• Clinical and Experimental Reproductive Medicine
• /
• 제20권2호
• /
• pp.107-115
• /
• 1993

Procedures to separate motile. normal & motile and acrosome-reacted sperm with high efficiency have clinical application in Assisted Reproductive Technology in terms of increasing the probability of fertilization by a normal sperm and subsequent normal embryonic development. This study evaluated the effects of 10 sperm preparation techniques [Swim-up from a washed pellet (SU). Swim-up from semen (SO). Continuous Percoll Gradients I (PIC). Discontinuous Percoll Gradients I (PID). Continuous Percoll Gradients II(P II C). Discontinuous Percoll Gradients II(P II D), SpermPrep (SFC). Wang's tube (WT). Albumin Gradients (AG), Low temperature capacitation (LTC)] on motility (%), normal morphology (%), motile sperm recovery rate(%). morphologically normal & motile sperm recovery rate (%), true acrosome reaction (%) and fertilizing ability. A P II D proved to be an effective means of separating morphologically normal & motile sperm. Our results indicated the P II D has advantages as compared with other methods in terms of recovery rate. enhancement of motility and normal morphology. And a LTC seems to be an effective means of enhancing the true acrosome reaction and fertilizing ability. These results suggest that the combined method of LTC and P II D for separation of morphologically normal & motile sperm and acrosome reacted sperm may be a useful procedure for intrauterine insemination and in vitro fertilization in the management of male factor infertility as well as for isolation of subpopulation of sperm for basic research.

• 한국치위생학회지
• /
• 제10권5호
• /
• pp.877-885
• /
• 2010

Objectives : This study was carried out with the aim of utilizing it as basic data in a plan for oral health promotion by analyzing oral-health awareness in high school students of some districts in our country, and by analyzing physical, mental and social influential factors in oral health, through using OHIP(Oral Health Impact Profile). Methods : It surveyed by carrying out self-administered questionnaire research targeting 536 boy & girl high school students in the 1st and the 2nd grade at 3 high schools in some districts of Gyeonggi Province. Results : 1. As for experience of having not visited dental clinic, a case of having not visited accounted for 32.5%. On the other hand, a case of having visited accounted for 67.5%. As for the appearance of oral health education, only 2.8% responded as saying of having experience. 97.2% responded as saying of having not taken oral health education. 2. As for oral health state perceived by oneself, the response as saying of 'thinking it to be healthy' and that 'there is something wrong' showed the distribution of 34.1% that is the same ratio. As for oral health interest, a case of having a little interest(58.4%) or of being so much interested(12.5%) showed 70.9%. A case of having no special interest(24.8%) or of having no interest at all(4.3%) showed the level of 29.1%.3. As for oral health knowledge, the toothbrushing knowledge was the highest with 4.54 marks out of 6-point perfection. The toothbrush knowledge(4-point perfection) showed low point with 2.05 marks. 4. By gender, a case of men was high in OHIP(Oral Health Impact Profile) for the functional restriction and physical pain. On the other hand, in the remaining sphere, women were indicated to be high in OHIP. The statistically significant difference was indicated in the spheres such as physical pain, psychological discomfort, a drop in psychological ability, a drop in social ability, and social separation. 5. According to oral health state perceived by oneself, a case of thinking it to be healthy was indicated to be high in OHIP for every sphere. A case of thinking it to have gum disease was indicated to be low in OHIP for every sphere. The statistically significant difference was indicated in every sphere except the sphere of functional restriction. 6. As for a factor of having influence upon OHIP according to oral health knowledge, it was indicated that the higher toothbrush knowledge leads to having more influence upon OHIP. Conclusions : It could be known that the oral health knowledge and OHIP are varied as well according to the oral health interest level. Also, the oral health education proper for subjects is not being performed. Even as for frequency of visiting the dentist, a regular visit is failing to be made. In consideration of these points, the diversified and effective educational program is likely considered to be necessarily developed and improved that subjects can have more interest in oral health.

• 한국식품과학회지
• /
• 제27권1호
• /
• pp.124-128
• /
• 1995

결명자 추출물을 이용하여 발암성 니트로사민의 직접적인 생성인자인 아질산염 소거작용과 그 소거인자의 분리 및 동정을 시도하였다. 결명자의 메탄올 추출물을 chloroform, ethyl ether, ethyl acetate, 물분획 순으로 순차용매 분리한 결과 ethyl acetate획분이 가장 우수한 것으로 나타났다. Ethyl acetate획분을 silica gel column chromatography하여 얻은 compound A와 B획분 중에서 compound A는 pH 1.2에서 44.5%/200 ${\mu}g$의 아질산염 소거능을, compound B는 극히 낮은 아질산염 소거능을 나타내었다. HPLC를 이용하여 compound A획분으로 부터 compound A-1획분을 분리하고 아질산염 소거효과를 ascorbic acid와 비교하였을 때 compound A-1은 ascorbic acid에 비해 월등한 소거효과를 나타내었으며, UV, IR 및 $^1H-NMR$의 결과로부터 그 구조는 $nor-rubrofusarin-6-{\beta}-mono-D-glucoside$으로 추정하였다.

