• 한국전자파학회논문지
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• 제15권11호
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• pp.1100-1107
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• 2004

본 논문에서는 다단 결합선로 여파기(edge coupled-line filter)의 입출력단을 탭 선로(tapped-line)의 형태로 구현하기 위하여 등가회로 기반의 새로운 설계법을 개발하여 적용하였다. 다단 결합선로 여파기는 입출력단의 선로 간격이 매우 좁아서(수 mil) 제작하기가 힘든 결함이 있는데, 이를 탭 선로로 대체함으로써 제작 시 오차에 덜 민감하고 밀링머신으로 쉽게 제작할 수 있도록 하였다. 또한, 본 논문에서 제안된 간단한 설계법을 사용하면 20 GHz 이하의 주파수 대역에서 20 % 이하의 대역폭 특성을 갖는 탭 선로의 파라미터를 얻을 수 있으며, 이를 바탕으로 정확한 탭 선로 여파기를 설계할 수 있다.

• 한국응용약물학회:학술대회논문집
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• 한국응용약물학회 1994년도 춘계학술대회 and 제3회 신약개발 연구발표회
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• pp.129-130
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• 1994

The science of toxicology is the understanding of the mechanisms by which exogenous agents produce deleterious effects in biological systems. The actions of chemicals such as drugs are ultimately exerted at the cellular and gene levels. Over the past decade. several in vitro alternative methods such as cultured cells for assessing the toxicity of various xenobiotics have been proposed to reduce the use of animals. In this workshop three advanced methods will be presented. These methods are novel important models for toxicologic studies. Dr. Tabuchis group has establishcd two immortalized gastric surface mucosa cell lines from the pminary cultore of gastric fundic mucosal cells of adult transgenic mice harboring a temperature sensitive simian virus 40 large T-anugen gene. As the immortalized cell lines of various tissues possess unique characteristics to maintain their normal functions for several months, these cell lines are extremely useful for not only toxicity testing but also pharmacological screening in new drug development. Professor Funatsu have studied the formation of spherical multicelluar aggregates of adult rat hepatocytes(spheroid) having tissue like structure. The sphcroid shown thre is a prototype module of an artificial liver support system. Thus, the urea synthesis activity of the artificial liver was maintained at least to days in 100％ rat blood plasma. Dr. Takezawa and his coworkers have developed a novel culture system of multicellular spheroids considered 〃organoids〃 by utilizing a thermo-responsive polymer as a substratum of anchorage dependent cells. His final goal is to reconstitute the organoids of various normal organs, e.g., liver, skin etc. and also abnormal deseased organs such as tumor.

• Journal of Life Science
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• 제9권1호
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• pp.40-44
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• 1999

Glutathione S-transferase (GST) is a multifunctional protein that catalyzes the catalyzes the conjugation of glutathione with electrophilic compounds. It exists in a variety of isoenzy-matic froms with a wide range of substrate specificity and plays a pivotal role in detoxification of various drugs. In order to elucidate the GST-${\pi}$'s involvement of multidrug resistance (MDR) in drug-resistant tumor cell lines, we determined GST-${\pi}$ content by "1 step sandwich method". Consequently, adriamycin resistant cells of MCF-7 (MCF-7/ADM) have 7-fold increase of GST-${\pi}$ content than that of MCF-7 cells, while its {TEX}$IC_{50}${/TEX} was 116-fold greater than parent cell line. By northrn blotting, we compared whether MCF-7/ADM cells express GST-${\pi}$ mRNA. The GST-${\pi}$ mRNA expression in these cells was not inducible, but constitutive when treated for 24 h with a concentration of 0, 20, 200, and 2000 nM of adriamycin, respectively. Taken together, these results suggest that GST-${\pi}$ may not be directly associated with multidrug resistance in these human cancer cell lines.ell lines.

