• The Korean Journal of Zoology
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• v.31 no.3
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• pp.236-242
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• 1988

Mitochondrial DNAs of two Mdh allelotypes of the dark chub, Z. temmincki inhabiting in Korean fresh water, were analysed. Samples of each type were collected from four populations, and the fragment patterns for mtdNA of each type were explained from 7 of the eleven restriction enzymes with hexanucleotide recognition site. Genome size was approximately 16.7 kilobases. The highly typical mtdNA fragments of each type were discovered in digestion profiles produced by Eco RI and Pst I enzyrnes. The comparisons of restriction fragment patterns and relative digestion maps permitted the estimation of fragment homology (F) and nucleotide sequence divergence(p). Between the two identical types, sequence divergence(p) was 0.128(MS), and 0.045(MM), ; between the two different types, 0.195 (range 0.177-0.226). These result may provide a distinct difference more than the value derived from allozyrne analysis, and a powerful new molecular approach for assessing genetic-evolutionary relationship among fishes.

• The Journal of Korean Society of Virology
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• v.29 no.3
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• pp.175-182
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• 1999

An attractive target for anti-herpes chemotherapy is the herpes simplex virus 1 (HSV-1) protease encoded by the UL26 gene. HSV-1 protease is essential for DNA packaging and virus maturation. To perform high throughput for potent inhibitors, the efficient production of larger amounts of highly purified enzyme and protease activity assay method must be established. In this report, expression in E. coli and purification of the protease gene of HSV-1 strain F was investigated. The protease gene was cloned pET28, and the nucleotide sequence of protease catalytic domain of HSV-1 compared strain F with other strains (KOS and CL101). In these results the F strain was different in base sequence. However, the amino acid sequence was identifical. The HSV-1 protease was purified with His-tagged affinity column. The analysis of HSV-1 protease activity was performed by high performance liquid chromatography.

• Parasites, Hosts and Diseases
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• v.36 no.1
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• pp.47-54
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• 1998

Small subunit ribosomal RNA (SSU rRNA) gene nucleotide sequences of bovine ReiLerin isolates from Korea (KLS and KCB) and japan (JHS) were determined. The genes from each isolate were amplified by the polymerase chain reaction and the approxi- mately 1.8 kb product cloned and sequenced by a modified dideoxynucleotide method. Overlapping gene segments produced with a series of primers were sequenced, resoRting in a complete DNA sequence for both forward and reverse strands of the SSU rRNA genes of each isolate. SSU rRNA gene sequences (termed Type A) were identical among the bovine ReiLeri,n isolates from Korea and the isolate from Japan. A GenBank data library homolo- gy search showed the sequence to be the same as that listed as leiLeyia buKeLi isolated from cattle in Marula, Kenya.

• Mycobiology
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• v.47 no.2
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• pp.200-206
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• 2019

Allelic differences in A and B mating-type loci are a prerequisite for the progression of mating in the genus Pleurotus eryngii; thus, the crossing is hampered by this biological barrier in inbreeding. Molecular markers linked to mating types of P. eryngii KNR2312 were investigated with randomly amplified polymorphic DNA to enhance crossing efficiency. An A4-linked sequence was identified and used to find the adjacent genomic region with the entire motif of the A locus from a contig sequenced by PacBio. The sequence-characterized amplified region marker $7-2_{299}$ distinguished A4 mating-type monokaryons from KNR2312 and other strains. A BLAST search of flanked sequences revealed that the A4 locus had a general feature consisting of the putative HD1 and HD2 genes. Both putative HD transcription factors contain a homeodomain sequence and a nuclear localization sequence; however, valid dimerization motifs were found only in the HD1 protein. The ACAAT motif, which was reported to have relevance to sex determination, was found in the intergenic region. The SCAR marker could be applicable in the classification of mating types in the P. eryngii breeding program, and the A4 locus could be the basis for a multi-allele detection marker.

• The Bulletin of The Korean Astronomical Society
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• v.39 no.2
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• pp.48.1-48.1
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• 2014

We investigate the evolution of galaxies in mid-infrared (MIR) $[3.4{\mu}m]-[12{\mu}m]$ color versus $12{\mu}$ luminosity diagram using Wide-field Infrared Survey Explorer data for member galaxies of the A2199 supercluster at $z{\simeq}0.03$. In the MIR color-luminosity diagram, we classify galaxies into three MIR classes: MIR blue cloud (massive, quiescent and mostly early-type), MIR star-forming sequence (mostly late-type), and MIR green valley galaxies. Both MIR green valley galaxies and MIR blue cloud galaxies are optically red sequence populations, and there is no significant difference in star formation rates and stellar masses between them. We compare cumulative distribution functions of surface galaxy number density and of cluster/group-centric distance between three MIR classes. However, when considering only early-type galaxies, the difference between MIR blue cloud galaxies and MIR green valley galaxies disappears. In contrast, the intermediate trend of MIR green valley galaxies is still found for late-type galaxies. We discuss our results concerning the difference of evolution between early- and late-type galaxies and the connection to environment.

