• Asian-Australasian Journal of Animal Sciences
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• v.25 no.2
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• pp.286-290
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• 2012

The majority of laboratory animals were transported from commercial breeders to a research facility by ground transportation. During the transportation, many biological functions and systems can be affected by stress. In this experiment, the change of body weight during the transportation was measured and the recovery periods from the transportation stress established based on the body weight changes. Total 676 laboratory animals which were aged between 3 to 9 wk old were studied. The transportation time taken from container packing to unpacking the container was approximately 24 h. The temperature of animal container was constantly maintained by air-conditioning and heating equipment. Rats were found to be more sensitive than mice. The body weight of rats was significantly decreased 3.71% (p<0.05) compared to the body weight of mice which decreased 0.9% There was no significant difference between the strains in the same species. When the changes of body weights were compared between delivery days, C57BL/6 mice showed the most variable changes compared to other species and strains. Consequently, C57BL/6 was more sensitive to stress than the other strains and the transportation process needs to be standardized to reduce between day variability. To establish the recovery periods from transportation stress, the body weight changes were measured during the acclimation period. Although the body weight of animals decreased during transportation, animals recovered their weight loss after the next day.

• Journal of Power System Engineering
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• v.20 no.3
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• pp.22-28
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• 2016

Energy consumption rapidly increases with industrial development. therefore an interest in the efficiency of various power converters is increasing. Especially, the field of high-efficiency small motors widely distributed for industrial and family use is considered a very important field in terms of efficient energy usage, and accordingly, in the field of small electric equipment, the use of BLDC that allows high-efficiency drive in an inductor gradually increases. However, for the BLDC drive, information on the position of the rotor is essential. Both methods using a magnetic encoder and an optical encoder to detect the information on the position of the rotor obtain the information by three position sensitive devices, and if any one of these position sensitive devices fails to function, no positional information can be obtained, so there is a limitation in implementing a position sensor with high reliability. In the paper, proposes a new type of encoder that can obtain the positional information on the position of the rotor using a single position sensor in order to overcome the issues that it has to use numerous signal flows and that the reliability is reduced for the acquisition of positional information generated by using multiple position detectors. The encoder in the proposed method replaced the function of generating positional information from multiple sensors with the shape of the encoder plate and the capture function of MICOM. In order to verify the validity of the position detection technique by the proposed encoder, a prototype was produced, and an experiment using the capture function of DSP was conducted through this.

• The Plant Pathology Journal
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• v.36 no.1
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• pp.76-86
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• 2020

Cucumber mosaic virus (CMV) is damaging to the growth and quality of lettuce crops in Lanzhou, China. Recently, however, for the first time an isolate of lettuce necrotic yellows virus (LNYV) has been detected in lettuce crops in China, and there is concern that this virus may also pose a threat to lettuce production in China. Consequently, there is a need to develop a rapid and efficient detection method to accurately identify LNYV and CMV infections and help limit their spread. Reverse transcription loop-mediated isothermal amplification (RT-LAMP) assays were developed to detect the nucleoprotein (N) and coat protein (CP) genes of LNYV and CMV, respectively. RT-LAMP amplification products were visually assessed in reaction tubes separately using green fluorescence and gel electrophoresis. The assays successfully detected both viruses in infected plants without cross reactivity recorded from either CMV or LNYV or four other related plant viruses. Optimum LAMP reactions were conducted in betaine-free media with 6 mM Mg²⁺ at 65℃ for LNYV and 60℃ for 60 min for CMV, respectively. The detection limit was 3.5 pg/ml and 20 fg/ml using RT-LAMP for LNYV and CMV plasmids, respectively. Detection sensitivity for both RT-LAMP assays was greater by a factor of 100 compared to the conventional reverse transcription polymerase chain reaction assays. This rapid, specific, and sensitive technique should be more widely applied due to its low cost and minimal equipment requirements.

