• Parasites, Hosts and Diseases
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• v.57 no.1
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• pp.9-15
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• 2019

Melting temperature shift ($T_m-shift$) is a new detection method that analyze the melting curve on real-time PCR thermocycler using SYBR Green I fluorescent dye. To establish a $T_m-shift$ method for the detection of Ancylostoma ceylanicum and A. tubaeforme in cats, specific primers, with GC tail of unequal length attached to their 5' end, were designed based on 2 SNP loci (ITS101 and ITS296) of the internal transcribed spacer 1 (ITS1) sequences. The standard curve of $T_m-shift$ was established using the standard plasmids of A. ceylanicum (AceP) and A. tubaeforme (AtuP). The $T_m-shift$ method stability, sensitivity, and accuracy were tested with reference to the standard curve, and clinical fecal samples were also examined. The results demonstrated that the 2 sets of primers based on the 2 SNPs could accurately distinguish between A. ceylanicum and A. tubaeforme. The coefficient of variation (CV) of $T_m$- values of AceP and AtuP was 0.07% and 0.06% in ITS101 and was 0.06% and 0.08% in ITS296, respectively. The minimum detectable DNA concentration was $5.22{\times}10^{-6}$ and $5.28{\times}10^{-6}ng/{\mu}l$ samples of AceP and AtuP, respectively. The accuracy of $T_m-shift$ method reached 100% based on examination of 10 hookworm DNA samples with known species. In the clinical detection of hookworm in 69 stray cat fecal sample, the $T_m-shift$ detection results were consistent with the microscopic examination and successfully differentiated between the 2-hookworm species. In conclusion, the developed method is a rapid, sensitive and accurate technique and can provide a promising tool for clinical detection and epidemiological investigation of cat-derived hookworms.

• Journal of the Computational Structural Engineering Institute of Korea
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• v.31 no.6
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• pp.353-358
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• 2018

In this study, it is describe to an optimization analysis process for the weight reduction of the voltage converter in the electric vehicle charging systems. The optimization design is a technique that finds the optimal material distribution under a given material quantity constraint by combining the design sensitivity with the material properties and the mathematical optimization. Among the topology optimization, a lightweight design is performed by a solid isotropic material with penalization with simple formula and well-convergence. The lightweight design consists of three steps. As a first step, a finite element model for the basic design of the on-board voltage converter was constructed and static analysis was performed on the load. In the second step, the optimum shape is obtained for the lightweight by performing the topology optimization using the solid isotropic material with penalization applying the stiffness coefficient of the isotropic material to the static analysis result. As a final step, impact analysis was performed by applying a half-sinusoidal pulse shape impact load which satisfies the impact test standard of the vehicle-mounted part with respect to the optimum shape. In the topology optimization, the design domain was defined as the mounting bracket area, and the design technology was finally achieved by optimizing the mounting bracket to achieve a weight reduction of 20% over the basic design.

• Proceedings of the Korea Water Resources Association Conference
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• 2015.05a
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• pp.217-217
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• 2015

The objective of this study was mainly to evaluate the water resources potential of Lake Tana Basin (LTB) by using Soil and Water Assessment Tool (SWAT). From SWAT simulation of LTB, about 5236 km2 area of LTB is gauged watershed and the remaining 9878 km2 area is ungauged watershed. For calibration of model parameters, four gauged stations were considered namely: Gilgel Abay, Gummera, Rib, and Megech. The SWAT-CUP built-in techniques, particle swarm optimization (PSO) and generalized likelihood uncertainty estimation (GLUE) method was used for calibration of model parameters and PSO method were selected for the study based on its performance results in four gauging stations. However the level of sensitivity of flow parameters differ from catchment to catchment, the curve number (CN2) has been found the most sensitive parameters in all gauged catchments. To facilitate the transfer of data from gauged catchments to ungauged catchments, clustering of hydrologic response units (HRUs) were done based on physical similarity measured between gauged and ungauged catchment attributes. From SWAT land use/ soil use/slope reclassification of LTB, a total of 142 HRUs were identified and these HRUs are clustered in to 39 similar hydrologic groups. In order to transfer the optimized model parameters from gauged to ungauged catchments based on these clustered hydrologic groups, this study evaluates three parameter transfer schemes: parameters transfer based on homogeneous regions (PT-I), parameter transfer based on global averaging (PT-II), and parameter transfer by considering Gilgel Abay catchment as a representative catchment (PT-III) since its model performance values are better than the other three gauged catchments. The performance of these parameter transfer approach was evaluated based on values of Nash-Sutcliffe efficiency (NSE) and coefficient of determination (R2). The computed NSE values was found to be 0.71, 0.58, and 0.31 for PT-I, PT-II and PT-III respectively and the computed R2 values was found to be 0.93, 0.82, and 0.95 for PT-I, PT-II, and PT-III respectively. Based on the performance evaluation criteria, PT-I were selected for modelling ungauged catchments by transferring optimized model parameters from gauged catchment. From the model result, yearly average stream flow for all homogeneous regions was found 29.54 m3/s, 112.92 m3/s, and 130.10 m3/s for time period (1989 - 2005) for region-I, region-II, and region-III respectively.

