• Applied Microscopy
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• 제24권1호
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• pp.41-58
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• 1994

The present studies were designed to determine the feasibility of using the rat tracheal epithelium as models for induction of aging. The ultrastructural and cytochemical changes of tracheal epithelium were investigated in rats at ages of five, twelve and twenty four months. Some major changes in the tracheal epithelium with advancing age were observed by electron microscopy. The results were summarized as fellow: 1. With the advance of age, lysosome, vacuole and multivesicular bodies were increased in number and numerous myelinoid bodies were observed in cytoplasm of ciliated cells. 2. In goblet cell, serous cell and brush cell lysosome and myelinoid bodies were increased in number with the advance of age, and an myelinoid bodies was often found within the secretory granule. 3. Cytochemical studies showed that acid phosphatase activities was observed in multivesicular bodies and lysosome, strong activities with the advance of age. And alkaline phosphatase activity are observed in microvilli, granule and lateral membrane of secretory granule cells, and strong activities with age. Consequently suggest that with the advance of age, tracheal epithelium show ultrastructural and cytochemical alteration of some kind of cell organelles in all kind of cell.

• 한국수산과학회지
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• 제53권4호
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• pp.628-636
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• 2020

Streptococcosis in the olive flounder Paralichthys olivaceus can be caused by Streptococcus parauberis. We compared three routes of administration for experimental injections of the S. parauberis 19FBSPa0003 strain in the olive flounder. Pathological changes were observed during the experimental infection. Inflammation of the serous membrane in the liver, intestine, spleen and heart was the major pathological change found in the infected olive flounder. No mortality was observed in fish that received intraperitoneal (IP) injection at less than 1×10⁴ colony-forming unit (CFU)/fish. The lethal dose 50 for olive flounder, given an intravenous (IV) injection, was 7.94×10⁴ CFU/fish. Fish with a higher concentration of IV injected S. parauberis (1×10⁸ CFU/fish) died within a maximum of two days. However, serious necrosis and bacterial proliferation in ellipsoidal cells of the spleen and heart tissues were found in moribund or dead fish, 1-2 days after IV injection. Similar histopathological signs were observed in olive flounder inoculated by subcutaneous (SC) infected and naturally infected. In addition, SC was also strongly associated with bacteria concentration and cumulative mortality rate. Based on these results, SC is the recommended method for artificial infection by S. parauberis in the olive flounder.

• 한국수정란이식학회지
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• 제22권4호
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• pp.229-234
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• 2007

This study was undertaken to determine the normal appearance of the postpartum uterine involution. Postpartum changes in uterine shape, architecture, echogenicity and diameter were monitored with ultrasonography in 8 Miniature Schnauzer dogs. Vaginal discharge was observed visually during the 3 weeks of postpartum. A large amount of viscous dark green discharge changed progressively to a small amount of transparent discharge. In the uterine shape, the transverse images were crescent or polygonal at the beginning, but became circular after 16 days. At postpartum day 24, the longitudinal images of placental and interplacental sites were similar to each other The echogenicity of uterine structure was clearly distinguishable among the hyperechoic serous membrane, hypochoic myometrium, hyperechoic endometrium, and anechoic uterine contents during the first week postpartum, but afterward the interluminal boundaries and echogenicity became obscure gradually. In addition, the anechoic substance of the endometrial cavity was not observed after postpartum day 28. In the uterine diameter, the diameter of placental sites decreased markedly from 24 mm at the first day postpartum to 13 mm at day 7, and the diameter of interplacental sites decreased from 15 mm at postpartum day 1 to 10 mm at day 7. At postpartum day 65, the uterus (6.4 mm) changed little and had a uniform homogenous echo, and thus it was confirmed that uterine involution was completed at that time in Miniature Schnauzer dogs.

