• BMB Reports
• /
• v.31 no.4
• /
• pp.405-408
• /
• 1998

Endonuclease-sensitive sites detected by T4 endonuclease V or UvrABC nuclease treatments were compared in the dihydrofolate reductase gene of UV-irradiated Chinese hamster ovary B-11 cells. The number of endonuclease-sensitive sites detected by T4 endonuclease V treatment followed by NaOH denaturation was twice that of formamide denaturation. Repeated treatment of damaged genomic DNA with T4 endonuclease V resulted in no further increase in the number of endonuclease-sensitive sites detected. The numbers of endonuclease-sensitive sites detected by UvrABC nuclease using each denaturation condition were similar. Sequential treatment with the two endonucleases using formamide denaturation resulted in twice the number of endonuclease-sensitive sites detected by treatment of each nuclease alone. Due to a lack of AP endonuclease activity these results suggest the presence of T4 endonuclease V-sensitive sites which could be complemented by alkaline gel separation or by UvrABC nuclease treatment.

• Proceedings of the Korean Society for Noise and Vibration Engineering Conference
• /
• 1998.04a
• /
• pp.582-589
• /
• 1998

This paper presents the analysis results of ex-core and in-core neutron noise, acceleration signals and pressure fluctuation measured at Ulchin Nuclear Unit 1 & 2 to identify and monitor the reactor internals vibration including fuel motion. A phase separation algorithm developed by authors was applied to the neutron noises to clearly identify the reactor internals vibratory motion. The beam mode frequency of the core support barrel was identified to be 8Hz and the shell mode to be 20Hz. The first frequency of the fuel assembly was also found to be 3Hz, while first two acoustic frequencies of the primary coolant system were 6 and 17.5Hz. By monitoring and analyzing these frequencies periodically, it is possible to diagnose the operating condition of reactor internals and to provide an early detection of faults for the predictive maintenance.

• Journal of the Korean Graphic Arts Communication Society
• /
• v.22 no.2
• /
• pp.123-138
• /
• 2004

The video phone tube (VPT) of monochrome CRT have utilized home door phone, fish-finder and the rear watch monitors. Phosphor screen formation is made by electrodeposition spin coating and thermal transfer methods etc. Recently, thermal transfer method was developed, as a novel method, to form the phosphor surface for mnonchrom VPT. This method have advantages of simple process, automatization, clean environment, saving raw material and saving running-cost. In this study, it was developed new phosphor of VPT, and tested about phosphor paste properties. An experimental studies of VPT as a new phosphor property and improved VPT's manufacturing process shortening and brightness. As thermal transper method is a paste processing, it is important that rheology of phosphor effects on the formation of phosphor screen. Hence this paper was studied rheology properties of phosphor paste and the formation of phosphor screen had looked most suitable condition. Experimented thermal separation properties of low calcination temperature resin and the result analyzed comparison by TGA. Also, examined calcination properties to reduce remaining binder phosphor.

• Transactions of the Korean Society of Pressure Vessels and Piping
• /
• v.12 no.1
• /
• pp.85-92
• /
• 2016

The operating temperature of VHTR components is much higher than that of conventional PWR due to high core outlet temperature of VHTR. Material requirements and technical issues of VHTR reactor components which are mainly dominated by high temperature service condition were discussed. The codification effort for high temperature material and design methodology are explained. The design class for VHTR components are classified as class A or B according to the recent ASME high temperature reactor design code. A separation of thermal boundary and pressure boundary is used for VHTR components as an elevated design solution. Key design characteristics for reactor pressure vessel, control rod, reactor internals, graphite reflector, circulator and intermediate heat exchanger were analysed. Thermo-mechanical analysis of the process heat exchanger, which was manufactured for test, is presented as an analysis example.

