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Seasonal survey on the respiratory diseases of slaughtered pigs in Jeonbuk, Korea (전북지역 도축돈 호흡기질병 조사)

  • Lim, Mi-Na;Kim, Chul-Min;Park, Young-Min;Song, Ju-Tae;Jin, Jae-Kwon;Cho, Hyun-Ung
    • Korean Journal of Veterinary Service
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    • v.39 no.4
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    • pp.231-237
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    • 2016
  • The present study was conducted to investigate the lesion of red internal organs in slaughtered pigs and provided assistant data for pig farms. During March to December 2015, a total of 1,160 lung samples out of 58 herds were collected randomly from pigs slaughtered in Jeonbuk province. In addition, 290 hilar lymph nodes from pig with pneumonic lung lesion (5 samples per herd) were screened for selected viral and bacterial pathogens. Gross lesions of lungs such as swine enzootic pneumonia (SEP), pleuritis, pleuropneumonia, pericarditis and liver white spots were examined. The overall prevalence of SEP was 64.3% (746/1,160). In the analysis of seasonal prevalence, there was an increase of occurrence during the spring months (287/400, 71.8%) and decrease during the fall months (93/200, 46.5%) among the whole herds. The mean number of SEP score per pig was $1.20{\pm}1.28$. The prevalence of pleuropneumonia, pleuritis, pericarditis, and milk spot was 25.5% (296/1,160), 44.1% (512/1,160), 3.8% (44/1,160) and 17.6% (204/1,160), respectively. The most frequent region with lung lesion was diaphragmatic lobes (left 17.1%, right 17.3%). In the detection of viral pathogens by PCR, porcine circovirus type2 (PCV2) was positive in 86.9% (252/290), while porcine reproductive and respiratory syndrome virus (PRRSV) was not detected, In the case of bacterial pathogens, 50 microorganisms were isolated by PCR and/or microbiological test. The most frequently isolated bacteria was Streptococcus suis (20, 34.4%), followed by Pasteurella multocida (17, 29.3%), Streptococcus spp. (11, 3.4%), Actinobacillus pleuropneumoniae (2, 8.9%).

Prevalence of Coxiella burnetii in native Korean goat in Jeonbuk province (전북지역 염소에서 Coxiella burnetii 감염실태 조사)

  • Gang, Su-Jin;Jeong, Jae-Myong;Kim, Hyun-Kwan;Lee, Jae-Wook;Shon, Ku-rye;Park, Tae-Wook
    • Korean Journal of Veterinary Service
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    • v.39 no.4
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    • pp.239-246
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    • 2016
  • The prevalence of Coxiella burnetii in native Korean goats in Jeonbuk province was investigated using ELISA (sera) and PCR (vaginal mucus). A total of 798 blood samples from 189 farms were collected in 2013 and 2015. Seroprevalence was 13.6% in 2013 and 15.7% in 2015. Tracking survey of six seropositive farms on the prevalence of C. burnetii was conducted. 137 (41.4%) out of 331 goats were seropositive and 47 (17.6%) out of 267 goats were positive in PCR. The higher seropositivity observed in adult goats and female goats significantly, the seroprevalence of C. burnetii was higher as age increased (<1 y=13.2%~${\geq}5$ y=100%)(P<0.05) and female goats (44.8%) was higher than male goats (24.1%) on the seroprevalence (P<0.05). 21 (7.9%) goats positive in PCR was seronegative. The prevalence of C. burnetii in native Korean goats in slaughter house was 30.0% in ELISA and 11.5% in PCR. Significantly, female goats (62.8%) was higher than male goats (12.3%) on the seroprevalence (P<0.05). Based on these data, C. burnetii was easily exposed in domestic animals: native Korean goats-related areas such as farms and slaughter house.