• Smart Structures and Systems
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• v.30 no.6
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• pp.661-671
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• 2022

Stayed cables are the key components for transmitting loads in cable-stayed bridges. Therefore, it is very important to evaluate the cable force condition to ensure bridge safety. An online condition assessment and anomaly localization method is proposed for cables based on the spatiotemporal correlation of grouped cable forces. First, an anomaly sensitive feature index is obtained based on the distribution characteristics of grouped cable forces. Second, an adaptive anomaly detection method based on the k-nearest neighbor rule is used to perform dissimilarity measurements on the extracted feature index, and such a method can effectively remove the interference of environment factors and vehicle loads on online condition assessment of the grouped cable forces. Furthermore, an online anomaly isolation and localization method for stay cables is established, and the complete decomposition contributions method is used to decompose the feature matrix of the grouped cable forces and build an anomaly isolation index. Finally, case studies were carried out to validate the proposed method using an in-service cable-stayed bridge equipped with a structural health monitoring system. The results show that the proposed approach is sensitive to the abnormal distribution of grouped cable forces and is robust to the influence of interference factors. In addition, the proposed approach can also localize the cables with abnormal cable forces online, which can be successfully applied to the field monitoring of cables for cable-stayed bridges.

• Journal of Drive and Control
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• v.21 no.2
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• pp.53-64
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• 2024

Global population growth has resulted in an increased demand for food production. Simultaneously, aging rural communities have led to a decrease in the workforce, thereby increasing the demand for automation in agriculture. Drones are particularly useful for unmanned pest control fields. However, the current method of uniform spraying leads to environmental damage due to overuse of pesticides and drift by wind. To address this issue, it is necessary to enhance spraying performance through precise performance evaluation. Therefore, as a foundational study aimed at optimizing drone-based pest control technologies, this research evaluated water-sensitive paper (WSP) via density map estimation using convolutional neural networks (CNN) with a encoder-decoder structure. To achieve more accurate estimation, this study implemented multi-task learning, incorporating an additional classifier for image segmentation alongside the density map estimation classifier. The proposed model in this study resulted in a R-squared (R²) of 0.976 for coverage area in the evaluation data set, demonstrating satisfactory performance in evaluating WSP at various density levels. Further research is needed to improve the accuracy of spray result estimations and develop a real-time assessment technology in the field.

• Journal of Animal Science and Technology
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• v.66 no.4
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• pp.781-791
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• 2024

Bovine viral diarrhea (BVD) is a single-stranded, positive-sense ribonucleic acid (RNA) virus belonging to the genus Pestivirus of the Flaviviridae family. BVD frequently causes economic losses to farmers. Among bovine viral diarrhea virus (BVDV) strains, BVDV-1b is predominant and widespread in Hanwoo calves. Reverse-transcription polymerase chain reaction (RT-PCR) is an essential method for diagnosing BVDV-1b and has become the gold standard for diagnosis in the Republic of Korea. However, this diagnostic method is time-consuming and requires expensive equipment. Therefore, Clustered regularly interspaced short palindromic repeats-Cas (CRISPR-Cas) systems have been used for point-of-care (POC) testing of viruses. Developing a sensitive and specific method for POC testing of BVDV-1b would be advantageous for controlling the spread of infection. Thus, this study aimed to develop a novel nucleic acid detection method using the CRISPR-Cas13 system for POC testing of BVDV-1b. The sequence of the BVD virus was extracted from National Center for Biotechnology Information (NC_001461.1), and the 5' untranslated region, commonly used for detection, was selected. CRISPR RNA (crRNA) was designed using the Cas13 design program and optimized for the expression and purification of the LwCas13a protein. Madin Darby bovine kidney (MDBK) cells were infected with BVDV-1b, incubated, and the viral RNA was extracted. To enable POC viral detection, the compatibility of the CRISPR-Cas13 system was verified with a paper-based strip through collateral cleavage activity. Finally, a colorimetric assay was used to evaluate the detection of BVDV-1b by combining the previously obtained crRNA and Cas13a protein on a paper strip. In conclusion, the CRISPR-Cas13 system is highly sensitive, specific, and capable of nucleic acid detection, making it an optimal system for the early point-of-care testing of BVDV-1b.

