• Title/Summary/Keyword: Serum samples

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INCIDENCE OF SHORT OESTRUS CYCLES IN NILI-RAVI BUFFALOES

  • Chohan, K.R.;Chaudhry, R.A.;Awan, M.A.;Naz, N.A.
    • Asian-Australasian Journal of Animal Sciences
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    • v.5 no.3
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    • pp.583-585
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    • 1992
  • A study was undertaken to determine the incidence of short oestrus cycles and associated serum progesterone levels in Nili-Ravi buffaloes. Forty lactating buffaloes were kept under observation for oestrus activity for a period of sixty days. Blood samples from jugular vein from twenty buffaloes were used to determine associated serum progesterone levels during short oestrus cycles. Incidence of short oestrus cycles was 15.5 percent (Nine out of fifty eight oestrus cycles) and most of the short oestrus cycles occurred between first and second oestrus after calving. Lengths of short oestrus cycles ranged from 6 to 14 days. The most frequent length was 11 days. Serum progesterone levels were lower at oestrus and increased gradually attaining peak levels of 1.3 and 1.8 ng/ml on 7th day of 9 and 11 day short oestrus cycles. One buffalos exhibiting a short oestrus cycle of 12 days conceived which indicated that ovulation took place at the oestrus of short cycle.

Studies on the Change of Serum LH, FSH and Prolactin Levels in the Puerperal Sow (돼지에 있어서 분만전후의 혈청중 LH, FSH 및 Prolactin 수준의 변화에 관한 연구)

  • 정영채;김창근;이규승;박창식;백무용
    • Korean Journal of Animal Reproduction
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    • v.5 no.1
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    • pp.31-35
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    • 1981
  • The Purpose of this study was to determine the serum content of LH, FSH and prolactin. Blood samples collected from day 20 prepartum to 20 postrtum in 8 sows. LH, FSH and prolactin were assayed by radioimmunoassay methods. LH levels increased from 2.3 mIU/$m\ell$ day 6 prepartum to 5.8 mIU/$m\ell$ by day +2 and remained quite constant thereafter. The mean serum FSH increased from 7.5mIU/$m\ell$ day 6 prepartum to 10.0 mIU/$m\ell$ at the time of parturition, but the difference in LH and FSH patterns reported here provide further evidence that the controlling mechanisms for the two gonadotropins are independent. Prolactin reached a peak mean level of 68.5ng/$m\ell$ at day 0.

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Pregnancy Diagnosis of Hanwoo(Korean Native Cattle) by Serum Progesterone Concentration during Early Gestaton (한우에 있어서 혈청 Progesterone 농도 측정에 의한 조기임신진단)

  • 백광수;성환후;오성종;양보석;이명식;정진관
    • Korean Journal of Animal Reproduction
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    • v.19 no.3
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    • pp.197-203
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    • 1995
  • Serum progesterone concentrations of pregnant and non-pregnant Hanwoo were compared to evaluate the possibility of early pregnancy prediction. Twenty five female Hanwoo were devided into two groups. Eighteen heads were injected with 980$\mu\textrm{g}$ tiaprost trometamol salt(Iliren:PGF2$\alpha$ analogue) for estrous synchronization and fifteen heads of them were inseminated artifically and seven heads were non-treated. Blood samples were collected from the jugular vein before PGF2$\alpha$ injection and on day 0, 18, 21 after estrous induction. Pregnancy of cattle was confirmed by rectal palpaton after 60 days of artificial insemination. The serum progesterone concentrations at heat showed no significant differences between pregnant and non-pregnant while on day 18 and 21 after estrous induction showed significant differences(p<0.05). The accuracy of pregnancy diagnosis on day 18 and 21 after estrous induction was 85.7 and 71.4% respectively while that of non-pregnancy were 71.4 and 100.0% respectively.