• Korean Journal of Breeding Science
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• v.43 no.4
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• pp.273-281
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• 2011

Fusarium head blight (FHB), also known as scab, caused mainly by Fusarium graminearum is a devastating disease of wheat in regions that are warm and humid during flowering. In addition to significant yield and quality losses, the mycotoxin deoxynivalenol produced by the pathogen in infected wheat kernels is a serious problem for food and feed safety. Twenty- three Korean cultivars and "Sumai 3", which is a FHB-resistant Chinese cultivar were tested for Type I, Type II resistances of FHB. Three cultivars were identified as resistant in Type I assessment, and two cultivars were resistant in Type II assessment. Genetic variation and relationship among the cultivars were evaluated on the basis of 11 Simple Sequence Repeat (SSR) and 29 Sequence Tagged Site (STS) markers that were linked to FHB resistance Quantitative Trait Loci (QTL) on chromosome 3BS. One SSR and 7 STS markers detected polymorphisms. Especially, using a STS marker (XSTS3B-57), 32.4% of the variation for Type II FHB resistance could be explained. Genetic relationship among Korean wheat cultivars was generally consistent with their released year. These markers on chromosome 3BS have the potential for accelerating the development of Korean wheat cultivars with improved Fusarium head blight resistance through the use of marker-assisted selection.

• Proceedings of the Korean Society of Plant Pathology Conference
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• 2003.10a
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• pp.143.1-143
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• 2003

Hop stunt viroid(HSVd) is a plant pathogen which infect a number of hosts such as grapevine, Citrus and Prunus plants. Sequence variants of HSVd have been divided into three types(i. grapevine and hop, ii. citrus, iii. plum, peach, apricot and almond). Purified RNAs from plum trees were used for the synthesis of cDNA with reverse transcription and amplified by polymerase chain reaction. Cloned cDNAs were sequenced and two different consensus sequence variants were detected. A neighbor-joining analysis was carried out on the sequence variants together with 62 previously described variants of HSVd from hop, plum and other species. Sequence variants from plum trees cultivated in Korea were clustered in HSVd-plum subtype and not in HSVd-hop subtype which were two Korean isolates belongs. These relationship between sequence variants from plum and two Korean isolates in HSVd-hop type supports the other origin for hop stunt disease.

• Management Science and Financial Engineering
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• v.19 no.1
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• pp.29-36
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• 2013

This paper considers common due-date assignment and scheduling on parallel machines. The main decisions are: (a) deter-mining the common due-date; (b) allocating jobs to machines; and (c) sequencing the jobs assigned to each machine. The objective is to minimize the sum of the penalties associated with common due-date assignment, earliness and tardiness. As an extension of the existing studies on the problem, we consider sequence-dependent setup times that depend on the type of job just completed and on the job to be processed. The sequence-dependent setups, commonly found in various manufacturing systems, make the problem much more complicated. To represent the problem more clearly, a mixed integer programming model is suggested, and due to the complexity of the problem, two heuristics, one with individual sequence-dependent setup times and the other with aggregated sequence-dependent setup times, are suggested after analyzing the characteristics of the problem. Computational experiments were done on a number of test instances and the results are reported.

• The Journal of Korean Institute of Communications and Information Sciences
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• v.34 no.1C
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• pp.113-119
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• 2009

The training sequence with the appropriate length for equalization and initial synchronization is necessary before sending the pure data in the burst transmission type DS-UWB system. The length of the training sequence is one of the factors which make throughput decreased. The noble structure with the variable length of the training sequence whose length can be adaptively tailored according to the channel conditions (CM1,CM2,CM3,CM4) in the DS-USB systems is proposed. This structure can increase the throughput without sacrificing the performance than the method with fixed length of training sequence considering the worst case channel conditions. Simulation results under IEEE 802.15.3a channel model show that the proposed scheme can achieve higher throughput than a conventional one with the slight loss of BER performance. And this structure can reduce the computation complexity and power consumption with selecting the short length of the training sequence.

• ETRI Journal
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• v.40 no.6
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• pp.699-713
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• 2018

Orthogonal frequency division multiplexing (OFDM) has been the overwhelmingly prevalent choice for high-data-rate systems due to its superior advantages compared with other modulation techniques. In contrast, a high peak-to-average-power ratio (PAPR) is considered the fundamental obstacle in OFDM systems since it drives the system to suffer from in-band distortion and out-of-band radiation. The partial transmit sequence (PTS) technique is viewed as one of several strategies that have been suggested to diminish the high PAPR trend. The PTS relies upon dividing an input data sequence into a number of subblocks. Hence, three common types of the subblock segmentation methods have been adopted - interleaving (IL-PTS), adjacent (Ad-PTS), and pseudorandom (PR-PTS). In this study, a new type of subblock division scheme is proposed to improve the PAPR reduction capacity with a low computational complexity. The results indicate that the proposed scheme can enhance the PAPR reduction performance better than the IL-PTS and Ad-PTS schemes. Additionally, the computational complexity of the proposed scheme is lower than that of the PR-PTS and Ad-PTS schemes.