• Title/Summary/Keyword: Sequence Matching

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Development Study of A Precooled Turbojet Engine for Flight Demonstration

  • Sato, Tetsuya;Taguchi, Hideyuki;Kobayashi, Hiroaiki;Kojima, Takayuki;Fukiba, Katsuyoshi;Masaki, Daisaku;Okai, Keiichi;Fujita, Kazuhisa;Hongoh, Motoyuki;Sawai, Shujiro
    • Proceedings of the Korean Society of Propulsion Engineers Conference
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    • 2008.03a
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    • pp.109-114
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    • 2008
  • This paper presents the development status of a subscale precooled turbojet engine "S-engine" for the hypersonic cruiser and space place. S-engine employs the precooled-cycle using liquid hydrogen as fuel and coolant. It has $23cm{\times}23cm$ of rectangular cross section, 2.6 m of the overall length and about 100 kg of the target weight employing composite materials for a variable-geometry rectangular air-intake and nozzle. The design thrust and specific impulse at sea-level-static(SLS) are 1.2 kN and 2,000 sec respectively. After the system design and component tests, a prototype engine made of metal was manufactured and provided for the system firing test using gaseous hydrogen in March 2007. The core engine performance could be verified in this test. The second firing test using liquid hydrogen was conducted in October 2007. The engine, fuel supplying system and control system for the next flight test were used in this test. We verified the engine start-up sequence, compressor-turbine matching and performance of system and components. A flight test of S-engine is to be conducted by the Balloon-based Operation Vehicle(BOV) at Taiki town in Hokkaido in October 2008. The vehicle is about 5 m in length, 0.55 m in diameter and 500 kg in weight. The vehicle is dropped from an altitude of 40 km by a high-altitude observation balloon. After 40 second free-fall, the vehicle pulls up and S-engine operates for 60 seconds up to Mach 2. High altitude tests of the engine components corresponding to the BOV flight condition are also conducted.

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Analysis of Genetic Diversity in Cymbidium Varieties Using SRAP (SRAP을 이용한 국내육성 심비디움 품종의 유전적 다양성 분석)

  • Park, Pue Hee;Kim, Mi Seon;Lee, Young Ran;Park, Pil Man;Lee, Dong Soo;Yae, Byeong Woo
    • Korean Journal of Breeding Science
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    • v.43 no.5
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    • pp.399-404
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    • 2011
  • Genetic diversity among 28 Cymbidium varieties was evaluated by using a sequence-related amplified polymorphism (SRAP) marker system. The SRAP marker which was based on the open reading frames (ORFs) regions was developed primarily for Brassica species, but has been applied to various crops. A total of 30 SRAP primer combinations were initially screened. Twenty-eight SRAP primer combinations showed high polymorphism among the 28 Cymbidium varieties, which were consisted of breeding varieties and their parents in National Institute of Horticultural & Herbal Science (NIHHS). The amplified DNA fragments were separated by denaturing acrylamide gels and detected silver staining method. One hundred ninety six polymorphic bands (7 per primer) were generated and ranged from 0.3 to 1.0 kb in size. Polymorphic fragments were scored for calculating simple matching coefficient of genetic similarity and cluster analysis with multi-variate statistical package (MVSP) 3.1. The mean genetic similarity coefficient value was 0.588. The results showed that the correlation between $F_1$ varieties and their parents was high. These studied SRAP markers will be useful tools for genotype identification, germplasm conservation, genetic relationships in Cymbidium.

Application of simple and massive purification system of dsRNA in vivo for acute toxicity to Daphnia magna

  • CHOI, Wonkyun;LIM, Hye Song;KIM, Jin;RYU, Sung-Min;LEE, Jung Ro
    • Entomological Research
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    • v.48 no.6
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    • pp.533-539
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    • 2018
  • The RNA interference (RNAi) has been considered as an important genetic tool and applied to develop a new living modified (LM) crop trait which is an improvement of nutrient quality or pest management. The RNAi of DvSnf7 has been used for resistance to LM maize and the Western Corn Rootworm which is a major agricultural pest for the US Corn Belt. Most of the environmental risk assessments (ERA) of double strand RNA (dsRNA) have been performed using in vitro transcript products, and not in vivo expressed product. A large amount of dsRNA was required for the acute toxicity assay of water fleas. Therefore development of massive dsRNA purification techniques is critical. Daphnia, a freshwater microcrustacean, is a model organism for studying cellular and molecular mechanism involved in life history traits and ecotoxicology. In this study, we established the massive dsRNA purification method using Escherichia coli and implemented acute toxicity assays to Daphnia magna. As a result, the present RNase A and DNase I, dsRNA was efficiently purified without any special techniques or equipment. Even though purified dsRNA existed during the acute toxicity test, lethality or abnormal behavior were not observed in D. magna. These results indicated that GFP and DvSnf7 dsRNA were not significantly affected to D. magna due to their lack of sequence matching in its genome. The purification method of dsRNA and the acute toxicity assay of water fleas using purified dsRNA would be suitable for the toxicological studies of LMOs to aquatic non-target organisms.