Prevalence study of bovine viral diarrhea virus (BVDV) from cattle farms in Gyeongsangnam-do, South Korea in 2021 (2021년 경남지역 소바이러스성설사 바이러스(BVDV) 감염실태 조사)

  • Son, Yongwoo;Cho, Seonghee;Ji, Jeong-Min;Cho, Jae-Kyu;Bang, Sang-Young;Choi, Yu-Jeong;Kim, Cheol-Ho;Kim, Woo Hyun
    • Korean Journal of Veterinary Service
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    • v.45 no.3
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    • pp.211-219
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    • 2022
  • Bovine viral diarrhea (BVD) is one of the problematic wasting diseases in cattle leading to huge economic losses. This study was conducted to investigate the prevalence of BVD including transient and persistent infection from cattle farms in Gyeongsangnam-do. A total of 2,667 blood samples from 24 farms were collected and the sera were subjected to ELISA to detect BVD virus (BVDV) antigen, Erns. 5' untranslated region (5'-UTR) of BVDV-positive samples was sequenced to identify the genotype, and compared with isolates previously reported elsewhere. There were fourteen BVDV-positive calves from 2,667 samples (positive rate: 0.52%) from first ELISA testing followed by eight persistently infected out of eleven BVDV-positive samples (72.73%) in secondary ELISA that was conducted in at least four weeks suggesting the circulation of BVDV in the area. Sequencing analysis exhibited that thirteen BVDV-positive samples were identified as BVDV-1b and one sample was BVDV-2a. Phylogenetic analysis revealed that the BVDV-1b-positive samples showed the highest homology in nucleotide sequence to Korean isolates collected from Sancheong, Gyeongsangnam-do, while the BVDV-2a-positive sample (21GN7) was more similar to reference strains collected outside South Korea. This study will provide the recent fundamental data on BVD prevalence in Gyeongsangnam-do to be referred in developing strategies to prevent BVDV in South Korea.

Isolation of Staphylococcus aureus and detection of enterotoxin from pigs and cattle carcass by PCR (소와 돼지 도체표면에서 황색포도상구균의 분리 및 장독소 검출)

  • Lee, Woo-Won;Jung, Byeong-Yeal;Kim, Sang-Hyun;Lee, Seung-Mi;Lee, Gang-Rok;Kim, Geum-Hyang;Kim, Yong-Hwan
    • Korean Journal of Veterinary Service
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    • v.33 no.3
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    • pp.255-261
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    • 2010
  • At the present study, it was aimed to explore the states of antimicrobial resistant Staphylococcus aureus isolates from 320 pigs and cattle carcass (160 pigs and 160 cattle) slaughtered in Busan province from March 2008 to November 2009. Among 320 samples, 26 of Staphylococcus aureus were isolated from pigs (10.6%) and cattle (5.6%). In antimicrobial susceptibility test, all of the isolates were demonstrated susceptibility to oxacillin, cefoxitin, cephalothin, vancomycin, rifampin and linezolid. But the isolates were showed resistance other antibiotics in order of penicillin (92.3%), gentamicin (76.9%), tetracycline (69.2%), erythromycin (65.4%), and clindamycin (61.5%). In case of enterotoxin production, 7.7% of 2 strains produced enterotoxin A.

Study on antimicrobial resistance of staphylococcus aureus isolated from bovine mastitic milk and several animals in kyongbuk northern province and detection of MRSA from the isolates of S aureus (경북북부지역 젖소 유방염 우유 및 각종 동물로부터 분리한 staphylococcus aureus의 항균제 내성과 MRSA검출에 관한 연구)

  • Kim, Sin;Oh, Yu-Mi;Kim, Sang-Yun;Woo, Young-Ku;Gwon, Heon-Il
    • Korean Journal of Veterinary Service
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    • v.23 no.2
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    • pp.153-163
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    • 2000
  • This study was carried out the antimicrobial resistance of staphylococcus aureus isolated from bovine mastitic milk, chickens, korean native cattle, korean native goats, pigs, dog and mice in northern area of kyongbuk. The result were summarised as follows ; A total of 149 S aureus were isolated from bovine mastitic milk, chickens, korean native cattle, korean native goats, pigs, dog and mice. In 80 isolates of S aureus from bovine mastitic milk, 60% of isolates revealed resistance to penicillin and ampicillin, 19% to gentamicin, 6% to tetracycline. In 36 isolates of S aureus from chickens, 72% of isolates revealed resistance to tetracycline, 58% to penicillin and ampicillin, 42% to streptomycin, 31% to lincomycin, 25% to norfloxacin, 22% to gentamicin. In 17 isolates of S aureus from korean native cattle, 100% of isolates revealed resistance to penicillin and ampicillin, 88% to lincomycin, 76% to tetracycline. 2 MRSA were isolated from the isolates of S aureus from bovine mastitic milk and revealed multi-drug resistance.

