• Journal of Microbiology and Biotechnology
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• 제17권2호
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• pp.335-341
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• 2007

For the development of qualitative and quantitative PCR methods of genetically modified (GM) pepper developed in Korea, a capsanthin-capsorubin synthase (CCS) gene was used as the endogenous reference gene. The primer pair ccs-F/R amplifying the pepper endogenous gene gave rise to an amplicon of 102 bp. No amplified product was observed when DNA samples from 16 different plants were used as templates. The construct-specific primer pairs amplifying the junction region of the bar gene and Ti7 introduced in GM pepper gave rise to an amplicon of 182 bp. Quantitative PCR assay was performed using a TaqMan probe and a standard plasmid as a reference molecule, which contained both an endogenous and event-specific sequence. For the validation of this method, the test samples containing 0.1, 1, 3, 5, and 10% GM pepper were quantified.

• Journal of information and communication convergence engineering
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• 제11권2호
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• pp.124-131
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• 2013

Extraction and representation of postures and/or gestures from human activities in videos have been a focus of research in this area of action recognition. With various applications cropping up from different fields, this paper seeks to improve the performance of these action recognition machines by proposing a shape-based silhouette-edge descriptor for the human body. Information entropy, a method to measure the randomness of a sequence of symbols, is used to aid the selection of vital key postures from video frames. Morphological operations are applied to extract and stack edges to uniquely represent different actions shape-wise. To classify an action from a new input video, a Hausdorff distance measure is applied between the gallery representations and the query images formed from the proposed procedure. The method is tested on known public databases for its validation. An effective method of human action annotation and description has been effectively achieved.

• 한국정보과학회:학술대회논문집
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• 한국정보과학회 2007년도 가을 학술발표논문집 Vol.34 No.2 (B)
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• pp.22-27
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• 2007

Phosphorylation is one of the most important post translational modifications which regulate the activity of proteins. The problem of predicting phosphorylation sites is the first step of understanding various biological processes that initiate the actual function of proteins in each signaling pathway. Although many prediction methods using single or multiple features extracted from protein sequences have been proposed, systematic data integration approach has not been applied in order to improve the accuracy of predicting general phosphorylation sites. In this paper, we propose an optimal way of integrating multiple features in the framework of multiple kernel learning. We optimally combine seven kernels extracted from sequence, physico-chemical properties, pairwise alignment, and structural information. Using the data set of Phospho. ELM, the accuracy evaluated by 5-fold cross-validation reaches 85% for serine, 85% for threonine, and 81% for tyrosine. Our computational experiments show significant improvement in the performance of prediction relative to a single feature, or to the combined feature with equal weights. Moreover, our systematic integration method significantly improves the prediction preformance compared with the previous well-known methods.

• 대한용접접합학회:학술대회논문집
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• 대한용접접합학회 2009년 추계학술발표대회
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• pp.102-102
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• 2009

The purpose of this study is to establish the predictive equation of transversal residual stress at the thick weldment of large container ship. In order to do it, the variables used for this study were restraint degree, yield strength of base material, thickness of weldment and welding heat input. Here, the level of restraint degree at the thick weldment of container ship having the various welding sequence was calculated using FEA. From the result, the h-type specimen was designed to simulate the level of restraint degree at the actual weldment of containership. With H-type test specimen designed, the effect of the variables on the distribution of transversal residual stress at the weldment in a container ship was evaluated using the comprehensive FEA. Based on the results, the predictive equations of mean value and the distribution of transverse residual stress in each location of residual stress were established using dimensional analysis and multiple-regression method. The validation of predictive equations was verified by comparing with measured results by XRD in the actual weldment of the ship.