Availability of MADLDI-TOF MS for Identification of Gram Positive Bacilli Isolated from Blood Culture

  • Choi, Jin-Un;Kim, Sang-Ha;Hwang, Su-Jeong;Yu, Young-Bin;Kim, Sunghyun;Kim, Young-Kwon
    • Biomedical Science Letters
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    • v.24 no.2
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    • pp.108-115
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    • 2018
  • In the present study, results of the identification of Gram-positive bacilli (GPB) were analyzed by using the MALDI-TOF MS technique to score each 2-year blood culture at a university hospital. In addition, 16S rRNA sequence analyses and MALDI-TOF MS results are compared to targeting strains that had been isolated two or more times within the same patient, to evaluate the usefulness of MALDI-TOF MS in GPB identification. According to the cut-off (${\geq}1.7$) criteria, there were 410 (57.5%) reliable strains and 303 (42.5%) non-identified strains among the GPB identification results of 713 strains, using a microflex MALDI Biotyper (Bruker Daltonik GmbH, Bremen, Germany). The isolation appeared most often in the following order: Corynebacterium striatum, Bacillus cereus, Bacillus subtilis, Paenibacillus urinalis, and Listeria monocytogenes. Nearly three-fourths, 66 out of 89 (74.2%) of the strains for Corynebacterium striatum; 44 out of 60 (73.3%) strains for Bacillus cereus; and all (25 out of 25, 100%) Listeria monocytogenes strains were identified by their high scores of 2.0 or higher. Most (293 strains out of 303) non-identified strains were strains isolated only once and not significant as infectious bacilli. A total of 43 out of 50 (86.0%) strains matched and were able to be identified based on the 16 rRNA sequencing comparison results of strains that were isolated twice or more within the same patient and significant as infection bacilli. Non-matching among 5 out of 7 strains was not identified, even with MALDI-TOF MS. In conclusion, GPB can be identified in blood cultures using MALDI-TOF MS. This can be done accurately with ease, rapidly, and at a low cost. It is also thought to be helpful in GPB diagnosis and treatment.

Reliability and Validity of the Behavioral Observation Method for Assessing Low Back Pain in Patients with Spinal Diseases (척추질환자의 요통사정을 위한 통증행위 관찰법의 신뢰도 및 타당도 검정)

  • Yoon, Ho-Soon;Lee, Eun-Ok
    • Journal of muscle and joint health
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    • v.1 no.1
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    • pp.97-115
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    • 1994
  • The purpose of this study was to examine the reliability and validity of the observed behaviors of subjects who suffered from low back pain with spinal diseases, Thirty two low back pain patients admitted on the neurosurgical unit in an army hospital were compared with 30 normal controls belonged to an army unit, by means of matching the age, hight and weight. Observed pain behaviors were developed by the researcher on the bases of literature and patient observation. This tool consists of 18 behaviors seperated into two major groups : mutually exclusive and concomittent behaviors. The mutually exclusive behaviors included coding cathegories for 6. body motions assumed by the subjects during the observation session. These 6 standardized motions consisted of sitting from standing first, and serially tying down, reclining, sitting again, and then standing, 6 steps walking. Concomittent behaviors consisted of 12 observable patterns that can be observed systematically from the face, grimacing, bracing, rubbing, walking with arms fixed, support with hands on sitting or standing, guarded movement, limping, unbalaced weightbearing, stopped movement from tying position to sitting, sighing and graoning. Subjects were videotrecorded as they performed a 6-standardized sequence of motions, simultanously researcher measured the time spent performing each motion and step length. Patients were asked torate their subjective pain score on the 10 mm graphic rating scale ranging from 'no pain' to 'sever pain'. For scoring of the pain behaviors, two trained nursing officiers independently and simutanously viewwd each videorecording and checked subject 'pain behaviors at the observational item checklist. The result of the study are summarized as follows : 1. Reability of the observational tool was a=.845. 2. Spearman's rho and percentage agreement were p=.97 and 81.7 persent respectively, that indicate adequate interrater reability of this tool. 3. The sensitivity rate of the tool was .875 while specificity rate .866 for differentiating patient from the normal. 4. When difference in the objective pain indices between patient group and control were compared, there was significant difference of all indices, such as pain behavior(t=7.71, p=.0001). spent time performing motion(t=14.2, p=.0001), step length (t=-10.72, p=.0001). 5. There were differences in the objective indices the subjective pain subgroups (low, medium, high). Differences in the mean score of objective pain behavior (F=6.376. p=.005) and spent time for moyion(F=4.631, p=.018). But there were no significant differences in the step length among the subgroups(F=.667, p=.521). 6. Highly correlated pain behavior items wiyh subjective pain score were 'stopped movement from lying position to sitting', 'limping', 'support with hands on sitting or standing', 'bracing', 'guarding' and 'walking with arms fixed'. In summary, although some of rho behavior items such as sighing and groaning in this study could not be observed because of videotaped datd, the reliability and validity of the over all observation method were satifactory. Thus, the results of the present study demonstrate rye potetional utility of the tool in assessing objective pain complementing self-reported pain in low back pain patients.