• 한국전산유체공학회지
• /
• 제13권4호
• /
• pp.1-7
• /
• 2008

In the process of continuous hot-dip galvanizing, it is well known that the gas wiping through an air knife system is most effective because of its uniformity in coating thickness, possibility of thin coating, workability in high speed, and simplicity of control. However, gas wiping used in the galvanizing process brings about a problem of splashing at the strip edge above a certain high speed of process. It is also known that the problem of edge splashing is more harmful than that at the mid strip surface. For a given liquid(of a certain viscosity and surface tension), the onset of splashing mainly depends upon the strip velocity, the gas-jet pressure, and the nozzle's stand-off distance. In these connections in the present study, we proposed three kinds of air knife system having nozzles of constant expansion rate, and compared the jet structures issuing from newly proposed nozzle systems with the result by a conventional one. In numerical analysis, the governing equations are consisted of two-dimensional time dependent Navier-Stokes equations, and the standard k-${\varepsilon}$ turbulence model is employed to solve turbulence stress and so on. As the result, it is found that we had better use the constant expansion-rate nozzle which can be interpreted from the point view of the energy saving for the same coating thickness. Also, we better reduce the size of separation bubble and enhance the cutting ability at the strip surface, by using an air-knife having constant expansion-rate nozzle.

• 터널과지하공간
• /
• 제19권6호
• /
• pp.534-544
• /
• 2009

지표 하부 공동의 붕괴는 인간 활동의 대상인 도로 및 건물의 안정성에 영향을 미치는 지반침하를 야기할수 있다. 그러므로 이러한 지반침하를 사전에 예방하기 위해서는 지하공동 충전에 의한 지반보강공법의 적용이 필요하다. 본 연구에서는 장기적으로 친환경적인 칼슘알루미네이트 광물을 지반침하보강공법에 적용하고자 급결 충전물질 및 충전시스템, 급경사 광산채굴적 충전 손실 개선에 관한 현장적용성 연구를 수행하였다. 본 연구에서 개발된 급결 경화 충전물질은 급결 성능재료와 보조 재료로 구성된다. 개발된 재료는 급결 성능 및 수중 불분리 특성을 가지고 있어 수중공동에서 재료의 손실을 최소화하면서 효과적으로 지하댐을 형성하였다. Hg, Cd, As, $Cr^{6+}$, Pb와 같은 중금속 용출시험결과, 환경적으로도 적합한 물질로 분석되었다.

• 가족자원경영과 정책
• /
• 제16권1호
• /
• pp.149-175
• /
• 2012

The purpose of this study is to find out marital conflicts and coping strategies among married female immigrants and Korean men in multicultural families. The data were collected by in.depth interviews with 12 female immigrants and 5 Korean men. The major findings of this study are as follows: Female participants suffered from marital conflicts with their spouses, conflicts with mothers.in.law, stereotyped gender roles as a wife and a daughter.in.law, economic conflicts, and cultural differences between Korea and their country. In addition, male participants reported conflicts from economic support for their wife's family, gender role differences, and cultural differences. Coping strategies used to deal with the conflicts, such as mutual dedication and understanding their spouse's stance, and developing a compromise for the family's happiness were mentioned. A personal positive nature and attitude helped them to meet the marital conflicts. Supports from their husband, parents.in.law, and society also helped female immigrants to adapt to cultural differences. However, some of them used negative coping strategies which did not reduce conflicts, and resulted in separation and divorce. The coping strategies to deal with the conflicts depended on the participants' personal ability. While female participants who had English language skills took advantage to adapt to Korean society, those who did not have any other natural abilities could develop a definite position in their family and society with their diligence and positive attitude. The results showed that female participants who were supported by their family and society tended to cope successfully with conflicts and also showed that multicultural families' marital adjustment was maintained with their efforts as well as by relationships with their relatives and society.

• 한국약용작물학회지
• /
• 제27권6호
• /
• pp.427-435
• /
• 2019

Background: A growing interest in health has increased the need for the development of potent antioxidant materials known to play a role in various physiological activities. Currently research and development of non-toxic natural antioxidants with high activity is ongoing. Methods and Results: In this study, we measured 2,2'-azinobis- (3-ethylbenzothiazoline-6-sulfonic acid) (ABTS) and 2,2-diphenyl-1-picrylhydrazyl (DPPH) radical scavenging ability of 10 plant essential oils, selecting samples of Dendranthema indicum, Dendranthema zawadskii, and Citrus sunki essential oils. The samples were analyzed using liquid chromatography (LC) and the radical scavenging activity on LC-based systems with the same conditions. In the LC-mass spectroscopy (MS)/MS analysis of the active compound peak, 2-methoxy-4-vinylphenol with a molecular weight of 150.1 g/mol was identified in C. sunki essential oils. Eugenol or isoeugenol with a molecular weight of 164.1 g/mol as identified in D. indicum and D. zawadskii essential oils as radical scavenging active compounds. Conclusions: In the LC-based measurement system, the active ingredient can be identified by simultaneously conducting profile analysis and the radical scavenging activity of essential oil samples. In addition, LC-MS/MS analysis of the active compound peaks can be performed under the same separation conditions to obtain data that can identify the active compounds in the sample.

