• Proceedings of the IEEK Conference
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• 2000.06b
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• pp.181-184
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• 2000

In this paper, we described a design of the input buffer system for efficiently dealing with MPEG audio bitstream to demux header and side information, audio data. In order to overcome the limitations of fixed-word manipulation in bitstream demuxing, we proposed a new variable length bit retrieval system with FSM sequencer supporting MPEG audio frame format, and serial buffer demuxing audio stream, FIFO circular buffer including header and side information.

• 제어로봇시스템학회:학술대회논문집
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• 1987.10b
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• pp.312-316
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• 1987

A simple hybrid circuit to control the two-phase AC motor of the nut-runners which employ the tightening torque control system is described in this paper. The circuit has emphasis on the low-cost implementation. The circuit constitutes of the V/F converter using a timer IC, the pulse width modulator using the fastening torque signal and the two-phase logic sequencer.

• Journal of Industrial Technology
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• v.11
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• pp.55-63
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• 1991

This paper presents a design of the accelerate circuit for the conversion of the vector font data into the bit-mapped image. Among the Bezier curve algorithm, the subdivision algorithm gives the good performance and easy hardware implementation. The sequencer is realized by the proprammable gate array and the processing unit is composed of EPLDs and TTL ICs.

• Proceedings of the Plant Resources Society of Korea Conference
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• 2018.10a
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• pp.25-25
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• 2018

The Fukushima Daiichi Nuclear Power Plant accident in March 2011 caused severe radioactive contamination in the surrounding environment. Since the accident, much attention has been paid to the biological and genetic consequences of organism inhabiting the contaminated area. The effect of radiation exposure on genetic mutation rates is little known, especially for low doses and in situ conditions. Evaluating DNA mutation by low levels of radiation dose is difficult due to the rare mutation event and lack of sequence information before the accident. In this study, correlations with air dose levels and somatic DNA mutation rates were evaluated using Next Generation Sequencer for the clonal plant, Phyllostachys edulis. This bamboo is known to spread an identical clone throughout Japan, and it has the advantage that we can compare genetic mutation rate among identical clone growing different air dose levels. We collected 94 samples of P. edulis from 14 sites with air dose rates from $0.04{\sim}7.80{\mu}Gy/h$. Their clonal identity was confirmed by analysis using 24 microsatellite markers, and then, sequences among samples were compared by MIG sequence. The sequence data were obtained from 2,718 loci. About ~200,000 bp sequence (80 bp X 2,718 loci) were obtained for each sample, and this corresponds to about 0.01% of the genome sequence of P. edulis. In these sequences, 442 loci showed polymorphism patterns including recent origin mutation, old mutation, and sequence errors. The number of mutations per sample ranged from 0 to 13, and did not correlate with air dose levels. This result indicated that DNA mutations have not accumulated in P. edulis living in the air doses levels less than $10{\mu}Gy/h$. Our study also suggests that mutation rates can be assessed by selecting an appropriate experimental approach and analyzing with next generation sequencer.

• Trans-
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• v.8
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• pp.55-78
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• 2020

Among various tools for pop music composition, what is most frequently used by K-Pop composers is the digital sequencer program, or DAW software (Digital Audio Workstation). When it comes to making commercial music such as K-Pop, the proportion of using these programs is extremely high. By using those software, composers come to undergo an entirely different art creation process. User interface (UI) of the DAW software has a profound effect on the process of making music, particularly vis-a-vis usability and visibility. This study is an attempt to analyze the Avid Pro Tool's UI with a focus on its inherent characteristics revealed in relation with the real effect throughout music composition. This study categorizes the UI elements in the Edit Window of the Pro Tools as Grid, Track, Slip, Clip, and Fade. Those categorized elements would be analyzed through Douglas Morgan's Creative-Process theory, thereby clarifying how visual interface of the DAW software functions in music composition practically.

• Journal of agriculture & life science
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• v.43 no.2
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• pp.31-38
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• 2009

The DNA sequencer is known to be more sensitive for the quality of template DNA, method of purification followed by sequencing reaction, and gel concentration. Therefore, we investigated optimal conditions for template preparation, purification, sequencing reaction, gel concentration, and injection medium. For plasmid prepara- tion, using chloroform instead of phenol improved the average read length from 532 bp to 684 bp. The addition of 2.5% DMSO sequencing PCR reaction resulted in 200 bp longer sequences. Purification using 50 mM EDTA and 0.6 M Sodium acetate(pH 8.0) presented 20 bp longer sequences than that using 50 mM EDTA(pH 8.0) and 0.6 M sodium acetate(pH 5.2). The injection for sequencing analysis using ABI formamide presented 90 bp longer sequences than that of using formamide deionized by resin. Moreover, there were 150 bp more readable sequences in 3.6% PAGE gel than in 4%. Consequently, it was concluded that an average of 700 bp per reaction with 85% accuracy can be obtained by the following optimal conditions: template preparation using chloroform, 2.5% DMSO, 50 mM EDTA and 0.6 M sodium acetate(pH 8.0), ABI formamide and 3.6% gel concentration.

