Journal of Korean Academy of Oral and Maxillofacial Radiology (치과방사선)

Korean Academy of Oral and Maxillofacial Radiology (대한영상치의학회)
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AN EXPERIMENTAL STUDY ON THE LOW-DOSE RADIOSENSITIVITY OF TUMOR CELL LINES

수종의 암세포주의 저선량 방사선감수성에 관한 실험적 연구

  • Kim Min-Sook (Department of Oral and Maxillofacial Radiology, College of Dentistry, Chonbuk National University) ;
  • Koh Kwang-Joon (Department of Oral and Maxillofacial Radiology, College of Dentistry, Chonbuk National University)
  • 김민숙 (전북대학교 치과대학 구강악안면방사선학 교실) ;
  • 고광준 (전북대학교 치과대학 구강악안면방사선학 교실)
  • Published : 1994.12.01
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Abstract

The purpose of this study was to aid in the radiation therapy of head and neck cancer patients. For this study, radiation survival curves were generated for Bl6, MG-63 and YAC-l cell lines using semiautomated MTT assay and Dye Exclusion Assay. Irradiation of 2, 4, 6, 8, 10Gy were delivered at room temperature at a dose rate of 210.2cGy/min using / sup 60/Co γ-ray Irradiator ALOORAOO 8. The viable cells were determined for each radiation dose and compared to control values. The obtained results were as follows: 1. There was significantly different absorbance at 10Gy on B16 cell line in MTT assay(P<0.05). 2. There was significantly different absorbance at 4, 6, 8, 10Gy on MG-63 cell line in MTT assay(P<0.05). 3. YAC-l cell line was more sensitive than B16 or MG-63 cell line to all doses of radiation(P<0.05). 4. There was significantly different absorbance among all tumor cell lines except between B16 and MG-63 cell line at ZGy in MTT assay(P<0.05). 5. Good correlation was obtained between MTT assay and DEA(P<0.05). The efficient of correlation of B16, MG-63 and YAC-l cell line was 0.845, 0.824 and 0.906, respectively.

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