• 한국식품영양과학회지
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• 제27권4호
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• pp.751-755
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• 1998

Functional properties of yam mucilage were investigated by physicochemical analysis. Yam mucilage was extracted from the root of yam and then separated into two fractions by its selective aggregation with isopropanol concentration. Each mucilge fraction showed the excellent binding properties with heavy metal ions Co, Cr and Cu. Cr showed the higher affinity with mucilage than Co and Cu at pH 6.3. In ACE inhibition, IC50 values of mucilage fraction 1 and 2 showed 8.99$\mu\textrm{g}$/${mu}ell$ and 7.1$\mu\textrm{g}$/${mu}ell$, respectively.

• International Journal of Naval Architecture and Ocean Engineering
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• 제13권1호
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• pp.35-49
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• 2021

This paper presents a direct numerical simulation of turbulent flows over a bump in an open channel to examine the turbulence characteristics near divergent waves emanating from the bump and to investigate the interaction of the turbulences with the divergent waves. To verify the reliability of the simulations, the mean velocity profile and root-mean-square of velocity fluctuations are compared with previous data. The anisotropic invariant maps show that the ratio of the streamwise to spanwise velocity fluctuations plays an important role in characterizing the anisotropic nature of the separated shear layer behind the bump in the vicinity of the free surface. The vortex identification discloses a large-scale streamwise vortical structure from the mean velocity field and a cluster of small coherent structures from the instantaneous velocity field, which are responsible for the anisotropic characteristics of the turbulence beneath the free surface.

• 치과방사선
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• 제27권2호
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• pp.135-144
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• 1997

The primary intra-osseous carcinoma (PIOC) is a very rare lesion. PIOC is an odontogenic carcinoma defined as a squamous cell carcinoma arisinig within a jaw having no initial connection with the oral mucosa, and presumably developing from residues of the odontogenic epithelium. The authors diagnosed a 51-year-old female as primary intra-osseous carcinoma after undergoing clinical, radiological and histological examinations. The characteristics were as followed : 1. The patient complained of gingival bleeding on the premolar area in the left maxilla 2. The conventional radiograms showed a relatively well-defined unilocular radiolucent lesion from the mesial aspect of the upper left canine to the mesial aspect of the upper left 1st molar. The 2nd premolar was separated from the 1st molar and the floor of the maxillary sinus was elevated by the lesion. There was a external root resorption of the upper left canine, the 1st premolar, and the 2nd premolar. 3. On the computed tomograms, the osteolytic bony lesion expanded the cortical plate of the left maxilla and displaced the margin of the left maxillary sinus upwards. But the bony lesion was separated from the maxillary sinus by a bony septum. 4. Bone scintigram with /sup 99m/Tc demonstrated the increased uptake in the left maxilla. Sonograms in the neck area and chest P-A radiogram didn't show any abnormalities. 5. Histologically, the tumor islands infiltrating into the surrounding bone increased in alveolar pattern, composed of the malignant cells, and there was a necrosis in the center of the tumor islands.

• 한국응용곤충학회지
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• 제39권2호
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• pp.123-129
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• 2000

국내 과채류 재배단지의 뿌리혹선충 발생에 관한 조사를 위해 1997년부터 1999년까지 경북 성주군을 중심으로 경기 여주군, 경남 함안군, 충북 청원군 등에서 과채류재배지의 토양을 채집하여 식물기생선충 종류와 밀도 조사, 뿌리혹선충 암컷의 효소표현형에 의한 종 동정을 실시하였다. 경북 성주군의 185개 참외재배 포장 중 99개 포장(53.5%)에서 뿌리혹선충이 검출되었고 나선선충류(Helocotylenchus spp.)는 7개, 둥근꼬리선충류(Aphelechus spp.)는 43개, 환선충류(Criconematid)는 26개 포장에서 검출되었다. 뿌리혹선충 암컷의 Malate dehydrogenase 및 Esterase 등 2가지 효소표현형을 이용하여 한국에 분포하는 주요 4종의 동정이 가능하였다. 효소 표현형을 이용하여 성주군 선남면에서 채집된 13개 시료 중 당콩뿌리혹선충으로 동정된 것이 6포장, 고구마뿌리혹선충 5포장이었으며 2개 포장은 두 종의 혼재하는 것으로 나타났다. 성주군 초전면의 6개 포장 시료 중 4개가 땅콩뿌리혹선충, 1개가 고구마뿌리혹선충으로 동정되었으며 1포장의 뿌리혹선충은 효소표현형이 미동정 종으로 나타났다. 경기도 여주군의 참외재배단지에서는 14개 조사대상 중 당근뿌리혹선충이 11개 포장, 땅콩뿌리혹선충이 3개포장으로 당근뿌리혹선충이 우점종인 것으로 나타났다.