• Fisheries and Aquatic Sciences
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• v.9 no.2
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• pp.70-74
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• 2006

DNA sequence-based typing is considered a robust tool for the discrimination of dinoflagellate species because of the availability of extensive rDNA sequences. Here, we present a rapid, cost-effective DNA-sequencing technique for various PCR products. This sequencing strategy relies on 'nested' or 'tailed' primer labeled with near-infrared dye, and uses a minimal volume of unpurified PCR product (ca. $5{\mu}L$) as the DNA template for sequencing reactions. Reliable and accurate base identification was obtained for several hundred PCR fragments of rRNA genes. This quick, inexpensive technique is widely applicable to sequence-based typing in clinical applications, as well as to large-scale DNA sequencing of the same genomic regions from related species for studies of molecular evolution.

• Environmental Mutagens and Carcinogens
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• v.26 no.1
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• pp.25-29
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• 2006

Antimicrobial peptides (AMPs) play an important role in this response by rapidly killing invading microorganisms. In this study antimicrobial peptide has been isolated from acidified whole body extract of a bivalve mollusk, the marine mussel (Mytilus coruscus). This peptide purified to homogeneity by gel-filtration and reversed-phase high performance liquid chromatography. The molecular weight was 1464.92 Da, determined by MALDI-TOF Mass spectrometry. In addition to growth inhibition of Escherichia coli D31.

• Journal of KIISE:Computer Systems and Theory
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• v.26 no.2
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• pp.155-163
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• 1999

방송통신 프로토콜은 메시지들의 순서가 바뀌거나 메시지 손실이 발생하는 문제점을 갖는 비신뢰 통신 프로토콜이다. 아메바 시스템에서 사용하는 순차적 기반 방송통신 프로토콜은 신뢰성 있고 전송되는 모든 메시지들의 순서를 일정하게 유지할 수 있는 통신 방법중의 하나이다. 비록 아메바 시스템에서는 순차기의 고장이 발생하였을 경우 선출알고리즘을 사용하여 대처하지만, 대부분의 순차기기반 방송통신 프로토콜들은 단일 노드 고장이라는 한가지 단점을 가지고 있다. 이 단점은 순차기 고장으로 인해 전체 시스템에서의 방송통신을 사용할 수 없는 매우 치명적인 현상이 발생한다. 본 논문에서는 전송되는 모든 메시지들의 순서를 일정하게 유지하면서 순차기의 작업량을 줄이는 고장감내 방송통신 프로토콜 알고리즘을 제안한다. 제안된 알고리즘에서는 다수의 순차기를 사용하고 손실된 메시지를 재전송하기 위한 논리적 노드인 기록노드를 사용한다. 또한 순차기노드의 고장을 검출하기 위하여 순차기들간의 논리적 리스트를 구성한다. 제안된 알고리즘의 성능을 측정하기 위하여 시뮬레이션 하였고 실제 유닉스를 기반으로 하는 랜에서 실험했다. 시뮬레이션결과, 제안된 알고리즘은 기존의 순차기기반 알고리즘보다 빠르고, 고장감내 성격을 갖고 있음을 알 수있다.

• Journal of Life Science
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• v.2 no.2
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• pp.108-119
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• 1992

근년에 개발된 효소적인 DNA증폭법을 이용하면 일차구조상의 단편적인 정보만 알면 단 수시간내 해석에 필요한 양의 DNA가 증폭되어 cDNA의 염기배열결정의 신속화, 간편화가 가능하게 되었다. 그러므로 유전자증폭법으로써 PCR법에 관해 기술한다. 그리고 리보좀 RNA는 분자시계로서 생물의 계통을 논하는 데에 있어서는 최적의 조건을 갖춘 고분자화합물이다. 이에 PCR법을 이용한 16S like 리보좀DNA의 증폭법을 다루고, PCR증폭산물의 염기배열결정법에 대해 서술한다. 또한 인위적인 leading error 등을 배제하고 신속한 자동해독과 시간적인 절약이 자동 DNA sequencer의 개발과 시판으로 가능하게 되어 cDNA의 형광색소표식 염기배열결정법에 대해서도 서술한다.