Journal of Microbiology and Biotechnology

The Korean Society for Microbiology and Biotechnology (한국미생물·생명공학회)
권호 표지

Simultaneous and Sequential Co-Immobilization of Glucose Oxidase and Catalase onto Florisil

  • Gul, Ozyilmaz (University of Mustafa Kemal, Faculty of Arts & Sciences, Department of Chemistry) ;
  • Tukel, S. Seyhan (University of Cukurova, Faculty of Arts & Sciences, Department of Chemistry)
  • Published : 2007.06.30
Download PDF
⟨ Previous Next ⟩

Abstract

The co-immobilization of Aspergillus niger glucose oxidase (GOD) with bovine liver catalase (CAT) onto florisil (magnesium silicate-based porous carrier) was investigated to improve the catalytic efficiency of GOD against $H_2O2$ inactivation. The effect of the amount of bound CAT on the GOD activity was also studied for 12 different initial combinations of GOD and CAT, using simultaneous and sequential coupling. The sequentially co-immobilized GOD-CAT showed a higher efficiency than the simultaneously co-immobilized GOD-CAT in terms of the GOD activity and economic costs. The highest activity was shown by the sequentially co-immobilized GOD-CAT when the initial amounts of GOD and CAT were 10 mg and 5 mg per gram of carrier. The optimum pH, buffer concentration, and temperature for GOD activity for the same co-immobilized GOD-CAT sample were then determined as pH 6.5, 50 mM, and $30^{\circ}C$, respectively. When compared with the individually immobilized GOD, the catalytic activity of the co-immobilized GOD-CAT was 70% higher, plus the reusability was more than two-fold. The storage stability of the co-immobilized GOD-CAT was also found to be higher than that of the free form at both $5^{\circ}C\;and\;25^{\circ}C$. The increased GOD activity and reusability resulting from the co-immobilization process may have been due to CAT protecting GOD from inactivation by $H_2O2$ and supplying additional $O_2$ to the reaction system.

