• KSBB Journal
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• 제11권1호
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• pp.92-98
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• 1996

전남 법성포 해안의 갯벌 시료로부터 chitinase 생 생 균주를 분리하였으며, 분리된 균주 중에서 chiti­n nase 생성능이 우수한 JM을 선발 동정하여 Serranasetia sp. JM으로 명명하였다. Serratia sp. JM은 nu­trient 배지냐 MacConkey 배지에서 prodigiosin 색소를 생성하며, 정제 chitin이 포함된 한천 배지에서 는 chitinase 생성에 따른 clear zone 형성이 확인되 었다 Serratia sp. JM은 형태적, 생리.생화학적 특 성과 유기물 동화는 SUCCIniC, urea 및 pyruvic 산 을 제외하고는 공시 균주인 Serratia marcescens ATCC 27117과 유사하였으며, tetracyclin에 대해 서는 항생제에 대한 내성을 가지고 있었으나, kanamycin과 chloramphenicol에 대해서는 내성을 가지지 않았다. Serratia sp. JM의 chitinase 생성에 따른 최적온도와 pH는 $30^{\circ}C$ 와 7.5로 냐타났다. Serratia sp. JM은 120시간까지는 배양 시간이 증가 함에 따라 chiti-nase 생성과 pH는 점차 증가하였으 나, 배양 120시간 이후에는 chitin 분해에 따른 acetic acid의 축적에 따라 chitinase 생성과 pH는 감소 하였다.

• 한국미생물·생명공학회지
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• 제19권5호
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• pp.450-455
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• 1991

Serratia marcescens ATCC 21074로부터 돌연변이 유도에 의해 protease 생산능이 높은 ampicillin 耐性의 변이주 Serratia sp. SMNT-1을 분리하였다. 이 균주는 原 균주에 비하여 約 11배 정도 높은 protease 생산성을 나타내었다. 이 효소는 pH 9.0, $40^{\circ}C$에서 최대 활성을 나타내었으며, 저온하에서는 pH 6.0-10.0 범위에서 안정하였으나, $37^{\circ}C$, 알카리 조건하에서 불안정하였다. 이 효소는 $60^{\circ}C$에서 10분간 열처리시 실활되었다.

• Applied Biological Chemistry
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• 제42권3호
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• pp.181-187
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• 1999

경주지역의 토양으로부터 Fusarium 속 식물병원균에 길항력을 갖는 chitinase 생산성 길항미생물을 분리할 수 있었으며, 이를 분류학적으로 동정하여 본 결과 Serratia proteamaculans 3095로 동정할 수 있었다. 이 균주가 생성하는 chitinase의 생성조건을 조사한 결과 탄소원으로 colloidal chitin이 가장 좋았으며 그 최적 농도는 0.15%이었고, glucose에 의해 chitinase 생산 유도를 억제받는 효소임을 알 수 있었다. 질소원에 의한 영향은 $(NH_4)_2SO_4,\;(NH_4)Cl$, peptone 등에 의해 chitinase 생산성이 증가되었고, $(NH_4)_2SO_4$와 peptone을 각각 0.1%씩 첨가하였을 때 chitinase 생산이 가장 좋았다. 또한 시드름병균 Fusarium oxysporum을 대상으로 in vitro, in vivo pot 실험을 통해 Serratia sp. 3095의 강한 방제력을 검증할 수 있었다.

• 한국미생물·생명공학회지
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• 제27권2호
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• pp.96-101
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• 1999

Various side-chains are introduced to the 7-amino position of 7-aminocepha-losporanic acid (7-ACA) to make semi-synthetic cephalosporin antibiotics. In order to convert cephalosporin C (CPC) to 7-ACA, two enzymatic reactions are generally imployed. Glutary1-7-aminocephalosporanic acid (Gl-7-ACA) acylase is involved in the second step where the reaction intermediate, Gl-7-ACa is converted into 7-ACA. It was recently reported that CPC amidase can convert CPC directly into 7-ACA in a single enzymatic reaction. A study was undertaken to screen microorganisms conferring enzyme activity to convert Gl-7-ACA or CPC into 7-ACA by one or two enzymatic reactions. In order to screen the microorganisms rapidly, a non-$\beta$-lactam model compund, glutaryl-$\rho$-nitroanilide, was utilized in an early stage, thereafter the selected microorganisms were examined with real substrates. One microorganism exhibiting both Gl-7-ACA acylase and CPC amidase activities was obtained by the colorimetry method and HPLC assay, and was identified as a strain of Serratia species, designated as Serratia sp. N14.4. The optimal fermentation conditions for Serratia sp. N14.4 was pH9.0 and 3$0^{\circ}C$.

• Journal of Microbiology and Biotechnology
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• 제19권11호
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• pp.1431-1438
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• 2009

The role of plant growth-promoting rhizobacteria (PGPR) in the phytoremediation of heavy-metal-contaminated soils is important in overcoming its limitations for field application. A plant growth-promoting rhizobacterium, Serratia sp. SY5, was isolated from the rhizoplane of barnyard grass (Echinochloa crus-galli) grown in petroleum and heavy-metal-contaminated soil. This isolate has shown capacities for indole acetic acid production and siderophores synthesis. Compared with a non-inoculated control, the radicular root growth of Zea mays seedlings inoculated with SY5 can be increased by 27- or 15.4-fold in the presence of 15 mg-Cd/l or 15 mg-Cu/l, respectively. The results from hydroponic cultures showed that inoculation of Serratia sp. SY5 had a favorable influence on the initial shoot growth and biomass of Zea mays under noncontaminated conditions. However, under Cd-contaminated conditions, the inoculation of SY5 significantly increased the root biomass of Zea mays. These results indicate that Serratia sp. SY5 can serve as a promising microbial inoculant for increased plant growth in heavy-metal-contaminated soils to improve the phytoremediation efficiency.

