Browse > Article

Cell Growth Inhibitory Effect of Tissue Cultured Root of Wild Panax ginseng C.A. Mayer Extract on Various Cancer Cell Lines  

Park, Jeong-Sook (College of Pharmacy, Chungbuk National University)
Lee, Tae-Woong (College of Pharmacy, Chungbuk National University)
Han, Kun (College of Pharmacy, Chungbuk National University)
Publication Information
Natural Product Sciences / v.15, no.1, 2009 , pp. 1-7 More about this Journal
Abstract
This study was performed to investigate the cell growth inhibitory effect of tissue cultured root of wild Panax ginseng C.A. Mayer (tcwPG). The human stomach carcinoma cell line, MKN 74, was incubated with 70% EtOH extract of tcwPG or Panax ginseng C.A. Mayer (PG) for 24 hrs. tcwPG inhibited cell growth at a concentration of $250{\mu}g/ml$. However, Panax ginseng extract did not inhibit cell growth at the same concentration. We also tested the ethyl acetate and $H_2O$ fractions of tcwPG. The inhibitory effect of the ethyl acetate fraction on cell proliferation in MKN 74 cells was more potent than that of the crude extract, and the inhibitory effect of the $H_2O$ fraction was less than that of the ethyl acetate fraction. When we separated tcwPG into polar and non-polar saponin fractions and then measured cell growth inhibition, the non-polar saponin in tcwPG exhibited cytotoxicity. To compare the effects of tcwPG on various cancer cell lines, we measured cytotoxicity in MKN 74 (stomach cancer cell line), SW 620 (colon cancer cell line) and PC 3 (prostate cancer cell line). All three cell lines showed cell growth inhibition, and the cell growth inhibitory effects were not quite different in the various cell lines. The non-polar saponins of tcwPG arrested PC 3 cells at G1-phase as did Panax ginseng.
Keywords
Tissue culture; Panax ginseng; Cytotoxicity; MKN 74; SW 620; PC 3;
Citations & Related Records
Times Cited By KSCI : 1  (Citation Analysis)
Times Cited By SCOPUS : 0
연도 인용수 순위
1 Khalil,W.K., Ahmed, K.A., Park, M.H., Kim, Y.T., Park, H.H., andAbdel-Wahhab, M.A., The inhibitory effects of garlic and Panaxginseng extract standardized with ginsenoside Rg3 on thegenotoxicity, biochemical, and histological changes induced byethylenediaminetetraacetic acid in male rats. Arch. Toxicol., 82, 183-95 (2008)   DOI   ScienceOn
2 Kyle, E., Neckers, L., Takimoto, C., Curt, G., and Bergan, R., Genisteininducedapoptosis of prostate cancer cells is preceded by a specificdecrease in focal adhesion kinase activity. Mol. Pharmacol., 51, 193-200 (1997)   PUBMED   ScienceOn
3 Wakabayashi, C., Murakami, K., Hasegawa, H., Murata, J., and Saiki, I.,An intestinal bacterial metabolite of ginseng protopanaxadiol saponinshas the ability to induce apoptosis in tumor cells. Biochem. Biophys.Res. Comm., 246, 725-730 (1998)   DOI   ScienceOn
4 Yue, P.Y.K., Wong, D.Y.L., Wua, P.K., Leung, P.Y., Mak, N.K., Yeung,H.W., Liu, L., Cai, Z., Jiang, Z.H., Fan, T.P.D., and Wong, R.N.S., Theangiosuppressive effects of 20(R)- ginsenoside Rg3. Biochem.Pharmacol., 72, 437-445 (2006)   DOI   ScienceOn
5 Oshima, Y., Sato, K., and Hikino, H., Isolation and hypoglycemic activityof quinquefolans A, B, and C, glycans of Panax quinquefolium roots.J. Nat. Prod., 50, 188-190 (1987)   DOI   PUBMED
6 Matsuda, H., Samukawa, K., and Kubo, M., Anti-inflammatory activity ofginsensoside Ro. Planta Med., 56, 19-23 (1990)   DOI   ScienceOn
7 Matsuda, H., Samukawa, K., and Kubo, M., Anti-hepatic activity ofginsenoside Ro. Planta Med., 57, 523-526 (1991)   DOI   ScienceOn
8 Scaglione, F., Cattaneo, G., Alessandria, M., and Cogo, R., Efficacy andsafety of standardised ginseng extract G115 for potentiatingvaccination against the influenza syndrome and protection against thecommon cold. Drugs Exp. Clin. Res., 22, 65-72 (1996)   PUBMED   ScienceOn
9 Liu, W.K., Xu, S.X., Che, C.T., Anti-proliferative effect of ginsengsaponins on human prostate cancer cell line. Life Sci., 67, 1297-1306(2000)   DOI   ScienceOn
10 Yun, T.K., Panax ginseng -a non-organ-specific cancer preventive?Lancet, 2, 49-55 (2001)   DOI   PUBMED   ScienceOn
11 Keum, Y.S., Park, K.K., Lee, J.M., Chun, K.S, Park, J.H., Lee, S.K.,Kwon, H.J., and Surh, Y.J., Antioxidant and anti-tumor promotingactivities of the methanol extract of heat-processed ginseng. CancerLet.,150, 41-48 (2000)
12 Furuya, T. and Yoshikaya, T., Saponin production by cultures of Panaxginseng transformed with Agrobacterium rhizogenes. Plant Cell Rep.,6, 449-453 (1987)   DOI   ScienceOn
13 Moon, J.A., Yu, S.J., Kim, H.S., and Sohn, J.W., Induction of G1 cellcycle arrest and p27KIP1 increase by panaxydol isolated from Panaxginseng. Biochem. Pharmacol., 59, 1109-1116 (2000)   DOI   ScienceOn
14 Zuin, M., Battezzati, P.M., Camisasca, M., Riebenfeld, D., and Podda, M.,Effects of a preparation containing a standardised ginseng extractcombined with trace elements and multivitamins against hepatotoxininducedchronic liver disease in the elderly. J. Int. Med. Res., 15, 276-281 (1987)   PUBMED   ScienceOn
15 Court, W.A., Hendel, J.G., and Elmi, J., Reversed-phase high-performanceliquid chromatographic determination of ginsenosides of Panaxquinquefolium. J. Chromatogr. A, 755, 11-17 (1996)   DOI   ScienceOn
16 Hahn, E.J., Kim, Y.S., Yu, K.W., Jeong, C.S., and Paek, K.Y.,Adventitious root cultures of Panax ginseng C.A. Meyer andginsenoside production through bioreactor system. J. Plant.Biotechnol., 5, 1-6 (2003)   ScienceOn
17 Yun, T.K. and Choi, S.Y., Non-organ specific cancer prevention byginseng: a study in Korea. Int. J. Epidemiol., 27, 359-364 (1998)   DOI   ScienceOn
18 Lee, S.J., Ko, W.G., Kim, J.H., Sung, J.H., Lee, S.J., Moon, C.K., andLee, B.H., Induction of apoptosis by a novel intestinal metabolite ofginseng saponin via cytochrome c-mediated activation of caspase-3protease. Biochem. Pharmacol., 60, 677-685 (2000)   DOI   ScienceOn
19 Ohnishi, Y., Tagaki, S., and Miura, T., Effect of ginseng radix on GLUT2protein content in mouse liver in normal and epinephrine-inducedhyperglycemic mice. Biol. Pharm. Bull., 19, 1238-1240 (1996)   DOI   PUBMED   ScienceOn