• 한국구조물진단유지관리공학회 논문집
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• 제11권6호
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• pp.113-120
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• 2007

연약지반에 시공되는 보강토옹벽과 개비온옹벽에 대해서 옹벽의 안정성을 확보하기 위해 필요한 적정 치환영역을 유한요소해석을 통해 검토하였다. 해석은 연약토층의 압밀단계를 포함하여 시공순서를 모사하는 단계별 해석방법으로 수행되었다. 적정 치환영역의 제안을 위한 첫 단계로 치환깊이를 변화시킨 경우들에 대해서 시공단계에 따른 벽체 및 주변 지반의 거동을 조사하는 일련의 해석을 수행하였다. 이들 해석결과를 문헌연구를 통하여 설정한 옹벽의 안정성을 보장할 수 있는 벽체변위, 지반의 침하량 및 전단변형율 등의 한계값과 비교, 평가하였다. 그 결과, 본 연구에서 검토한 지반조건에서 벽체의 높이가 변하는 경우에 대한 적정 치환영역을 벽체의 높이에 대한 치환깊이의 비로 나타내는 시공표준도를 제안하였다

• 한국구조물진단유지관리공학회 논문집
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• 제11권5호
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• pp.197-211
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• 2007

노후화된 PSC Beam 교량은 개선된 보강방안이 필요하다. 본 연구에서는 성능저하가 발생된 노후 PSC Beam 교량을 대상으로 PSC Beam 교량의 보강형을 폐단면 박스형태로 변환하여 이차합성 구조화함으로써, PSC Beam 교량의 강성을 안정적으로 증가시킬 수 있는 박스형 보강방안을 제안하였다. 제안된 보강방안과 기존의 외부프리스트레스 도입공법을 결합하여 보강할 경우, 보강효과를 정량적으로 규명하기 위해 시공단계해석에 따른 시간 의존적 정밀해석을 수행하였다. 성능개선을 위해 제안된 박스형 보강방안 적용 시 보강방법에 따라 전단면 및 휨 보강과 같은 다양한 형태의 보강방안을 제시한 후, 각각의 보강방안에 대해 이차합성 효과에 따른 상향캠버 및 부재응력 특성을 평가하였다. 또한 내하력 평가를 수행하여 내하율을 검토함으로서, 개선된 PSC Beam 교량의 구조 안전성을 정량적으로 평가하였다.

• Genomics & Informatics
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• 제13권2호
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• pp.31-39
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• 2015

Sequencing depth, which is directly related to the cost and time required for the generation, processing, and maintenance of next-generation sequencing data, is an important factor in the practical utilization of such data in clinical fields. Unfortunately, identifying an exome sequencing depth adequate for clinical use is a challenge that has not been addressed extensively. Here, we investigate the effect of exome sequencing depth on the discovery of sequence variants for clinical use. Toward this, we sequenced ten germ-line blood samples from breast cancer patients on the Illumina platform GAII(x) at a high depth of ${\sim}200{\times}$. We observed that most function-related diverse variants in the human exonic regions could be detected at a sequencing depth of $120{\times}$. Furthermore, investigation using a diagnostic gene set showed that the number of clinical variants identified using exome sequencing reached a plateau at an average sequencing depth of about $120{\times}$. Moreover, the phenomena were consistent across the breast cancer samples.

• Journal of Life Science
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• 제10권1호
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• pp.40-44
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• 2000

The SNF2/SW ATPase/helicase family comprises proteins form a variety of species with in vivo functions, such as transcriptional regulation, maintenance of chromosome stability during mitosis, and various types of DNA repair. Here, we reported the characterization of h게2+gene which was iolated by PCR amplification using the conserved domain of SNF2 motifs. Sequence analysis of PCR product showed striking evolutionary conservation among the SNF2 family of proteins. Two transcripts of 6.7 and 3.4 Lb were detected by Northern blot analysis. furthermore, the intensities of these two bands were increased by ultraviolet(UV) irradiation. These results indicate that the hrp2+ is a novel member of the SNF2 family of proteins and is one of the UV-inducible genes in S. pombe. To determine the level of transcripts of hrp2+ gene during cellular growth, Northern blot analysis were performed. This result indicates that the level of hrp2+transcript reached its maximum before cells entered the exponential growth phase. This suggests that hrp2+ gene is experssed mainly at the early stage of cell growth.

• 한국정보통신학회:학술대회논문집
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• 한국해양정보통신학회 2008년도 춘계종합학술대회 A
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• pp.620-623
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• 2008

홈 네트워크 제어 서비스 환경에서는 외부요인에 의한 장애, 네트워크 장애, 장치의 장애 등에 의해 장애가 발생할 수 있다. 더욱이 사용자는 시스템 결함 관리와 같은 복잡한 시스템 관리를 직접하는 것을 원하지 않는다. 따라서 홈 네트워크를 구성하는 홈 기기에 대해 장애가 발생할 경우 자동으로 복구하는 시스템을 필요로 한다. 이를 위해 본 논문에서는 홈 네트워크 제어 시스템 상의 홈 기기들의 제어 로그의 설계와 관리, 장애 발생시점의 홈 기기의 서비스 내용을 분석하여 홈 기기의 서비스 연속성을 보장하기 위한 방법을 제시하고 구현한다.

