• Fisheries and Aquatic Sciences
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• v.19 no.2
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• pp.8.1-8.11
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• 2016

To quantitatively estimate the influence of cuttlebone on the target strength (TS) of golden cuttlefish, the cuttlebone was carefully extracted from 19 live cuttlefish caught using traps in the inshore waters around Geojedo, Korea, in early May 2010 and the TS was measured using split-beam echosounders (Simrad ES60 and EY500). The TS-length relationships for the cuttlefish (before the extraction of cuttlebone, Fish Aquat Sci. 17:361-7, 2014) and the corresponding cuttlebone were compared. The cuttlebone length ($L_b$) ranged from 151 to 195 mm (mean $L_b$ = 168.3 mm) and the mass ($W_b$) ranged from 29.3 to 53.2 g (mean $W_b$ = 38.8 g). The mean TS values at 70 and 120 kHz were -33.60 dB (std = 1.12 dB) and -32.24 dB (std = 1.87 dB), respectively. The mean TS values of cuttlebone were 0.19 dB and 0.04 dB lower than those of cuttlefish at 70 and 120 kHz, respectively. For 70 and 120 kHz combined, the mean TS value of cuttlebone was -32.87 dB, 0.11 dB lower than that of cuttlefish (-32.76 dB). On the other hand, the mean TS value of cuttlebone predicted by the regression ($TS_b$ = 24.86 $log_{10}$ $L_b$ - 4.86 $log_{10}$ ${\lambda}$ - 22.58, $r^2$ = 0.85, N = 38, P < 0.01) was -33.10 dB, 0.04 dB lower than that of cuttlefish predicted by the regression ($TS_c$ = 24.62 $log_{10}$ $L_c$ - 4.62 $log_{10}$ ${\lambda}$ - 22.64, $r^2$ = 0.85, N = 38, P < 0.01). That is, the contribution of cuttlebone to the cuttlefish TS determined by the measured results was slightly greater than that by the predicted results. These results suggest that cuttlebone is responsible for the TS of cuttlefish, and the contribution is estimated to be at least 99 % of the total echo strength.

• Korean Journal of Fisheries and Aquatic Sciences
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• v.36 no.3
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• pp.198-203
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• 2003

Heat-induced surimi gels were prepared using various concentration of ATC as a additives and calcium agent. Regardless of various concentration of ATC, there were no difference the moisture $(80.4-81.2\%)\;and\;crude\;ash\;(1.4-1.\5%)$ contents. The pH of heat-induced surimi gels were decreased 7.16 to 7.04 depend on increasing ATC concentration. The whiteness, breaking force and gel strength of $0.09\%$surimi gel were improved significantly difference (p<0.05). Sensory evaluation on texture and whiteness were also similar to determination by color and texture meters. In mineral content of heat-induced surimi gel calcium content was increased 26 to 54 mg/100g depend on increasing ATC concentration, while phosphorus content was not change. The optimal concentration of ATC for preparation of high quality heat-induced surimi gel was $0.09\%$. The shelf-life of heat-induced surimi gel did not extend by addition of $0.09\%$ ATC.

• Korean Journal of Fisheries and Aquatic Sciences
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• v.36 no.3
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• pp.204-209
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• 2003

Utilization of soluble protein recovered from surimi wastewater using calcium powder of cuttle bone were examined. The crude ash content of the heat-induced surimi gel was increased linearly by increasing substitution ratio of recovered protein-ATC toward commercial surimi. Moisture (approximately $76\%$) and lipid $(0.2\%)$ contents were not change, but their protein contents were decreased 15.7 to $14.3\%$ depend on increasing of substitution ratio. The white index of the heat-induced surimi gel by color meter was increased up to $10\%$ of substitution ratio. There were no difference between $0\%\;and\;5\%$ substituted surimi gel in the gel strength. The sensory score on white index and texture of the heat-induced surimi gel did not change in 0 to $10\%$ as a substitution ratio of recovered protein-ATC toward commercial surimi, while decreased in more $15\%.$ The optimal substitution ratio of recovered protein-ATC as a bulking agent was $10\%.$ The heat-induced surimi gel prepared with $10\%$ substitution of recovered protein-ATC was similar to the content and composition of total amino. acids, and superior to calcium content and the ratio of calcium and phosphorus toward those of commercial surimi.