• KSII Transactions on Internet and Information Systems (TIIS)
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• 제15권6호
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• pp.1981-1995
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• 2021

When a person tries to conceal emotions, real emotions will manifest themselves in the form of micro-expressions. Research on facial micro-expression recognition is still extremely challenging in the field of pattern recognition. This is because it is difficult to implement the best feature extraction method to cope with micro-expressions with small changes and short duration. Most methods are based on hand-crafted features to extract subtle facial movements. In this study, we introduce a method that incorporates optical flow and deep learning. First, we take out the onset frame and the apex frame from each video sequence. Then, the motion features between these two frames are extracted using the optical flow method. Finally, the features are inputted into an improved MobileNetV2 model, where SVM is applied to classify expressions. In order to evaluate the effectiveness of the method, we conduct experiments on the public spontaneous micro-expression database CASME II. Under the condition of applying the leave-one-subject-out cross-validation method, the recognition accuracy rate reaches 53.01%, and the F-score reaches 0.5231. The results show that the proposed method can significantly improve the micro-expression recognition performance.

• Nuclear Engineering and Technology
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• 제55권7호
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• pp.2438-2446
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• 2023

To cool down a nuclear reactor core and prevent the fuel damage without a pump-driven active component during any anticipated accident, the passive emergency core cooling system (PECCS) was designed and adopted in an advanced light water reactor, i-POWER. In this study, for a validation of the cooling capability of PECCS, thermal-hydraulic integral effect tests were performed with the ATLAS facility by simulating intermediate and small break loss-of-coolant accidents (IBLOCA and SBLOCA). The test result showed that PECCS could effectively depressurize the reactor coolant system by supplying the safety injection water from the safety injection tanks (SITs). The result pointed out that the safety injection from IRWST should have been activated earlier to inhibit the excessive core heat-up. The sequence of the PECCS injection and the major thermal hydraulic transient during the SBLOCA transient was similar to the result of the IBLOCA test with the equivalent PECCS condition. The test data can be used to evaluate the capability of thermal hydraulic safety analysis codes in predicting IBLOCA and SBLOCA transients under an operation of passive safety system.

• Pediatric Infection and Vaccine
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• 제16권1호
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• pp.47-53
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• 2009

목 적: 국내 임신부의 생식기와 직장에서 분리된 B군 연구균의 혈청형 분포를 분자생물학적인 방법으로 확인하고자 하였다. 방 법: 산전 진찰을 위해 고양시 소재 4개의 산부인과병원을 방문한 임신 35주 이상 임신부의 생식기와 직장에서 분리 되어 $-70^{\circ}C$에 보관 중이던 42개의 B군 연구균에서 DNA를 추출하고 혈청형 특이 PCR 과 염기서열 분석을 이용하여 혈청형을 결정하였다. 결 과: 42개 균주 모두에서 혈청형 특이 PCR 과 염기서열 분석을 통해 혈청형을 결정할 수 있었고 빈도순으로 III형이 12균주(28.6%), V형이 11균주(26.2%), Ia형이 11균주(26.2%), VI형이 4균주(9.5%), Ib형이 2균주(4.8%), II형이 2균주(4.8%)였다. 결 론: 국내의 후기 임신부에서 흔하게 분리되는 B군 연구균의 혈청형은 III형, V형, Ia형이었고 본 연구에서 이용한 혈청형 특이 PCR과 염기서열 분석은 앞으로도 관련 연구에 유용하게 사용할 수 있을 것으로 보인다.

• Journal of Yeungnam Medical Science
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• 제24권2호
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• pp.119-128
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• 2007