• Journal of Electrical Engineering and Technology
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• v.13 no.6
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• pp.2412-2420
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• 2018

The thermal degradation of low density polyethylene (LDPE) will accelerate the production of carbonyl groups (C=O), which can act as the induced dipoles under high voltage. In this paper, we researched the dielectric properties and space charge behavior of LDPE after thermal aging, which can help us to understand the correlation between carbonyl groups (C=O) and electrical properties of LDPE. The spectra results show that LDPE exhibit obvious thermooxidative reactions when the aging time is 35 days and the productions mainly contain carboxylic acid, carboxylic eater and carboxylic anhydride, whose amount increase with the increasing of aging time. The dielectric properties show that the real permittivity of LDPE is inversely proportional to temperature before aging and subsequently become proportional to temperature after thermal aging. Furthermore, both the real and imaginary permittivity increase sharply with the increasing of aging time. The fitting results of imaginary permittivity show that DC conductivity become more sensitive about temperature after thermal aging. On this basis, the active energies of materials calculated from DC conductivity increase first and then decrease with the increasing of aging time. In addition, the space charge results show that the heterocharges accumulated near electrodes in LDPE change to the homocharges after thermal aging and the mean volume charge density increase with the increasing of aging time. It is considered that the overlaps caused by electrical potential area is the main reason for the increase of DC conductivity.

• Journal of Advanced Navigation Technology
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• v.19 no.5
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• pp.433-439
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• 2015

Recently, Interest in power the quality was increased because of increasing the use of sensitive load equipment into an electrical disturbance such as computer, Electricity, Electronics, Telecommunications and semiconductor device. In addition, To enhance power quality, the instantaneous voltage drop occurred in precision load equipment is a need for proper compensation. In order to solve the problem, The developed dynamic voltage restorer (DVR) using an electric double layer capacitor (EDLC) has been applied. In this paper, We will do study to apply hybrid capacitors that have high energy density to the same size compared to the EDLC to DVR. Also, As a emergency luminaires of emergency power supply that we can support more than 10 years of life was confirmed the applicability of hybrid capacitor.

• Journal of the Computational Structural Engineering Institute of Korea
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• v.20 no.4
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• pp.463-468
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• 2007

Some network equipments made in Korea were exposed to severe earthquake in Japan several years ago. More than a hundred slim base transfer network stations had been seized with the severe earthquake at Nigata and it was reported that less than fifteen sets showed blackout by interruption of electricity, not by the structural failure. The purpose of this paper is to check the structural safety of the network equipments by performing table test, and the static and dynamic finite element analysis. For the dynamic test, the station weighing 200 kg was subjected to the Zone 3 earthquake loading of GR-63-CORE on the shaking table to obtain the dynamic responses to compare with the analysis results. It is shown that the FE analysis results are a little bit larger than that of the experimental values. And the sensitivity analysis and optimization for the natural frequency is performed and it is found that the first natural frequency is sensitive to small design change as shown in the results. And the dynamic response of optimized design is less than the original design.

• Journal of the Korean Society of Radiology
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• v.15 no.6
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• pp.795-801
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• 2021

The designed drone-based unmanned remote radiation detection module was developed according to the needs of the nuclear power plant decommissioning workshop. Using the Geiger-Mueller tube sensitive to low-level radiation measurement, It was manufactured to measure the amount of radiation leaking into and out of the containment vessel. The drone-based radiation detection module weighs less than 200g, It can be operated inside and outside the containment vessel of a nuclear power plant. To check the performance of the designed equipment, a performance evaluation test was conducted with reference to the international standard (IEC-60864). The stability of the radiation detection module designed to meet the needs of the field the statistical rate of change by repeated measurements in the rate of change experiment to evaluate the measurement accuracy was ±4.6%. The accuracy ±7.3% in the linearity experiment to evaluate the dose rate dependence, the linear The figure satisfies the international performance evaluation standard of ±3.5%. The radiation detection module developed in this study is a customized equipment for a nuclear power plant dismantling workshop. It will be helpful for accurate measurement of space dose rate and safety management of radiation worksites in sites with a lot of radiation dust.