• 한국어류학회지
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• 제28권1호
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• pp.19-27
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• 2016

은대구의 소화관 상대길이 비는 1.52 (n=12)이며, 소화관은 위의 후방부에 5~6개의 유문수를 가진다. 점막주름의 형태는 식도와 위에서는 미분지형이지만 장에서는 분지형이다. 횡단면에서 소화관은 조직학적으로 점막층, 점막하층, 근육층 및 장막으로 구분할 수 있다. 식도의 점막상피층은 단층이며, 원주섬모상피세포들과 점액세포들로 구성된다. 위 점막층의 위선은 관상선으로 주세포, 벽세포 및 뮤신분비세포들로 구성된다. 뮤신분비세포는 원주형으로 AB-PAS (pH 2.5) 반응에서 분홍색과 푸른색을 나타내는 분비과립을 가진다. 장의 점막상피층은 단층이며, 원주섬모상피세포들과 배상세포들로 구성된다. 점막하층은 소성결합조직층으로 주로 교원섬유들로 구성되며, 식도에서 잘 발달되어 있다. 소화관의 근육층은 종주근층과 환상근층으로 구분되며, 위에서 잘 발달되어 있다. 간은 다수의 소엽구조와 담관들로 이루어져 있으며, H-E 염색에서 간세포의 세포질은 호산성이며, 핵과 인은 호염기성을 보였다. 췌장조직은 소화관 주변의 지방조직에 산재하며, 다수의 외분비세포들로 구성된 포상선이었다. H-E 염색에서 외분비선세포의 세포질은 호염기성을 나타내며, 다수의 호산성 전효소 과립들을 함유한다.

• 한국수산과학회지
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• 제32권5호
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• pp.642-648
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• 1999

감성돔, Acanthopagrus schlegeli의 소화관은 식도, 위, 장, 항문 그리고 위의 유문부 말단에서 유래한 4$\~$5개의 맹낭 형태인 유문수로 이루어져 있었다 어체 체장에 대한 식도에서 항문까지의 소화관 길이의 비 (RLG: relative length of gut)는 1.04(n=10)였다. 소화관의 조직층은 장막, 근육층, 점막하층, 점막층으로 구성되는데, 점막하층의 발달은 전체적으로 매우 미약했다. 식도 점막주름의 형태는 규칙적인 분지형이었으며, 점막근층은 아주 잘 발달되어 있었다. 점막상피층은 입방형 또는 원주형 상피세포와 점액분비세포로 구성되며, 상피세포의 자유면에서 미세융모의 관찰은 어렵다. 위점막주름의 형태는 규칙적인 미분지형이며, 위의 근육충과 점막근층은 잘 발달되어 있었다. 점막충의 표면상피에서는 미세융모가 관찰되며, 위선과 분비과립은 체부에서 가장 잘 발달되어 있었다 유문수와 장의 점막주름은 불규칙한 분지형이다 장은 점막주름의 길이와 조직학적인 특징에 의해 전장부, 중장부, 후장부의 구분이 가능하였다. 유문수와 장의 선조연과 배상세포는 후장부에서, 영양분흡수세포는 중장부에서 가장 발달된 양상을 보였다.

• 대한핵의학회지
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• 제1권1호
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• pp.37-54
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• 1967

