• 한국양식학회지
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• 제10권4호
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• pp.403-407
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• 1997

Intraspecific sequence variation was detected by polymerase chain reaction (PCR) and direct sequencing of a 350-nucleotide region of the mitochondrial 12S rRNA gene of two natural populations (Han River and Nakdong River) and one hatchery stock (Jinhae Inland Fisheries Institute) of local strain common carp, one Israeli strain of common carp stock from Pukyong National University (PKU), and one hybrid between Israeli strain of common carp female and local strain common carp male from PKU stock. There is little variation in 350 bases of the mitochondrial 12S rRNA gene sequences among 2 natural and 1 hatchery local strain common carp populatins, representing abut 7 to 20 nucleotide differences (less than 6%). The sequence of specimens from Han River was more similar to that from Nakdong River (identity=98.0%) than to that from Jinhae Inland Fisheries Institute (identity=96.3%). Sequence variation between Israeli strain and wild local strain common carp was higher than the variation within natural stocks. The level of variation was ranged from 15.7 to 17.7%. The hybrid showed very similar nucleotide4 sequence of 12S rRNA gene to the sequence of Israeli strain with the identity of 98.9%.

• 생명과학회지
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• 제14권6호
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• pp.963-966
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• 2004

능이버섯의 16S ribosomal DNA일부분, IT1, 5.85 ribosomal DNA, ITS2의 전부분, 28S ribosomal DNA 일부분이 포함된 ITS영역의 염기서열을 분석하였다. 이 부분은 716개의 염기쌍으로 구성되었다. 이를 Sarcadon속에 속하는 종들과 비교분석한 결과 같은 능이버섯의 ITS에 관한 다른 분석결과 염기치환 및 결실을 근거로 하였을 경우 $1.8\%$의 차이를 나타내었다. 또한 S. imbricatus와는 $1.8\%$, S. sequamous와는 $10\%$차이를 나타내었다. 이는 능이버섯이 숙주, 서식지 환경 등의 특수성 때문에 자연상태에서 유전자교류가 일어나지 않기 때문인 것으로 생각된다.

• 대한전자공학회논문지SP
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• 제48권5호
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• pp.55-62
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• 2011

본 논문은 조도 변화에 의해 동영상 시퀀스에서 발생하는 주기적인 색상 및 밝기 변화를 저감하기 위한 알고리즘을 제안한다. 밝기 변화가 주기적으로 일어나는 광원 환경에서 동영상을 촬영하는 경우, 노출 시간이 짧아짐에 따라 색상 및 밝기가 주기적으로 변하는 색 유동성 현상, 즉 컬러 롤링 현상(color rolling phenomenon)이 발생한다. 색 유동성 현상이 발생하는 경우 화이트 밸런스 알고리즘을 적용함으로써 개별 프레임의 색상을 보정할 수 있지만, 프레임 간에 발생하는 밝기 차이를 고려하지 않기 때문에 동영상의 밝기가 주기적으로 변하는 문제가 남게 된다. 제안하는 방법에서는 프레임 간에 발생하는 밝기 변화를 추정함으로써 색 유동성 현상으로 인해 나타나는 밝기 및 색상 변화를 보정하는 알고리즘을 제안한다. 이 때, 움직이는 물체에 의해 발생하는 밝기 변화 추정 에러를 줄이기 위해 움직임 검출(motion detection) 방법에 기반하여 색 유동성에 의한 밝기 변화만을 선택적으로 검출하여 보상한다. 또한 이전 밝기 정보를 이용하여 색 유동성에 의한 밝기 변화량을 예측함으로써 실시간 영상 처리에 적합하도록 하였다. 실험 결과를 통해 제안하는 방법을 사용하는 경우 조도 변화에 의한 밝기 변화를 보상함으로써 동영상의 프레임 간 밝기 및 색상의 항상성을 유지하는 것을 확인할 수 있다.

• Korean Journal of Mathematics
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• 제15권1호
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• pp.13-26
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• 2007

We prove that every strong null sequence in a Banach space X lies inside the range of a vector measure of bounded variation if and only if the condition $\mathcal{N}_1(X,{\ell}_1)={\Pi}_1(X,{\ell}_1)$ holds. We also prove that for $1{\leq}p<{\infty}$ every strong ${\ell}_p$ sequence in a Banach space X lies inside the range of an X-valued measure of bounded variation if and only if the identity operator of the dual Banach space $X^*$ is ($p^{\prime}$,1)-summing, where $p^{\prime}$ is the conjugate exponent of $p$. Finally we prove that a Banach space X has the property that any sequence lying in the range of an X-valued measure actually lies in the range of a vector measure of bounded variation if and only if the condition ${\Pi}_1(X,{\ell}_1)={\Pi}_2(X,{\ell}_1)$ holds.

• 전기학회논문지
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• 제64권9호
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• pp.1315-1322
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• 2015

This paper proposes an active islanding detection method for the BESS (Battery Energy Storage System) with 3-phase inverter which is connected to the AC grid. The proposed method adopts the DDSRF (Decoupled Double Synchronous Reference Frame) PLL (Phase Locked-Loop) so that the independent control of positive-sequence and negative-sequence current is successfully carried out using the detected phase angle information. The islanding state can be detected by sensing the variation of negative-sequence voltage at the PCC (Point of Common Connection) due to the injection of 2-3% negative-sequence current from the BESS. The proposed method provides a secure and rapid detection under the variation of negative-sequence voltage due to the sag and swell. The feasibility of proposed method was verified by computer simulations with PSCAD/EMTDC and experimental analyses with 5kW hardware prototype for the benchmark circuit of islanding detection suggested by IEEE 1547 and UL1741. The proposed method would be applicable for the secure detection of islanding state in the grid-tied Microgrid.