• Fisheries and Aquatic Sciences
• /
• v.15 no.2
• /
• pp.107-115
• /
• 2012

In developing a useful tool to detect parasitic dynamics in an estuarine ecosystem, a denaturing high-performance liquid chromatography (DHPLC) assay was optimized by cloning plasmid DNA from the grass shrimp Palaemonetes pugio, and its two parasites, the trematode Microphallus turgidus and bopyrid isopod Probopyrus pandalicola. The optimal separation condition was an oven temperature of $57.9^{\circ}C$ and 62-68% of buffer B gradient at a flow rate of 0.45 mL/min. A peptide nucleic acid blocking probe was designed to clamp the amplification of the host gene, which increased the amplification efficiency of genes with low copy numbers. Using the DHPLC assay with wild-type genomic, the assay could detect GC Gram positive bacteria and the bopyrid isopod (P. pandalicola). Therefore, the DHPLC assay is an effective tool for surveying parasitic dynamics in an estuarine ecosystem.

• Journal of the Korea Academia-Industrial cooperation Society
• /
• v.10 no.4
• /
• pp.865-871
• /
• 2009

Chondroitin sulfate was extracted by alkali method and enzyme method from shark cartilage. In extract system, various processing parameters such as concentration of alkali and alcarase, temperature etc, have been investigated for optimization condition. The pure chondroitin sulfate was obtained by UF membrane separation. The characteristics was also investigated with gel permeation chromatograpy(GPC). The molecular weight of chondroitin sulfate was $2.7\times10^4$ Da.

• Journal of the Korean Magnetic Resonance Society
• /
• v.13 no.1
• /
• pp.7-14
• /
• 2009

The spectral data were collected from the two 600 MHz spectrometers from the two major manufacturers, Broker and Varian. The samples were prepared to create standard curves for quantitative measurements of metabolite concentrations. Instead of employing one-dimensional $^1H$ experiments, the two-dimensional $^1H-^{13}C$ HSQC experiments were performed for better separation of resonances. For some resonances, the high salt condition hindered the linear correlation between the intensity and actual metabolite concentration. Excluding overlapped ones, most resonances showed good linearity. Although the Varian spectrometer showed better linearity, both spectrometers were able to generate acceptable standard curves. From this data, we could identify resonances that could be used to better quantify the concentrations of the particular metabolites. With these standard curves, the quantitative measurements of the metabolites from the real samples will be facilitated.

• Proceedings of the Korean Society of Soil and Groundwater Environment Conference
• /
• 2001.09a
• /
• pp.216-219
• /
• 2001

In this study, a hydrocyclone operated by centrifugal force was examined as a part of dredge system of lake sediments. It does not help dehydration of the sediments, but also enables the classification of fine particles in the stable and sanitary treatment. Several experiments were performed focusing on the separation efficiency under the condition of room temperature with virtual sediment samples and simulated particles. As a result of the experiment, it was found that the apex size of the cyclone greatly affected the cut diameter(d$_{50}$), and high density particles were separated more effectively.y.

• Journal of Institute of Control, Robotics and Systems
• /
• v.11 no.11
• /
• pp.901-906
• /
• 2005

The stability robustness problem of continuous linear systems with nominal and delayed time-varying perturbations is considered. In the previous results, the entire bound was derived only for the overall perturbations without separation of the perturbations. In this paper, the sufficient condition for stability of the system with two perturbations, which are nominal and delayed, is expressed as linear matrix inequalities(LMIs). The corresponding stability bounds fer those two perturbations are determined by LMI(Linear Matrix Inequality)-based generalized eigenvalue problem. Numerical examples are given to compare with the previous results and show the effectiveness of the proposed.

• Journal of Industrial Technology
• /
• v.22 no.B
• /
• pp.21-26
• /
• 2002

The non-dimensional heat loss from a thermally asymmetric triangular fin is investigated as a function of a ratio of upper and lower surface Biot numbers (Bi2/Bi1), the non-dimensional fin length and tip surface Biot number using the two-dimensional separation of variables method. The effect of fin tip surface Biot number on the variation of the non-dimensional temperature along the sloped upper and lower surfaces for the thermally asymmetric condition is presented. The relationship between the non-dimensional fin length and the fin tip surface Biot number for equal amount of heat loss is also discussed as well as the relationship between upper surface Biot number and tip surface Biot number for equal amount of heat loss.