The changes in histopathology of various organs and growth inhibition of the chick embryos incubated with radioactive sulfur ($^{35}S$) were experimentally studied. The various doses of $^{35}S$ were injected into the yolk sac at different intervals and the weight changes of the embryos were evaluated to determine the growth inhibition rates. The embryos sacrified on various incubation days were used for the study of histopathological changes in organs such as the bone, liver, kidney, gonad, and eye. Following were the results: 1) The weight changes of the $^{35}S$ treated groups were as follows: i. Embryos treated on the 5 th incubation day: No weight changes were noted on the 8th incubation day, however, the growth inhibition rate of 32.1% was noted in the group treated with $50{\mu}C$ and of 38.2% in the group treated with $150{\mu}C$ on the 12th incubation day. The rates were 9.1 and 12.1% on the 15th incubation day, and 6.5 and 10.6% on the 18th incubation day respectively. ii. Embryos treated on the 8th incubation day: The growth inhibition rates on the 12th, 15th and 18th incubation days in the groups treated with $50{\mu}C$ were 20.9, 25.9 and 18.8% and in those treated with $150{\mu}C$ were 20.0, 14.9 and 16.9% respectively. iii. Embryos treated on the 12th incubation day: The growth inhibition rates on the 15th and 18th in the groups treated with $50{\mu}C$ were 13.6 and 21.1% and in those treated with $150{\mu}C$ were 26.7 and 6.5% and in those treated with $250{\mu}C$ were 10.6 and 12.6% respectively. iv. Embryos treated on the 15th incubation day: The growth inhibition rates on the 18th in the groups treated with $50{\mu}C$ were 6.5% and in those treated with $150{\mu}C$ were 10.1% and in those treated with $250{\mu}C$ were 8.5% respectively. In summary, the longer the incubation days, the less the growth inhibition rates. II) The histopathological changes in the various organs were as follows: i. Bone: Hyperplasia and edematous changes of the bone cavity, irregular distribution of immature granular cells and increased number of the myeloblast, megakaryocyte and reticuloendothelial cells were noted. ii. Liver: The embryos treated with $150{\mu}C\;of\;^{35}S$ on the 8th incubation day showed necrosis and nucleolysis of the liver cell and abnormal enlargement of sinusoid on the 12th incubation day. The longer the incubation days, the more severe the changes such as the pyknotic artophy of the liver cells and heterochromatism. The embryos treated on the 5th incubation day with 50 and $150{\mu}C\;of\;^{35}S$ showed little changes, but sight enlargement and accumulation of serous fluid in the sinusoid on the 8th incubation day. iii. Kidney: No particular changes except atrophic changes of epithelium were noted in early stage, however, the infiltration of the granular cell and monocyte into the cortex and pyknotic changes of vascular glomeruli were noted in later stage. These changes were not closely related to the doses of $^{35}S$ given. iv. Gonad: The degenerative changes such as destruction of the immature germ cells, hyperplasia and vacuolization of the stroma were noted in testis and ovary. v. Eye: A slight distortion of the cornea and sclera was noted. The hypertrophy of inner layer and blood cell infiltration into the vascular layer of the choroid membrane were noted in embryo groups on the 12, 15 and 18th incubation days.

• 한국수산과학회지
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• 제33권2호
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• pp.129-136
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• 2000

부화직후 자어는 전장 2.0 mm (n=10)이며, 약 $1,000{\times}590 {mu}m$ 크기의 난황낭과, 입방상피로 구성된 직선상의 소화관을 가진다. 전장 $3.5mm$ 자어의 소화관은 회전되어 있으며, 식도, 위, 장의 구분이 가능하며, 췌장에서는 외분비선이 관찰된다. 그리고 이 시기에 후장부에서는 점막주름과 호산성과립세포 및 선조연이 관찰된다. 전장 3.7mm시기에 난황은 완전 흡수되며, 전장부의 상피층 정단면에서 선조연이 구분된다. 전장 4.0mm 시기에 위에서 점막 주름의 형성과 췌장의 외분비선세포의 세포질에서 효소원과립이 확인되었다. 전장 $4.5{\~}5.0mm$ 시기에 간소엽의 구분이 가능하며, 장과 식도에서는 PAS에 양성을 보이는 점액분비세포들이 관찰된다. 전장 $6.3{\~}7.0mm$ 자어의 식도와 장에서는 조직충의 구분이 가능하다. 전장 9.0mm 시기에 위는 분문부, 체부, 유문부의 구분이 가능하며, 위체부에서 위선이 관찰되기 시작한다. 식도와 장의 조직학적 구조가 성체와 유사한 시기는 전장 $10.0{\~}11.0mm$의 치어기이며, 위는 전장 15.0mm에 성체와 유사한 조직학적 구조를 보였다. 본 연구 결과 감성돔의 기능적 소화관은 전장 15.0{\~}l7.0mm$의 치어기에 완성된다고 할 수 있다.