• 한국공간구조학회논문집
• /
• 제20권2호
• /
• pp.39-49
• /
• 2020

In this study, we will develop a hybrid cross-sectional shape of steel inserted type glued-laminated timber that can improve the strength of structural glued-laminated timber and maximize the ductility by using steel plate with excellent tensile and deformation ability. A total of three specimens were fabricated and the flexural performance test was carried out to evaluate the structural performance of the steel inserted type glued-laminated timber. In order to compare the effect of steel inserted glued-laminated timber, one structural glued-laminated timber test specimen composed of pure wood was manufactured. In addition, in order to evaluate the adhesion performance of the steel inserted, one each of a screw joint test specimen and a polyurethane joint test specimen was prepared. As a result, all the specimens showed the initial crack in the finger joint near the force point. This has been shown to be a cause of crack diffusion and strength degradation. The use of finger joints in the maximum moment section is considered to affect the strength and ductility of the glued-laminated timber beam. Polyurethane-adhesive steel inserted glued-laminated timber showed fully-composite behavior with little horizontal separation between the steel plate and glued-laminated timber until the maximum load was reached. This method has been shown to exhibit sufficient retention bending performance.

• Clinical and Experimental Reproductive Medicine
• /
• 제23권1호
• /
• pp.81-86
• /
• 1996

The assessment of acrosomal status is important in evaluating the ability of sperm to fertilize the egg. The acrosomal status of sperm from 47 normal volunteers with proven fertility and 167 subfertile men with not to achieve pregnancy for at least 1 year were evaluated with Acrobeads test(FUSO Pharmaceutical Industries, Ltd, Japan) using immunobeads coated with MH61 monoclonal antibody, which is specific for acrosome-reacted sperm. The mean${\pm}$SD of acrobeads score in 47 volunteer group was $2.8{\pm}0.7$, of which 46(97.9%)cases were ${\geq}$ 2. The mean${\pm}$SD of acrobeads score in 167 subfertile group was $1.7{\pm}0.8$, of which 73(79.3%)cases were ${\leq}$ 1. The aerobe ads score in subfertile group were significantly lower(r=0.294, p<0.05) than those in volunteer group. In subfertile group, acrobeads score were well correlated with the sperm density and motility(r=0.275, r=0.281, p<0.01), but not with semen volume(r=0.16) and serum hormone level(FSH r=0.084, LH r=0.036, testosterone r=0.058, prolactin r=0.006 and estradiol r=0.060)(p>0.05). Of 63 subfertile cases with normozoospermia, 22(34.9%)cases showed 0 or 1 of acrobeads score, which means to accompany with a functional defect in spite of normal morphology. As a results, Acrobeads test is not only a technically simple sensitive procedure with good reproducibility in evaluating the sperm fertilizing capacity but also an useful in the evaluation of effectiveness in the treatment of infertility and the separation of acrosome-reacted sperm in the assisted reproductive technique.

• 원예과학기술지
• /
• 제34권2호
• /
• pp.242-248
• /
• 2016

This research was conducted to determine the effects of four different substrates, expanded rice hulls (ERH), commercial substrates for strawberries (CSS), clay sand (CS), and loamy sand (LS), on the inhibition of anthracnose crown rot (ACR) in strawberry. Mother plants of 'Seolhyang' strawberry were transplanted into an elevated bed in March, 2013 and March, 2014 and the runners connecting mother plants and daughter plants were cut in early August of both years. After separation, growth characteristics of the daughter plants were measured and then each daughter plant was inoculated with conidial suspensions of Colletotrichum fructicola, one of several species of Colletotrichum that causes ACR in strawberries. The incidence of ACR as influenced by the different substrates was investigated in both years. The daughter plants grown on CSS had the highest values for shoot height, leaf area, and fresh weight. Those grown on ERH and LS substrates also displayed good above-ground growth characteristics except for fresh weight, but the daughter plants grown on CS had the poorest above-ground growth characteristics. The ERH and CS treatments resulted in the highest number of primary roots and the greatest root weight. The CSS-grown daughter plants had the highest ACR disease index, followed by the CS and LS treatments, but there were no significant differences among the three substrates. However, the ERH-grown daughter plants had a markedly lower ACR disease index on October 11, 2013 and October 7, 2014. The CSS-grown daughter plants had high nitrogen and potassium contents and low calcium content, whereas the ERH-grown daughter plants had low nitrogen levels and high silicon levels. The results of this study provide basic information on the ability of the different substrates tested to provide disease suppression of ACR in the propagation of strawberry transplants.