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Serum IgE Immune Response on the Exposure of Korean Ginseng

  • 이종화;박경미;박화진
    • Proceedings of the Korean Society of Applied Pharmacology
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    • 1994.04a
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    • pp.315-315
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    • 1994
  • Recently, it was reported that Pfaffia paniculata, known as Brazil ginseng, caused occupational asthma. To assess a potential relationship between Korean ginseng and allergic reactions, total IgE in serum, allergologic parameter, was investigated. Serum total IgE were measured in 8 takers of ginseng for more than 5 yr, 4 subjects of weak constitution, 10 workers participated in the washing of fresh ginseng in factory and 7 control subjects, Blood samples were taken before and after the scheduled exposure to Korean ginseng, sera were kept frozen until assay. Total IgE levels after the scheduled exposure to Korean ginseng were not significantly different in each group and total IgE levels were not significantly different in the control subjects compared with those of other groups at the start of experiment and at the end of experiment. These results suggest that the exposure to Korean ginseng dose not affect significantly the IgE immune response in volunteers. No changes of levels of IgE on the exposure of Korean ginseng may represent additional evidence of the lack of antigenic potential of Korean ginseng.

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Relationship of Habitual Alcohol Consumption to the Nutritional Status in Middle Aged Men (음주습관이 중상류층 중년남성의 영양상태에 미치는 영향)

  • 이선희;김화영
    • Journal of Nutrition and Health
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    • v.24 no.1
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    • pp.58-65
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    • 1991
  • The effect of habitual alcohol consumption on the nutrient intake. body weight and serum values were studied in 194 upper middle class men aged 35-55yr. living in Seoul. Each subject was interviewed to get information of alcohol and nutrients intake. Blood samples were taken. Mean values for each variable were compared by fertile of alcohol intake. The average alcohol consumption of the subject was 32.2g/day. Most of times foods were taken along with drinks. The subjects preferred food items such as meat. fish, nuts. dry fishes when they drink. High alcohol consumers had a significantly higher intake of calorie. protein, fat. Fe, and riboflavin compared to nondrinkers and moderate drinkers. Body weight was significantly higher in high alcohol drinkers followed by moderate and nondrinker groups. They also showed the higher serum values of triglyceride and r-GTP. High alcohol consumption resulted in an increased intakes of most nutrients especially calorie and fat which leads to higher body weight and higher serum concentrations of triglyceride and r-GTP. Therefore it seems that heavy alcohol drinkers are more prone to obesity and related chronic diseases.

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Serum IgE Immune Response After the Exposure to Korean Ginseng (고려인삼이 혈청 면역글로부린 E 면역반응에 미치는 영향)

  • Lee, Jong-Wha;Park, Kyeong-Mee;Park, Ki-Hyun
    • Journal of Ginseng Research
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    • v.18 no.2
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    • pp.113-117
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    • 1994
  • To assess a polential relationship of Korean ginseng to allergic reactions, the effect of Korean ginseng on the total IgE in serum as an allergologic parameter was investigated in humans. Serum total IgE levels were measured in 8 subjects who have been taking ginseng for more than 5 years, 4 subjects of weak constitution, 10 newly hired workers in the ginseng processing industry and 7 normal subjects unexposed to Korean ginseng as control group. Blood samples were taken before and after the exposure to Korean ginseng. Total IgE levels after the exposure to Korean ginseng were not significantly different from those before the exposure within each group. And also, the IgE levels of control subjects were not significantly different compared with those of other groups. These results suggest that the exposure to Korean ginseng dose not affect significantly the IgE immune response of the subjects.

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Ultra-sensitive Determination of Salinomycin in Serum Using ICP-MS with Nanoparticles

  • Cho, H.K.;Lim, H.B.
    • Bulletin of the Korean Chemical Society
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    • v.35 no.11
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    • pp.3195-3198
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    • 2014
  • An ultra-sensitive detection method for small molecules such as antibiotics was developed using ICP-MS with magnetic and $TiO_2$ nanoparticles. Since most of the antibiotics are too small to employ a sandwich-type extraction through an immunoreaction, a non-specific platform was employed, in which the target was extracted by magnetic separation, followed by tagging with $TiO_2$ nanoparticles of 11.2 nm for ICP-MS measurement. The detection limit for salinomycin obtained from spiked serum samples was $0.4ag\;mL^{-1}$ (${\pm}10.3%$), which was about $1.5{\times}10^6$ times lower than that of LC-MS/MS and about $1.2{\times}10^{11}$ times better than that of ELISA. Such an excellent sensitivity enabled us to study the toxicity of antibiotics exposed to human beings by determining them in serum.

