• Journal of the Korean Society of Food Science and Nutrition
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• v.9 no.1
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• pp.59-73
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• 1980

A detailed procedure was described for the isolation of cratine kinase (ATP-Creatine phosphotransferase, E. C. 2. 7. 3. 2.) from the muscle of the snake Bungarus fasciatus. The original isolation procedure of Kuby et al. for the rabbit muscle enzyme has been modified and extended to include a chromatographic step. The properties of the enzyme have been investigated and kinetic constants for the reverse reactions determined as the followings: 1) A molecular weight of the enzyme was determined by gel filteration on Sephadex G-100 and by electrophoresis on SDS-polyacrylamide was 86,000. 2) Two reactive sulphydryl groups were detected with dithiobis nitrobenzoic acid (DTNB). 3) The nucleotide substrate specificity in the reverse reaction was determined as ADP*2'-dADP＞GDP＞XDP＞UDP with magnesium as the activating metal ion. 4) The order of the metal specificity in the reverse reaction Mg>Mn>$Ca{\sim}Co$ was determined with ADP as substrate. 5) A detailed kinetic analysis was carried out in the reverse direction with $MaADP^-$ as the nucleotide substrate. Initial velocity and product inhibition studies($MaADP^{2-}$ competitive with respect to MgADP- and noncompetitive with respect to $N-phosphorycreatine^{2-}$ ; Creatine competitive with respect to $N-phosphorycreatine^{2-}$ and noncompetitive with respect to Ma $ADP^-)$ indicated that the reaction obeyed a sequential mechanism of the rapid equilibrium random type.

• Geotechnical Engineering
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• v.12 no.3
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• pp.149-164
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• 1996

The sequential behavior of earth retaining structure is investigated by using soil springs in elasto -plastic soil. Mathematical model that can be used to construct the p-y curves for subgrade modulus is proposed by using piecewise linear function. The excavation sequence of retaining wall is analyzed by the beam -column method. Reliability on the developed computer program is verfied through the comparison between the prediction and the in -situ measuidments. It is concluded that the proposed method simulates well the construction sequence and thus represents a significant improvement in the prediction of deflections of anchored wall excavation. Based on the results the proposed method can be effectively used for the evaluation of the relative importance of the parameters employed in a sensitivity analysis.

• The Journal of Korean Physical Therapy
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• v.22 no.5
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• pp.109-115
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• 2010

Purpose: This study was to delineate the structural change of neural pathway after sequential motor learning using diffusion tensor imaging (DTI). Methods: The participants were 16 healthy subjects, which were divided by training (n=8) and control (n=8) group. The task for the training was the Serial Reaction Time Task (SRTT) which was designed by Superlab program. When the 'asterisk' shows up in the 4 partition spaces on the monitor, the subject presses the correct response button as soon as possible. The training group participated in the training program of motor learning with SRTT composed of 24 digits pattern in one hour per daily through 10 days during 2 weeks. Results: In the behavioral results the training group showed significant changes in the increase of response number and the reduction of response time than those of the control group. There was significant difference in the left inferior frontal area in the fractional anisotropy (FA) map of the training group in DTI analysis. Conclusion: Motor sequential learning as like SRTT may be needed to the learning of language and visuospatial processing and may be induced for the experience-dependent structural plasticity during short period.

• Bulletin of the Korean Chemical Society
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• v.31 no.4
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• pp.989-998
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• 2010

The titled dye of DBPI gives amplified spontaneous emission (ASE) with maximum at 580 nm upon pumping by nitrogen laser (${\lambda}_{ex}\;=\;337.1\;nm$). The ground state absorption cross section (${\sigma}_A$) and emission cross section (${\sigma}_E$) as well as effective emission cross section(${\sigma}^*_E$) have been determined. The electronic absorption spectra of DBPI were measured in ethanol and tetrahydrofuran at room and low temperature. DBPI displays molecular aggregation in water. The photochemical reactivity of DBPI was also studied in carbon tetrachloride upon irradiation with 525 nm light. The electrochemical investigation of DBPI dye has been carried out using cyclic voltammetry and convolution deconvolution voltammetry combined with digital simulation technique at a platinum electrode in 0.1 mol/L tetrabutyl ammonium perchlorate (TBAP) in two different solvents acetonitrile ($CH_3CN$) and dimethylformamide (DMF). The species were reduced via consumption of two sequential electrons to form radical anion and dianion (EE mechanism). In switching the potential to positive direction, the compound was oxidized by loss of two sequential electrons, which were followed by a fast dimerization and/or aggregation process i.e $EC_{dim1}EC_{dim2}$ mechanism. The electrode reaction pathway and the chemical and electrochemical parameters of the investigated compound were determined using cyclic and convolutive voltammetry. The extracted electrochemical parameters were verified and confirmed via digital simulation method.

