• 한국동물위생학회지
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• 제32권2호
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• pp.139-146
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• 2009

Bovine brucellosis is a zoonosis, long incubation period and chronic infectious disease, usually caused by Brucella abortus. This study was carried out to investigate the biotyping and biochemical characterization of B. abortus isolated from 208 farm 871 korean cattle and holstein diagnosed brucellosis by serological positive in Gyeongbuk province during the period from 2002 to 2006. B. abortus was isolated from 124 (14.2%) of 871 cattle, and isolated 110 (13.4%) of 820 Korean cattle and 14 (27.5%) of 51 holstein in breed. The uterus of korean cattle was isolated in 8 (17.8%) of 45 cattle and supramammary lymph none of holstein was isolated 11 (68.8%) of 16 cattle. 101 (12.5%) of 810 serological positive blood samples were isolated B. abortus. The isolation rate of B. abortus was correlated with antibody titers. The biochemical characterization of isolates was non-hemolytic, production of H$_2$S, oxidase-positive, catalase-positive, hydrolyzation of urea and growth of basic fuchsin dye medium. As a result, all of isolates was identified B. abortus bv 1. 124 isolates were susceptible to ampicillin, lincospectin, amikacin, gentamicin, kanamycin, neomycin, streptomycin, tetracycline, ciprofloxacin, norfloxacin and enrofloxacin.

• 한국동물위생학회지
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• 제37권3호
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• pp.185-190
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• 2014

We investigated the serological prevalence of avian metapneumovirus (AMPV), avian reovirus (ARV), Mycoplasma gallisepticum (MG) and Mycoplasma synoviae (MS) in 760 broiler breeder (38 flocks), in the Jeonbuk province in 2013. This study was conducted to evaluate the immune and infection status of the broiler breeder flocks against AMPV, ARV, MG, MS by an enzyme-linked immunosorbent assay (ELISA). Serological test for AMPV were positive 37 (97.3%) flocks and 712 (93.6%) broiler breeder and geometric mean antibody titers were $16,350{\pm}10,195$, ARV were high positive rate 100% (38/38) flocks and 97.8% (743/760). The seropositive flocks against MG were 71.1% (27/38) and the geometric mean antibody titers were $2,474{\pm}2,045$, whereas the rates of positive flocks against MS were 50.0% (19/38) and the geometric mean antibody titers were $1,469{\pm}1,230$.

• 대한수의학회지
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• 제27권1호
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• pp.47-52
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• 1987

The serological typing of staphylococcus hyicus subsp. hyicus isolated from pigs with exudative epidermitis and healthy pigs was attempted by slide agglutination technique with absorbed sera prepared from antisera against swine strains of the organism. Of 204 Staph. hyicus subsp. hyicus isolated, 202 (99.0%) were classified into 6 serotypes tentatively designated as A, B, C, D, E and F. The organisms were found to possess common antigens and a strain specific antigen. In 26 epizootics of exudative epidermitis, type A was found in 4(15.4%), type B in 12(46.2%), type C in 9(34.6%) and type E in 1(3.8%). All of the piglets of each affected litter were infected by serologically identical strain. In 178 strains isolated from the skin of healthy pigs, type A was found in 14.1%, type B in 23.0%, type C in 30.3%, type D in 1.7%, type E in 7.9% and type F in 21.9%, and 2 strains were untypable. There was a difference in the occurrence of serotypes of Staph. hyicus subsp. hyicus among the farms examined.

• 미생물학회지
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• 제12권4호
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• pp.180-187
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• 1974

Of the Asp. spp. isolated by the Institute of Applied Microbiology, Kon-Kuk University, 7 strains were selected for the study of the immunological differencences among them using gel precipitation test. The strains were the following types : 1 type of flavus and 2 types of oryzae were isolated from Meju ; 1 type of flavus from Nuruk ; and each one type of flavus, parasiticus and oryzae from Kokja.Asp.flavus from ATCC, Asp. parasiticus nad Asp. niger NRRL strains were also used in the study as a standard. From this study, several points can be raised ; 1) There was no common antigenic property between Asp. niger and Asp. flavus, because of no formation of reaction line. Therefore, all strains could be easily distinguished. 2) There was common antigenic property, that is, the formation of reaction line between Asp. flavus and Asp. parasticus. Accordingly two strains could not be easily distinguished by the gel precipitation test. 3) Each type of oryzae, parasiticus and flavus of Asp. flavus group had common antigen one another as well as specific antigens only in the difference of the reaction lines, so they could be easily identified in the gel precipitation test. 4) Each isolated strain from Meju and Nuruk appeared to be identical. 5) It was shown that the gel precipitation test of serological methods was very useful for the classification of Asp. spp.

• 대한수의학회지
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• 제39권6호
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• pp.1106-1111
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• 1999

In a kenel of 62 dogs, 33 were diagnosed as infected dogs Brucella canis by serological test and blood cultures in a large kennel in Chonnam area. Twenty eight of 33 dogs were treated with combined antibiotics therapy consisting of tetracycline and dihydrostregtomycin. After the first treatment, all dogs became abacteremic and serologic titers declined. Abortion due to B canis infection could be prevented by antibiotic therapy during pregnancy. However, sequential antibiotics therapy for 4 weeks did not eradicate B canis from affected bitch. According to results of serological and blood culture tests, effect of antibiotics treatment respectively revealed that 17 of the originally infected dogs were cure with second therapy schedule at 2 months and 27 of that with 6 months after second therapy.

