• 제목/요약/키워드: Separation Ability

검색결과 211건 처리시간 0.026초

Purification and Structural Analysis of Surfactin Produced by Endophytic Bacillus subtilis EBS05 and its Antagonistic Activity Against Rhizoctonia cerealis

  • Wen, Cai-Yi;Yin, Zhi-Gang;Wang, Kai-Xuan;Chen, Jian-Guang;Shen, Shun-Shan
    • The Plant Pathology Journal
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    • 제27권4호
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    • pp.342-348
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    • 2011
  • Bacillus subtilis EBS05, an endophytic bacteria strain isolated from a medicinal plant Cinnamomum camphor, can produce antagonistic compounds that effectively inhibit plant pathogenic fungi. The greenhouse experiments showed that wheat sharp eyespot disease (WSED) was reduced by 91.2%, 88.2% and 43.0% after the treatment with fermentation broth, bacteria-free filter and a fungicide fludioxonil, respectively. The culture broth of strain EBS05 can more effectively control WSED than can fludioxonil. The fermentation broth and bacteria-free filter ability to suppress WSED was not significantly different, suggesting that an active secreted substance played a major role in controlling WSED. Separation and purification of the active compounds was carried out by serial processes, including hydrochloric acid (pH 2.0) treatment, methanol extraction and Sephadex LH-20 column chromatography, silica gel column chromatography and reverse-phase high-pressure liquid chromatography (HPLC), respectively. The purified compounds, one of active peaks in the HPLC spectrum, were obtained from the collection. Analysis of the chemical structures by time-of-flight mass spectrometry (TOF-MS) and electrospray ionization mass spectrometry/mass spectrometry (ESI-MS/MS) showed that the active substances produced by the endophytic bacteria EBS05 are mixture of the ${\beta}$-hydroxy-C12~C15-$Leu^7$ surfactin A isomers with 1035.65 Da, 1021.64 Da, 1007.63 Da and 993.65 Da molecular weights, respectively.

정수지 내부 단락류 발생 평가 : Part A. 정수장 내부 단락류 분석을 통한 장폭비와 형태가 T10/T 값에 미치는 영향 연구 (Internal Short-circuiting Estimation in Clearwell : Part A. Improving T10/T Using Intra Basin and Diffuser Wall by Applying ISEM to Field)

  • 신은허;이승재;김성훈;박희경
    • 상하수도학회지
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    • 제22권1호
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    • pp.105-112
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    • 2008
  • Disinfection is a basic and effective microorganism inactivation method and historically contributed a decrease in waterborne diseases. To guarantee the disinfection ability, improving T in CT value is important. Many indexes are used to estimate the hydraulic efficiency, however, these are black-box analysis. Therefore it is need to develope new estimation method. In this study, internal short-circuiting estimation method (ISEM) is developed using CFD and we inquire into the factor which causes increase of $T_{10}/T$ value as LW ratio increases. And the effect of shape on the relation of LW ratio and $T_{10}/T$ is analyzed. As LW ratio increases, internal short-circuiting index (ISI) of influent and effluent zone is rapidly reduced and recirculation and dead zone are reduced in channel zone. Therefore, as the $T_{10}/T$ value converges maximum value, ISI curve is changed from "V" shape to "U" shape and hydraulic efficiency is improved especially in downstream portion of clearwell. The less the shape ratio(width/length of clearwell) is the less the $T_{10}/T$ value is at a same LW ratio because the portion of turning zone increases as shape ration decreases, therefore more boundary separation is generated.