• Journal of the Korean Chemical Society
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• v.68 no.3
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• pp.139-145
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• 2024

This study aims to elucidate the mechanism involved in the hydrolysis of the hexacyanoferrate(III) complex ion (Fe(CN)₆^3-) and the mechanism leading to the formation of Prussian blue (Fe^III₄[Fe_II(CN)₆]₃·xH₂O, PB) in acidic aqueous solutions at moderately elevated temperatures. Hydrolysis constitutes a crucial step in generating PB through the widely used single-source or precursor method. Recent PB syntheses predominantly rely on the single-source method, where hexacyanoferrate(II/III) is the exclusive reactant, as opposed to the co-precipitation method employing bare metal ions and hexacyanometalate ions. Despite the widespread adoption of the single-source method, mechanistic exploration remains largely unexplored and speculative. Utilizing UV-vis spectrophotometry, negative-ion mode liquid chromatography-electrospray ionization-mass spectrometry (LC-ESI-MS), and a devised reaction, this study identifies crucial intermediates, including aqueous Fe^2+/3+ ions and hydrocyanic acid (HCN) in the solution. These two intermediates eventually combine to form thermodynamically stable PB. The findings presented in this research significantly contribute to understanding the fundamental mechanism underlying the acid-catalyzed hydrolysis of the hexacyanoferrate(III) complex ion and the subsequent formation of PB, as proposed in the sequential mechanism introduced herein. This finding might contribute to the cost-effective synthesis of PB by incorporating diverse metal ions and potassium cyanide.

• Journal of Microbiology and Biotechnology
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• v.17 no.6
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• pp.960-967
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• 2007

The co-immobilization of Aspergillus niger glucose oxidase (GOD) with bovine liver catalase (CAT) onto florisil (magnesium silicate-based porous carrier) was investigated to improve the catalytic efficiency of GOD against $H_2O2$ inactivation. The effect of the amount of bound CAT on the GOD activity was also studied for 12 different initial combinations of GOD and CAT, using simultaneous and sequential coupling. The sequentially co-immobilized GOD-CAT showed a higher efficiency than the simultaneously co-immobilized GOD-CAT in terms of the GOD activity and economic costs. The highest activity was shown by the sequentially co-immobilized GOD-CAT when the initial amounts of GOD and CAT were 10 mg and 5 mg per gram of carrier. The optimum pH, buffer concentration, and temperature for GOD activity for the same co-immobilized GOD-CAT sample were then determined as pH 6.5, 50 mM, and $30^{\circ}C$, respectively. When compared with the individually immobilized GOD, the catalytic activity of the co-immobilized GOD-CAT was 70% higher, plus the reusability was more than two-fold. The storage stability of the co-immobilized GOD-CAT was also found to be higher than that of the free form at both $5^{\circ}C\;and\;25^{\circ}C$. The increased GOD activity and reusability resulting from the co-immobilization process may have been due to CAT protecting GOD from inactivation by $H_2O2$ and supplying additional $O_2$ to the reaction system.

• The Journal of Korean Physical Therapy
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• v.26 no.4
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• pp.234-239
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• 2014

Purpose: The purpose of this study is to use P300 latency to determine whether methods of motor learning in terms of massed and distributed practice can affect motor sequential learning in healthy adults. Methods: Twenty-four healthy subjects participated in this study. They were randomly allocated into three groups: a 10 minute, a 12 hour, and a 24 hour group. In the SRT task, eight numbers were adopted as auditory stimuli. During an experiment, participants were instructed to press the matching key as quickly and accurately as possible when one of the eight numbers was presented randomly. The subjects practiced for three sessions, each of which comprised five blocks of 40 serial reaction time tasks. While they practiced during these three sessions, P300 latency was measured. The data were analyzed using ANCOVA. Results: The P300 latency of Fz, Cz, and Pz decreased in all groups except for the Fz area of the 10 min group. Overall, the P300 latency of the 10 min group showed a smaller decrease compared with the 12 hr and 24 hr groups. Statistically, no significant differences in the Fz and Cz areas were observed among the three groups. The P300 latency in the Pz area of the 10 min group showed a significantly smaller decrease compared with the other groups. Conclusion: These findings suggest that short-term sequential motor training can alter brain functions such as the P300 latency. We also found that better acquisition of a motor skill was obtained with distributed practice of a task than with massed practice.