The Effect of Different dietary Patterns (between Non-Vegetarian and Vegetarian) on Protein Nutritional Status during Short-term (식사 Pattern의 차이가 단백질 영양상태에 미치는 영향)

  • 박귀선;김정임
    • Journal of the East Asian Society of Dietary Life
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    • v.7 no.1
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    • pp.1-11
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    • 1997
  • The purpose of this study was to describe the protein nutritional status of female collegians between nonvegetarian diet groups(14) and vegetarian diet groups(19). Daily intake, protein were calculated from food direct measurement. Urea/creatinine, muscle mass, fatique conditions and hematological parameter were calculated. Blood samples were analyzed for total protein, albumin ammonia, urea, uric acid, creatinine, BUN contents. The results obtained are summarized as following : 1) On total dietary intake, especially Fe, vitamin C, niacin intake, vegetarian groups were higher than non vegetarian groups. 2) On composition of EAA(essential amino acids), vegetarians were highter than non vegetarian of leucine contents, but were lower lysine and threonine contents. 3) On urea / creatinine excretion, vegetarians were higher than non begetarians. Muscle mass were non signicant, fatique condition, vegetarians were normal state. 4) Non vegetarians were increased serum albumin total protein, uric acid, ammonia but vegetarians were decreased BUN, ammonia after experimental diet intake. 5) In process of time after meal, non vegetarians were increased serum albumin total protein, BUN, but vegetarians were decreased ammonia.

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A Study of Calcium Status and Effect of Nutrition Education of Prevention Osteoporosis in Middle - Women (장년기 여성의 칼슘영양상태와 골다공증 예방을 위한 영양교육의 효과)

  • Hong, Sun-Myeong;Kim, Hyeon-Ju
    • Journal of the Korean Dietetic Association
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    • v.7 no.2
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    • pp.159-166
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    • 2001
  • This study was conducted to investigate the relationship of calcium intake , serum calcium level to calcium supplementation and the effect of nutritional education in middle-aged women. The age distribution of the subjects was 49-55 years. Daily nutrient intakes and dietary sources of calcium were analyzed by convenient method and food frequency questionnaire. At the same time, fasting blood samples were collected and serum calcium and magnesium concentrations were measured before and after nutritional education. The BMD of subjects were measured by Dual Energy X-ray Absorptiometry(DEXA). Serum calcium and magnesium concentrations were significantly(p<0.05) different between pre and post nutritional education. After nutritional education, nutrient intakes were significantly increased(p<0.05), especially calcium. This results suggested that dietary calcium intake as well as nutritional education seem to be important in preventing in middle-aged women.

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Studies on the Concentrations of Cd, Pb, Hg and Cr in Dog Serum in Korea

  • Park, S.H.;Lee, M.H.;Kim, S.K.
    • Asian-Australasian Journal of Animal Sciences
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    • v.18 no.11
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    • pp.1623-1627
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    • 2005
  • Heavy metal pollution has become a serious health concern in recent years. Dogs are a very good indicator of the pollution load on the environment. They share people's environment and are exposed to the action of the same pollutants. This study estimated the heavy metal contents in the serum of dogs in domestic districts, and assessed the age, sex, feeding habits, living area, breeding environment and smoking habit of the owners. The findings suggest that dogs can be used to monitor the environmental quality of heavy metals. The mean concentrations of heavy metals in the dog serum from 204 samples (108 male and 96 female) were 0.22${\pm}$0.01 $\mu$g/ml, 0.24${\pm}$0.04 $\mu$g/ml, 0.61${\pm}$0.08 $\mu$g/ml, and 0.50${\pm}$0.06 $\mu$g/ml (for Cd, Hg, Pb, and Cr), respectively. Concentrations of Pb, Cd, Hg, and Cr in the dog serum were higher in Yeongnam including Ulsan, and Seoul higher than those of Chungchong and Honam, especially Pb concentration, which was significantly higher (p<0.01). Concentrations of Cd, Hg, Pb, and Cr in serum, were increased by age (p<0.05). When commercial pet food was provided to dogs, Cd and Cr concentrations were significantly higher in dog serum than dogs fed a human diet (p<0.01 in Cd and p<0.05 in Cr). Heavy metal concentrations of dogs owned by smoking owners, were higher than non-smoking owners although there was no significant difference.