Breakdown Characteristics of SF6 and Liquefied SF6 at Decreased Temperature

  • Choi, Eun-Hyeok;Kim, Ki-Chai;Lee, Kwang-Sik
    • Journal of Electrical Engineering and Technology
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    • 제7권5호
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    • pp.765-771
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    • 2012
  • $SF_6$ gas has been used as arc quenching and insulating medium for high and extra high voltage switching devices due to its high dielectric strength, its excellent arc-quenching capabilities, its high chemical stability and non toxicity. Despite of its significant contributions, the gas was classified as one of the greenhouse gas in the Kyoto Protocol. Thus, many researches are conducted to find out the replacement materials and to develop the $SF_6$ gas useless electrical equipment. This paper describes experiments on the temperature change-related breakdown characteristics of $SF_6$ gas ($SF_6$) and $SF_6$ liquid ($LSF_6$) in a model GIS(Gas-Insulated Switchgear) chamber in order to show the possibility of more stable and safe usages of $SF_6$ gas. The breakdown characteristics are classified into three stages, namely the gas stage of $SF_6$ according to Paschen's law, the coexisting stage of $SF_6$ gas with liquid in considerable deviation at lower temperature, and the stage of $LSF_6$ and remaining air. The result shows that the ability of the $LSF_6$ insulation is higher than the high-pressurized $SF_6$. Moreover, it reveals that the breakdown characteristics of $LSF_6$ are produced by bubble-formed $LSF_6$ evaporation and bubbles caused by high electric emission and the corona. In addition, the property of dielectric breakdown of $LSF_6$ is determined by electrode form, electrode arrangement, bubble formation and movement, arc extinguishing capacity of the media, difficulty in corona formation, and the distance between electrodes. The bubble formation and flow separation phenomena were identified for $LSF_6$. It provides fundamental data not only for $SF_6$ gas useless equipment but also for electric insulation design of high-temperature superconductor and cryogenic equipment machinery, which will be developed in future studies.

부탄올의 분사 및 분무특성에 관한 실험적 연구 (An experimental study on the injection and spray characteristics of butanol)

  • 정탁수;왕우경;김상암
    • 수산해양기술연구
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    • 제53권1호
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    • pp.89-97
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    • 2017
  • Butanol has an ability to improve the ignition quality due to its lower latent heat of vaporization; it has an advantage to reduce a volume of a fuel tank because its energy density is higher than that of ethanol. Also, butanol-diesel oil blending quality is good because butanol has an effect to prevent the phase-separation between two fuels. Even if the blended oil contains water, it can reduce the corrosion of the fuel line. Thus, it is possible to use butanol-diesel oil blended fuel in diesel engine without modification, and it may reduce the environment pollution due to NOx and particulate and the consumption of diesel oil. Therefore, some studies are being advanced whether butanol is adequate as an alternative fuel for diesel engines, and the results of the combustion and exhaust gas emission characteristics are being presented. Though the injection and spray characteristics of butanol are more important in diesel combustion, the has not yet dealt with the matter. In this study, the influence in which differences of physical properties between butanol and diesel oil may affect the injection and spray characteristics such as injection rate, penetration, spray cone angle, spray velocity and process of spray development were examined by using CRDI system, injection rate measuring device and spray visualization system. The results exhibited that the injection and macroscopic spray characteristics of two fuels were nearly the same.

위성영상 및 지구물리 영상자료의 호모몰픽 필터링 적용 (Application of Homomorphic Filtering to Satellite Imagery and Geophysical Image Data)

  • 류희영;이기원;권병두
    • 한국지구과학회지
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    • 제26권1호
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    • pp.58-65
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    • 2005
  • 호모몰픽 필터링은 푸리에 변환에 기초를 둔 기법으로 주파수 영역에서 저주파 신호는 약화시키고 고주파 신호는 강화하여 영상의 대비 차를 강화시키는 처리기법이다. 호모몰픽 필터링을 위한 응용 프로그램을 개발하여 인공위성영상과 지구물리 자력탐사 자료를 이미지화한 영상에 시험적으로 적용하여 그 결과를 분석하였다. 영상평활화 기법이나 커널 마스크 처리 등과 같은 영상강화 기법에서는 추출 가능한 경계부의 위치를 변화시키거나 영상의 화소값이 전체 영상을 대상으로 변화시키는 반면에 호모몰픽 필터링은 세부적인 영상 정보의 내용을 선택적으로 강조할 수 있다. 호모몰픽 필터링은 인공위성 영상에서 복잡한 지형지물의 특성을 추출하거나 분리하는 데 효과적인 방법으로 나타났으며 지구물리 영상자료에서 이상대를 조사하는 경우에도 유용하게 적용될 수 있을 것으로 생각된다.