• Journal of Ginseng Research
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• 제32권3호
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• pp.257-263
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• 2008

본 실험은 품종별 종자의 크기가 묘삼의 소질에 미치는 영향을 조사하고 우수한 묘삼생산의 기초자료로 삼고자 수행하 였다. 품종간 종자크기별 개갑율과 생존율을 조사한 결과 개갑율은 품종에서 선운이 가장 우수하였고, 천풍의 개갑율이 가장 불량하였다. 종자크기는 개갑율 과 생존율에 영향을 미치지 못하였다. 경장, 경직경, 엽면적은 종자의 크기가 클수록(4.7 mm 이상) 생육이 우수하였으며 종자의 크기가 작을수록 생육이 불량하여 지상부 생육에 종자의 크기가 영향을 미치는 것으로 확인되었다. 근장, 근직경, 근생체중은 종자의 크기가 큰 것(4.7 mm 이상)이 생육이 우수하였으며, 종자의 크기가 작은 것은 생육이 불량하였다. 종자크기별 이용가능 및 불용묘삼을 조사한 결과 종자의 크기가 클 수 록 이용가능 묘삼의 수량과 본수 증가하는 경향이었다. 품종에서는 연풍, 금풍, 고풍이 지상하부의 생육이 우수하였으며, 천풍은 지상하부의 생육이 낮았다. 사용가능 및 우량묘삼을 생산하기 위하여 4 mm이상의 종자만을 파종하여야 할 것으로 생각된다.

• 한국자원식물학회:학술대회논문집
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• 한국자원식물학회 2021년도 춘계학술대회
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• pp.37-37
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• 2021

Calystegia soldanella L. is a perennial herbaceous halophyte belonging to the convolvulaceae family, which mainly grows in coastal sand dunes in Korea. Shoots and rhizomes are edible, and roots called 'Hyoseon Chogeun' are known to have medicinal effects such as antipyretic, sterilization, and diuretic. In addition, physiological activities of antioxidant, anti-inflammatory, antiviral, antifungal and PTP-1B (protein tyrosine phosphate-1B) inhibition have been reported. In this study, in vitro induction cell lines of C. soldanella L. collected from the coastal sand dunes in Jeju island was redifferentiated into adventitious roots that can be used as medicinal resources. Also the biomass of mass-proliferated adventitious roots using a bioreactor were evaluated. Plants of C. soldanella L. were collected from the crevice of the seashore in the coastal area of Taeheung 2-ri, Namwon-eup, Seogwipo-si. Then, it was separated into leaves, stems, rhizomes, and roots, and surface sterilized with 70% ethyl alcohol and 2% NaOCl (sodium hypochlorite). After washing with sterilized water, each organ section was cultured in Hormone-free MS medium (Murashige & Skoog Medium). As a result, the induction response rates were evaluated at 85% and 55%, respectively, in terms of callus formation and shoot generation in the rhizome segment. In the case of the adventitious roots morphological characteristics induced by single-use treatment of auxin-based plant growth regulators IBA and NAA from redifferentiated shoots were compared. Most efficient adventitious root culture method as a rooting rate, number, length, and biomass proliferation in the bioreactor system was confirmed when treated by culturing in MS salts, Sucrose 30 g·L^-1, and IBA 1mg·L^-1 for 4 weeks. In this study, the medium composition and culture period were confirmed using a bioreactor system to mass-proliferate adventitious roots derived from C. soldanella L. in Jeju island. Also this adventitious root line developed a new medicinal material could increase value of the bio-industry ingredient through quantitative and qualitative screening of phyto-bioactive compounds.

• Journal of Periodontal and Implant Science
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• 제25권3호
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• pp.470-477
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• 1995

Flavonoids from Scutellariae Radix possessed a dual function both as an anti-inflammatory agent and an enhancer of cellular activity in gingival fibroblast. The purpose of this study was to evaluate on the toxicity of ethanolic extract from the root of Scutellariae Radix Georgi and its flavonoids, Wogonin, Baicalein, and Baicalin were isolated and purified by the following method. The crude drug was extracted with ethyl acetate and the residue was dissolved in ethyl alcohol. The ethyl alcohol soluble fraction was separated, concentrated, and then chromatographed on a silica gel column. The acute oral LD 50 in rats was determined for EtOH ex. of Scutellariae Radix and three compounds were evaluated with a single oral gavage at three graded dosage levels. The acute intravenous LD 50 was determined with a single intravenous injection via the jugular vein at three graded dosage levels. Groups of 5 male and 5 female rats, 6 week of age at the start of the study, were fed diets containing 3 graded dosage levels for 14 days. Groups of 5 male and 5 female hamster received O.5ml of the test article at once in a day for 5 days to the buccal cheek pouch for two minutes each. The acute oral LD50 for EtOH ex. of Scutellariae Radix is 1430mg/kg, and for Wogonin 1320mg/kg, for Baicalein 1250mg/kg, for Baicalin 1330mg/kg. The acute intravenous toxicity of EtOH ex. of Scutellariae Radix and its extracts was found to be 27mg/kg body weight No toxic effects were observed in rats fed up to 200mg/kg of EtOH ex. of Scutellariae Radix, Wogonin, Baicalein and Baicalin in the diet for 14 days. The acute Mucouse Membrane LD 50 in hamsters was found to be greater than 100mg/kg. These results suggested that EtOH ex. of Scutellariae Radix and its flavonoids are safe for oral care products using limited amount of extract.