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Soccer Game Analysis I : Extraction of Soccer Players' ground traces using Image Mosaic (축구 경기 분석 I : 영상 모자익을 통한 축구 선수의 운동장 궤적 추출)

  • Kim, Tae-One;Hong, Ki-Sang
    • Journal of the Korean Institute of Telematics and Electronics S
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    • v.36S no.1
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    • pp.51-59
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    • 1999
  • In this paper we propose the technique for tracking players and a ball and for obtaining players' ground traces using image mosaic in general soccer sequences. Here, general soccer sequences mean the case that there is no extreme zoom-in or zoom-out of TV camera. Obtaining player's ground traces requires that the following three main problems be solved. There main problems: (1) ground field extraction (2) player and ball tracking and team indentification (3) player positioning. The region of ground field is extracted on the basis of color information. Players are tracked by template matching and Kalman filtering. Occlusion reasoning between overlapped players in done by color histogram back-projection. To find the location of a player, a ground model is constructed and transformation between the input images and the field model is computed using four or more feature points. But, when feature points extracted are insufficient, image-based mosaic technique is applied. By this image-to-model transformation, the traces of players on the ground model can be determined. We tested our method on real TV soccer sequence and the experimental results are given.

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Prism-based Mesh Culling Method for Effective Continuous Collision Detection (효율적인 연속 충돌감지를 위한 프리즘 기반의 메쉬 컬링 기법)

  • Woo, Byung-Kwang;You, Hyo-Sun;Choi, Yoo-Joo
    • Journal of the Korea Computer Graphics Society
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    • v.15 no.4
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    • pp.1-11
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    • 2009
  • In this paper, we present a prism-based mesh culling method to improve effectiveness of continuous collision detection which is a major bottleneck in a simulation using polygonal mesh models. A prism is defined based on two matching triangles between a sequence of times m a polygonal model. In order to detect potential colliding set(PCS) of prism between two polygonal models in a unit time, we apply the visibility test based on the occlusion query to two sets of prisms which are defined from two polygonal models in a unit time. Moreover, we execute the narrow band culling based on SAT(Separating Axis Test) to define potential colliding prism pairs from PCS of prisms extracted as a result of the visibility test. In the SAT, we examine one axis to be perpendicular to a plane which divides a 3D space into two half spaces to include each prism. In the experiments, we applied the proposed culling method to pairs of polygonal models with the different size and compared the number of potential colliding prism pairs with the number of all possible prism pairs of two polygonal models. We also compared effectiveness and performance of the visibility test-based method with those of the SAT-based method as the second narrow band culling. In an experiment using two models to consist of 2916 and 2731 polygons, respectively, we got potential colliding prism pairs with 99 % of culling rate.

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Metamorphic Malware Detection using Subgraph Matching (행위 그래프 기반의 변종 악성코드 탐지)

  • Kwon, Jong-Hoon;Lee, Je-Hyun;Jeong, Hyun-Cheol;Lee, Hee-Jo
    • Journal of the Korea Institute of Information Security & Cryptology
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    • v.21 no.2
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    • pp.37-47
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    • 2011
  • In the recent years, malicious codes called malware are having shown significant increase due to the code obfuscation to evade detection mechanisms. When the code obfuscation technique is applied to malwares, they can change their instruction sequence and also even their signature. These malwares which have same functionality and different appearance are able to evade signature-based AV products. Thus, AV venders paid large amount of cost to analyze and classify malware for generating the new signature. In this paper, we propose a novel approach for detecting metamorphic malwares. The proposed mechanism first converts malware's API call sequences to call graph through dynamic analysis. After that, the callgraph is converted to semantic signature using 128 abstract nodes. Finally, we extract all subgraphs and analyze how similar two malware's behaviors are through subgraph similarity. To validate proposed mechanism, we use 273 real-world malwares include obfuscated malware and analyze 10,100 comparison results. In the evaluation, all metamorphic malwares are classified correctly, and similar module behaviors among different malwares are also discovered.