Recovery of Palladium from a Mixture of Pt, Pd and Rh by Solvent Extraction

  • Kim, berly S. Svalstad;Kim, Nam-Soo;Kenneth N. Han
    • 대한전자공학회:학술대회논문집
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    • 대한전자공학회 2001년도 The 6th International Symposium of East Asian Resources Recycling Technology
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    • pp.482-488
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    • 2001
  • Platinum group metals (pgm) are useful to many industries such as chemical, dental and medical, petroleum, refining, electrical and electronic, and automotive. Researchers at the South Dakota School of Mines and Technology and PGM Recovery Ltd. have developed jointly an environmentally sound and metallurgically efficient process for extracting these metals from secondary sources. Once these metals have been dissolved in the leach liquor, the individual metals mainly platinum, palladium, and rhodium, should be separated in order to recover the individual metals with high purity. During this investigation, solvent extraction has been chosen as the method used to achieve the separation and extraction of platinum, palladium, and rhodium from the leach liquor. There were three solutions used throughout this procedure: 1) Synthetic solution (200 ppm Pt 80 ppm Pd 20 ppm Rh; 300 ppm Pt, 180 ppm Pd 50 ppm Rh), and 2) Auto catalyst leach liquors (100 ppm Pt, 30 ppm Pd, 20 ppm Rh). The solvents investigated included Lix 84(2-hydroxy-5-nonylacetonphenone oxime in a mixture with 5-dodecylsalicyloxime), Lix 84-I, ACORGA CLX-50 (diester of pyridine 3,5 dicarboxylic acid), and di-hexyl sulfide. The extraction values achieved using ACORGA CLX-50, Lix 84, and Lix 84-I were respectively Pt (25%, 0% 0%), Pd (100%, 99.8%, 95.3%), and Rh (99.1%, 35.5%, 4.25%). The stripping processes for the Lix 84, and Lix 84-I were proven to be more involved than others. The solutions were required to be simultaneously heated and stirred. The percentages acquired through these processes yielded unsatisfactory results. The stripping procedure for the ACORGA CLX-50 was easier to execute, yet the percentage recovered from this process was also unsatisfactory. Overall the di-hexyl sulfide has proved to be the most successful organic for this procedure. The average percent extracted for palladium was excellent with 99.9% - 100% with very little Platinum and rhodium extracted. The ability of stripping palladium in ammonia solution was also found to be excellent.

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철도시설에 있어서의 구조물 거동대응형 방수기술의 적용 (Structure movement-coping Waterproofing technology application for Railroad facilities)

  • 조일규;이종용;오상근
    • 한국철도학회:학술대회논문집
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    • 한국철도학회 2010년도 춘계학술대회 논문집
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    • pp.1964-1969
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    • 2010
  • 최근 건설시장 규모가 확대되고 교통산업이 발전하면서 지하철 및 터널 등과 같은 개착형 Box 혹은 Shield형 터널 구조물 등 지하구조물의 건설이 다양화되고, 그 수요도 점차 급증하고 있는 실정이다. 그러나 주로 지하에 건설되는 콘크리트 구조물은 시공시 신축이음 및 시공 조인트 등과 같은 다양한 형태의 조인트 발생과 구조물의 수축팽창에 따른 거동, 부동침하, 과다하중, 진동발생에 의한 균열로 인하여 누수가 발생되고 있다. 이에 다양한 방수공법 및 재료들이 현장에 적용되고는 있지만 지하철도, 지하철 등과 같은 진동 및 구조물의 거동이 상시적으로 발생되는 환경조건에서는 방수층의 성능이 저하되어 균열, 침식, 들뜸 등으로 물이 확산되어 재누수가 되는 악순환을 겪고 있는 실정이다. 따라서 본 연구에서는 진동 및 구조물의 거동에 대응 가능한 점착 유연형 복합방수공법을 대상으로 구조물의 거동 대응성 시험을 평가하고, 거동 및 진동이 상시적으로 발생되는 지하철, 터널 등을 대상으로 실제 현장에 적용 후 사례를 통해 구조물 거동에 대응 가능한 방수기술을 검토하였다. 이러한 거동 대응 특성을 검토한 결과 방수재가 기건 및 수중환경에서도 재료의 유연성과 부착성을 유지하여 물의 침투를 방지하고, 거동폭 $5.0mm({\pm}0.5)$ 범위에서도 거동에 대응 가능함을 알 수 있었다.