목적 : 이 연구는 낮은 b값의 SSFP-확산강조영상에 의한 물분자의 확산 성질 측정이 가능한지를 알기 위한 모형연구를 하고 이 기법이 골수질환에 적용이 가능한 가를 아는 것이 목적이다. 재료 및 방법: 모형 연구：순수한 물로 구성된 모형에서 확산강조 영상을 시행하였다. 섭씨 3도, 23도 그리고 63도의 순수한 물로 구성된 모형에서 SSFP 확산강조영상과 echo plannar imaging (EPI) 확산강조영상 (b값: $1000s/mm^2$)을 모두 시행하여 각각에서 신호 대 잡음 비 (SNR; signal to noise ratio)와 확산계수를 얻었다. 임상 연구 : 10명의 천골 부족 골절, 10명의 골다공증에 의한 급성 요추 압박골절, 그리고 전이암에 의한 요추 압박골절 8명에서 각각 SSFP 확산강조영상을 시행하였다. SSFP 확산 강조영상 외 확산계수를 측정하기 위해 single shot stimulated echo-acquisition mode sequence 를 이용한 확산강조영상을 시행하였다. 결과: 모형연구에서 EPI 확산 강조영상뿐만 아니라 SSFP 확산강조영상에서 물의 온도가 증가됨에 따라 신호강도의 감소를 보였다. EPI-확산계수 영상에서 확산계수 값은 3도의 물은 $0.13{\times}10^{-3}mm^2/s$, 23도는 $0.22{\times}10^{-3}mm2/s$ 그리고 63도에서 $0.37{\times}10^{-3}mm^2/s$를 나타냈다. 이러한 결과는 SSFP 기법은 비록 낮은 b 값을 가지지만 확산 강조 영상으로 확신된다. SSFP 확산강조영상에서 모든 천골 부족 골절과 골다공증 척추 압박골절은 높은 확산계수를 의미하는 저신호강도를 전이암에 의한 압박골절은 낮은 확산 값을 나타내는 고신호 강도를 보였다. 결론 : SSFP 확산강조영상에서 다른 확산계수를 가진 골수질환이 영상화 되었으며 모든 양성골절은 주위 정상 골수에 비해 저신호강도, 악성 종양에 의한 골절은 고신호강도로 관찰되었다.

• Journal of Pharmaceutical Investigation
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• 제41권3호
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• pp.191-196
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• 2011

In this study simple and sensitive high performance liquid chromatographic method using a commercially available column, was developed and validated for the determination of zolpidem tartrate in human plasma. The developed method with suitable validation was applied to a bioequivalence study of two different kinds of zolpidem tartrate. Two different formulations containing 10 mg of zolpidem tartate (CAS : 99294-93-6) were compared in 24 healthy male volunteers in order to compare the bioavailability and prove the bioequivalence. The study was performed in an open, single dose randomized, 2-sequence, cross-over design in 24 healthy male volunteers with a one-week washout period. Blood samples for pharmacokinetic profiling were drawn at selected times during 12 h. The mean $AUC_{0-12h}$, $C_{max}$, $T_{max}$ and $T_{1/2}$ were $676.6{\pm}223.4$ $ng{\cdot}h{\cdot}mL^{-1}$, $177.4{\pm}34.2$ $ng{\cdot}mL^{-1}$, and $0.8{\pm}0.4$ and $3.5{\pm}2.1$, respectively, for the test formulations, and $640.7{\pm}186.6$ $ng{\cdot}h{\cdot}mL^{-1}$, $193.0{\pm}64.5$ $ng{\cdot}mL^{-1}$, and $0.9{\pm}0.4$ and $2.7{\pm}0.9$, respectively, for the reference formulation. Both primary target parameters $AUC_{0-12h}$ and $C_{max}$ were log-transformed and tested parametrically by analysis of variance (ANOVA). 90% confidence intervals of $AUC_{0-12h}$ and $C_{max}$ were in the range of acceptable limits of bioequivalence (80-125%). Based on these results, the two formulations of zolpidem tartate are considered to be bioequivalent.

• 한국패류학회지
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• 제30권4호
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• pp.429-431
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• 2014

본 연구를 통하여 연체동물 전용 BLAST 서버 (Version II)가 웹주소 http://www.malacol.or.kr/blast에 구축되었다. 연체동물을 대상으로 하는 연구에 있어 필요한 정보를 매우 빠르게 얻을 수 있었다. 본 시스템을 사용하여 앞으로도 많은 연구가 진행되어지길 바라며, 아울러 많은 연체동물 연구자들에게 많은 도움이 되리라고 사료된다.