• Journal of the Korean Society of Fisheries and Ocean Technology
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• v.47 no.2
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• pp.88-98
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• 2011

Experimental fishing was carried out to compare the catches of main species and bycatch according to the codend mesh size (raschel, 15mm, 25mm, 35mm, 45mm) of stow net on anchor in the coastal waters of Seocheon after manufacturing five kinds of fishing gears and commercial fishing boat (7.93 tons) was used for it. Anchovy (Engraulis japonicus), southern rough shrimp (Trachysalambria curvirostris), beka squid (Loligo beka), cuttle fish (Sepia esculenta), swimming crab (Portunus trituberculatus) were dominant on the five kinds of experimental fishing gears. Southern rough shrimp was caught from all kinds of fishing gears and catches of anchovy was highest in the raschel codend, southern rough shrimp for 15mm, 25mm, 35mm codend and beka squid for 45mm codend. Bycatch proportion against total catches was 623,376g (28.7%) for 2,171,844g in raschel, 11,932g (1.7%) for 704,451g in 15mm, 3,616g (1.6%) for 221,432g in 25mm, 9,276g (4.9%) for 191,052g in 35mm and 2,724g (5.3%) for 51,431g in 45mm.

• Journal of the Korean Society of Fisheries and Ocean Technology
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• v.46 no.1
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• pp.1-9
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• 2010

Experimental fishing was carried out to compare fishing status according to the codend mesh size (raschel, 15mm, 25mm, 35mm, 45mm) of stow net on anchor in the coastal waters of Seocheon after manufacturing five kinds of fishing gears and commercial fishing boat (7.93 tons) was used for it. Total catches of raschel, 15mm, 25mm, 35mm, 45mm codend were 816,949g, 203,994g, 1,405g, 51,576g, 194g in September and 40,545g, 66,974g, 14,692g, 12,647g, 12,655g in October. Dominance species were anchovy (Engraulis japonicus) in raschel codend, largehead hairtail (Trichiurus lepturus) in 15mm and 35mm in September, and the amount of catches was very small in 25mm and 45mm codend due to the fishing gear damage. In October, dominance species were anchovy and beka squid (Loligo beka) in raschel, 15mm, 25mm codend, and cuttlefish (Sepia esculenta) and beka squid were mainly caught in 35mm and 45mm codend. In addition, total length of anchovy and cardinal fish (Apogon lineatus) were increased according to the increase of codend mesh size but there was no difference in the mantle length of cuttlefish and loligo beka.

• Journal of Food Hygiene and Safety
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• v.33 no.4
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• pp.280-288
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• 2018

In this study, an approach for the analysis of the five cephalopod species (octopus, long-arm octopus, squid, wet-foot octopus, beka squid) consumed in the Republic of Korea is developed. The samples were collected from the Southeast Asian countries Thailand, Indonesia, Vietnam, and China. The SYBR-green-based real-time qPCR method, based on the mitochondrial DNA genome of the five cephalopods was developed and validated. The intergroup variations in the mitochondrial DNA are evident in the bioinformatic analysis of the mitochondrial genomic DNA sequences of the five groups. Some of the highly-conserved and slightly-variated regions are identified in the mitochondrial cytochrome-c-oxidase subunit I (COI) gene, 16s ribosomal RNA (16s rRNA) gene, and 12s ribosomal RNA (12s rRNA) gene of these groups. To specify each five cephalopod groups, specific primer sets were designed from the COI, 16s rRNA and 12s rRNA regions. The specific primer sets amplified the DNA using the SYBR-green-based real-time PCR system and 11 commercially secured animal tissues: Octopus vulgaris, Octopus minor, Todarodes pacificus, Dosidicus gigas, Sepia esculenta, Amphioctopus fangsiao, Amphioctopus aegina, Amphioctopus marginatus, Loliolus beka, Loligo edulis, and Loligo chinensis. The results confirmed by a conveient way to calculate relative amplification levels between different samples in that it directly uses the threshold cycles (Ct)-value range generated by the qPCR system from these samples. This genomic DNA-based molecular technique provides a quick, accurate, and reliable method for the taxonomic classification of the animal tissues using the real-time qPCR.