• Journal of the Korean Applied Science and Technology
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• v.39 no.5
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• pp.701-709
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• 2022

The purpose of this study was to compare the differences according to the visual selection and rotation order during sequential rotational jump for female dancers of a Korean ballet company by classifying them into take-off and landing sections. 10 subjects (age: 26.0±2.9 yrs, height: 163.4±3.3 cm, weight: 46.8±3.6 kg, ballet career: 12.3±5.9 yrs) participated in the study. Using a 3D motion analyzer and a force platform, the height of the body center and the ground reaction force during take-off and landing were measured. According to the visual condition (using both eyes, using left eye, using right eye) and rotation order (first rotation, second rotation), it was analyzed through repeated measurement two-way analysis. Height of the CM was higher in the first jump. In take-off, Fx was lateral force of left foot and medial force of right foot were strong in second rotation, and Fy was forward force was strong in first rotation of right foot. Fz was no significant. In landing, Fy showed backward force was strong when landing the second time from the left foot, and the backward force was strong when using the left sight from the right foot. Fz was strong on the second landing on the left foot and the first landing on the right foot.

• Macromolecular Research
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• v.14 no.3
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• pp.272-286
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• 2006

Dendritic hyperbranched poly(methyl methacrylate)s (PMMA)s, whose branched architectures resemble the 'dendron' part(s) of dendrimer, were synthesized by an iterative methodology consisting of two reactions in each iteration process: (a) a coupling reaction of u-functionalized, living, anionic PMMA having two tert-butyldimethylsilyloxymethylphenyl(SMP) groups with benzyl bromide(BnBr)-chain-end-functionalized PMMA, and (b) a transformation reaction of the introduced SMP groups into BnBr functionalities. These two reactions, (a) and (b), were repeated three times to afford a series of dendron-like, hyperbranched (PMMA)s up to third generation. Three dendron-like, hyperbranched (PMMA)s different in branched architecture were also synthesized by the same iterative methodology using a low molecular weight, functionalized 1,1-diphenylalkyl anion prepared from sec-BuLi and 1,1-bis(3-tert-butyldime-thylsilyloxymethylphenyl)ethylene in the reaction step (b) in each iterative process. Furthermore, structurally similar, dendron-like, hyperbranched block copolymers could be successfully synthesized by the iterative methodology using $\alpha$-functionalized, living, anionic poly(2-(perfluorobutyl) ethyl methacrylate) (PRfMA) in addition to $\alpha$-functionalized, living PMMA. Accordingly, the resulting block copolymers were comprised of both PMMA and PRfMA segments with different sequential orders. After the block copolymers were cast into films and annealed, their surface structures were characterized by angle-dependent XPS and contact angle measurements. All three samples showed significant segregation and enrichment of PRfMA segments at the surfaces.

• Computers and Concrete
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• v.31 no.5
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• pp.405-417
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• 2023

The submerged floating tunnel (SFT) is tethered by mooring lines anchored to the seabed, therefore, the structural integrity of the anchor should be sensitively managed. Despite their importance, reaction forces cannot be simply measured by attaching sensors or load cells because of the structural and environmental characteristics of the submerged structure. Therefore, we propose an effective method for estimating the reaction forces at the seabed anchor of a submerged floating tunnel using a structural pattern model. First, a structural pattern model is established to use the correlation between tunnel motion and anchor reactions via a deep learning algorithm. Once the pattern model is established, it is directly used to estimate the reaction forces by inputting the tunnel motion data, which can be directly measured inside the tunnel. Because the sequential characteristics of responses in the time domain should be considered, the long short-term memory (LSTM) algorithm is mainly used to recognize structural behavioral patterns. Using hydrodynamics-based simulations, big data on the structural behavior of the SFT under various waves were generated, and the prepared datasets were used to validate the proposed method. The simulation-based validation results clearly show that the proposed method can precisely estimate time-series reactions using only acceleration data. In addition to real-time structural health monitoring, the proposed method can be useful for forensics when an unexpected accident or failure is related to the seabed anchors of the SFT.