Keywords

References

  1. Banu, S., G. M. Greenway, and R. A. Wheatley. 2005. Luminol chemiluminescence induced by immobilised xanthine oxidase. Anal. Chim. Acta 541: 91-97
  2. Bao, J., K. Furumoto, K. Fukunaga, and K. Nakao. 2001. A kinetic study on air oxidation of glucose catalyzed by immobilized glucose oxidase for production of calcium gluconate. Biochem. Eng. J. 8: 91-102 https://doi.org/10.1016/S1369-703X(00)00140-6
  3. Blaauwen, T., A. Lindqvist, J. Lowe, and N. Nanninga. 2001. Distribution of the Escherichia coli structural maintenance of chromosomes (SMC)-like protein MukB in the cell. Mol. Biol. 42: 1179-1188
  4. Blandino, A., M. Macias, and D. Cantero. 2001. Immobilization of glucose oxidase within calcium alginate gel capsules. Process Biochem. 36: 601-606 https://doi.org/10.1016/S0032-9592(00)00240-5
  5. Blandino, A., M. Macias, and D. Cantero. 2002. Modelling and simulation of a bienzymatic reaction system co-immobilised within hydrogel-membrane liquid-core capsules. Enzym. Microb. Technol. 31: 556-565 https://doi.org/10.1016/S0141-0229(02)00154-0
  6. Godjevargova, T., R. Dayal, and I. Marinov. 2004. Simultaneous covalent immobilization of glucose oxidase and catalase onto chemically modified acrylonitrile copolymer membranes. J. Appl. Polym. Sci. 91: 4057-4063 https://doi.org/10.1002/app.13617
  7. Greenfield, P. F., J. R. Kittrell, and R. L. Laurence. 1975. Inactivation of immobilized glucose oxidase by hydrogen peroxide. Anal. Biochem. 65: 109-124 https://doi.org/10.1016/0003-2697(75)90497-2
  8. Harborn, U., B. Xie, R. Venkatesh, and B. Danielsson. 1997. Evaluation of a miniaturized thermal biosensor for the determination of glucose in whole blood. Clin. Chim. Acta 267: 225-237 https://doi.org/10.1016/S0009-8981(97)00151-4
  9. Isaksen, A. and J. Adler-Nissen. 1997. Antioxidative effect of glucose oxidase and catalase in mayonnaises of different oxidative susceptibility. I. Product trials. Lebensm. Wiss. Technol. 30: 841-846 https://doi.org/10.1006/fstl.1997.0283
  10. Jung, S.-K., Y.-R. Chae, J.-M. Yoon, B.-W. Cho, and K.-G. Ryu. 2005. Immobilization of glucose oxidase on multi-wall carbon nanotubes for biofuel cell applications. J. Microbiol. Biotechnol. 15: 234-238
  11. Kleppe, K. 1966. The effect of hydrogen peroxide on glucose oxidase from Aspergillus niger. Biochemistry 5: 139-143 https://doi.org/10.1021/bi00865a018
  12. Kramer, L. and E. Stecknan. 1997. Coimmobilization of $L-{\alpha}-glycerophosphate$ oxidase with catalase and its application for the synthesis of dihydroxyacetone phosphate. Tetrahedron 53: 14645-14650 https://doi.org/10.1016/S0040-4020(97)01009-0
  13. Lowry, O. H., N. J. Rosebrough, A. L. Farr, and R. J. Rondall. 1951. Protein measurement with the folin phenol reagent. J. Biol. Chem. 193: 261-275
  14. Metodiewa, D. and H. B. Dunford. 1992. Spectral studies of intermediate species formed in one-electron reactions of bovine liver catalase at room and low temperatures. A comparison with peroxidase reactions. Int. J. Radiat. Biol. 62: 543-553 https://doi.org/10.1080/09553009214552461
  15. Miller, G. L. 1959. Use of dinitrosalicylic acid reagent for determination of reducing sugar. Anal. Chem. 31: 426-428 https://doi.org/10.1021/ac60147a030
  16. Nunez-Delicado, E., F. Garci-Carmona, and A. Sanchez- Ferrer. 2005. Evidence of p-quinoid enamine formation during the oxidative desamination of p-hydroxy-D-phenylglycine catalyzed by D-amino acid oxidase. J. Mol. Catal. B Enzym. 35: 7-13 https://doi.org/10.1016/j.molcatb.2005.04.014
  17. Ozyilmaz, G., S. S. Tukel, and O. Alptekin. 2007. Activity and storage stability of immobilized glucose oxidase onto magnesium silicate. Indian J. Biochem. Biophys. 44: 38-43
  18. Ozyilmaz, G., S. S. Tukel, and O. Alptekin. 2005. Kinetic properties and storage stabilities of CAT immobilized onto florisil. J. Mol. Catal. B Enzym. 35: 154-160 https://doi.org/10.1016/j.molcatb.2005.07.001
  19. Parpinello, G. P., F. Chinnici, A. Versari, and C. Ripont. 2002. Preliminary study on glucose oxidase-catalase enzyme system to control the browning of apple and pear purees. Lebensm. Wiss. Technol. 35: 239-243 https://doi.org/10.1006/fstl.2001.9851
  20. Pickering, G. J., D. A. Heatherbell, and M. F. Barnes. 1999. Optimising glucose conversion in the production of reduced alcohol wine using glucose oxidase. Food Res. Int. 31: 685- 692 https://doi.org/10.1016/S0963-9969(99)00046-0
  21. Podual, K., F. J. Doyle III, and N. A. Peppas. 2000. Glucosesensitivity of glucose oxidase-containing cationic copolymer hydrogels having poly(ethylene glycol) grafts. J. Contr. Release 67: 9-17 https://doi.org/10.1016/S0168-3659(00)00195-4
  22. Ramanathan, K., B. R. Jonsson, and B. Danielsson. 2001. Sol-gel based thermal biosensor, for glucose. Anal. Chim. Acta 427: 1-10 https://doi.org/10.1016/S0003-2670(00)01095-3
  23. Sisak, C., Z. Csanadi, E. Ronay, and B. Szajani. 2006. Elimination of glucose in egg white using immobilized glucose oxidase. Enzyme Microb. Technol. 39: 1002-1007 https://doi.org/10.1016/j.enzmictec.2006.02.010
  24. Tarhan, L. and A. Telefoncu. 1990. Characterization of immobilized glucose oxidase-catalase and their deactivation in a fluid-bed reactor. Appl. Biochem. Biotechnol. 26: 45- 47 https://doi.org/10.1007/BF02798392
  25. Tarhan, L. and A. Telefoncu. 1992. Effect of enzyme ratio on the properties of glucose oxidase and catalase immobilized on modified perlite. Process Biochem. 27: 11-15 https://doi.org/10.1016/0032-9592(92)80003-L
  26. Traitel, T., Y. Cohen, and J. Kost. 2000. Characterization of glucose-sensitive release systems in simulated in vivo conditions. Biomaterials 21: 1679-1687 https://doi.org/10.1016/S0142-9612(00)00050-8
  27. Trost, E. M. and L. Fischer. 2002. Minimization of byproduct formation during D-amino acid oxidase catalyzed racemate resolution of D/L-amino acids. J. Mol. Catal. B Enzym. 19-20: 189-195
  28. Tukel, S. S. and O. Alptekin. 2004. Immobilization and kinetics of catalase onto magnesium silicate. Process Biochem. 39: 2149-2155 https://doi.org/10.1016/j.procbio.2003.11.010
  29. Tzanov, T., S. A. Costa, G. M. Gubitz, and A. Cavaco-Paulo. 2002. Hydrogen peroxide generation with immobilized glucose oxidase for textile bleaching. J. Biotechnol. 93: 87- 94 https://doi.org/10.1016/S0168-1656(01)00386-8
  30. Vrbova, E. and M. Marek. 1990. Application of the Ugi reaction for the preparation of enzyme electrodes. Anal. Chim. Acta 239: 263-268 https://doi.org/10.1016/S0003-2670(00)83860-X
  31. Vrbova, E., J. Peckova, and M. Marek. 1993. Biosensor for determination of starch. Starch 45: 341-344 https://doi.org/10.1002/star.19930451003
  32. Williams, O. J. and K. D. Golden. 2002. Purification and characterization of ACC oxidase from Artocarpus altilis. Plant Physiol. Biochem. 40: 273-279 https://doi.org/10.1016/S0981-9428(02)01379-7