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경북지방 개 브루셀라병 다두 발생농장의 혈청학적 및 세균학적 조사 (Serological and bacteriological study on canine brucellosis in the large kennel farms in Gyeongbuk province)

  • 김성국;서희진;김순태;장영술;조민희
    • 한국동물위생학회지
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    • 제33권2호
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    • pp.129-134
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    • 2010
  • Canine brucellosis is a contagious disease of the reproductive tract that cause mainly abortion and infertility in dog. A serological and bacteriological survey was conducted for breeding kennels which were suffered from frequent outbreak of canine brucellosis in Gyeongbuk province in 2009. Among 138 samples, 45 serum samples were sero-positive. Brucella canis was isolated from 30 blood samples of the seropositive cases, and from 2 samples of 62 sero-negatives. The biochemical properties of 32 isolates were characterized with no production of H2S, no fermentation of carbohydrates, hydrolyzation of urea, and development of thionin dye medium. At amplification of BCSP and 16S-rRNA gene using PCR, 711bp and 905bp DNA fragments were detected in agarose. Three tandem repeat pattern was shown in genotyping by Multi-locus VNTR assay (MLVA).

Bovine leukosis in a slaughtered cow

  • Lee, Sang-Myung;Choi, Hae-Yeun;Kwak, Hak-Koo;Jung, Chan-Woo;Han, Seong-Tae;Park, Sun-Jun;Yoon, Ho-Gyu;Jang, Rae-Hun
    • 한국동물위생학회지
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    • 제30권4호
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    • pp.551-555
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    • 2007
  • A slaughtered 3 years old cow was diagnosed as bovine leukosis by postmortem inspection, agar gel immunodifusion (AGID) test, and polymerase chain reaction (PCR). It showed no special clinical signs except emaciation. Grossly, we observed enlargement of mediastinal and pelvic lymph nodes, and tubercle -like mass on pleura and lung surface, and masses on right atrium and ventricle.

Coinfected cases with equine herpesvirus type 1, 4 and Streptococcus equi subsp. zooepidemicus in throughbred horse

  • Kim, Seong-Guk;Cho, Gil-Jae;Cho, Min-Hee;Kim, Young-Hoan;Lee, Hong-Young;Choi, Jeong-Hye;Kim, Jeong-Hwa;Choi, Seong-Kyoon
    • 한국동물위생학회지
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    • 제34권2호
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    • pp.187-190
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    • 2011
  • The Thoroughbred horse was an approximately 4-years-old castrated male with highly emaciation, nasal epistaxis and subsequently died. Gross necropsy revealed epistaxis and hyperemia on the lung, multiple hemorrhage in muscle, and liver was focally attached to the peritoneum with fibrin. According to polymerase chain reaction (PCR), Equine herpes virus type 1 and 4 (EHV type 1, 4) was detected in the lung and trachea. In bacterial culture from kidney, liver, spleen, muscle and blood, Streptococcus equi subsp. zooepidemicus was isolated. Based on the gross lesion and PCR, this horse was diagnosed as EHV type 1, 4 and S. zooepidemicus coinfection.

음성정보기술을 이용한 통신서비스 - KT 서비스를 중심으로 - (Telecommunication Services Based On Spoken Language Information Technology - In view of services provided by KT -)

  • 구명완;김재인;정영준;김문식;김원우;김학훈;박성준;류창선;김희경
    • 대한음성학회:학술대회논문집
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    • 대한음성학회 2004년도 춘계 학술대회 발표논문집
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    • pp.125-130
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    • 2004
  • In this paper, we explain telecommunication services based on spoken language information technology. There are three different kinds of services. The first is based on Advanced Intelligent services(AIN). We built a Intelligent Peripheral(IP)with speech recognition, speech synthesis and VoiceXML interpreter. The second is based on KT-HUVOIS, a proprietary speech platform based on VoiceXML. The third is based on VoiceXML interpreter. We explain various services depending on these platforms in detail.

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영천지역 구제역 바이러스 구조단백질 항체가 조사 (Survey of foot-and-mouth disease virus structural protein antibody titer in Yeongcheon)

  • 손준형;황유선;손규희;신성호;이은미;김순태;조민희;윤문조
    • 한국동물위생학회지
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    • 제38권1호
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    • pp.13-17
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    • 2015
  • Three serotypes (O, A and Asia1) of the foot-and-mouth disease (FMD) vaccine were injected into domestic cloven-hoofed animals in korea after the nationwide spread at the end of 2010. The purpose of this study was survey of FMD virus stuructural protein (SP) antibody titer in Yeongcheon by enzyme-linked immunosorbent assay (ELISA). Total 1,324 samples collected from 89 farms were tested. The overall seroprevalence of FMD virus SP antibodies was 58.8% (778/1,324) The seroprevalence of FMD virus SP antibody varied with species. Results in cattle (over 12 month old) and pig (90 to 130 day old) were 58.8% and 44.9% respectively.

