• Journal of Aquaculture
• /
• v.10 no.4
• /
• pp.403-407
• /
• 1997

Intraspecific sequence variation was detected by polymerase chain reaction (PCR) and direct sequencing of a 350-nucleotide region of the mitochondrial 12S rRNA gene of two natural populations (Han River and Nakdong River) and one hatchery stock (Jinhae Inland Fisheries Institute) of local strain common carp, one Israeli strain of common carp stock from Pukyong National University (PKU), and one hybrid between Israeli strain of common carp female and local strain common carp male from PKU stock. There is little variation in 350 bases of the mitochondrial 12S rRNA gene sequences among 2 natural and 1 hatchery local strain common carp populatins, representing abut 7 to 20 nucleotide differences (less than 6%). The sequence of specimens from Han River was more similar to that from Nakdong River (identity=98.0%) than to that from Jinhae Inland Fisheries Institute (identity=96.3%). Sequence variation between Israeli strain and wild local strain common carp was higher than the variation within natural stocks. The level of variation was ranged from 15.7 to 17.7%. The hybrid showed very similar nucleotide4 sequence of 12S rRNA gene to the sequence of Israeli strain with the identity of 98.9%.

• Journal of Life Science
• /
• v.14 no.6 s.67
• /
• pp.963-966
• /
• 2004

The sequence of ITS (partial 16S ribosomal DNA, complete ITS1, 5.8S ribosomal DNA and ITS2, and partial 28S ribosomal DNA) was analysed by PCR and autosequencing in Sarcodon aspratus. The ITS lenght of S. aspratus was 716 base pair. As this sequence compared with other reports on S. aspratus (ace No AF335110), the sequence variation based on nucleotide deletion and substitution was $1.8\%$. This nucleotide variation rate in same species was very higher than in other species. Also, the sequence varitation rates between this S. aspratus and S. imbricatus, and S. squamus were $8\%\;and\;10\%$, respectively. This results suggested that the high sequence variation of S. aspratus might be caused specific host and inhabitat environment which limited gene flow.

• Journal of the Institute of Electronics Engineers of Korea SP
• /
• v.48 no.5
• /
• pp.55-62
• /
• 2011

In this paper, a temporal color and luminance variation suppression algorithm for a digital video sequence is proposed by considering time-varying light source. When a video sequence is sampled with the periodically emitting illuminant and with a short exposure time, the color rolling phenomenon occurs, where the color and the luminance of the image periodically change from field to field. In conventional signal processing techniques, the luminance variation remaining in the resultant video sequence degrades the constancy of the image sequence. In the proposed method, we obtain video sequences with constant luminance and color by compensating for the inter-field luminance variation. Based on a motion detection technique, the amount of the luminance variation for each channel is estimated on the background of the sequence without the effects of moving objects. The experimental results clearly show that our strategy efficiently estimated the illuminant change without being affected by moving objects, and the variations were efficiently reduced.

• Korean Journal of Mathematics
• /
• v.15 no.1
• /
• pp.13-26
• /
• 2007

We prove that every strong null sequence in a Banach space X lies inside the range of a vector measure of bounded variation if and only if the condition $\mathcal{N}_1(X,{\ell}_1)={\Pi}_1(X,{\ell}_1)$ holds. We also prove that for $1{\leq}p<{\infty}$ every strong ${\ell}_p$ sequence in a Banach space X lies inside the range of an X-valued measure of bounded variation if and only if the identity operator of the dual Banach space $X^*$ is ($p^{\prime}$,1)-summing, where $p^{\prime}$ is the conjugate exponent of $p$. Finally we prove that a Banach space X has the property that any sequence lying in the range of an X-valued measure actually lies in the range of a vector measure of bounded variation if and only if the condition ${\Pi}_1(X,{\ell}_1)={\Pi}_2(X,{\ell}_1)$ holds.

• The Transactions of The Korean Institute of Electrical Engineers
• /
• v.64 no.9
• /
• pp.1315-1322
• /
• 2015

This paper proposes an active islanding detection method for the BESS (Battery Energy Storage System) with 3-phase inverter which is connected to the AC grid. The proposed method adopts the DDSRF (Decoupled Double Synchronous Reference Frame) PLL (Phase Locked-Loop) so that the independent control of positive-sequence and negative-sequence current is successfully carried out using the detected phase angle information. The islanding state can be detected by sensing the variation of negative-sequence voltage at the PCC (Point of Common Connection) due to the injection of 2-3% negative-sequence current from the BESS. The proposed method provides a secure and rapid detection under the variation of negative-sequence voltage due to the sag and swell. The feasibility of proposed method was verified by computer simulations with PSCAD/EMTDC and experimental analyses with 5kW hardware prototype for the benchmark circuit of islanding detection suggested by IEEE 1547 and UL1741. The proposed method would be applicable for the secure detection of islanding state in the grid-tied Microgrid.