Imaging of Facial Nerve With 3D-DESS-WE-MRI Before Parotidectomy: Impact on Surgical Outcomes

  • Han-Sin Jeong;Yikyung Kim;Hyung-Jin Kim;Hak Jung, Kim;Eun-hye Kim;Sook-young Woo;Man Ki Chung;Young-Ik Son
    • Korean Journal of Radiology
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    • v.24 no.9
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    • pp.860-870
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    • 2023
  • Objective: The intra-parotid facial nerve (FN) can be visualized using three-dimensional double-echo steady-state water-excitation sequence magnetic resonance imaging (3D-DESS-WE-MRI). However, the clinical impact of FN imaging using 3D-DESS-WE-MRI before parotidectomy has not yet been explored. We compared the clinical outcomes of parotidectomy in patients with and without preoperative 3D-DESS-WE-MRI. Materials and Methods: This prospective, non-randomized, single-institution study included 296 adult patients who underwent parotidectomy for parotid tumors, excluding superficial and mobile tumors. Preoperative evaluation with 3D-DESS-WE-MRI was performed in 122 patients, and not performed in 174 patients. FN visibility and tumor location relative to FN on 3D-DESS-WE-MRI were evaluated in 120 patients. Rates of FN palsy (FNP) and operation times were compared between patients with and without 3D-DESS-WE-MRI; propensity score matching (PSM) and inverse probability of treatment weighting (IPTW) were used to adjust for surgical and tumor factors. Results: The main trunk, temporofacial branch, and cervicofacial branch of the intra-parotid FN were identified using 3D-DESS-WE-MRI in approximately 97.5% (117/120), 44.2% (53/120), and 25.0% (30/120) of cases, respectively. The tumor location relative to FN, as assessed on magnetic resonance imaging, concurred with surgical findings in 90.8% (109/120) of cases. Rates of temporary and permanent FNP did not vary between patients with and without 3D-DESS-WE-MRI according to PSM (odds ratio, 2.29 [95% confidence interval {CI} 0.64-8.25] and 2.02 [95% CI: 0.32-12.90], respectively) and IPTW (odds ratio, 1.76 [95% CI: 0.19-16.75] and 1.94 [95% CI: 0.20-18.49], respectively). Conversely, operation time for surgical identification of FN was significantly shorter with 3D-DESS-WE-MRI (median, 25 vs. 35 min for PSM and 25 vs. 30 min for IPTW, P < 0.001). Conclusion: Preoperative FN imaging with 3D-DESS-WE-MRI facilitated anatomical identification of FN and its relationship to the tumor during parotidectomy. This modality reduced operation time for FN identification, but did not significantly affect postoperative FNP rates.

New Fast Block-Matching Motion Estimation using Temporal and Spatial Correlation of Motion Vectors (움직임 벡터의 시공간 상관성을 이용한 새로운 고속 블럭 정합 움직임 추정 방식)

  • 남재열;서재수;곽진석;이명호;송근원
    • Journal of Broadcast Engineering
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    • v.5 no.2
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    • pp.247-259
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    • 2000
  • This paper introduces a new technique that reduces the search times and Improves the accuracy of motion estimation using high temporal and spatial correlation of motion vector. Instead of using the fixed first search Point of previously proposed search algorithms, the proposed method finds more accurate first search point as to compensating searching area using high temporal and spatial correlation of motion vector. Therefore, the main idea of proposed method is to find first search point to improve the performance of motion estimation and reduce the search times. The proposed method utilizes the direction of the same coordinate block of the previous frame compared with a block of the current frame to use temporal correlation and the direction of the adjacent blocks of the current frame to use spatial correlation. Based on these directions, we compute the first search point. We search the motion vector in the middle of computed first search point with two fixed search patterns. Using that idea, an efficient adaptive predicted direction search algorithm (APDSA) for block matching motion estimation is proposed. In the experimental results show that the PSNR values are improved up to the 3.6dB as depend on the Image sequences and advanced about 1.7dB on an average. The results of the comparison show that the performance of the proposed APDSA algorithm is better than those of other fast search algorithms whether the image sequence contains fast or slow motion, and is similar to the performance of the FS (Full Search) algorithm. Simulation results also show that the performance of the APDSA scheme gives better subjective picture quality than the other fast search algorithms and is closer to that of the FS algorithm.

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