• 치과방사선
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• 제24권2호
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• pp.249-260
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• 1994

The purpose of this study was to aid in the radiation therapy of head and neck cancer patients. For this study, radiation survival curves were generated for Bl6, MG-63 and YAC-l cell lines using semiautomated MTT assay and Dye Exclusion Assay. Irradiation of 2, 4, 6, 8, 10Gy were delivered at room temperature at a dose rate of 210.2cGy/min using / sup 60/Co γ-ray Irradiator ALOORAOO 8. The viable cells were determined for each radiation dose and compared to control values. The obtained results were as follows: 1. There was significantly different absorbance at 10Gy on B16 cell line in MTT assay(P<0.05). 2. There was significantly different absorbance at 4, 6, 8, 10Gy on MG-63 cell line in MTT assay(P<0.05). 3. YAC-l cell line was more sensitive than B16 or MG-63 cell line to all doses of radiation(P<0.05). 4. There was significantly different absorbance among all tumor cell lines except between B16 and MG-63 cell line at ZGy in MTT assay(P<0.05). 5. Good correlation was obtained between MTT assay and DEA(P<0.05). The efficient of correlation of B16, MG-63 and YAC-l cell line was 0.845, 0.824 and 0.906, respectively.

• 대한원격탐사학회지
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• 제34권1호
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• pp.17-24
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• 2018

Grounding line of a glacier or ice shelf where ice bottom meets the ocean is sensitive to changes in the polar environment. Recent rapid changes of grounding lines have been observed especially in southwestern Antarctica due to global warming. In this study, ERS-1/2 and Sentinel-1A Synthetic Aperture Radar (SAR) image were interferometrically acquired in 1996 and 2015, respectively, to monitor the movement of the grounding line in the western part of Ronne Ice Shelf near the Antarctic peninsula. Double-Differential Interferometric SAR (DDInSAR) technique was applied to remove gravitational flow signal to detect grounding line from the interferometric phase due to the vertical displacement of the tide. The result showed that ERS-1/2 grounding lines are almost consistent with those from Rignot et al. (2011) which used the similar dataset, confirming the credibility of the data processing. The comparison of ERS-1/2 and Sentinle-1A DDInSAR images showed a grounding line retreat of $1.0{\pm}0.1km$ from 1996 to 2015. It is also proved that the grounding lines based on the 2004 MODIS Mosaic of Antarctica (MOA) images and digital elevation model searching for ice plain near coastal area (Scambos et al., 2017), is not accurate enough especially where there is a ice plain with no tidal motion.

• Journal of Applied Biological Chemistry
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• 제56권1호
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• pp.49-51
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• 2013

In a previous study, we reported that 13(E)-labd-13-ene-$8{\alpha}$,15-diol (13E) possesses antiviral and anticancer activities. In this study, the anticancer and antimicrobial activities of 13(E) were evaluated against 4 cancer cell lines and 6 bacteria. 13(E) showed inhibitory effect on a variety of cancer cell lines. The $IC_{50}$ values was 8.3-21.3 ${\mu}g/mL$. 13(E) was the most effective growth inhibitor of murine leukaemia cell lines P388, producing approximately 8.3 ${\mu}g/mL$ of $IC_{50}$ in the cytopathic effect (CPE) method. 13(E) also inhibited the growth of the gram-positive bacteria (Staphylococcus aureus, Bacillus cereus and Listeria monocytogenes) and gram-negative bacteria (Vibrio parahaemolyticus, Escherichia coli and Salmonella enteritidis) with a range of minimum inhibitory concentration (MIC) values from 0.092 to 0.598 mg/mL and gram-negative bacteria were more sensitive to the compound (MIC, 0.092 mg/mL).

• 천문학회지
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• 제55권2호
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• pp.59-66
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• 2022

Stellar magnetic activity is important for formulating the evolution of the star. To represent the stellar magnetic activity, the S index is defined using the Ca II H+K flux measure from the Mount Wilson Observatory. Mg II lines are generated in a manner similar to the formation of Ca II lines, which are more sensitive to weak chromospheric activity. Mg II flux data are available from the International Ultraviolet Explorer (IUE). Thus, the main purpose of this study was to analyze the magnetic activity of stars. We used 343 high-resolution IUE spectra of 14 main-sequence G stars to obtain the Mg II continuum surface flux and Mg II line-core flux around 2,800 Å. We calculated S index using the IUE spectra and compared it with the conventional Mount Wilson S index. We found a color (B - V ) dependent association between the S index and the Mg II emission line-core flux. Furthermore, we attempted to obtain the magnetic activity cycles of these stars based on the new S index. Unfortunately, this was not successful because the IUE observation interval of approximately 17 years is too short to estimate the magnetic activity cycles of G-type stars, whose cycles may be longer than the 11 year mean activity cycle of the sun.