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Comparison of detecting methods and the relationship between tissue and blood for enrofloxacin and metabolic ciprofloxacin residues in broiler chickens (육계에서 조직과 혈액의 enrofloxacin 및 대사성 ciprofloxacin 잔류 상관관계 조사 및 검사방법간의 비교)

  • Sung, Myung-Suk;Kim, Mi-Suk;Seo, Hee-Jin;Bae, Dong-Rok;Hwang, Ji-Young;Kim, Soon-Tae;Cho, Jong-Suk;Park, Hong-Je;Hong, Sung-Hee;Kim, Gyung-Dong;Jang, Seong-Jun;Yun, Mun-Jo
    • Korean Journal of Veterinary Service
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    • v.36 no.4
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    • pp.311-320
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    • 2013
  • The purpose of this study was to evaluate detecting methods and the relationship between tissues and blood for enrofloxacin and metabolic ciprofloxacin residues in broiler chickens. Two groups of broiler chickens were administrated via the drinking water with $50{\mu}g/mL$ and $100{\mu}g/mL$ of enrofloxacin for 5 days, respectively. The concentration of enrofloxacin and metabolic ciprofloxacin in tissues (muscle and kidney) and blood were measured during administration period (for 5 days) and withdrawal period (for 12 days) by high performance liquid chromatography (HPLC) method. Also, all samples were conducted for screening of residues by microbial method using E. coli for quinolone detection and immuno-chromatography method using Smart kit. The relationship between tissues (muscle and kidney) and blood for enrofloxacin and metabolic ciprofloxacin residues in broiler chickens was followed : The levels of enrofloxacin and metabolic ciprofloxacin residues in muscle and kidney were higher 2.9~3.2 folds, 3.6~3.8 folds more than the residues levels in blood, respectively. These results support we can predict the residues in muscle and kidney from the residues in blood. In comparison of detecting methods for antibiotic residues, microbial method using E. coli for quinolone detection and immuno-chromatography method using Smart kit could detect positive reaction at similar or lower concentration than violative concentration of enrofloxacin and metabolic ciprofloxacin in chicken tissues. These results support what two screening methods are useful for screening of quinolone detection in chickens.

5GCHAMPION - Disruptive 5G Technologies for Roll-Out in 2018

  • Strinati, Emilio Calvanese;Mueck, Markus;Clemente, Antonio;Kim, Junhyeong;Noh, Gosan;Chung, Heesang;Kim, Ilgyu;Choi, Taesang;Kim, Yeongjin;Chung, Hyun Kyu;Destino, Giuseppe;Parssinen, Aarno;Chuberre, Nicolas;Vautherin, Benoit;Deleu, Thibault;Gineste, Mathieu;Korvala, Aki
    • ETRI Journal
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    • v.40 no.1
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    • pp.10-25
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    • 2018
  • The 5GCHAMPION Europe-Korea collaborative project provides the first fully-integrated and operational 5G prototype in 2018, in conjunction with the 2018 PyeongChang Winter Olympic Games. The corresponding technological advances comprise both an evolution and optimization of existing technological solutions and disruptive new features, which substantially outpace previous generations of technology. In this article, we focus on a subset of three disruptive technological solutions developed and experimented on by 5GCHAMPION during the 2018 PyeongChang Olympic Games: high speed communications, direct satellite-user equipment communications, and post-sale evolution of wireless equipment through software reconfiguration. Evaluating effectiveness and performing trials for these key 5G features permit us to learn about the actual maturity of 5G technology prototyping and the potential of new 5G services for vertical markets and end user enhanced experience two years before the launch of large-scale 5G services.