• Journal of Applied Biological Chemistry
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• 제48권3호
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• pp.120-126
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• 2005

Endothelial nitric oxide synthase (eNOS) plays a critical role in vascular biology and pathophysiology. Its activity is regulated by multiple mechanisms such as calcium/calmodulin, protein-protein interactions, sub-cellular locations and phosphorylation at various sites. Phosphorylation of eNOS-Ser1177 (based on mouse sequence) has been identified as an important mechanism of eNOS activation. However, signaling pathway leading to it phosphorylation remains controversial. The regulation of eNOS-Ser1177 phosphorylation by Src and protein kinase A (PKA) was investigated in the present study using cultured mouse aorta endothelial cells. Expression of a constitutively active Src mutant in the cells enhanced phosphorylation of eNOS and protein kinase B (Akt). The Src-stimulated phosphorylation was not attenuated by the expression of a dominant negative PKA regulatory subunit. Neither activation nor inhibition of PKA activity had any significant effect on tyrosine phosphorylation of activation or inactivation site in Src. Based on the results of this study, it is suggested that Src/Akt pathway and PKA signaling may regulate eNOS phosphorylation independently. The existence of multiple mechanisms for eNOS phosphorylation may guarantee endothelial nitric oxide production in various cellular contexts which is essential for maintenance of vascular health.

• 한국가스학회지
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• 제6권4호
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• pp.1-7
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• 2002

인적 오류(human error)는 공장설비의 설계, 제작, 건설, 운전, 유지$\cdot$보수의 모든 단계에서 발생할 수 있으며 사고의 대부분이 인적 오류의 영향과 관계되어 있는 것으로 조사되고 있다. 본 연구에서는 현장의 작업자 행동 특성 및 오류 메커니즘을 확인하고, 평가분류 쉬트를 활용하여 공정에서 발생하는 인적 오류를 분석하였다. 또한 ASEP HRA 절차를 이용하여 인적 오류 확률(HEP) 산정 알고리즘을 구축하여 현장에서 쉽게 인적 오류를 분석할 수 있는 ASEP HEP 프로그램을 개발하였다. 이를 이용하여 화학공장에서의 가능한 인적 오류사고를 예방하고 보다 체계적인 인적 오류 방지대책을 수립할 수 있다고 판단된다.

• Food Science and Biotechnology
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• 제15권6호
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• pp.896-901
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• 2006

Patulin is a food mycotoxin which induces genotoxicity and acute intestinal disease in infants. Patulin mainly originates from fruit putrefactive moulds, especially in apples, which necessitates the maintenance of strong safety standards against patulin for fresh and processed apples. To investigate the patulin producing moulds in Korean apples, 16 morphological types of fungi were isolated from Korean apples and a patulin producing fungus was identified based on a sequence analysis of the region of internal transcribed spacers (ITS5-5.8S-ITS4 region, 505 base pair) and the 26 rRNA D1/D2 region (527 base pair). Morphological analyses were also performed. The isolated patulin producing fungus was found to a representative species of Penicillium crustosum. The maximal patulin production ability of the isolated fungus (P. crustosum) and the patulin producing standard strain (P. griseofulvum, ATCC 46037) in an SY broth medium were 0.32 and 2.46 mg/L, respectively.

• 대한전기학회논문지:전력기술부문A
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• 제49권7호
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• pp.359-364
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• 2000

This paper is introducing a newly developed spark-gap switch with dual trigger system, into which the current from the voltage source is injected along with the test sequence during the synthetic testing of high voltage circuit-breaker. The currently-used spark gap switch is narrow in operating range due to the use of the method of triggering energy being injected by single way. As a result, the frequent happening of misoperation has greatly reduced the test quality and test efficiency and has required the cost of maintenance excessively. In this study, accordingly, in order to basically remove these problems, another triggering system is installed to the opposite direction on the existing triggering system; attaching the same time and the same rising time of pulse wave as on the existing system, so that at a comparatively trigger gap distance from the main electrode(the gap can be operated at 60% of self-break voltage, while at 80% in the current system), the main electrode has been enabled to be closed by the development of spark gap switch with dual trigger system.

• Molecules and Cells
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• 제19권3호
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• pp.303-309
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• 2005

The end of eukaryotic genomic DNA is capped by a specialized structure called as "telomere" which consists of the repetitive array of nucleotide sequence, TTAGGG, in humans and mice, and a variety of binding proteins. Telomerase is a ribonucleoprotein (RNP) complex responsible for the elongation of telomeres to maintain the genomic integrity, and is composed of telomerase reverse transcriptase (TERT), telomerase RNA component (TERC), and their associated factors regulating the catalytic activity of telomerase. Although it is now apparent that telomerase protects cells from apoptosis via the maintenance of genomic integrity by stabilizing telomeres, our understanding for the physiological role of telomerase is yet far from completion, and emerging evidence suggests that telomerase has additional extratelomeric roles in mediating cell survival and anti-apoptotic functions against various cytotoxic stresses. Here we summarize and discuss how telomerase and telomeres are involved in mediating cellular protection against apoptosis.