• Journal of Microbiology and Biotechnology
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• 제25권7호
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• pp.978-987
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• 2015

Aluminum (Al) toxicity is one of the greatest limitations to agriculture in acid soils, particularly in tropical regions. Arbuscular mycorrhizal fungi (AMF) can supply plants with nutrients and give protection against Al toxicity. The aim of this work was to evaluate the effects of soil liming (i.e., reducing Al saturation) on the AMF community composition and structure in the roots of maize lines contrasting for Al tolerance. To this end, we constructed four 18S rDNA cloning libraries from L3 (Al tolerant) and L22 (Al sensitive) maize lines grown in limed and non-limed soils. A total of 790 clones were sequenced, 69% belonging to the Glomeromycota phylum. The remaining sequences were from Ascomycota, which were more prominent in the limed soil, mainly in the L3 line. The most abundant AM fungal clones were related to the family Glomeraceae represented by the genera uncultured Glomus followed by Rhizophagus and Funneliformis. However, the most abundant operational taxonomic units with 27% of the Glomeromycota clones was affiliated to genus Racocetra. This genus was present in all the four libraries, but it was predominant in the non-limed soils, suggesting that Racocetra is tolerant to Al toxicity. Similarly, Acaulospora and Rhizophagus were also present mostly in both lines in non-limed soils. The community richness of AMF in the non-limed soils was higher than the limed soil for both lines. The results suggest that the soil Al saturation was the parameter that mostly influences the AMF species composition in the soils in this study.

• Journal of Astronomy and Space Sciences
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• 제34권2호
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• pp.161-170
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• 2017

In this study, the characteristics of a horizontal sundial from the Joseon Dynasty were investigated. Korea's Treasure No. 840 (T840) is a Western-style horizontal sundial where hour-lines and solar-term-lines are engraved. The inscription of this sundial indicates that the latitude (altitude of the north celestial pole) is $37^{\circ}$ 39', but the gnomon is lost. In the present study, the latitude of the sundial and the length of the gnomon were estimated based only on the hour-lines and solar-term-lines of the horizontal sundial. When statistically calculated from the convergent point obtained by extending the hour-lines, the latitude of this sundial was $37^{\circ}$ $15^{\prime}{\pm}26^{\prime}$, which showed a 24' difference from the record of the inscription. When it was also assumed that a convergent point is changeable, the estimation of the sundial's latitude was found to be sensitive to the variation of this point. This study found that T840 used a vertical gnomon, that is, perpendicular to the horizontal plane, rather than an inclined triangular gnomon, and a horn-shaped mark like a vertical gnomon is cut on its surface. The length of the gnomon engraved on the artifact was 43.1 mm, and in the present study was statistically calculated as $43.7{\pm}0.7mm$. In addition, the position of the gnomon according to the original inscription and our calculation showed an error of 0.3 mm.

• 천문학회보
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• 제37권2호
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• pp.110.2-110.2
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• 2012

We investigate a cool loop and a dark lane over a limb active region on 2007 March 14 by the Hinode/EUV Imaging Spectrometer (EIS). The cool loop is clearly seen in the EIS spectral lines formed at the transition region temperature (log T = 5.8). The dark lane is characterized by an elongated faint structure in coronal spectral lines (log T = 5.8 - 6.1) and rooted on a bright point. We examine their electron densities, Doppler velocities, and non-thermal velocities as a function of distance from the limb using the spectral lines formed at different temperatures (log T = 5.4 - 6.4). The electron densities of the cool loop and the dark lane are derived from the density sensitive line pairs of Mg VII, Fe XII, and Fe XIV spectra. Under the hydrostatic equilibrium and isothermal assumption, we determine their temperatures from the density scale height. Comparing the scale height temperatures to the peak formation temperatures of the spectral lines, we note that the scale height temperature of the cool loop is consistent with a peak formation temperature of the Mg VII (log T = 5.8) and the scale height temperature of the dark lane is close to a peak formation temperature of the Fe XII and Fe XIII (log T = 6.1 - 6.2). It is interesting to note that the structures of the cool loop and the dark lane are most visible in these temperature lines. While the non-thermal velocity in the cool loop slightly decreases (less than 7 km $s^{-1}$) along the loop, that in the dark lane sharply falls off with height. The variation of non-thermal velocity with height in the cool loop and the dark lane is contrast to that in off-limb polar coronal holes which are considered as source of the fast solar wind. Such a decrease in the non-thermal velocity may be explained by wave damping near the solar surface or turbulence due to magnetic reconnection near the bright point.