• Animal cells and systems
• /
• v.4 no.3
• /
• pp.267-272
• /
• 2000

Partial sequence of the mitochondrial cytochrome b gene was analyzed to investigate genetic variation from 10 geographic populations of Granulilittorina exigua in Korea. The sequence of 282 base pairs was determined by PCR-directed silver sequencing method. The sequences of two species within the genus Littorina reserved in NIH blast search were utilized to determine geographic variations of species referred. The levels of mtDNA sequence differences were 0.00-2.54% within populations and 0.71-4.43% between populations. There were four amino acid differences between representative species of the genera Granulilittorina and Littorina, but no differences within populations of the genus Granulilittorina. The UPGMA and the N-J trees based on Tamura-Nei genetic distance matrix were constructed, which showed that the genus Granulilittorina was divided into three groups such as eastern (even exception for Tokdo population), southern, and western regional populations. The degrees of genetic divergence within populations of each group were p=0.021, p=0.019, and p=0.018, respectively. The divergence between the eastern and southern populations was p=0.032, showing closer relationship than with the western populations (p=0.052). Based on the diverged time estimation, the eastern and southern populations of Granulilittorina exigua in Korea diverged from the western populations about 2.1 MYBP, and the eastern and southern populations diverged from each other about 1.3 MYBP.

• Journal of Microbiology
• /
• v.33 no.3
• /
• pp.252-259
• /
• 1995

The PCR was employed to detect and characterize the bovine immunodeficiency-like virus (BIV), which is a newly recognized member of the I entivirinae of the retroviruses. Degenerate primers representing the conserved regions in the pol genes of the Lentivirinae, were used to detect proviral DNA obtained from the bovine embryonic spleen cell cultures infected with BIV. The PCR amplified DNA fragment was molecularly cloned and sequenced. The BIV DNA fragment contained a sequence identical to that reported by Garvey et al. (Garvey et al., 1990. Virology, 175, 391-409). With the degenerate primers, peripheral blood mononuclear cells (PBMCs) of sick cattle and cells cultured with BIV were tested to determine genetic variation of BIV pol conserved sequence. We found the sequence heterogeneity within cultures and most variations occurred at the third base of codons that would not lead to amino acid substitutions. Another change was GAG (Glu) to AAG (Lys) within the BIV isolates. Interestingly, the altered sequence is also found in other lentiviruses such as HIV-2, SIV mac, CAEV and EIAV.

• Proceedings of the IEEK Conference
• /
• 2001.09a
• /
• pp.355-358
• /
• 2001

본 논문에서는 객체의 움직임에 의한 모양 변화를 표현하기 위하여 MPEG-7 에 제안된 모양 시퀀스 기술자(Shape Sequence Descriptor)에 대하여 설명하고, 모양 시퀀스 기술자 추출에 필요한 Shape Variation Map 생성을 위한 두 가지 방법을 비교하였다. 기존의 방법은 물체의 평균 적인 모양에 가중치를 두어 생성되며, 새로운 방법은 물체의 움직임에 의해 변화되는 부분에 더 가중치를 두는 방법으로 생성된다. 또한 최종적으로 사용되는 특징 값으로 Zernike moment를 이용하는 방법과 ART(Angular Radial Transform)을 이용하는 방법을 비교하여 모양 시퀀스 검색을 위한 가장 효율적인 방법을 제안하였다.

• Parasites, Hosts and Diseases
• /
• v.53 no.3
• /
• pp.253-258
• /
• 2015

Toxoplasma gondii, an obligate intracellular protozoan parasite of the phylum Apicomplexa, can infect all warm-blooded vertebrates, including humans, livestock, and marine mammals. The aim of this study was to investigate whether superoxide dismutase (SOD) of T. gondii can be used as a new marker for genetic study or a potential vaccine candidate. The partial genome region of the SOD gene was amplified and sequenced from 10 different T. gondii isolates from different parts of the world, and all the sequences were examined by PCR-RFLP, sequence analysis, and phylogenetic reconstruction. The results showed that partial SOD gene sequences ranged from 1,702 bp to 1,712 bp and A + T contents varied from 50.1% to 51.1% among all examined isolates. Sequence alignment analysis identified total 43 variable nucleotide positions, and these results showed that 97.5% sequence similarity of SOD gene among all examined isolates. Phylogenetic analysis revealed that these SOD sequences were not an effective molecular marker for differential identification of T. gondii strains. The research demonstrated existence of low sequence variation in the SOD gene among T. gondii strains of different genotypes from different hosts and geographical regions.

• Genomics & Informatics
• /
• v.6 no.4
• /
• pp.166-172
• /
• 2008

A multitude of protein-coding sequence variations (CVs) in the human genome have been revealed as a result of major initiatives, including the Human Variome Project, the 1000 Genomes Project, and the International Cancer Genome Consortium. This naturally has led to debate over how to accurately assess the functional consequences of CVs, because predicting the functional effects of CVs and their relevance to disease phenotypes is becoming increasingly important. This article surveys and compares variation databases and in silico prediction programs that assess the effects of CVs on protein function. We also introduce a combinatorial approach that uses machine learning algorithms to improve prediction performance.