• 식물병연구
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• 제21권3호
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• pp.222-226
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• 2015

The chitinase-producing bacterial strain C-1 is one of the key chitinase-producing biocontrol agents used for effective bioformulations for biological control. These bioformulations are mixed cultures of various chitinolytic bacteria. However, the precise identification, biocontrol activity, and the underlying mechanisms of the strain C-1 have not been investigated so far. Therefore, we evaluated in planta biocontrol efficacies of C-1 and determined the draft genome sequence of the strain in this study. The bacterial C-1 strain was identified as a novel Serratia sp. by a phylogenic analysis of its 16S rRNA sequence. The Serratia sp. C-1 bacterial cultures showed strong in planta biocontrol efficacies against some major phytopathogenic fungal diseases. The draft genome sequence of Serratia sp. C-1 indicated that the C-1 strain is a novel strain harboring a subset of genes that may be involved in its biocontrol activities.

• KSBB Journal
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• 제13권4호
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• pp.431-437
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• 1998

Optimal media and cultural conditions for the production of prodigiosin-like pigment were established using Serratia sp. KH-95. Glucose and phosphate(K2PO4) stimulated the cell growth, but inhibited the production of pigment at concentration levels of above 10 g/L and 2.0 g/L, respectively. Addition of soy been oil or rice oil to the production medium accelerated cell growth up to more than 2-3 times, but the production of prodigiosin increased about 15-20% in spite of the good cell growth. The effect of pH on the production of pigment was investigated in a 5 liter-bioreactor. When the pH of culture broth was maintained below 8.0, most of pigment was attached to the surface of cells. When the pH of culture broth was above 8.5, however, about 70% of total pigment was suspended in the supernatant of the broth. The cell growth and production of pigment were inhibited at dissolved oxygen concentration of below 10% of air-saturation.

• 한국미생물·생명공학회지
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• 제26권4호
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• pp.283-289
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• 1998

토양으로부터 다량의 적색 색소를 생산하는 KH-95 균주를 분리하였다. 형태학적 특성 및 생리학적 특성을 조사한 결과 KH-95는 Serratia sp.로 동정되었다. 본 균주가 생산하는 적색색소를 용매 추출 및 실리카겔 칼럼 크로마토그라피를 이용하여 분리, 정제 후 기기분석을 수행한 결과 prodigiosin의 일종으로 밝혀졌다. Serratia sp. KH-95 균주가 prodigiosin계 적색색소를 생산하기 위한 최적 조건은 배양온도 28$^{\circ}C$, 초기 pH는 7.0였으며 배지 조성을 검토 결과 카제인이 색소의 생산에 가장 적합하였다. 대두유, 미강유와 같은 oil을 제외한 대부분의 탄소원과 이용하기 쉬운 질소원인 효모즙, 육즙, 펩톤 등은 균체의 생장은 촉진 하였으나 색소 생산을 현저하게 저해하였다. 최적화된 배지 및 배양조건에서 대두유를 탄소원으로 첨가할 경우 3,380 mg/l의 많은 적색색소가 생산되었다.

• 한국미생물·생명공학회지
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• 제20권3호
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• pp.280-288
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• 1992

Serratia marcescens ATCC 21074 균주가 세포밖으로 분비하는 metalloprotease 유전자를 대장균으로 클로닝하고 그 발현을 살펴보았다 Serratia marcescens ATCC 21074 균주의 염색체 DNA를 제한효소 HindIII로 절단하고 아가로스 전기영동 후 32P로 표지된 합성 oligonucleotide를 사용하여 southern hybridization한 결과 4.0Kb의 DNA 절편에 metalloprotease가 존재함을 알 수 있었다. 4.0Kb 염색체 DNA 절ㅊ편을 분리하여 pUC19에 연결한 후 대장균으로 transformation하였다.

• 한국미생물·생명공학회지
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• 제47권3호
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• pp.459-464
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• 2019

This study aimed to identify and characterize the antimicrobial activity of prodigiosin produced by Serratia sp. $PDGS^{120915}$ isolated from stream water in Busan, Korea; the identification was performed using phonological, biochemical, and molecular techniques, including 16S rRNA sequence analysis. Prodigiosin from the bacterial culture was purified by high-performance liquid chromatography (HPLC), and its antimicrobial activity and minimum inhibitory concentrations (MICs) were evaluated against 10 intestinal pathogenic gram-positive and negative bacteria. The results revealed that the isolated prodigiosin exhibited high antimicrobial activity against Listeria monocytogenes, Bacillus cereus, Pseudomonas aeruginosa, Salmonella typhimurium, Staphylococcus aureus, and Vibrio parahaemolyticus; further, the isolated prodigiosin showed minimum inhibitory concentrations (MICs) between $3{\mu}g/ml$ and 30 mg/ml, but they were not active against Bacillus subtilis, Enterococcus faecalis, Klebsiella pneumonia, and Escherichia coli. In conclusion, prodigiosin isolated from Serratia sp. $PDGS^{120915}$ showed high antimicrobial activity against intestinal pathogenic bacteria and has potential applications in the development of new antimicrobial agents.