• Animal cells and systems
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• 제4권3호
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• pp.267-272
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• 2000

Partial sequence of the mitochondrial cytochrome b gene was analyzed to investigate genetic variation from 10 geographic populations of Granulilittorina exigua in Korea. The sequence of 282 base pairs was determined by PCR-directed silver sequencing method. The sequences of two species within the genus Littorina reserved in NIH blast search were utilized to determine geographic variations of species referred. The levels of mtDNA sequence differences were 0.00-2.54% within populations and 0.71-4.43% between populations. There were four amino acid differences between representative species of the genera Granulilittorina and Littorina, but no differences within populations of the genus Granulilittorina. The UPGMA and the N-J trees based on Tamura-Nei genetic distance matrix were constructed, which showed that the genus Granulilittorina was divided into three groups such as eastern (even exception for Tokdo population), southern, and western regional populations. The degrees of genetic divergence within populations of each group were p=0.021, p=0.019, and p=0.018, respectively. The divergence between the eastern and southern populations was p=0.032, showing closer relationship than with the western populations (p=0.052). Based on the diverged time estimation, the eastern and southern populations of Granulilittorina exigua in Korea diverged from the western populations about 2.1 MYBP, and the eastern and southern populations diverged from each other about 1.3 MYBP.

• Journal of Microbiology
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• 제33권3호
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• pp.252-259
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• 1995

The PCR was employed to detect and characterize the bovine immunodeficiency-like virus (BIV), which is a newly recognized member of the I entivirinae of the retroviruses. Degenerate primers representing the conserved regions in the pol genes of the Lentivirinae, were used to detect proviral DNA obtained from the bovine embryonic spleen cell cultures infected with BIV. The PCR amplified DNA fragment was molecularly cloned and sequenced. The BIV DNA fragment contained a sequence identical to that reported by Garvey et al. (Garvey et al., 1990. Virology, 175, 391-409). With the degenerate primers, peripheral blood mononuclear cells (PBMCs) of sick cattle and cells cultured with BIV were tested to determine genetic variation of BIV pol conserved sequence. We found the sequence heterogeneity within cultures and most variations occurred at the third base of codons that would not lead to amino acid substitutions. Another change was GAG (Glu) to AAG (Lys) within the BIV isolates. Interestingly, the altered sequence is also found in other lentiviruses such as HIV-2, SIV mac, CAEV and EIAV.

• 대한전자공학회:학술대회논문집
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• 대한전자공학회 2001년도 제14회 신호처리 합동 학술대회 논문집
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• pp.355-358
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• 2001

본 논문에서는 객체의 움직임에 의한 모양 변화를 표현하기 위하여 MPEG-7 에 제안된 모양 시퀀스 기술자(Shape Sequence Descriptor)에 대하여 설명하고, 모양 시퀀스 기술자 추출에 필요한 Shape Variation Map 생성을 위한 두 가지 방법을 비교하였다. 기존의 방법은 물체의 평균 적인 모양에 가중치를 두어 생성되며, 새로운 방법은 물체의 움직임에 의해 변화되는 부분에 더 가중치를 두는 방법으로 생성된다. 또한 최종적으로 사용되는 특징 값으로 Zernike moment를 이용하는 방법과 ART(Angular Radial Transform)을 이용하는 방법을 비교하여 모양 시퀀스 검색을 위한 가장 효율적인 방법을 제안하였다.

• Parasites, Hosts and Diseases
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• 제53권3호
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• pp.253-258
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• 2015

Toxoplasma gondii, an obligate intracellular protozoan parasite of the phylum Apicomplexa, can infect all warm-blooded vertebrates, including humans, livestock, and marine mammals. The aim of this study was to investigate whether superoxide dismutase (SOD) of T. gondii can be used as a new marker for genetic study or a potential vaccine candidate. The partial genome region of the SOD gene was amplified and sequenced from 10 different T. gondii isolates from different parts of the world, and all the sequences were examined by PCR-RFLP, sequence analysis, and phylogenetic reconstruction. The results showed that partial SOD gene sequences ranged from 1,702 bp to 1,712 bp and A + T contents varied from 50.1% to 51.1% among all examined isolates. Sequence alignment analysis identified total 43 variable nucleotide positions, and these results showed that 97.5% sequence similarity of SOD gene among all examined isolates. Phylogenetic analysis revealed that these SOD sequences were not an effective molecular marker for differential identification of T. gondii strains. The research demonstrated existence of low sequence variation in the SOD gene among T. gondii strains of different genotypes from different hosts and geographical regions.

• Genomics & Informatics
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• 제6권4호
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• pp.166-172
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• 2008

A multitude of protein-coding sequence variations (CVs) in the human genome have been revealed as a result of major initiatives, including the Human Variome Project, the 1000 Genomes Project, and the International Cancer Genome Consortium. This naturally has led to debate over how to accurately assess the functional consequences of CVs, because predicting the functional effects of CVs and their relevance to disease phenotypes is becoming increasingly important. This article surveys and compares variation databases and in silico prediction programs that assess the effects of CVs on protein function. We also introduce a combinatorial approach that uses machine learning algorithms to improve prediction performance.