• Biomedical Science Letters
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• v.8 no.1
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• pp.29-37
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• 2002

Listeria monocytogenes poses an increasing health risk, which in part is due to increasing health risk, consumption of ready-to-eat food products and the introduction of increasing numbers of food products from regions with different dietary habits. L. monocytogenes can be present in meat, shellfish, vegetables, unpasteurised milk and soft cheese and poses a risk if food containing these products is stored at refrigeration temperature and is not properly heated before consumption, as L. monocytogenes is psychrophilic. Amplified-fragment length polymorphism (AFLP) analysis is the method of genotypic techinique in which adaptor oligonucleotides are ligated to restriction enzyme fragments and then used as target sites for primers in a PCR amplification. The amplified fragments are electrophoretically separated to give strain-specific band profiles. Single-enzyme approach that did not require costly equipment or reagents for the fingerprinting of strains of Listeria monocytogenes was developed. Single-enzyme amplified fragment length polymorphism (SE-AFLP) analysis was used to perform species and strain identification of Salmonella, Shigella, Yersinia and E. coli. By careful selection of AFLP primers, it was possible to obtain reproducible and sensitive identification to strain level. The AFLP patterns of L. monocytogenes are divided by the kinds of specimens in which were isolated. SE-AFLP fragments can be analyzed using standard gel electrophoresis, and can be easily scored by visual inspection, due to the low complexity of the fingerprint obtained by this method. These features make SE-AFLP suitable for use in either field or laboratory applications.

• Aerospace Engineering and Technology
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• v.10 no.1
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• pp.20-29
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• 2011

There are a lot of electrical and electronic components and pneumatic parts sensitive to vibration and shock in the VEB assembly which KARI directly transports among the KSLV-I upper stage transportation items. So, the special attention in the transportation is required. The transportation container with the apparatus for controlling environment was made in order to meet the requirements. The transportation tests using the engineering model were performed 4 times to verify the performance of the transportation container and to conform the transportation conditions(transportation velocity, route, etc.). In this document, the previous transportation history of the VEB assembly, the overview and the result of the KSLV-I FM unit 2 VEB assembly transportation are described.

• Journal of Korean Society of Industrial and Systems Engineering
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• v.31 no.2
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• pp.11-18
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• 2008

Surface roughness is an important factor to evaluate machined parts in precision machining. This is the major measure of surface quality. This research sets up experiments to select the factors which affect surface roughness in the machining of inclined planes of aluminum. The levels of the selected experimental factors are chosen to evaluate the relationship between the surface roughness of the machined parts and machining parameters. This is to find out the optimal machining condition in the inclined planes. The objective of this research is to improve the surface roughness of the machined products by using the ANOVA analysis. The factors for the experiments are cutting speed, feed rate, cutting depth, and cutting width. The experimental levels of the factors are two for the cutting depth and width. For the cutting speed and feed rate, their levels are three because they are more sensitive for the surface roughness than the other two. The inclined planes are machined by 5-axis machining equipment.

• Geophysics and Geophysical Exploration
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• v.7 no.1
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• pp.70-79
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• 2004

We have examined the applicability of the triaxial drill-bit VSP method (TAD-VSP) to the geological survey of possible sites for a high-level radioactive waste disposal repository. The seismic energy generated by a drill bit is measured by a downhole multi-component detector, and the resulting signals are processed to image the geological structure deep underground. In order to apply the TAD-VSP method to civil-engineering-scale drilling, we have developed a small but highly sensitive and precise three-component downhole seismic measurement system, and recorded drill-bit signals at a granite quarry. We have successfully imaged discontinuities in the granite, possibly related to fractures, as highly reflective zones. The discontinuities imaged by the TAD-VSP method correlate well with the results of other borehole observations. In conclusion, the TAD-VSP method is usable in geological investigations for civil engineering because the equipment is compact and it is simple to acquire the drill-bit signal.

• Proceedings of the National Institute of Ecology of the Republic of Korea
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• v.1 no.1
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• pp.83-89
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• 2020

Methods for detecting the presence of genetically modified (GM) crops are evolving to comply with legislation and to enhance monitoring by biotechnology companies and regulators. In order to cover a broad range of detection methods for a new GM crop, conventional multiplex PCR methods are required. Based on the genetic information on three GM alfalfa varieties (J101, J163, and KK179), which were recently approved in South Korea, we developed a fast, reliable, and highly specific multiplex polymerase chain reaction (PCR) method with basic PCR equipment and inexpensive reagents. To validate and verify the newly developed multiplex PCR method, we applied a limit of detection assay and random reference material analysis. We also monitored the unintentional environmental release of GM alfalfa in South Korea by performing the multiplex PCR analysis with 91 feral alfalfa specimens collected from 2000 to 2018. Our methodology is a sensitive, simple, quick, and inexpensive tool for detecting and identifying three GM alfalfa varieties.