Energy-efficient Relay MAC with Dynamic Power Control in Wireless Body Area Networks

  • Cai, Xuelian;Yuan, Jingjing;Yuan, Xiaoming;Zhu, Wu;Li, Jiandong;Li, Changle;Ullah, Sana
    • KSII Transactions on Internet and Information Systems (TIIS)
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    • v.7 no.7
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    • pp.1547-1568
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    • 2013
  • Wireless body area network (WBAN) is an emerging short-range wireless communication network with sensor nodes located on, in or around the human body for healthcare, entertainment and ubiquitous computing. In WBANs, energy is severely constrained which is the prime consideration in the medium access control (MAC) protocol design. In this paper, we propose a novel MAC protocol named Energy-efficient Relay MAC with dynamic Power Control (ERPC-MAC) to save energy consumption. Without relying on the additional devices, ERPC-MAC employs relaying nodes to provide relay service for nodes which consume energy fast. Accordingly the superframe adjustment is performed and then the network topology can be smoothly switched from single-hop to multi-hop. Moreover, for further energy saving and reliability improvement, the dynamic power control is introduced to adjust the power level whenever a node transmits its packets to the coordinator or the relaying node. To the best of the authors' knowledge, this is the first effort to integrate relay, topology adjustment and power control to improve the network performance in a WBAN. Comprehensive simulations are conducted to evaluate the performance. The results show that the ERPC-MAC is more superior to the existing standard and significantly prolongs the network lifetime.

Research on a Mobile-aware Service Model in the Internet of Things

  • An, Jian;Gui, Xiao-Lin;Yang, Jian-Wei;Zhang, Wen-Dong;Jiang, Jin-Hua
    • KSII Transactions on Internet and Information Systems (TIIS)
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    • v.7 no.5
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    • pp.1146-1165
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    • 2013
  • Collaborative awareness between persons with various smart multimedia devices is a new trend in the Internet of Things (IoT). Because of the mobility, randomness, and complexity of persons, it is difficult to achieve complete data awareness and data transmission in IoT. Therefore, research must be conducted on mobile-aware service models. In this work, we first discuss and quantify the social relationships of mobile nodes from multiple perspectives based on a summary of social characteristics. We then define various decision factors (DFs). Next, we construct a directed and weighted community by analyzing the activity patterns of mobile nodes. Finally, a mobile-aware service routing algorithm (MSRA) is proposed to determine appropriate service nodes through a trusted chain and optimal path tree. The simulation results indicate that the model has superior dynamic adaptability and service discovery efficiency compared to the existing models. The mobile-aware service model could be used to improve date acquisition techniques and the quality of mobile-aware service in the IoT.

Recent Progress in Understanding Host Mucosal Response to Avian Coccidiosis and Development of Alternative Strategies to Mitigate the Use of Antibiotics in Poultry Production

  • Lillehoj, Hyun-Soon;Lee, Sung-Hyen;Jang, Seung-Ik;Kim, Duk-Kyung;Lee, Kyung-Woo
    • Korean Journal of Poultry Science
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    • v.38 no.4
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    • pp.275-284
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    • 2011
  • As the world population grows and developing countries become more affluent, the global consumption of meat will increase by more than 50% within the next 10 years. Confronting the increased demand for poultry food products are emerging field diseases, increasing regulatory bans of antimicrobial growth promoters, high-density growth conditions, and waste management. Although biotechnology offers solutions to some of these challenges, basic studies are needed to better understand the complex interaction between the intestinal microbiome, host immunity and the environment. This presentation will focus on emerging strategies to enhance gut immunity and to decrease economic losses due to poultry diseases. This presentation will highlight recent developments in coccidiosis research and provide information on host immunity, immunomodulation, and the latest advances in dietary and nutritional approaches against coccidiosis. Such information will magnify our understanding of host-parasite biology, mucosal immunology, and design of future nutritional interventions and vaccination strategies for coccidiosis.