• Korean Journal of Agricultural Science
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• v.48 no.2
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• pp.299-310
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• 2021

Glycerol is a non-volatile compound with no aromatic properties that contributes significantly to the quality of wine by providing sweetness and richness of taste. In addition, it is also the third most significant byproduct of alcoholic fermentation in terms of quantity after ethanol and carbon dioxide. In this study, Fourier transform infrared (FT-IR) spectroscopy was employed as a fast non-destructive method in conjugation with multivariate regression analysis to build a model for the quantitative analysis of glycerol concentration in wine samples. The samples were prepared by using three varieties of red wine samples (i.e., Shiraz, Merlot, and Barbaresco) that were adulterated with glycerol in concentration ranges from 0.1 to 15% (v·v^-1), and subjected to analysis together with pure wine samples. A net analyte signal (NAS)-based methodology, called hybrid linear analysis in the literature (HLA/GO), was applied for predicting glycerol concentrations in the collected FT-IR spectral data. Calibration and validation sets were designed to evaluate the performance of the multivariate method. The obtained results exhibited a high coefficient of determination (R²) of 0.987 and a low root mean square error (RMSE) of 0.563% for the calibration set, and a R² of 0.984 and a RMSE of 0.626% for the validation set. Further, the model was validated in terms of sensitivity, selectivity, and limits of detection and quantification, and the results confirmed that this model can be used in most applications, as well as for quality assurance.

• Journal of Korean Society of Coastal and Ocean Engineers
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• v.32 no.6
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• pp.373-383
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• 2020

In this study we established an operational storm-surge system for the northwestern pacific ocean, based on the NEMO (Nucleus for European Modeling of the Ocean). The system consists of the tide and the surge models. For more accurate storm surge prediction, it can be completed not only by applying more precise depth data, but also by optimal parameterization at the boundaries of the atmosphere and ocean. To this end, we conducted several sensitivity experiments related to the application of available bathymetry data, ocean bottom friction coefficient, and wind stress and air pressure on the ocean surface during August~September 2018 and the case of typhoon SOULIK. The results of comparison and verification are presented here, and they are compared with POM (Princeton Ocean Model) based Regional Tide Surge forecasting Model (RTSM). The results showed that the RTSM_NEMO model had a 29% and 20% decrease in Bias and RMSE respectively compared to the RTSM_POM model, and that the RTSM_NEMO model had a lower overall error than the RTSM_POM model for the case of typhoon SOULIK.

• Journal of Biomedical Engineering Research
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• v.43 no.1
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• pp.19-26
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• 2022

The Comprehensive in vitro Proarrhythmic Assay(CiPA) project was launched for solving the hERG assay problem of being classified as high-risk groups even though they are low-risk drugs due to their high sensitivity. CiPA presented a protocol to predict drug toxicity using physiological data calculated based on the in-silico model. in this study, features calculated through the in-silico model are analyzed for correlation of changing action potential in the near future, and features are verified through predictive performance according to drug datasets. Using the O'Hara Rudy model modified by Dutta et al., Pearson correlation analysis was performed between 13 features(dVm/dtmax, APpeak, APresting, APD90, APD50, APDtri, Ca_peak, Ca_resting, CaD90, CaD50, CaDtri, qNet, qInward) calculated at 100 pacing, and between dVm/d_{tmax_repol} calculated at 1,000 pacing, and linear regression analysis was performed on each of the 12 training drugs, 16 verification drugs, and 28 drugs. Indicators showing high coefficient of determination(R²) in the training drug dataset were qNet 0.93, AP resting 0.83, APDtri 0.78, Ca resting 0.76, dVm/dt_max 0.63, and APD90 0.61. The indicators showing high determinants in the validated drug dataset were APDtri 0.94, APD90 0.92, APD50 0.85, CaD50 0.84, qNet 0.76, and CaD90 0.64. Indicators with high coefficients of determination for all 28 drugs are qNet 0.78, APD90 0.74, and qInward 0.59. The indicators vary in predictive performance depending on the drug dataset, and qNet showed the same high performance of 0.7 or more on the training drug dataset, the verified drug dataset, and the entire drug dataset.