• Journal of Communications and Networks
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• v.13 no.2
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• pp.132-141
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• 2011

IEEE 802.15.4 networks are a feasible platform candidate for connecting all health-care-related equipment dispersed across a hospital room to collect critical time-sensitive data about patient health state, such as the heart rate and blood pressure. To meet the quality of service requirements of health-care systems, this paper proposes a multi-priority queue system that differentiates between various types of frames. The effect of the proposed system on the average delay and throughput is explored herein. By employing different contention window parameters, as in IEEE 802.11e, this multi-queue system prioritizes frames on the basis of priority classes. Performance under both saturated and unsaturated traffic conditions was evaluated using a novel analytical model that comprehensively integrates two legacy models for 802.15.4 and 802.11e. To improve the accuracy, our model also accommodates the transmission retries and deferment algorithms that significantly affect the performance of IEEE 802.15.4. The multi-queue scheme is predicted to separate the average delay and throughput of two different classes by up to 48.4% and 46%, respectively, without wasting bandwidth. These outcomes imply that the multi-queue system should be employed in health-care systems for prompt allocation of synchronous channels and faster delivery of urgent information. The simulation results validate these model's predictions with a maximum deviation of 7.6%.

• Proceedings of the KIEE Conference
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• 2000.07c
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• pp.1924-1926
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• 2000

This paper deals with the multiresolution analysis of wavelet transform for partial discharge(PD).PD is an electrical discharge that only partically bridges the insulation performance of electrical equipment in high voltage. PD signal is very sensitive and difficult to suppress strong noises such as narrow-band radio frequency noise and random noise. In recently, wavelet transform has become a powerful tool to analysis and process signals in various science and technology fields. In this paper, daubechies family is adopted for the research of the characteristics of PD signals. The results show that the kurtosis is increased with discharge process and skewness is decreased with discharge process, but when PD occured positive range then skewness is increased. Segment 7, 8, 9, 10, 11 values is increased with discharge process, so phase distribution is characterized by 210$\sim$330 ranges.

• Korean Journal of Air-Conditioning and Refrigeration Engineering
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• v.27 no.7
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• pp.375-379
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• 2015

A proper estimate of solar cell efficiency is of great importance for the feasibility analysis of solar cell power plant development. Since solar cell efficiency depends on temperature, several methods have been introduced to measure it by operating temperature modulation. However, the methods either rely on the external environment or need expensive equipment. In this paper, a thermoelectric module was used to control the operating temperature of crystalline silicon solar cells effectively and precisely over a wide range. The output characteristics of crystalline silicon solar cells in response to operating temperatures from $-5^{\circ}C$ to $100^{\circ}C$ were investigated experimentally. Their efficiencies decreased as the temperature rose, since the decrease in the open circuit voltage and fill factor exceeded the increase in the short circuit current. The maximum power temperature coefficient of the single crystalline solar cell was more sensitive to temperature change than that of the polycrystalline solar cell.

• Journal of the Earthquake Engineering Society of Korea
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• v.17 no.4
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• pp.181-186
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• 2013

The construction costs for nonstructural systems such as mechanical/electrical equipment, ceiling system, and piping system occupy a significant proportion of the total cost. These nonstructural systems can also cause considerable economic losses and loss of life during and after an earthquake. Therefore, reduction of seismic risk of nonstructural components has been emerging as a key aspect of research in recent year. The primary objective of this study was to evaluate the seismic performance of a single-story and multi-story piping system installed in low-rise building and to identify the seismic vulnerability of the current piping systems. The seismic performance evaluation of the piping systems was conducted with 5 different earthquakes to account for the ground motion uncertainty and the preliminary results demonstrated that the maximum displacements of each floor in the multi-story piping system increased linearly with increasing floor level in the building system. This study revealed that the current design piping systems are significantly sensitive to the effect of floor height, which stress the necessity to improve the seismic performance of the current piping systems by, for example, strengthening with seismic sway bracing using transverse/longitudinal bracing cables or hangers.

• Journal of the Institute of Electronics Engineers of Korea TC
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• v.41 no.9
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• pp.1-7
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• 2004

This thesis shows embodiment of lock system design for combination of usefulness, convenient of Barcode and cellular phone. This thesis consists of Lock system design using cellular phone and product introduction, including details from formation of barcode scanner to scanner design. When this research is applied to security system a general idea of physical key disappears and it is Possible that cellular phone becomes the center of security and we can expect that cellular phone use for payment will increase due to mutual direction service. After, we should study its application to various practical field and develop high sensitive equipment.