• Journal of Periodontal and Implant Science
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• 제27권2호
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• pp.347-361
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• 1997

The present study investigates the effects of DFDB graft combined with Calcium sulfate membrane on the periodontal wound healing in dehiscence defects of dogs. Following the initiation of general anesthesia by I.V. administration of 30mg/kg of pentobarbital, first premolar was extracted and full-thickness flap was elevated from the second to the fourth premolar. The portion of premolars coronal to the alveolar crest was removed and mesial and distal roots separated to produce single rooted teeth. Exposed root canals were sealed with Caviton and covered completely with flaps sutured. Following the healing period of 12 weeks, the surgical sites were uncovered and $4{\times}4mm$ dehiscence defects were surgically created. Those defects with DFDB graft combined with Calcium sulfate membrane following root planing, were designated as test sites and those with flap surgery-only were designated as controls. 1. No foreign-body reaction or inflammation were observed in either groups. Calcium sulfate was completely resorbed in the test sites. 2. Significantly greater amounts of new cementum was observed in test sites compared with the controls. Significant amounts of functionally orientated collagens were observed in the test sites. 3. New bone formation was observed in significantly greater amounts in test sites. The results suggest that combined graft of DFDB and calcium sulfate is extremely biocompatible with a potential for new bone and cementum formation, and functional alignment of periodontal ligaments.

• Natural Product Sciences
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• 제15권1호
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• pp.1-7
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• 2009

This study was performed to investigate the cell growth inhibitory effect of tissue cultured root of wild Panax ginseng C.A. Mayer (tcwPG). The human stomach carcinoma cell line, MKN 74, was incubated with 70% EtOH extract of tcwPG or Panax ginseng C.A. Mayer (PG) for 24 hrs. tcwPG inhibited cell growth at a concentration of $250{\mu}g/ml$. However, Panax ginseng extract did not inhibit cell growth at the same concentration. We also tested the ethyl acetate and $H_2O$ fractions of tcwPG. The inhibitory effect of the ethyl acetate fraction on cell proliferation in MKN 74 cells was more potent than that of the crude extract, and the inhibitory effect of the $H_2O$ fraction was less than that of the ethyl acetate fraction. When we separated tcwPG into polar and non-polar saponin fractions and then measured cell growth inhibition, the non-polar saponin in tcwPG exhibited cytotoxicity. To compare the effects of tcwPG on various cancer cell lines, we measured cytotoxicity in MKN 74 (stomach cancer cell line), SW 620 (colon cancer cell line) and PC 3 (prostate cancer cell line). All three cell lines showed cell growth inhibition, and the cell growth inhibitory effects were not quite different in the various cell lines. The non-polar saponins of tcwPG arrested PC 3 cells at G1-phase as did Panax ginseng.

• 분석과학
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• 제11권6호
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• pp.478-484
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• 1998

상수리나무(Quercus acutissima, 3년생)의 잎, 줄기, 뿌리, 껍질, 및 열매에 각각 존재하고 있는 polyphenol 성분의 분포를 thin layer chromatography와 column chromatography를 이용하여 조사하였다. 총 25종류의 polyphenol 성분을 분리하였는데, 이들 중 잎에서 15개, 줄기에서 11개, 뿌리와 껍질에서 각각 7개를 분리하였으며, 열매에서는 4개의 성분을 분리하였다. 분리한 polyphenol 성분들 중에는 catechin 성분이 모든 부위에서 비교적 많이 분포하고 있었으며 일부 flavonoid 성분들도 확인되었다. 잎을 제외한 모든 부위에서 3종류의 polyphenol isomer의 존재를 확인했으며, 잎에서는 monomer와 oligomer성 polyphenol의 존재를 확인했으나 polymeric polyphenol이 확인되지 않았다. Quercus acutissima에서 polyphenol의 총함량은 껍질에서 상대적으로 가장 많았고, 다음으로는 뿌리, 잎, 열매의 순으로 많이 분포하였으며, 줄기에서 가장 적은 양이 확인되었다.