• Journal of the Korean Society of Marine Environment & Safety
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• v.28 no.6
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• pp.1044-1053
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• 2022

Time domain reflectometry (TDR) is a diagnostic technique to evaluate the physical integrity of cable and finds application in leak detection and localization of piping system. In this study, a cable-shaped leak detection sensor was proposed using the TDR technique for monitoring leakage detection of ship's engine room seawater piping system. The cable sensor was developed using a twisted pair arrangement and wound by an absorbent material. The availability and performance of the sensor for leak detection and localization were evaluated on a lab-scale pipeline set up. The developed sensor was installed onto the pipes and flanges of the lab-scale set up and various TDR waveforms were acquired and analyzed according to the dif erent variables including the number of twists and sheath thickness. The result indicated that the twisted cable sensor was able to produce clear and smooth signal as compared to the TDR sensor with a parallel arrangement. The optimal number of twist was determined to be above 10 per the unit length. The optimal diameter of sheath thickness that results in the desired sensitivity was determined to be ranging from 80% up to 120% of the diameter of the conductor. The linear regression analysis for estimation of leak localization was carried out to estimate the location of the leakage, and the result was a determination coefficient of 0.9998, indicating a positive relationship with the actual leakage point. The proposed TDR based leak detection method appears to be an effective method for monitoring leakage of ship's seawater piping system.

• The Journal of the Acoustical Society of Korea
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• v.41 no.3
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• pp.278-286
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• 2022

In this study, a 33-mode Free-Flooded Ring (FFR) transducer was designed to apply piezoelectric single crystal PIN-PMN-PT, which has high piezoelectric constants and electromechanical coupling coefficient. To ensure low-frequency high transmitting sensitivity characteristics with a small size of FFR transducer, the commercial FFR transducer based on piezoelectric ceramics was compared. To develop the FFR transducer with broadband characteristics, a piezoelectric segmented ring structure inserted with inactive elements was applied. The oil-filled structure was applied to minimize the change of acoustic characteristics of the ring transducer. It was verified that the transmitting voltage response, underwater impedance, and beam pattern matched the finite element numerical simulation results well through an acoustic test. The difference in the transmitting voltage response between the measured and the simulated results is about 1.3 dB in cavity mode and about 0.3 dB in radial mode. The fabricated FFR transducer had a higher transmitting voltage response compared to the commercial transducer, but the diameter was reduced by about 17 %. From this study, it was confirmed that the feasibility of a single crystal-applied FFR transducer with compact size and high-power characteristics. The effectiveness of the performance prediction by simulation was also confirmed.

• Journal of the Korea institute for structural maintenance and inspection
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• v.27 no.4
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• pp.23-32
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• 2023

The seismic performance of buried PE pipes is reported to be favorable due to their exceptional elongation capacity at break. Although a seismic performance evaluation procedure based on the response displacement method has been summarized in Korea for fusion-bonded PE pipes, there is currently no procedure available for mechanically jointed PE pipes. This article aims to present a seismic performance evaluation procedure based on the response displacement method specifically designed for mechanically jointed PE pipes in Korea. When employing the mechanical joining method for PE pipes, it is recommended to adhere to the evaluation procedure established for segment-type pipes. This involves assessing the stress induced by the pipe, the expansion and contraction strain of the joint, and the bending angle of the pipe joint. Furthermore, the coefficient of inhomogeneity of the soil, which is necessary for estimating the axial strain of the ground, is introduced. Additionally, a computation method for determining lateral displacement and reconsolidation settlement in soil susceptible to liquefaction is proposed. As a result of the sensitivity analysis considering the typical soil condition in Korea, the mechanically jointed PE pipe with a certain quality was shown to have good structural seismic safety when soil liquefaction was not considered. This procedure serves as a valuable tool for seismic design and evaluating the seismic performance of mechanically joined buried PE pipes, which are primarily utilized for connecting small-diameter pipes.

• Journal of Korean Clinical Nursing Research
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• v.29 no.2
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• pp.198-209
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• 2023

Purpose: The purpose of this study was to develop a Korean Adult Patients Delirium Screening Tool (K-APDS) for those admitted to general wards, and to verify its reliability and validity. Methods: For the development of the tool, 12 items were derived through the results of literature review and focus group interviews with general ward nurses, and the content validity was confirmed by experts. To verify the reliability and validity of the developed tool, 317 adult patients who were admitted to general wards of three tertiary general hospitals from October to November 2022 were evaluated by the attending nurse and data were collected. Results: After factor analysis for construct validity verification, two factors were extracted, which explained 60.1% of the total variance. After the validation of the control group, the difference in the delirium incidence scores calculated using the K-APDS between the delirium group and non-delirium group was very significant (Z=-10.82, p<.001). To verify the criterion validity, K-APDS, Delirium Observation Screening, and Pearson's correlation coefficient were checked and found to be .94 (p<.001). The predictive validity test reported that the sensitivity was 91.1%, specificity was 82.4%, positive predictive value was 52.6%, and negative predictive value was 97.8%. The reliability of K-APDS was found to be high with Cronbach's ⍺=.91. Conclusion: K-APDS can screen for delirium with 2 or more points, excellent validity and reliability have been verified. Therefore, this tool could be applied immediately in the clinical field, and will contribute to the early detection of delirium, enabling rapid interventions.