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Pulse 전위를 적용한 전기화학적 증착 공정으로 제조된 가시광 활성 이종접합 $CuInS_2-TiO_2$ Nanotube 화합물 광전극 (Visible Light-Driven $CuInS_2-TiO_2$ Nanotube Composite Photoelectrodes with Heterojunction Structureusing Pulsed-Electrochemical Deposition Process)

  • 윤정호;;박영구
    • 한국응용과학기술학회지
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    • 제30권1호
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    • pp.49-56
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    • 2013
  • Excellent electron transport properties with enhanced light scattering ability for light harvesting have made well-ordered one dimensional $TiO_2$ nanotube(TNT) arrays an alternative candidate over $TiO_2$ nanoparticles in the area of solar energy conversion applications. The principal drawback of TNT arrays being activated only by UV light has been addressed by coupling the TNT with secondary materials which are visible light-triggered. As well as extending the absorption region of sunlight, the introduction of these foreign components is also found to influence the charge separation and electron lifetime of TNT. In this study, a novel method to fabricate the TNT-based composite photoelectrodes employing visible responsive $CuInS_2$ (CIS) nanoparticles is presented. The developed method is a square wave pulse-assisted electrochemical deposition approach to wrap the inner and outer walls of a TNT array with CIS nanoparticles. Instead of coating as a dense compact layer of CIS by a conventional non-pulsed-electrochemical deposition method, the nanoparticles pack relatively loosely to form a rough surface which increases the surface area of the composite and results in a higher degree of light scattering within the tubular channels and hence a greater chance of absorption. The excellence coverage of CIS on the tubular $TiO_2$ allows the construction of an effective heterojunction that exhibits enhanced photoelectrochemical performance.

열에너지 저장소 내 열성층화를 평가하기 위한 기법 (Methods to Characterize the Thermal Stratification in Thermal Energy Storages)

  • 박도현;류동우;최병희;선우춘;한공창
    • 터널과지하공간
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    • 제23권1호
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    • pp.78-85
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    • 2013
  • 열에너지를 성층화하여 저장하는 주된 목적은 에너지의 열역학적 질을 유지하기 위한 것으로서 열에너지의 성층화를 통해 필요시 원하는 온도에서 열에너지 활용이 가능하다. 저장소 내 열에너지의 온도에 따른 분리, 즉 열성층화는 이와 같은 열에너지의 활용에 영향을 미치는 핵심 인자이다. 본 논문에서는 열성층화의 정도를 평가할 수 있는 기존에 제안된 기법들을 소개하였으며, 특히 열에너지의 주입, 저장, 배출 과정 동안 열저장소의 성층화와 관련된 성능을 결정하는 데 사용될 수 있는 기법들을 중심으로 개념 및 특징을 살펴보았다. 또한 열성층화 지수를 이용하는 방법을 토대로 스웨덴 Lyckebo 암반공동 내 열에너지의 성층도를 비교 분석하여 기법의 적용성을 조사하였다.

한국내 물오리나무(Ainus hirsuta) 집단의 공간적 상관관계 (Spatial Autocorrelation within a Korean Population of Alnus hirsuta)

  • Park, Joo-Soo
    • 생명과학회지
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    • 제14권2호
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    • pp.345-350
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    • 2004
  • 한국내 물오리나무(Ainus hirsuta) 집단의 미소지리적 변이를 공간적 상관관계에 적용하였다. 분리된 등급간 대립유전자의 각 형태의 산출을 정규분포 편차 통계에서 임의분포를 하고 있는지를 검정하였다. 모든 경우에서 예상 값이 유의성을 가지는지 Moran의 I값으로 추정한 결과 120 경우 준 24 경우(20.0%)에서 유의성을 나타내었다. 이들 값 중에서 17 경우는 음의 값을 나타내었는데 이는 한국의 물오리집단이 10등급 간격에서 유사하지 않다는 점을 시사하였다. 그 한 원인으로는 화목(火木)을 위한 인간의 간섭을 추정할 수 있다. 열매를 맺는 가지나 줄기의 간헐적인 벌목에 의해 유전자 유동이 조장되었기 때문일 것이다. 한편, 그루터기나 밑둥으로부터 영양번식을 할 수 있는 능력 또한 유전적 구조를 어지럽히는 한 요인으로 작용한 것으로 사료된다.