• 대한수의학회지
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• 제32권1호
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• pp.57-61
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• 1992

Borna disease(BD) virus 특이항체검출에 대한 세 가지 혈청진단법(간접형광항체법, 세포효소면역반응법, 혈청중화시험)의 정확도를 비교하기 위해 BD virus를 실험적으로 감염시킨 273수의 토끼의 혈청으로 시험하였다. 혈청중 간접형광항체법에 의하여 판정된 123혈청들은 모두 세포효소면역반응법에 의해서 양성으로 판정되었으나 혈청중화시험법에 의해서는 단지 27혈청에서만 양성으로 판정되었다. 혈청중화시험법은 간접형광항체법과 세포효소면역반응법에 비하여 민감도가 훨씬 낮게 나타나 BD virus의 혈청학적 연구에는 간접형광항체법 및 세포효소면역반응법의 활용도가 높을 것으로 생각된다.

• 한국동물위생학회지
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• 제23권3호
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• pp.271-279
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• 2000

This study was carried out to provide the fundamental information for development of proper vaccination program against infectious bursal disease(IBD) to the local chicken farms. The antigen detection was peformed from 8 samples of bursa of Fabricius with agar gel precipitation(AGP) and indirect immunofluorescent assay(IFA), And also, the antibodies in serum samples were detected by the various serological methods such as commercial ELISA assay, AGP and virus neutralization(VN) test. 1. The antigen detection rates were 25% for AGP which is 2 out of 8 farms and 10 out of 40 bursas, and 25% which Is 2 out of 8 farms and 20% 8 out of 40 bursas for IFA, respectively. 2. The mean titer of maternal antibody (>3,000) existed until 10 days of the age with ELISA-GMT. 3. The antibody positive rates which are over 80% showed until 5 days of the age with ELISA and at 10 days of the age with AGP except one, but none of them showed from 1 day of the age. This report came to conclusions that both the protective maternal antibody titers and the antigen positive rates were significant until at the 10 days of the age.

• Journal of Plant Biology
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• 제28권2호
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• pp.95-104
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• 1985

층층나무속(Cornus) 식물 수종과 그 근연군의 계통학적 유연관계를 밝히기 위하여 종자단백질을 이중면역확산법, 사전포화처리법, 방사상면역확산법, 면역학적 전기영동법 등으로 분석함으로 혈청학적추가자료를 얻었다. Cornus florida 면역혈청을 사용한 침전반응에서 C. drummondi와 C. stolonifera는 혈청학적 유사도가 매우 높았고, C. amomum과 C. recemosa는 다음으로 높았다. Cornus와의 근연군에 해당하는 분류군 중에서 Nyssa(Nyssaceae)가 가장 가까운 근연성을 나타냈고, Corokia (Cornaceae 또는 Saxifragaceae)와 Griselinia는 매우 낮은 근연성을 보여서 Cornaceae에의 소속을 지지하고 있지 않다. 그러나 그들의 독립된 과로서 구분하기 위해서는 많은 다른 분류학적 증거가 필요할 것으로 본다. 면역학적 전기영동법과 사전포화처리법의 조합된 기술은 비교적 정교한 종자단백질 분석이 가능했다.

• The Plant Pathology Journal
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• 제36권6호
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• pp.651-657
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• 2020

Lotus is one of the most important aquatic vegetables in China. Previously, we detected sweet potato latent virus from lotus (SPLV-lotus) and found that it has highly significant sequence diversity with SPLV-sweet potato isolates (SPLV-sp). Here, we developed serological methods for the detection of SPLV-lotus in Chinese lotus cultivation areas. Based on the high sensitivity of SPLV-lotus coat protein antiserum, rapid, sensitive and large-scale diagnosis methods of enzyme-linked immunosorbent assay (ELISA) and dot blot in lotus planting area were developed. The established ELISA and dot blot diagnostic methods can be used to detect SPLV-lotus from samples successfully. And our results also showed that the SPLV-lotus and sweet potato isolates appeared clearly distinction in serology. Our study provides a high-throughput, sensitive, and rapid diagnostic method based on serology that can detect SPLV on lotus, which is suggested to be included in viral disease management approach due to its good detection level.

• 대한수의학회지
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• 제42권1호
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• pp.73-80
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• 2002

An enzyme-linked immunosorbent assay (ELISA) using purified hemagglutinin of swine influenza virus (H1N1) as antigen was developed for detection of antibody to avian influenza virus (AIV). The sensitivity and specificity of a developed and commercial available ELISA kits were compared with those of agar gel precipitation (AGP) test and hemagglutination inhibition (HI) test using sera collected from chickens under condition of field exposure. The concentration of antigen, serum dilution and concentration of enzyme-conjugated secondary antibody in developed ELISA (S-ELISA) were 0.5ug/100ul, 1:200 and 0.03ug/100ul, respectively. The correlation coefficients between S-ELISA and commercial ELISA and HI titers were 0.419 and 0.533, respectively. A significant correlation (p < 0.01) was not found between HI and ELISA titers. The S-ELISA was found to be as more sensitive and specific than the AGP test, showing 86.8% sensitivity and 85.3% specificity. It is suggested that the ELISA using the SIV as antigen may be useful method as an investigating tool for AIV serological surveillance.