Brucella canis 감염견에 대한 항균제 치료 (Two cases of antibiotic therapy in dog infected with Brucella canis)

  • 김성국;김영환;박인화;장성준;조광현;이양수
    • 한국동물위생학회지
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    • 제29권1호
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    • pp.47-53
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    • 2006
  • For examination of antibiotic therapeutic efficacy in canine brucellosis, this examination was carried out two female bitches infected with Brucella canis in Gyeongbuk province, and used combicillin, baytril and doxycycline in susceptible antibiotics at B canis. During 18 month after the termination of antibiotic therapy, blood sample of the two bitches were examined for B canis antibody and antigen. The antibody of one bitch was disappeared at 5 month after antibiotic therapy and the other was continued at 18 month, but two bitches were not detected antigen by blood culture and PCR. Examination of blood chemical value (AST, ALT, urea, creatinine) of two bitches was increased in AST value during antibiotic therapy.

경북지역 양돈장의 돼지생식기호흡기증후군(PRRS) 항체가 조사 (Seroprevalence of porcine reproductive and respiratory syndrome (PRRS) in Gyeongbuk province)

  • 손준형;신성호;이은미;김순태;조민희;윤문조
    • 한국동물위생학회지
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    • 제38권1호
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    • pp.9-12
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    • 2015
  • The purpose of this study was survey of porcine reproductive and respiratory syndrome (PRRS) virus antibody in Gyeongbuk province by enzyme-linked immunosorbent assay (ELISA). Total 690 samples collected from 15 pig farms were tested. The overall seroprevalence of PRRS virus antibodies was 63.2% (436/690) and 13 farms of 15 farms had at least one pig with PRRS virus antibodies. The seroprevalence of PRRS virus antibody varied with age. Results in 1 to 30 day old, 31 to 60 day old, 61 to 90 day old, 90 to 120 day old and over 120 day old pig were 58.3%, 36.0%, 68.0%, 84.0%, 80.0% and sow were 61.9% respectively.

Brucellosis outbreak of Korean indigenous cattle at Yeongwol and Pyeongchang county in Korea

  • Kim Song-Tae;Yoon Kyo-Bok;Kang Tae-Kyu;Bak Won-Hern;Lee Jung-Hyun;Chung Dong-Su
    • 한국동물위생학회지
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    • 제28권4호
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    • pp.387-392
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    • 2005
  • This study was attempted to investigate the properties of brucellosis in Korean indigenous cattle at the Yeongwol and Pyeongchang county. Brucella spp was differentiated and identified from cotyledons, amniotic fluids and supramammary lymph nodes which confirmed with clinical, serological, epidemiological evidences (69 cases) from January to June, 2004. Isolation frequency of this causative agent from supramammary lymph nodes, cotyledons and amniotic fluids from 38 pregnant Korean indigenous cattle were $39.1\%,\;87.5\%,\;and\;63.2\%$, respectively, and finally confirmed with Brucella abortus biotype 1 through biochemical and serological test. A Brucella specific DNA with 711bp band was detected by PCR assay using BCSP primer. The two cases were definite epidemiological evidences that infected Korean indigenous cattle acrossed the border to Yeongwol and Pyeongchang from near two provinces. Effective prevention programs are urgently needed for further spreading this epidemics.

PFGE를 이용한 경북지역에서 분리된 Brucella abortus의 유전형별 (Genotyping of Brucella abortus isolated in Gyeongbuk province by PFGE)

  • 조민희;김성국;김영환;김순태;엄현정;장영술;고영활
    • 한국동물위생학회지
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    • 제32권3호
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    • pp.257-264
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    • 2009
  • Subtyping of Brucella abortus isolates is epidemiologically important for monitoring of bovine brucellosis outbreaks. Pulsed-field gel electrophoresis (PFGE) is considered as a gold standard of molecular typing methods to study the DNA polymorphisms of bacteria. In this study, we analyzed using PFGE the DNA fragment profiles of B. abortus isolated in Gyeongbuk province from 1998 to 2006. The genomic DNA was digested with the restriction endonuclease Xba I, Xho I and Smi I followed gel electrophoresis. No distinguishable patterns of the genomic DNA digested with Xba I and Xho I were observed among the field isolates of B. abortus tested in this study. But Smi I restriction enzyme resulted in two PFGE patterns consisting of 13-15 bands that ranged in size from 33 to 668bp by standard marker. The cluster analysis by DNA fingerprinting software showed 93.75% similarity between two PFGE patterns. No different PFGE patterns were recognized among the isolates originated from various years, regions and cow breeds.

경북지역 돼지 유래 Pasteurella multocida 의 특성 및 항생제 내성양상 (Characteristics and antimicrobial resistance patterns of Pasteurella multocida isolated from swine in Gyeongbuk province)

  • 손준형;김영환;신성호;이은미;김순태;조민희;윤문조
    • 한국동물위생학회지
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    • 제37권3호
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    • pp.165-171
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    • 2014
  • This study was conducted to investigate the species-specific gene detection, capsular serogroup and antimicrobial resistance pattern of Pasteurella multocida isolated from pneumonic lung lesion of swine in Gyeongbuk province. P. multocida isolates were typed for capsular serogroups by polymerase chain reaction. Of the 32 strains, 28 (87.5%) were typed serotype A, 3 (9.3%) were typed serotype D, and 1 strain was unknown (3.1%), respectively. In antimicrobial agents resistance test, almost of strains were susceptible to amoxicillin (100%), enrofloxacin (96.9%), ampicillin (93.8%), florfenicol (90.6%), chloramphenicol (90.6%) and were resistant to streptomycin (71.9%), spectinomycin (56.3%). All strains were resistant to clindamycin, erythromycin and lincomycin.